FastQCFastQC Report
Wed 25 May 2016
SRR2049388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876042
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156700.26667610612721965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147020.25020243218139016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87250.14848430286917624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97350.061.0468641
GTATCAA177250.040.239411
ATCAACG219550.032.04443
TCAACGC220400.032.0061074
CAACGCA229850.030.7515545
TATCAAC230100.030.7385652
AACGCAG238700.029.6980236
GTGGTAT41050.026.8012451
ACGCAGA275200.025.6055347
CGCAGAG277850.025.225888
GTACATG165450.025.0926281
TGGTATC42400.023.9415172
TACATGG169350.023.0888252
GCAGAGT302750.022.778059
ACATGGG171950.021.8924353
GAGTACT217350.021.24305712-13
CAGAGTA297700.020.8653810-11
AGAGTAC284950.019.80976310-11
GTACTTT235800.019.26274114-15
CATGGGG119650.018.5785664
Produced by FastQC (version 0.11.5)