##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049389_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5922853
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81776316244891	31.0	31.0	34.0	30.0	34.0
2	31.937360592943975	33.0	31.0	34.0	30.0	34.0
3	31.987471071795973	33.0	31.0	34.0	30.0	34.0
4	35.73432921600452	37.0	35.0	37.0	33.0	37.0
5	35.77778952136749	37.0	35.0	37.0	35.0	37.0
6	35.8597878421092	37.0	35.0	37.0	35.0	37.0
7	35.810174252172054	37.0	35.0	37.0	35.0	37.0
8	35.82369982844416	37.0	35.0	37.0	35.0	37.0
9	37.56110475812923	39.0	37.0	39.0	35.0	39.0
10-11	37.51984575676621	39.0	37.0	39.0	35.0	39.0
12-13	37.4639391691808	39.0	37.0	39.0	34.5	39.0
14-15	38.76499231029371	40.0	38.0	41.0	35.0	41.0
16-17	38.79276313290234	40.0	38.0	41.0	35.0	41.0
18-19	38.85855034727352	40.0	38.0	41.0	35.0	41.0
20-21	38.887002851497414	40.0	38.0	41.0	35.0	41.0
22-23	38.856059824547394	40.0	38.0	41.0	35.0	41.0
24-25	38.83449251568459	40.0	38.0	41.0	35.0	41.0
26-27	38.728168418159285	40.0	38.0	41.0	34.5	41.0
28-29	38.6493526852684	40.0	38.0	41.0	34.0	41.0
30-31	38.54986279416356	40.0	38.0	41.0	34.0	41.0
32-33	38.418755032414275	40.0	38.0	41.0	34.0	41.0
34-35	38.31749319120363	40.0	38.0	41.0	34.0	41.0
36-37	38.206464857392206	40.0	38.0	41.0	34.0	41.0
38-39	38.113590021565614	40.0	38.0	41.0	33.5	41.0
40-41	38.004643201511165	40.0	38.0	41.0	33.0	41.0
42-43	37.866951281755604	40.0	37.5	41.0	33.0	41.0
44-45	37.69843950204403	40.0	37.0	41.0	33.0	41.0
46-47	37.48433162193963	40.0	37.0	41.0	33.0	41.0
48-49	37.2841400926209	40.0	37.0	41.0	32.0	41.0
50-51	37.139151942484474	40.0	37.0	41.0	32.0	41.0
52-53	37.002151496922174	40.0	36.5	41.0	31.0	41.0
54-55	36.860609574473656	40.0	36.0	41.0	31.0	41.0
56-57	36.716078129914756	39.0	36.0	41.0	31.0	41.0
58-59	36.525536848542416	39.0	35.0	41.0	31.0	41.0
60-61	36.34968384324244	39.0	35.0	41.0	31.0	41.0
62-63	36.14834185484597	39.0	35.0	41.0	30.5	41.0
64-65	35.920841442460244	38.5	35.0	40.5	30.0	41.0
66-67	35.65280499110817	38.0	35.0	40.0	30.0	41.0
68-69	35.36525784111137	37.0	35.0	40.0	29.5	41.0
70-71	35.035182959968786	37.0	34.5	39.5	29.0	41.0
72-73	34.74082861755981	36.0	34.0	39.0	29.0	41.0
74-75	34.43611381204295	36.0	34.0	39.0	29.0	41.0
76-77	34.10910020897024	35.0	34.0	38.5	29.0	40.0
78-79	33.774275758658874	35.0	34.0	37.0	29.0	39.0
80-81	33.45687762299689	35.0	34.0	37.0	29.0	39.0
82-83	33.15625273833404	35.0	34.0	36.5	28.0	39.0
84-85	32.88393406015648	35.0	34.0	36.0	28.0	37.5
86-87	32.649969533263786	35.0	33.0	36.0	27.5	37.0
88-89	32.42361713181131	35.0	33.0	35.0	27.0	37.0
90-91	32.230007987704575	35.0	33.0	35.0	27.0	36.0
92-93	32.04829927401541	35.0	33.0	35.0	26.5	36.0
94-95	31.88977263153416	35.0	33.0	35.0	26.0	36.0
96-97	31.690535794151906	35.0	33.0	35.0	26.0	36.0
98-99	31.54386036594189	35.0	33.0	35.0	25.5	35.0
100	31.431792752580556	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11080734803715231
1101	2	0.19100849359405458
1101	3	0.3324658171868151
1101	4	0.17471890175013982
1101	5	0.14409299285627952
1101	6	0.1296156407553184
1101	7	0.08874906255373105
1101	8	0.12081307660706386
1101	9	0.2311881646650349
1101	10-11	0.24005251570421393
1101	12-13	0.1656646311974015
1101	14-15	0.314462139983533
1101	16-17	0.236151145624369
1101	18-19	0.2533663632120451
1101	20-21	0.23285897008827305
1101	22-23	0.22713756343623004
1101	24-25	0.24050369977231156
1101	26-27	0.22974828852077422
1101	28-29	0.2716566258180464
1101	30-31	0.24608058448223602
1101	32-33	0.32219467480385333
1101	34-35	0.3553089417208142
1101	36-37	0.3964686839471696
1101	38-39	0.4026505684786983
1101	40-41	0.4092050894572026
1101	42-43	0.42465892611681255
1101	44-45	0.4436689801701803
1101	46-47	0.4937063960642689
1101	48-49	0.5755832208382685
1101	50-51	0.473535005951625
1101	52-53	0.5265628273262379
1101	54-55	0.5211301928591467
1101	56-57	0.488046000837528
1101	58-59	0.5245891879755789
1101	60-61	0.5429348509881038
1101	62-63	0.49278671206348434
1101	64-65	0.4235219914392161
1101	66-67	0.4440818144112626
1101	68-69	0.4869191695919639
1101	70-71	0.5169543528436478
1101	72-73	0.440148467366015
1101	74-75	0.4471305014900935
1101	76-77	0.40049972041217075
1101	78-79	0.42533921518724327
1101	80-81	0.49385726764884197
1101	82-83	0.4495228768956778
1101	84-85	0.4797585423474473
1101	86-87	0.413633915955522
1101	88-89	0.46419332193570284
1101	90-91	0.5311636481826838
1101	92-93	0.5135091542888759
1101	94-95	0.5528332818961736
1101	96-97	0.5669114290496431
1101	98-99	0.6090052853610608
1101	100	0.6504387156845404
1102	1	0.18151267459850118
1102	2	0.15560647071070832
1102	3	0.3157971322744686
1102	4	0.21279441002598531
1102	5	0.17115315853891389
1102	6	0.16463316943373485
1102	7	0.15767495895233452
1102	8	0.18384922295772554
1102	9	0.11368102443082506
1102	10-11	0.19579484180044915
1102	12-13	0.2063420814304635
1102	14-15	0.2611915498595394
1102	16-17	0.18876441644238895
1102	18-19	0.2407476118448173
1102	20-21	0.1940980174247784
1102	22-23	0.18168420165851273
1102	24-25	0.20439685519575335
1102	26-27	0.312254769911263
1102	28-29	0.2670845018764183
1102	30-31	0.23979330444801406
1102	32-33	0.26840348924953616
1102	34-35	0.30175256126101146
1102	36-37	0.3487750157911691
1102	38-39	0.3282517833153733
1102	40-41	0.32967499900912145
1102	42-43	0.3490713773798575
1102	44-45	0.33049101525379143
1102	46-47	0.27869391944126676
1102	48-49	0.213441799909873
1102	50-51	0.31899255144834626
1102	52-53	0.3408099287512485
1102	54-55	0.2571627212130423
1102	56-57	0.31063038160355916
1102	58-59	0.2281275085105392
1102	60-61	0.2746305205812831
1102	62-63	0.278633201414074
1102	64-65	0.27263703978571385
1102	66-67	0.21668671809800344
1102	68-69	0.2873561412517418
1102	70-71	0.2615767556405828
1102	72-73	0.32897578902640134
1102	74-75	0.29203021523046857
1102	76-77	0.28028214217938086
1102	78-79	0.349549029063283
1102	80-81	0.34135669959550086
1102	82-83	0.3611204496270517
1102	84-85	0.3281346305898083
1102	86-87	0.33096661136372063
1102	88-89	0.31753887240331125
1102	90-91	0.37252943828046625
1102	92-93	0.4113271930006661
1102	94-95	0.4012571654474435
1102	96-97	0.4330591596642712
1102	98-99	0.3955301184325073
1102	100	0.39534913256821014
1103	1	0.1108992437082641
1103	2	0.1356441475368797
1103	3	0.282033195538709
1103	4	0.1835719753337841
1103	5	0.1433996822900454
1103	6	0.11652819417368931
1103	7	0.12295699127883353
1103	8	0.13519265415924764
1103	9	0.17625860456825393
1103	10-11	0.19189215356816902
1103	12-13	0.180820759228844
1103	14-15	0.24212922768833778
1103	16-17	0.18858897763625038
1103	18-19	0.23853174328712612
1103	20-21	0.22499306297518729
1103	22-23	0.22462180201647186
1103	24-25	0.23324596963236388
1103	26-27	0.2434491085125785
1103	28-29	0.26260573722209557
1103	30-31	0.24694715931883593
1103	32-33	0.2576009123444223
1103	34-35	0.24486553921790488
1103	36-37	0.25995542042561937
1103	38-39	0.23643765439479836
1103	40-41	0.2820194303947332
1103	42-43	0.33372327624316966
1103	44-45	0.2939960492844236
1103	46-47	0.2560118757943428
1103	48-49	0.1978476676526384
1103	50-51	0.18339930319808673
1103	52-53	0.19788251939358048
1103	54-55	0.20872396263980875
1103	56-57	0.1631212556086865
1103	58-59	0.21028131528998983
1103	60-61	0.2166200402166112
1103	62-63	0.19313444689334602
1103	64-65	0.24622165513804362
1103	66-67	0.16964679199433874
1103	68-69	0.16286709028874924
1103	70-71	0.18690785166583623
1103	72-73	0.12799745154011077
1103	74-75	0.1300217790699989
1103	76-77	0.1291113459108999
1103	78-79	0.17345982346173372
1103	80-81	0.17191547856880618
1103	82-83	0.15165281069455716
1103	84-85	0.1646937344276651
1103	86-87	0.17542630530902414
1103	88-89	0.15289566991855708
1103	90-91	0.10582417658234533
1103	92-93	0.20355242159793363
1103	94-95	0.1821037261444225
1103	96-97	0.2538582014158628
1103	98-99	0.2895095121220592
1103	100	0.30940790093226767
1104	1	0.07954849390437246
1104	2	0.1304167802100622
1104	3	0.25944202553227314
1104	4	0.15790579984953723
1104	5	0.11507024540096467
1104	6	0.15521861395234282
1104	7	0.1618083233265324
1104	8	0.17648985821035268
1104	9	0.14596084932404807
1104	10-11	0.18736384411263174
1104	12-13	0.2117284040917582
1104	14-15	0.26543887173421865
1104	16-17	0.23283608142562429
1104	18-19	0.253909961902977
1104	20-21	0.30139440957639607
1104	22-23	0.2735066681369318
1104	24-25	0.2891302506486255
1104	26-27	0.32908989754649554
1104	28-29	0.35866737049472164
1104	30-31	0.3656639043061105
1104	32-33	0.39171445123090365
1104	34-35	0.3844452773953222
1104	36-37	0.35364839146257765
1104	38-39	0.3627489057023965
1104	40-41	0.40778011581786444
1104	42-43	0.3929487510277525
1104	44-45	0.422935430932192
1104	46-47	0.45676141432434036
1104	48-49	0.4140747939487852
1104	50-51	0.3906514522274094
1104	52-53	0.39294211434764037
1104	54-55	0.424388288866723
1104	56-57	0.4219796979578021
1104	58-59	0.5099308386358175
1104	60-61	0.4816557257827654
1104	62-63	0.4930510476619858
1104	64-65	0.44709466588544444
1104	66-67	0.4591582496357631
1104	68-69	0.37857250269897946
1104	70-71	0.4224438988469146
1104	72-73	0.44936482398253474
1104	74-75	0.4743899840573178
1104	76-77	0.3864900860131897
1104	78-79	0.4275671908050924
1104	80-81	0.47201262833459623
1104	82-83	0.4440355866993144
1104	84-85	0.440135011133421
1104	86-87	0.41737030830809374
1104	88-89	0.4156726561694697
1104	90-91	0.3669913164305143
1104	92-93	0.486251630139229
1104	94-95	0.5159021468938185
1104	96-97	0.517587289834001
1104	98-99	0.5349235710406468
1104	100	0.5468428796864906
1105	1	0.15015021035530296
1105	2	0.13722290554083116
1105	3	0.22180638963956767
1105	4	0.12670758504126667
1105	5	0.11592509865035083
1105	6	0.10384471786986893
1105	7	0.11667683607687707
1105	8	0.12291958278681392
1105	9	0.1333299983753662
1105	10-11	0.17200532297366067
1105	12-13	0.10814039343608073
1105	14-15	0.19481294736259258
1105	16-17	0.16240698309896828
1105	18-19	0.15207221599892762
1105	20-21	0.15575230348336788
1105	22-23	0.12258320176960069
1105	24-25	0.1495266817057157
1105	26-27	0.1355877696787786
1105	28-29	0.13714388360599372
1105	30-31	0.1330029797468839
1105	32-33	0.17693694638064272
1105	34-35	0.11363314781923606
1105	36-37	0.1391399566359368
1105	38-39	0.22234439966347708
1105	40-41	0.22879515779604986
1105	42-43	0.2273868591273427
1105	44-45	0.20728787494670797
1105	46-47	0.24823458187084668
1105	48-49	0.2903949555846168
1105	50-51	0.3107766288512721
1105	52-53	0.35406723852209865
1105	54-55	0.3168188119051365
1105	56-57	0.27586108805164855
1105	58-59	0.273458112751257
1105	60-61	0.23221823417647158
1105	62-63	0.3073585966895678
1105	64-65	0.2510610587173332
1105	66-67	0.310760722968908
1105	68-69	0.2570907199614396
1105	70-71	0.2651306021897426
1105	72-73	0.2610358050109767
1105	74-75	0.28632909874674084
1105	76-77	0.23620059608133914
1105	78-79	0.2484430617942266
1105	80-81	0.2847701043226252
1105	82-83	0.2586153749779072
1105	84-85	0.24067853362881664
1105	86-87	0.21836887030390528
1105	88-89	0.24151375700190414
1105	90-91	0.2445281143361484
1105	92-93	0.31535875074489894
1105	94-95	0.3011739156717077
1105	96-97	0.34203355083650777
1105	98-99	0.3187423865733514
1105	100	0.29479832532602046
1106	1	0.09127998830375716
1106	2	0.10439280774485127
1106	3	0.23966130894908844
1106	4	0.12966884992020056
1106	5	0.12398310330070217
1106	6	0.11318609710257732
1106	7	0.1477951310869443
1106	8	0.09215078500923113
1106	9	0.12963305636863254
1106	10-11	0.1283390970049183
1106	12-13	0.11128136541532996
1106	14-15	0.1556219619878405
1106	16-17	0.1523054189249322
1106	18-19	0.1689323267906886
1106	20-21	0.17270770065414354
1106	22-23	0.14717248243776737
1106	24-25	0.13889772835821645
1106	26-27	0.1846044972260188
1106	28-29	0.20413726630659568
1106	30-31	0.24537814801279012
1106	32-33	0.2001463960369776
1106	34-35	0.2637875502067999
1106	36-37	0.3079199684300491
1106	38-39	0.2864950341091017
1106	40-41	0.2566475573861169
1106	42-43	0.27951385273519946
1106	44-45	0.2507674747059667
1106	46-47	0.31400153666085373
1106	48-49	0.30493090345576945
1106	50-51	0.2659775663589343
1106	52-53	0.28735652736163075
1106	54-55	0.29133903429820407
1106	56-57	0.2585782854658447
1106	58-59	0.2980422135744405
1106	60-61	0.2585072250009972
1106	62-63	0.2531512913246132
1106	64-65	0.23635900162949497
1106	66-67	0.29394938114493385
1106	68-69	0.2856491469683746
1106	70-71	0.27632766879104054
1106	72-73	0.30691192108099585
1106	74-75	0.23432247486976365
1106	76-77	0.21872580402776975
1106	78-79	0.2815034887652388
1106	80-81	0.24791629156459294
1106	82-83	0.35255814395226537
1106	84-85	0.2530872475574242
1106	86-87	0.2628189921282953
1106	88-89	0.31447176597827564
1106	90-91	0.298090557462217
1106	92-93	0.3003384652323504
1106	94-95	0.3145108021636567
1106	96-97	0.32277350845159347
1106	98-99	0.28864791100767206
1106	100	0.2900625556061165
1107	1	0.118900396348387
1107	2	0.07204497711691715
1107	3	0.1558368746500136
1107	4	0.16252241330269612
1107	5	0.11408302314608676
1107	6	0.13579684176209383
1107	7	0.10415429371509077
1107	8	0.137748439390613
1107	9	0.08982249615832671
1107	10-11	0.09796073224772073
1107	12-13	0.10544126180059266
1107	14-15	0.1651924327718035
1107	16-17	0.16156629435914027
1107	18-19	0.09271363918823994
1107	20-21	0.13771097433215118
1107	22-23	0.09797854866902611
1107	24-25	0.1274143359311779
1107	26-27	0.21396823117970598
1107	28-29	0.20225634246432378
1107	30-31	0.1907858231496391
1107	32-33	0.20819420011091694
1107	34-35	0.16579751213951255
1107	36-37	0.18709194958903197
1107	38-39	0.15889397500406943
1107	40-41	0.19902584873803875
1107	42-43	0.21155363986976994
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2216	74-75	-0.7081042871241365
2216	76-77	-0.6900795186310305
2216	78-79	-0.8969164423283047
2216	80-81	-1.0306422158604391
2216	82-83	-0.8709268101432883
2216	84-85	-0.9377380035395646
2216	86-87	-0.8042252328068145
2216	88-89	-1.1727831945565086
2216	90-91	-0.9635375733671196
2216	92-93	-1.0287615686234233
2216	94-95	-0.9973259399814758
2216	96-97	-1.0602262459280816
2216	98-99	-1.2160246837387092
2216	100	-1.1883855886327588
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	381.0
3	23.0
4	40.0
5	84.0
6	268.0
7	728.0
8	1683.0
9	2881.0
10	4458.0
11	6309.0
12	8851.0
13	12375.0
14	15066.0
15	19503.0
16	24066.0
17	32183.0
18	32032.0
19	18270.0
20	14235.0
21	14089.0
22	14894.0
23	16660.0
24	19814.0
25	24593.0
26	31409.0
27	40594.0
28	53203.0
29	69993.0
30	91556.0
31	118702.0
32	153215.0
33	200588.0
34	274130.0
35	398229.0
36	646808.0
37	1183165.0
38	1674751.0
39	700387.0
40	2637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24639560148527	20.690041320527303	14.634989622575223	23.428573455412206
2	15.460403964103111	20.475672788097224	39.78972633627747	24.2741969115222
3	17.692064955858267	25.12220039903067	31.16555484324868	26.02017980186238
4	12.610402968301738	17.32750865799696	38.22016030126048	31.84192807244083
5	12.597746390126515	37.66696556541248	35.857955616997415	13.87733242746359
6	30.665711271240397	38.32018116944655	17.20110223907296	13.813005320240093
7	26.315966308804217	32.99629418457625	22.527876346078486	18.159863160541043
8	24.64113649045763	36.57784880280341	20.27514036869417	18.50587433804479
9	24.437277102774626	16.59776124783107	21.80808809538241	37.156873554011895
10-11	23.648257013976203	27.034910371741454	28.865995830050146	20.450836784232195
12-13	24.282255453193084	24.583273299239035	29.164416619779548	21.970054627788336
14-15	21.60091320986057	26.64565986832847	26.777319580144304	24.97610734166666
16-17	20.63967314400678	29.961726215389778	27.5423431917017	21.856257448901736
18-19	20.5037387819026	28.311380084406977	30.41204899363085	20.772832140059563
20-21	21.536196386553467	27.560475747357753	30.26234247552635	20.64098539056243
22-23	21.195452596915707	27.40914724711216	30.325503604428473	21.069896551543657
24-25	21.13839408385516	27.517272742391384	30.24743098479641	21.096902188957046
26-27	20.888955861179948	27.507512222794862	30.340760926226785	21.262770989798405
28-29	20.916549577211676	27.48216137510126	30.46477675675548	21.136512290931584
30-31	21.219377392692927	27.808653958089945	30.065962326754658	20.90600632246247
32-33	20.891768138257998	27.569831364683157	30.401209554875702	21.137190942183143
34-35	21.032837144884883	27.325103005723346	30.39651262512873	21.24554722426305
36-37	21.343791367883668	27.659428621018627	29.858928472990144	21.13785153810756
38-39	21.223514979075034	27.81596429679128	29.906012819211902	21.05450790492179
40-41	21.10423594465938	27.600208784745643	30.029446145531548	21.26610912506343
42-43	21.1038851951698	27.57091769001136	29.89339363346867	21.431803481350176
44-45	21.258158259427248	27.720278069680184	29.677981749271293	21.34358192162128
46-47	21.326479418205125	27.905928968070825	29.29756627876458	21.470025334959466
48-49	21.37695550507079	27.897943852048996	29.087862772978013	21.637237869902208
50-51	21.43928966239463	27.90995164041438	28.890219899566382	21.76053879762461
52-53	21.369093733150414	27.98321944732807	28.93173061394776	21.715956205573757
54-55	21.494953291257097	28.28351796243023	28.709987074570297	21.511541671742375
56-57	21.3455004081674	28.003255208882937	28.800882688383815	21.850361694565844
58-59	21.23802812097849	27.68492908298353	29.104062950902932	21.97297984513505
60-61	21.41588566425178	28.021841560049687	28.797280762580503	21.764992013118036
62-63	21.239160308320752	28.039887600326402	28.82590190134958	21.89505019000326
64-65	21.266499741677105	27.818648886847818	28.971191088029013	21.94366028344606
66-67	21.226330156460193	27.83941250288612	28.79780980211481	22.13644753853888
68-69	21.575202131577033	27.965653123016654	28.599497091807507	21.85964765359881
70-71	21.54318615579325	27.80114688533713	28.665751337537632	21.98991562133199
72-73	21.36788386744172	27.940899747559573	28.71439585796104	21.976820527037667
74-75	21.43609986147563	27.9109429019408	28.592868776502	22.060088460081573
76-77	21.446674581836575	27.90238373581162	28.463638168678212	22.18730351367359
78-79	21.484744410820227	27.96513013011576	28.502009827021656	22.04811563204235
80-81	21.378369135394017	27.824521346373487	28.58276754925409	22.214341968978406
82-83	21.288464848702755	27.84556663306466	28.666163579269572	22.199804938963016
84-85	21.361429816283657	27.71698305306245	28.51751821773415	22.404068912919744
86-87	21.3904732430089	27.866127627480463	28.46377968516539	22.27961944434525
88-89	21.23467787726836	27.719942182038782	28.527264552291538	22.518115388401323
90-91	21.154518440463114	27.603418965623348	28.580942863845916	22.66111973006762
92-93	21.23515255347926	27.769654127805193	28.450661848363417	22.54453147035213
94-95	21.093049297068326	27.543264607039042	28.588628959055985	22.775057136836647
96-97	21.101460516877246	27.56869438893498	28.557607569634847	22.77223752455293
98-99	21.053815255619508	27.625260228917025	28.46924613392271	22.851678381540758
100	21.118515692008454	27.53133674165423	28.454926419439587	22.89522114689773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	855.0
1	592.5
2	642.5
3	944.0
4	618.5
5	404.0
6	967.0
7	2635.5
8	4522.5
9	5230.0
10	4100.5
11	2707.5
12	2593.0
13	2943.0
14	3342.5
15	3847.5
16	4437.0
17	5074.0
18	5899.0
19	7080.0
20	8729.0
21	10988.5
22	14130.5
23	18795.5
24	25282.5
25	33501.0
26	44781.0
27	59042.5
28	75197.5
29	93749.0
30	111394.5
31	126912.0
32	140466.0
33	152313.5
34	164403.5
35	175279.0
36	184861.0
37	194305.0
38	202514.5
39	206641.0
40	208852.0
41	211013.5
42	211335.0
43	210752.0
44	208074.5
45	203623.0
46	199351.0
47	192609.5
48	185148.5
49	180562.5
50	176545.0
51	169380.5
52	158171.0
53	148020.5
54	140601.0
55	133378.0
56	123690.5
57	112467.0
58	103652.0
59	95322.0
60	83835.5
61	71202.5
62	60802.0
63	50839.5
64	41115.0
65	33220.0
66	26608.5
67	20954.5
68	16205.5
69	12276.0
70	9160.5
71	6668.5
72	4815.0
73	3413.5
74	2449.5
75	1665.0
76	1051.0
77	680.0
78	431.0
79	288.5
80	160.5
81	66.0
82	43.5
83	27.0
84	11.0
85	7.5
86	4.5
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03898459070316282
2	0.0
3	0.0
4	8.441877588385192E-5
5	0.0
6	0.0
7	0.0
8	9.792578002526824E-4
9	0.0
10-11	0.0
12-13	0.04399906599066362
14-15	1.4351191900254827E-4
16-17	0.0
18-19	2.5325632765155572E-5
20-21	5.909314311869635E-5
22-23	0.0
24-25	1.6039567417931865E-4
26-27	9.286065347223711E-5
28-29	5.233964104798819E-4
30-31	4.220938794192596E-5
32-33	2.19488817298015E-4
34-35	4.220938794192596E-5
36-37	1.097444086490075E-4
38-39	4.0521012424248916E-4
40-41	1.097444086490075E-4
42-43	6.078151863637338E-4
44-45	3.5455885871217807E-4
46-47	1.6039567417931865E-4
48-49	9.20164657133986E-4
50-51	5.993733087753485E-4
52-53	7.513271053662821E-4
54-55	5.487220432450375E-4
56-57	6.415826967172746E-4
58-59	2.532563276515557E-4
60-61	2.7014008282832616E-4
62-63	0.001013025310606223
64-65	5.571639208334227E-4
66-67	0.0016968173952654237
68-69	0.0017221430280305791
70-71	4.896289001263412E-4
72-73	0.0011734209847855418
74-75	0.0011565372296087712
76-77	8.273040036617488E-4
78-79	0.0034442860560611583
80-81	0.002912447767992891
82-83	0.0015448635986744902
84-85	0.003207913483586373
86-87	0.0015617473538512604
88-89	0.0021273531522730685
90-91	0.0031150528301141355
92-93	0.002692958950694876
94-95	0.0038663799354804176
96-97	0.0031994716059979876
98-99	0.0030897271973489805
100	0.0015701892314396455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5922853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.22909518913981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72240135336905	46.36414120415683
2	14.73856797396149	18.048583628585913
3	4.478952828501535	8.227266872519627
4	1.8256710804771628	4.471367534823837
5	0.921061873138144	2.819789255273142
6	0.5677172358365205	2.0856487604129783
7	0.3632468721539529	1.5568894118590177
8	0.24763589133607625	1.2130017250291276
9	0.17871866422171181	0.9848503893336398
>10	0.8928200982750708	9.664822512680546
>50	0.04536189683883477	1.8684587357030893
>100	0.017154185535652768	1.7095902192214956
>500	3.312249844133729E-4	0.15462200817513322
>1k	2.760164388638501E-4	0.2670237413828655
>5k	2.760164382591504E-5	0.1254673577948592
>10k+	5.520328765183008E-5	0.4384766430479016
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13004	0.2195563523187221	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12941	0.21849267574258555	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7424	0.12534499843234334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6883755176770385E-5	0.0
4	0.0	0.0	0.0	5.065126553031115E-5	0.0
5	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
6	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
7	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
8	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
9	0.0	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
10-11	1.6883755176770385E-5	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
12-13	1.6883755176770385E-5	3.376751035354077E-5	0.0	5.065126553031115E-5	0.0
14-15	1.6883755176770385E-5	3.376751035354077E-5	0.0	7.597689829546673E-5	0.0
16-17	1.6883755176770385E-5	3.376751035354077E-5	0.0	1.097444086490075E-4	0.0
18-19	1.6883755176770385E-5	3.376751035354077E-5	0.0	1.266281638257779E-4	0.0
20-21	1.6883755176770385E-5	3.376751035354077E-5	0.0	2.1104693970962982E-4	0.0
22-23	1.6883755176770385E-5	3.376751035354077E-5	0.0	5.909314311869635E-4	0.0
24-25	1.6883755176770385E-5	5.065126553031115E-5	0.0	0.0010130253106062231	0.0
26-27	1.6883755176770385E-5	5.065126553031115E-5	0.0	0.0018740968246215126	0.0
28-29	1.6883755176770385E-5	5.065126553031115E-5	0.0	0.004786544592614404	0.0
30-31	1.6883755176770385E-5	5.065126553031115E-5	0.0	0.015330449700507509	0.0
32-33	1.6883755176770385E-5	5.065126553031115E-5	0.0	0.040318407362127676	0.0
34-35	1.6883755176770385E-5	5.065126553031115E-5	0.0	0.07813801895809333	0.0
36-37	1.6883755176770385E-5	5.909314311869635E-5	0.0	0.13497718076069085	0.0
38-39	1.6883755176770385E-5	8.441877588385192E-5	0.0	0.21177294118223094	0.0
40-41	1.6883755176770385E-5	9.286065347223711E-5	0.0	0.2892524936884302	0.0
42-43	1.6883755176770385E-5	1.013025310606223E-4	0.0	0.36625930104967996	0.0
44-45	1.6883755176770385E-5	1.013025310606223E-4	0.0	0.46102781885689215	0.0
46-47	1.6883755176770385E-5	1.013025310606223E-4	0.0	0.5751366782190948	0.0
48-49	1.6883755176770385E-5	1.3507004141416308E-4	0.0	0.6929261962098334	0.0
50-51	1.6883755176770385E-5	1.3507004141416308E-4	0.0	0.8094747581950793	0.0
52-53	1.6883755176770385E-5	1.3507004141416308E-4	0.0	0.9222666846534939	0.0
54-55	1.6883755176770385E-5	1.4351191900254827E-4	0.0	1.0467252859390568	0.0
56-57	1.6883755176770385E-5	2.279306948864002E-4	0.0	1.1931158851992443	0.0
58-59	1.6883755176770385E-5	2.8702383800509654E-4	0.0	1.3481509671099383	0.0
60-61	1.6883755176770385E-5	2.8702383800509654E-4	0.0	1.5066978700974007	0.0
62-63	1.6883755176770385E-5	2.8702383800509654E-4	0.0	1.663995375201782	0.0
64-65	1.6883755176770385E-5	2.8702383800509654E-4	0.0	1.8295996878531344	0.0
66-67	1.6883755176770385E-5	2.8702383800509654E-4	0.0	2.018537350158783	0.0
68-69	1.6883755176770385E-5	3.5455885871217807E-4	0.0	2.221480087383563	0.0
70-71	1.6883755176770385E-5	3.7988449147733364E-4	0.0	2.4399052281054416	0.0
72-73	1.6883755176770385E-5	4.052101242424892E-4	0.0	2.66125970035049	0.0
74-75	1.6883755176770385E-5	4.7274514494957074E-4	0.0	2.884454501909806	0.0
76-77	1.6883755176770385E-5	4.896289001263412E-4	0.0	3.1238577084388215	0.0
78-79	1.6883755176770385E-5	4.896289001263412E-4	0.0	3.3897768524729552	0.0
80-81	1.6883755176770385E-5	4.980707777147264E-4	0.0	3.6722842859682654	0.0
82-83	1.6883755176770385E-5	5.065126553031116E-4	0.0	3.964094668565977	0.0
84-85	1.6883755176770385E-5	5.065126553031116E-4	0.0	4.250341853832941	0.0
86-87	1.6883755176770385E-5	5.065126553031116E-4	0.0	4.55669759151544	0.0
88	1.6883755176770385E-5	5.402801656566523E-4	0.0	4.804323186815543	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9560	0.0	55.569984	1
GTATCAA	16575	0.0	37.78074	1
ATCAACG	20030	0.0	30.68943	3
TCAACGC	20165	0.0	30.530586	4
GTGGTAT	4290	0.0	29.588722	1
CAACGCA	20825	0.0	29.517855	5
TATCAAC	21055	0.0	29.463257	2
AACGCAG	21620	0.0	28.541555	6
TGGTATC	4440	0.0	26.038395	2
ACGCAGA	24850	0.0	24.661594	7
CGCAGAG	25225	0.0	24.015493	8
GTACATG	14770	0.0	21.962831	1
GCAGAGT	27825	0.0	21.4841	9
GAGTACT	19505	0.0	20.93447	12-13
TACATGG	14840	0.0	20.33121	2
CAGAGTA	27180	0.0	20.012577	10-11
ACATGGG	14980	0.0	19.57649	3
AGAGTAC	25590	0.0	19.32617	10-11
GTACTTT	21720	0.0	18.54321	14-15
AGTACTT	20825	0.0	17.778849	12-13
>>END_MODULE