##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049388_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5876042
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.900709695403812	31.0	31.0	34.0	28.0	34.0
2	31.095846830230283	31.0	31.0	34.0	28.0	34.0
3	31.160635849777794	31.0	31.0	34.0	28.0	34.0
4	34.84383893103555	37.0	35.0	37.0	32.0	37.0
5	34.867391860030956	37.0	35.0	37.0	32.0	37.0
6	34.97795012356957	37.0	35.0	37.0	32.0	37.0
7	34.95100988046035	37.0	35.0	37.0	32.0	37.0
8	34.904116240149406	37.0	35.0	37.0	32.0	37.0
9	36.54387647331316	39.0	37.0	39.0	32.0	39.0
10-11	36.502816181368345	39.0	37.0	39.0	32.0	39.0
12-13	36.44274649500463	39.0	37.0	39.0	32.0	39.0
14-15	37.68569923428049	40.0	38.0	41.0	32.0	41.0
16-17	37.653895002792694	40.0	38.0	41.0	32.0	41.0
18-19	37.68461823451909	40.0	38.0	41.0	32.0	41.0
20-21	37.758087246483264	40.0	38.0	41.0	32.0	41.0
22-23	37.71821916861724	40.0	38.0	41.0	32.0	41.0
24-25	37.728642936861235	40.0	38.0	41.0	32.0	41.0
26-27	37.71911569045967	40.0	38.0	41.0	32.0	41.0
28-29	37.668101419288696	40.0	38.0	41.0	32.0	41.0
30-31	37.608225060338235	40.0	38.0	41.0	32.0	41.0
32-33	37.55882948079677	40.0	38.0	41.0	32.0	41.0
34-35	37.46265224108337	40.0	38.0	41.0	32.0	41.0
36-37	37.386516723331795	40.0	38.0	41.0	32.0	41.0
38-39	37.28960812397189	40.0	37.0	41.0	31.0	41.0
40-41	37.18827273528678	40.0	37.0	41.0	31.0	41.0
42-43	37.05618535401891	40.0	37.0	41.0	31.0	41.0
44-45	36.93853745429321	40.0	37.0	41.0	31.0	41.0
46-47	36.82478809715792	40.0	37.0	41.0	30.0	41.0
48-49	36.697477060238846	40.0	36.0	41.0	30.0	41.0
50-51	36.55946511274085	40.0	36.0	41.0	30.0	41.0
52-53	36.41994866612594	40.0	36.0	41.0	30.0	41.0
54-55	36.28242173898689	39.0	35.0	41.0	30.0	41.0
56-57	36.155992418025605	39.0	35.0	41.0	30.0	41.0
58-59	35.952361810892434	39.0	35.0	41.0	29.0	41.0
60-61	35.73737934480387	39.0	35.0	41.0	29.0	41.0
62-63	35.52968477761051	38.5	35.0	40.0	28.5	41.0
64-65	35.28251670086769	38.0	35.0	40.0	28.0	41.0
66-67	35.01407112134324	37.5	34.0	40.0	28.0	41.0
68-69	34.72267063441684	37.0	34.0	40.0	28.0	41.0
70-71	34.43501578103084	36.5	34.0	39.5	28.0	41.0
72-73	34.086059970299736	36.0	34.0	39.0	27.5	41.0
74-75	33.73352113548542	36.0	34.0	39.0	26.5	40.5
76-77	33.39259658116807	35.0	34.0	38.5	26.0	40.0
78-79	33.07297497192838	35.0	33.0	37.0	26.0	39.0
80-81	32.716243689204404	35.0	33.0	37.0	26.0	39.0
82-83	32.400294024447064	35.0	33.0	36.0	26.0	39.0
84-85	32.10691278244778	35.0	33.0	36.0	25.0	37.0
86-87	31.79269455528058	35.0	33.0	36.0	25.0	37.0
88-89	31.54268255740854	35.0	33.0	35.0	24.0	37.0
90-91	31.339123852416304	35.0	33.0	35.0	23.5	36.0
92-93	31.102770623491118	35.0	32.0	35.0	23.0	36.0
94-95	30.905878480786896	35.0	32.0	35.0	20.0	36.0
96-97	30.660703582445464	35.0	32.0	35.0	18.5	35.5
98-99	30.459501906215102	35.0	32.0	35.0	8.5	35.0
100	30.345977785727197	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3036055889265121
1101	2	0.36543196820300494
1101	3	0.3221748560493154
1101	4	0.3841518703846063
1101	5	0.38088942212726096
1101	6	0.49523878157998524
1101	7	0.48297414964214624
1101	8	0.5178668256914989
1101	9	0.6169512112306421
1101	10-11	0.6021585674987762
1101	12-13	0.5639953710253351
1101	14-15	0.6598040836896573
1101	16-17	0.6647576310371193
1101	18-19	0.6243000414228845
1101	20-21	0.5941821996148136
1101	22-23	0.6284611362269956
1101	24-25	0.5861733988292102
1101	26-27	0.5328988528251131
1101	28-29	0.5777743175152565
1101	30-31	0.5925078036651925
1101	32-33	0.5732247207258041
1101	34-35	0.6149939407259808
1101	36-37	0.5733326970403425
1101	38-39	0.681498903565064
1101	40-41	0.7385881828836744
1101	42-43	0.6556684736949308
1101	44-45	0.5736633287027431
1101	46-47	0.5440946799204482
1101	48-49	0.4817037753823996
1101	50-51	0.5427860532617785
1101	52-53	0.5128310263241147
1101	54-55	0.5130899735647958
1101	56-57	0.5515303793818944
1101	58-59	0.596470406162041
1101	60-61	0.6477018818474605
1101	62-63	0.6051112537727477
1101	64-65	0.5838178799408595
1101	66-67	0.6074636376042193
1101	68-69	0.562788788335844
1101	70-71	0.6409489146155778
1101	72-73	0.7148471358849449
1101	74-75	0.6124072053089122
1101	76-77	0.6634883084556478
1101	78-79	0.619744257285987
1101	80-81	0.581537848158078
1101	82-83	0.6317994026871716
1101	84-85	0.596064103146233
1101	86-87	0.6235391162933226
1101	88-89	0.6507232485389345
1101	90-91	0.6183401272448812
1101	92-93	0.6248554535950426
1101	94-95	0.6245736777415694
1101	96-97	0.6424675773573725
1101	98-99	0.721034174554724
1101	100	0.7413620305830051
1102	1	0.09623720382015932
1102	2	0.15848451178915468
1102	3	0.19906720413710133
1102	4	0.288701644707416
1102	5	0.17786005675394279
1102	6	0.3483391727178997
1102	7	0.46672362642906506
1102	8	0.4007675170409897
1102	9	0.4990200525499233
1102	10-11	0.4719350665321187
1102	12-13	0.4768227923626611
1102	14-15	0.5105397535166105
1102	16-17	0.5472981331980122
1102	18-19	0.5603515854677852
1102	20-21	0.5044849331328365
1102	22-23	0.45857383975214105
1102	24-25	0.5619175662155556
1102	26-27	0.45725263809271155
1102	28-29	0.45367572584182625
1102	30-31	0.4226841264542074
1102	32-33	0.4645593975540834
1102	34-35	0.4829433643408976
1102	36-37	0.49444087190486385
1102	38-39	0.5144104918959869
1102	40-41	0.5288611307648452
1102	42-43	0.4794601041208395
1102	44-45	0.44529443849707917
1102	46-47	0.44248721756597575
1102	48-49	0.4832987542420142
1102	50-51	0.534527929659248
1102	52-53	0.5214059915474252
1102	54-55	0.5232335538610542
1102	56-57	0.4420332590889373
1102	58-59	0.5322940258987643
1102	60-61	0.42960925555521357
1102	62-63	0.4037077283086319
1102	64-65	0.42802409844764355
1102	66-67	0.5208581071076139
1102	68-69	0.560834156935826
1102	70-71	0.5428291741362159
1102	72-73	0.533447277164008
1102	74-75	0.5235396347060117
1102	76-77	0.5169241802905731
1102	78-79	0.5262087545244185
1102	80-81	0.519480714234561
1102	82-83	0.5261780613711693
1102	84-85	0.5483256270121046
1102	86-87	0.54615443355965
1102	88-89	0.5441088511027026
1102	90-91	0.5841046338995994
1102	92-93	0.5252868083790929
1102	94-95	0.5585609478722873
1102	96-97	0.6048902699746534
1102	98-99	0.6677221762366834
1102	100	0.5832096501031323
1103	1	0.015776842847024852
1103	2	0.14107211415475973
1103	3	0.16491477261059018
1103	4	0.18387946113644915
1103	5	0.20886274631004653
1103	6	0.28252782589420633
1103	7	0.2864387766935792
1103	8	0.2943373846390216
1103	9	0.4280494141911291
1103	10-11	0.3919804159047473
1103	12-13	0.37715671462467526
1103	14-15	0.4300610151598292
1103	16-17	0.40798724231981964
1103	18-19	0.40601660555266506
1103	20-21	0.3618540927710612
1103	22-23	0.38051481913752383
1103	24-25	0.41611055194674407
1103	26-27	0.3703018099755866
1103	28-29	0.41078851086482615
1103	30-31	0.4196201745027395
1103	32-33	0.4424586794072525
1103	34-35	0.44367531646070546
1103	36-37	0.4660048180267182
1103	38-39	0.46135775202673557
1103	40-41	0.4373769401764065
1103	42-43	0.4754408027246555
1103	44-45	0.3904767112971754
1103	46-47	0.47905033706675226
1103	48-49	0.48522614014255083
1103	50-51	0.39864876234369717
1103	52-53	0.47377314231511747
1103	54-55	0.4236238868891533
1103	56-57	0.41637110656939313
1103	58-59	0.5129403943117694
1103	60-61	0.4408157754348352
1103	62-63	0.44503525675814615
1103	64-65	0.45861773951748575
1103	66-67	0.5395553034629188
1103	68-69	0.520715100385047
1103	70-71	0.48853976782091735
1103	72-73	0.5174275153507892
1103	74-75	0.47584432115121444
1103	76-77	0.4834540596986727
1103	78-79	0.3595319889653297
1103	80-81	0.34149688052422533
1103	82-83	0.33756496278406445
1103	84-85	0.3848586044252187
1103	86-87	0.38027280769113503
1103	88-89	0.38921181509006786
1103	90-91	0.4110140530903337
1103	92-93	0.38648146892274227
1103	94-95	0.4127689302420201
1103	96-97	0.39512466555688164
1103	98-99	0.4434335337675108
1103	100	0.3992461018169706
1104	1	0.02516430857058083
1104	2	0.09508814536020083
1104	3	0.017888031888645628
1104	4	0.1708677537095582
1104	5	0.18181221122849678
1104	6	0.28183709087295483
1104	7	0.2893805021145113
1104	8	0.3029319225429248
1104	9	0.35787003355196134
1104	10-11	0.3742081289479344
1104	12-13	0.3289218040209576
1104	14-15	0.4547535774066418
1104	16-17	0.4512356136073379
1104	18-19	0.393347891642577
1104	20-21	0.40031976890199417
1104	22-23	0.3569108649467765
1104	24-25	0.4236328776232625
1104	26-27	0.36732197570644587
1104	28-29	0.3630091224733931
1104	30-31	0.35395444332847603
1104	32-33	0.33465119820025535
1104	34-35	0.3801241890468674
1104	36-37	0.33793057932488324
1104	38-39	0.47899452003212417
1104	40-41	0.4628086891312506
1104	42-43	0.38628173020044443
1104	44-45	0.33919446031342915
1104	46-47	0.28798401873022783
1104	48-49	0.33307274587837554
1104	50-51	0.36135656801872784
1104	52-53	0.3392552874104027
1104	54-55	0.3581593204545612
1104	56-57	0.31322632276692985
1104	58-59	0.3720306651630807
1104	60-61	0.36349417190779576
1104	62-63	0.35082414490105407
1104	64-65	0.3646739310349787
1104	66-67	0.4234554548931868
1104	68-69	0.35269758958447994
1104	70-71	0.3149508267115664
1104	72-73	0.35208718237302605
1104	74-75	0.36198039297944007
1104	76-77	0.345930319066305
1104	78-79	0.29218931575721285
1104	80-81	0.3354011515973383
1104	82-83	0.2731295990279605
1104	84-85	0.3086225408101768
1104	86-87	0.35193347162418576
1104	88-89	0.335988386841958
1104	90-91	0.35133863024593737
1104	92-93	0.35727536133355997
1104	94-95	0.3115229180657373
1104	96-97	0.31544148655535764
1104	98-99	0.29431558908973443
1104	100	0.2914968528736459
1105	1	-0.048719725415079296
1105	2	0.019685637065503414
1105	3	0.02088349177545723
1105	4	0.06964320253942446
1105	5	0.07289169923687666
1105	6	0.1575988067472025
1105	7	0.10712829972564464
1105	8	0.19685895582081514
1105	9	0.26840785581932636
1105	10-11	0.17196680164362022
1105	12-13	0.15469986486468912
1105	14-15	0.23408209408826508
1105	16-17	0.22204553275972216
1105	18-19	0.20462220118922403
1105	20-21	0.21073668664397616
1105	22-23	0.21645561665408763
1105	24-25	0.15328107757169107
1105	26-27	0.15542058718332896
1105	28-29	0.24947861595509124
1105	30-31	0.20166189666107925
1105	32-33	0.24482275980375334
1105	34-35	0.2547527389778068
1105	36-37	0.2277708996698493
1105	38-39	0.21080902160824877
1105	40-41	0.2370731681549998
1105	42-43	0.24866716099106867
1105	44-45	0.2058000108535225
1105	46-47	0.21897092748465496
1105	48-49	0.17625974346375983
1105	50-51	0.2381582327428049
1105	52-53	0.25705009188660455
1105	54-55	0.237920755477937
1105	56-57	0.22760018252271408
1105	58-59	0.22467734532494177
1105	60-61	0.24427227304730792
1105	62-63	0.15387455283469365
1105	64-65	0.24849438890833397
1105	66-67	0.2803518072410469
1105	68-69	0.20783010088068465
1105	70-71	0.17272791987373637
1105	72-73	0.17054127592695778
1105	74-75	0.2247831831748215
1105	76-77	0.27269284652906123
1105	78-79	0.17939105883533557
1105	80-81	0.18986636370708254
1105	82-83	0.17248636065460232
1105	84-85	0.19018248582342778
1105	86-87	0.2379019058309808
1105	88-89	0.19748936536628037
1105	90-91	0.1736106149390615
1105	92-93	0.170197754395101
1105	94-95	0.2104509009451263
1105	96-97	0.17620784280501667
1105	98-99	0.1888661529308706
1105	100	0.23964725872357562
1106	1	0.07427911664704467
1106	2	0.13264197884995
1106	3	0.1330487590012197
1106	4	0.14464100028713034
1106	5	0.12618741767541053
1106	6	0.2103945053973817
1106	7	0.25758935560667595
1106	8	0.20795826910109838
1106	9	0.29459346547297827
1106	10-11	0.25480736240727
1106	12-13	0.29114453598863577
1106	14-15	0.3724693177056011
1106	16-17	0.3850185861282682
1106	18-19	0.41240326922414283
1106	20-21	0.3501788082619228
1106	22-23	0.40020064562806823
1106	24-25	0.41348489123434007
1106	26-27	0.33748920425825446
1106	28-29	0.37593626440924055
1106	30-31	0.3649950406219915
1106	32-33	0.3772223339073122
1106	34-35	0.41846000411238293
1106	36-37	0.33012746282853556
1106	38-39	0.3968957086420204
1106	40-41	0.3965884109325657
1106	42-43	0.4281075335738578
1106	44-45	0.35123533549104735
1106	46-47	0.3634707353461195
1106	48-49	0.34492717655678007
1106	50-51	0.39660013605052313
1106	52-53	0.40083566145288074
1106	54-55	0.3923060589865983
1106	56-57	0.36147843180235384
1106	58-59	0.35133813080769016
1106	60-61	0.3911109749823467
1106	62-63	0.37214110579279236
1106	64-65	0.4766246511742338
1106	66-67	0.43855515236653986
1106	68-69	0.4400836177818519
1106	70-71	0.3801010414163528
1106	72-73	0.4314443525691729
1106	74-75	0.47288097339018975
1106	76-77	0.5187694032022634
1106	78-79	0.4053408359929378
1106	80-81	0.45126183204635595
1106	82-83	0.39311848366411084
1106	84-85	0.38481054775203916
1106	86-87	0.3403947277651582
1106	88-89	0.2817160237260943
1106	90-91	0.3583199176357681
1106	92-93	0.39299678514875325
1106	94-95	0.4522817513719808
1106	96-97	0.4226584249017904
1106	98-99	0.437200707609918
1106	100	0.41877977558516477
1107	1	0.0330609590243931
1107	2	0.020389512215462702
1107	3	0.022287634981669413
1107	4	0.04574669858384084
1107	5	0.09045217913118364
1107	6	0.1486340311649954
1107	7	0.1561247652656803
1107	8	0.21332402693876418
1107	9	0.2659353570395879
1107	10-11	0.2288066465189118
1107	12-13	0.25959931466812947
1107	14-15	0.30487934213810064
1107	16-17	0.35500989319822907
1107	18-19	0.30822826195924335
1107	20-21	0.22335454638147922
1107	22-23	0.23911062453804988
1107	24-25	0.2883203366877609
1107	26-27	0.2901606214802328
1107	28-29	0.33922769831865196
1107	30-31	0.3346970053239531
1107	32-33	0.33561142297055824
1107	34-35	0.3351183769505326
1107	36-37	0.28716489480978424
1107	38-39	0.3361401159201378
1107	40-41	0.4235972136155297
1107	42-43	0.4016539247606943
1107	44-45	0.3094818205914649
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2216	2	-0.7856050259483567
2216	3	-0.859924296653169
2216	4	-1.0584077376215006
2216	5	-1.023393824919026
2216	6	-2.485887476395874
2216	7	-2.343159187110899
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2216	9	-2.6451148896129055
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2216	12-13	-1.5688982375642482
2216	14-15	-1.8106600459671398
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2216	36-37	-2.1937582135178815
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2216	54-55	-2.3252720756253993
2216	56-57	-2.289720169827227
2216	58-59	-3.3154338348360213
2216	60-61	-4.038067349389486
2216	62-63	-3.11614325243211
2216	64-65	-3.1001662627432935
2216	66-67	-2.7235656798722374
2216	68-69	-3.094899057765396
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2216	74-75	-3.8817881384295703
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2216	80-81	-3.319986354317315
2216	82-83	-3.490851969442925
2216	84-85	-3.5089674354511153
2216	86-87	-3.181770152698334
2216	88-89	-3.171558558320097
2216	90-91	-3.33821874426917
2216	92-93	-3.3299800169323994
2216	94-95	-3.1766911679479435
2216	96-97	-3.4920655975634673
2216	98-99	-3.264770849473763
2216	100	-2.8811400974353525
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72600.0
3	12414.0
4	8282.0
5	7100.0
6	6395.0
7	6894.0
8	7477.0
9	8719.0
10	9936.0
11	11149.0
12	12464.0
13	14166.0
14	16214.0
15	17397.0
16	17573.0
17	18818.0
18	17708.0
19	15970.0
20	15854.0
21	17021.0
22	18701.0
23	22382.0
24	27449.0
25	34486.0
26	41970.0
27	49224.0
28	60627.0
29	77069.0
30	93539.0
31	112305.0
32	144805.0
33	194160.0
34	270310.0
35	395501.0
36	659303.0
37	1176378.0
38	1590455.0
39	591878.0
40	1349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.307050721208675	20.40690331344066	14.16825252006005	23.11779344529061
2	15.469204553909247	20.259819151868538	40.59683186326763	23.674144430954584
3	18.321914134846313	25.364479635620818	30.405850606850294	25.907755622682576
4	12.564033716804923	16.90393073844856	39.013425733828484	31.518609810918036
5	13.021341920750732	38.037819620986504	35.65297580200129	13.287862656261467
6	30.78921604516811	37.99350265699784	17.762996814326844	13.454284483507204
7	26.878718515209137	32.75637323540789	22.628952795288246	17.735955454094725
8	24.779775697943073	36.62441919660567	20.55857579941873	18.037229306032522
9	24.79959831979445	17.12069935125378	21.731304342052013	36.34839798689976
10-11	23.770709306214886	27.16211026671483	29.08361242849096	19.983567998579332
12-13	24.068234333027256	24.813420415440838	29.087975596396596	22.030369655135306
14-15	21.567754780838573	26.973808097672503	26.225409656035698	25.23302746545323
16-17	21.123460985527696	30.314992736948128	26.93696706122552	21.624579216298656
18-19	21.068472490902153	29.336499717538462	28.896190021190577	20.698837770368815
20-21	22.182359914274706	28.492482011096616	28.48636120824138	20.838796866387298
22-23	21.83986825892393	28.359124171106075	28.479898742435942	21.32110882753405
24-25	21.59405879909288	28.505676284910685	28.454336041514853	21.445928874481577
26-27	21.31617653443377	28.499028951991733	28.53667227883098	21.648122234743514
28-29	21.458240898731628	28.48703043931733	28.644031638938273	21.410697023012766
30-31	21.709780215308673	28.81131266918089	28.264213237681453	21.21469387782899
32-33	21.37855951859951	28.604393661426236	28.580643035844123	21.43640378413014
34-35	21.58559287434592	28.308074281634	28.609749353796637	21.49658349022344
36-37	21.774499999489247	28.617272197019457	28.153228615586716	21.45499918790458
38-39	21.6127736151333	28.764120217853574	28.283100684921013	21.340005482092117
40-41	21.525151150706687	28.469707205520013	28.49625817725664	21.50888346651666
42-43	21.42508367091698	28.498830190516696	28.415303988725153	21.66078214984117
44-45	21.55165860599377	28.553424533927398	28.330957623262748	21.563959236816086
46-47	21.583164212229345	28.62347977216257	28.18262848968959	21.610727525918495
48-49	21.52346112874989	28.53239768821791	28.217121399885624	21.727019783146574
50-51	21.552179487299536	28.44489644325045	28.189978877748107	21.812945191701907
52-53	21.515133277496183	28.449705322697888	28.276379754014062	21.758781645791863
54-55	21.592612666464124	28.73103902318063	28.070873169619258	21.605475140735983
56-57	21.550268740249596	28.505559736555302	28.124553897825333	21.819617625369766
58-59	21.437668222434674	28.196231579114034	28.36293985758802	22.003160340863268
60-61	21.661268798511756	28.514678691426347	28.083115961564374	21.74093654849752
62-63	21.43235278569886	28.558296275040362	28.11243055214466	21.89692038711612
64-65	21.517137072747865	28.34699993122154	28.203092919700122	21.93277007633047
66-67	21.443707470543274	28.378292335376848	28.090917912721935	22.087082281357947
68-69	21.742733750239942	28.52523933540666	27.98224493695965	21.74978197739375
70-71	21.717880661659052	28.35880838718904	28.040296439053957	21.883014512097958
72-73	21.566154576106943	28.477491682099977	28.124244277062616	21.83210946473046
74-75	21.637532133676093	28.474880403139313	28.04516590340319	21.842421559781407
76-77	21.691382799648444	28.49231659746887	27.912280888427333	21.90401971445535
78-79	21.678689494717755	28.584605672691023	27.99423237937249	21.742472453218735
80-81	21.69161892513846	28.486961715014274	27.957544472359285	21.863874887487984
82-83	21.60100410161379	28.478325543941573	28.124191604992077	21.796478749452557
84-85	21.679352742423447	28.350512787358362	27.979740981542108	21.990393488676084
86-87	21.849417193382568	28.458968255400492	27.882333092377053	21.809281458839887
88-89	21.677048268382883	28.347395136600884	27.977147565725435	21.998409029290798
90-91	21.702528758879975	28.264255668992437	27.991950969661467	22.041264602466125
92-93	21.832176199226026	28.407334472733474	27.829306531609333	21.931182796431166
94-95	21.73688907593527	28.205498823071945	27.96715939994886	22.09045270104393
96-97	21.83612710732837	28.222687993040214	27.910777356594796	22.030407543036624
98-99	21.804992203935914	28.301559110707515	27.83057881478723	22.062869870569337
100	21.855357739103976	28.177708736595143	27.84210868472349	22.12482483957739
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2222.0
1	1753.5
2	1543.0
3	1890.0
4	1842.5
5	1561.0
6	1277.0
7	1149.5
8	1193.5
9	1178.5
10	1021.0
11	923.5
12	978.0
13	1071.5
14	1188.0
15	1412.5
16	1635.0
17	1925.0
18	2627.0
19	3605.5
20	4985.0
21	7187.0
22	10637.5
23	15554.0
24	22232.5
25	30994.0
26	42488.5
27	56813.0
28	73292.5
29	91055.0
30	108558.5
31	124394.5
32	137694.5
33	149952.5
34	162181.0
35	173379.5
36	183348.5
37	193893.0
38	205054.5
39	211026.5
40	214281.5
41	217223.5
42	216337.5
43	213895.0
44	209575.5
45	204370.5
46	201259.5
47	195708.5
48	188111.5
49	183439.0
50	179205.5
51	171182.0
52	159230.5
53	148538.5
54	140881.5
55	133827.5
56	123921.5
57	111521.5
58	100506.5
59	90756.5
60	79652.5
61	68128.0
62	58297.0
63	48842.0
64	39992.5
65	32501.0
66	26141.0
67	20741.0
68	16017.0
69	12293.5
70	9174.5
71	6731.5
72	5116.5
73	3689.5
74	2701.5
75	2010.5
76	1343.5
77	911.5
78	650.0
79	441.5
80	295.0
81	189.0
82	144.5
83	111.0
84	79.5
85	65.0
86	59.5
87	50.5
88	39.0
89	27.5
90	24.0
91	23.5
92	22.0
93	17.5
94	13.0
95	14.0
96	14.0
97	16.0
98	15.5
99	13.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02011558120244886
2	0.042460554230211424
3	0.044247471342104085
4	0.047123556979340855
5	0.05025491648970515
6	0.048876437574816516
7	0.04645984490920929
8	0.046153516261456265
9	0.04617053451966477
10-11	0.04674064616964957
12-13	0.047838323824097924
14-15	0.04594929716295425
16-17	0.042171243840666894
18-19	0.045540858965950205
20-21	0.044749509959254884
22-23	0.04600035193757975
24-25	0.0422393168735009
26-27	0.042392481197377416
28-29	0.04288601068542396
30-31	0.04290302894363246
32-33	0.042758373748860205
34-35	0.04261371855408794
36-37	0.039958870273561695
38-39	0.04036730847056573
40-41	0.04142244047949283
42-43	0.04145647699590983
44-45	0.04015458024295946
46-47	0.03901435694298985
48-49	0.03922708517059612
50-51	0.04047792714892099
52-53	0.041073566186218546
54-55	0.04027370805041897
56-57	0.03824853532360728
58-59	0.03928664907432588
60-61	0.038673991778819826
62-63	0.03915050300865787
64-65	0.035908524819938316
66-67	0.03696365682886542
68-69	0.03785711538481175
70-71	0.03951639556014065
72-73	0.03596808872366807
74-75	0.036453109082610374
76-77	0.037091293765429176
78-79	0.03771246019003949
80-81	0.037865624513916
82-83	0.0363254721460466
84-85	0.03766991454451823
86-87	0.037848606255707504
88-89	0.03862293700419432
90-91	0.038988829555677104
92-93	0.03728700373482695
94-95	0.03873355568254958
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5876042.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.730459885864526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02616078603603	46.17103707892649
2	14.08984951653918	17.11366081724101
3	4.484703778003558	8.170743686700908
4	1.8950469069256164	4.603482806515113
5	1.0029707861086288	3.0455438546232045
6	0.5911858731576308	2.1541793972933574
7	0.3826612640769465	1.626743618352945
8	0.2782349433235746	1.3517868851486514
9	0.19833480022552727	1.0840467266160605
>10	0.9801876556214503	10.482592649078502
>50	0.05180084180863877	2.1487807342135694
>100	0.01849858851526556	1.85850144158619
>500	2.8020181491592006E-4	0.1012326445666741
>1k	8.405784291001594E-5	0.0876676591373204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7018258208501572E-5	0.0
4	0.0	0.0	0.0	1.7018258208501572E-5	0.0
5	0.0	0.0	0.0	1.7018258208501572E-5	0.0
6	0.0	0.0	0.0	1.7018258208501572E-5	0.0
7	0.0	0.0	0.0	1.7018258208501572E-5	0.0
8	0.0	0.0	0.0	1.7018258208501572E-5	0.0
9	0.0	0.0	0.0	3.4036516417003145E-5	0.0
10-11	0.0	0.0	0.0	3.4036516417003145E-5	0.0
12-13	0.0	0.0	0.0	4.254564552125393E-5	0.0
14-15	0.0	0.0	0.0	5.9563903729755505E-5	0.0
16-17	0.0	0.0	0.0	7.658216193825708E-5	0.0
18-19	0.0	1.7018258208501572E-5	0.0	8.509129104250786E-5	0.0
20-21	0.0	1.7018258208501572E-5	0.0	1.0210954925100944E-4	8.509129104250786E-6
22-23	0.0	1.7018258208501572E-5	0.0	2.38255614919022E-4	1.7018258208501572E-5
24-25	0.0	1.7018258208501572E-5	0.0	5.95639037297555E-4	1.7018258208501572E-5
26-27	0.0	1.7018258208501572E-5	0.0	0.0012082963328036117	1.7018258208501572E-5
28-29	0.0	2.552738731275236E-5	0.0	0.00476511229838044	1.7018258208501572E-5
30-31	0.0	3.4036516417003145E-5	0.0	0.015512142357049185	1.7018258208501572E-5
32-33	0.0	3.4036516417003145E-5	0.0	0.03994185201535319	1.7018258208501572E-5
34-35	0.0	3.4036516417003145E-5	0.0	0.07892217244192604	1.7018258208501572E-5
36-37	0.0	3.4036516417003145E-5	0.0	0.1397028816335894	1.7018258208501572E-5
38-39	0.0	3.4036516417003145E-5	0.0	0.22135648451797996	1.7018258208501572E-5
40-41	0.0	3.4036516417003145E-5	0.0	0.30411627418592313	1.7018258208501572E-5
42-43	0.0	3.4036516417003145E-5	0.0	0.3858464592322519	1.7018258208501572E-5
44-45	0.0	3.4036516417003145E-5	0.0	0.4851990506534841	1.7018258208501572E-5
46-47	0.0	3.4036516417003145E-5	0.0	0.6041907120473271	1.7018258208501572E-5
48-49	0.0	3.4036516417003145E-5	0.0	0.7261350412403451	1.7018258208501572E-5
50-51	0.0	3.4036516417003145E-5	0.0	0.8477390052691931	1.7018258208501572E-5
52-53	0.0	3.4036516417003145E-5	0.0	0.9665519749518469	1.7018258208501572E-5
54-55	0.0	3.4036516417003145E-5	0.0	1.0967586685050925	1.7018258208501572E-5
56-57	0.0	5.105477462550472E-5	0.0	1.2488338238562624	1.7018258208501572E-5
58-59	0.0	8.509129104250786E-5	0.0	1.4123707761108584	1.7018258208501572E-5
60-61	0.0	1.0210954925100944E-4	0.0	1.5808940780205452	1.7018258208501572E-5
62-63	0.0	1.0210954925100944E-4	0.0	1.7435630991065074	1.7018258208501572E-5
64-65	0.0	1.0210954925100944E-4	0.0	1.9190809051398885	1.7018258208501572E-5
66-67	0.0	1.0210954925100944E-4	0.0	2.1135145051720188	1.7018258208501572E-5
68-69	0.0	1.1061867835526022E-4	0.0	2.3280466681483896	1.7018258208501572E-5
70-71	0.0	1.3614606566801258E-4	0.0	2.553521571152827	1.7018258208501572E-5
72-73	0.0	1.3614606566801258E-4	0.0	2.7814556124683927	1.7018258208501572E-5
74-75	0.0	1.3614606566801258E-4	0.0	3.013703782239814	1.7018258208501572E-5
76-77	0.0	1.6167345298076494E-4	0.0	3.2624256259570643	1.7018258208501572E-5
78-79	0.0	1.7018258208501572E-4	0.0	3.535483578912472	1.7018258208501572E-5
80-81	0.0	1.7018258208501572E-4	0.0	3.8258916461114474	1.7018258208501572E-5
82-83	0.0	1.7018258208501572E-4	0.0	4.122290140199814	1.7018258208501572E-5
84-85	0.0	1.7018258208501572E-4	0.0	4.41984587584636	1.7018258208501572E-5
86-87	0.0	1.7018258208501572E-4	0.0	4.738648906866221	1.7018258208501572E-5
88	0.0	1.7018258208501572E-4	0.0	4.995233185875799	1.7018258208501572E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5980	0.0	46.464737	1
GTATCAA	10430	0.0	34.30343	1
TCAACGC	11120	0.0	31.157522	4
ATCAACG	11260	0.0	30.770128	3
CAACGCA	11775	0.0	29.424343	5
AACGCAG	12380	0.0	28.02008	6
TATCAAC	12840	0.0	27.276676	2
GTACATG	16830	0.0	25.28143	1
ACGCAGA	14285	0.0	23.95376	7
TACATGG	16940	0.0	23.783052	2
CGCAGAG	14510	0.0	23.355566	8
ACATGGG	17700	0.0	22.177544	3
GTGGTAT	3945	0.0	19.902456	1
GCAGAGT	16410	0.0	19.505846	9
CATGGGG	12295	0.0	18.965023	4
TGGTATC	3615	0.0	18.59636	2
AGAGTAC	14075	0.0	17.96636	10-11
CAGAGTA	15290	0.0	17.845184	10-11
GAGTACA	12755	0.0	17.324255	1
GAGTACT	6680	0.0	15.831738	12-13
>>END_MODULE