FastQCFastQC Report
Wed 25 May 2016
SRR2049388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876042
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59800.046.4647371
GTATCAA104300.034.303431
TCAACGC111200.031.1575224
ATCAACG112600.030.7701283
CAACGCA117750.029.4243435
AACGCAG123800.028.020086
TATCAAC128400.027.2766762
GTACATG168300.025.281431
ACGCAGA142850.023.953767
TACATGG169400.023.7830522
CGCAGAG145100.023.3555668
ACATGGG177000.022.1775443
GTGGTAT39450.019.9024561
GCAGAGT164100.019.5058469
CATGGGG122950.018.9650234
TGGTATC36150.018.596362
AGAGTAC140750.017.9663610-11
CAGAGTA152900.017.84518410-11
GAGTACA127550.017.3242551
GAGTACT66800.015.83173812-13
Produced by FastQC (version 0.11.5)