##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049388_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5876042 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.873773366493978 31.0 31.0 34.0 30.0 34.0 2 32.09006215408263 33.0 31.0 34.0 30.0 34.0 3 32.055793508623665 34.0 31.0 34.0 30.0 34.0 4 35.75014184718217 37.0 35.0 37.0 33.0 37.0 5 35.830182970101305 37.0 35.0 37.0 35.0 37.0 6 35.86753600467798 37.0 35.0 37.0 35.0 37.0 7 35.814675933221714 37.0 35.0 37.0 35.0 37.0 8 35.81383182761457 37.0 35.0 37.0 35.0 37.0 9 37.565146062604725 39.0 37.0 39.0 35.0 39.0 10-11 37.5266250649672 39.0 37.0 39.0 35.0 39.0 12-13 37.47113737444354 39.0 37.0 39.0 35.0 39.0 14-15 38.76918178937455 40.0 38.0 41.0 35.0 41.0 16-17 38.79813018695238 40.0 38.0 41.0 35.0 41.0 18-19 38.87031840480378 40.0 38.0 41.0 35.0 41.0 20-21 38.89774196644612 40.0 38.0 41.0 35.0 41.0 22-23 38.86610604553201 40.0 38.0 41.0 35.0 41.0 24-25 38.845208134999716 40.0 38.0 41.0 35.0 41.0 26-27 38.73065900481991 40.0 38.0 41.0 34.5 41.0 28-29 38.65024135293791 40.0 38.0 41.0 34.0 41.0 30-31 38.545906921700016 40.0 38.0 41.0 34.0 41.0 32-33 38.40785498129523 40.0 38.0 41.0 34.0 41.0 34-35 38.306493895040234 40.0 38.0 41.0 34.0 41.0 36-37 38.195360584556745 40.0 38.0 41.0 34.0 41.0 38-39 38.1028164026057 40.0 38.0 41.0 33.5 41.0 40-41 37.989141500350065 40.0 38.0 41.0 33.0 41.0 42-43 37.853542401500874 40.0 37.5 41.0 33.0 41.0 44-45 37.67597253389271 40.0 37.0 41.0 33.0 41.0 46-47 37.44282971428727 40.0 37.0 41.0 33.0 41.0 48-49 37.231809524166096 40.0 37.0 41.0 32.0 41.0 50-51 37.08297983574658 40.0 37.0 41.0 31.5 41.0 52-53 36.945066253781036 40.0 36.0 41.0 31.0 41.0 54-55 36.802285449967854 40.0 36.0 41.0 31.0 41.0 56-57 36.657853619834576 39.0 36.0 41.0 31.0 41.0 58-59 36.4664992183514 39.0 35.0 41.0 31.0 41.0 60-61 36.28774351851127 39.0 35.0 41.0 31.0 41.0 62-63 36.08166900440807 39.0 35.0 41.0 30.0 41.0 64-65 35.855505457585224 38.0 35.0 40.5 30.0 41.0 66-67 35.5827420906794 38.0 35.0 40.0 30.0 41.0 68-69 35.292801685216 37.0 35.0 40.0 29.0 41.0 70-71 34.96051806641273 37.0 34.5 39.5 29.0 41.0 72-73 34.65901477559214 36.0 34.0 39.0 29.0 41.0 74-75 34.35194889689352 36.0 34.0 39.0 29.0 41.0 76-77 34.0255820159216 35.0 34.0 38.5 29.0 40.0 78-79 33.69021119998802 35.0 34.0 37.0 28.5 39.0 80-81 33.37314386112284 35.0 34.0 37.0 28.0 39.0 82-83 33.07445130582797 35.0 34.0 36.5 28.0 39.0 84-85 32.80301859653147 35.0 34.0 36.0 27.0 37.0 86-87 32.5729728446461 35.0 33.0 36.0 27.0 37.0 88-89 32.348030698214885 35.0 33.0 35.0 27.0 37.0 90-91 32.157837027032826 35.0 33.0 35.0 26.5 36.0 92-93 31.978838902104513 35.0 33.0 35.0 26.0 36.0 94-95 31.825935468126332 35.0 33.0 35.0 26.0 36.0 96-97 31.626266456230233 35.0 33.0 35.0 25.5 36.0 98-99 31.483733778621733 35.0 33.0 35.0 25.0 35.0 100 31.374240687864383 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1709488924903937 1101 2 0.19324058174535708 1101 3 0.3170602502989084 1101 4 0.17020316054364315 1101 5 0.13413415652608762 1101 6 0.14468934229593344 1101 7 0.028728170458983016 1101 8 0.08908945837347204 1101 9 0.21584237346496593 1101 10-11 0.21555094294484434 1101 12-13 0.20046775197732103 1101 14-15 0.27313763119927614 1101 16-17 0.18091729969709291 1101 18-19 0.28079421763606405 1101 20-21 0.24117237006836945 1101 22-23 0.23918844081086377 1101 24-25 0.2282944037042398 1101 26-27 0.29648653172390027 1101 28-29 0.3171173311879727 1101 30-31 0.2584809157248813 1101 32-33 0.2562084088590382 1101 34-35 0.22736039446150613 1101 36-37 0.26734325780370227 1101 38-39 0.28937762164724745 1101 40-41 0.26340799774301615 1101 42-43 0.2766549752608043 1101 44-45 0.2929762959018092 1101 46-47 0.2635894685505491 1101 48-49 0.2761118792772024 1101 50-51 0.20201999396976333 1101 52-53 0.2289069860488624 1101 54-55 0.23761569939177463 1101 56-57 0.19735898253873074 1101 58-59 0.256442777971138 1101 60-61 0.30153132024634743 1101 62-63 0.2201044933364642 1101 64-65 0.1720908981207856 1101 66-67 0.1584912461233685 1101 68-69 0.1473118915160896 1101 70-71 0.20327907322000982 1101 72-73 0.10556807906750976 1101 74-75 0.1446012178743672 1101 76-77 0.07358358741370097 1101 78-79 0.1612200697059194 1101 80-81 0.2190296967516332 1101 82-83 0.18148619584859205 1101 84-85 0.19341936967751394 1101 86-87 0.11458920263718397 1101 88-89 0.15040089007125346 1101 90-91 0.2024553670415088 1101 92-93 0.1779611754997532 1101 94-95 0.2582420544781634 1101 96-97 0.24613703365079687 1101 98-99 0.2308832240425538 1101 100 0.22802282585158196 1102 1 0.23069128896797508 1102 2 0.21100626684924606 1102 3 0.33226937370496756 1102 4 0.16791581483018092 1102 5 0.11182106590980823 1102 6 0.15771042257718904 1102 7 0.13297147483190486 1102 8 0.15016869837745617 1102 9 0.12606495744349644 1102 10-11 0.21402481377925398 1102 12-13 0.17280161843660125 1102 14-15 0.21893201540130747 1102 16-17 0.17881614066853757 1102 18-19 0.24163853725362117 1102 20-21 0.18127705909306258 1102 22-23 0.2129019314824916 1102 24-25 0.24415228185144855 1102 26-27 0.33190636225282333 1102 28-29 0.3342867774881384 1102 30-31 0.29364912628300743 1102 32-33 0.29321922739913475 1102 34-35 0.2813751222341665 1102 36-37 0.32535403421313447 1102 38-39 0.35577679111541016 1102 40-41 0.3485777676042332 1102 42-43 0.37019806668115507 1102 44-45 0.3870361492862031 1102 46-47 0.3267390745593275 1102 48-49 0.2646982643737914 1102 50-51 0.28837863606966607 1102 52-53 0.3590995579401053 1102 54-55 0.3299114314801912 1102 56-57 0.3512032572441228 1102 58-59 0.27552738557557177 1102 60-61 0.35931532878664285 1102 62-63 0.33820069778715833 1102 64-65 0.2991563908310084 1102 66-67 0.2784215024188299 1102 68-69 0.3219122666225829 1102 70-71 0.3339931386493973 1102 72-73 0.37230907982143435 1102 74-75 0.31422353108288803 1102 76-77 0.30731843486888266 1102 78-79 0.36703128319489053 1102 80-81 0.3508136950427101 1102 82-83 0.40287527806940204 1102 84-85 0.3427766306868776 1102 86-87 0.3305593627005905 1102 88-89 0.3371456890849416 1102 90-91 0.35675095530820755 1102 92-93 0.3626530987042784 1102 94-95 0.33649806489621525 1102 96-97 0.3825503707820843 1102 98-99 0.4313274417544015 1102 100 0.47025728859048854 1103 1 0.19945148493040676 1103 2 0.22128360193964625 1103 3 0.3764710989512068 1103 4 0.22239510632887516 1103 5 0.18035610858253648 1103 6 0.1671135605530054 1103 7 0.1864749879389649 1103 8 0.18732922732305468 1103 9 0.22031215587853836 1103 10-11 0.22958990149492564 1103 12-13 0.22261277276009395 1103 14-15 0.28579324972781706 1103 16-17 0.2814354365081684 1103 18-19 0.2957244920622202 1103 20-21 0.34134807265532885 1103 22-23 0.28526931601281547 1103 24-25 0.31621673572798414 1103 26-27 0.33643710970335405 1103 28-29 0.3238994670484274 1103 30-31 0.30502876487297925 1103 32-33 0.3128942537093522 1103 34-35 0.33444072771672495 1103 36-37 0.3576592063876234 1103 38-39 0.3729526882925569 1103 40-41 0.41030256654417485 1103 42-43 0.41849138203354386 1103 44-45 0.44793471723869516 1103 46-47 0.4161827687624964 1103 48-49 0.3180680068871453 1103 50-51 0.3951711005506411 1103 52-53 0.372438359989836 1103 54-55 0.34577648086196433 1103 56-57 0.3381594179809895 1103 58-59 0.3941053369830243 1103 60-61 0.4262872030533771 1103 62-63 0.4200089069326438 1103 64-65 0.4320705796751838 1103 66-67 0.4114556715951565 1103 68-69 0.46444734810165045 1103 70-71 0.4487076785738324 1103 72-73 0.4510082604140564 1103 74-75 0.40428400900091077 1103 76-77 0.4133154129161909 1103 78-79 0.3641886761382125 1103 80-81 0.3816720166694907 1103 82-83 0.38985517626014143 1103 84-85 0.35471297975175986 1103 86-87 0.35508236304367813 1103 88-89 0.36674912580594565 1103 90-91 0.38451296951915737 1103 92-93 0.39115268602943587 1103 94-95 0.40119680706110117 1103 96-97 0.44486104273536853 1103 98-99 0.4513910153658145 1103 100 0.47216940334753943 1104 1 0.10022363822166369 1104 2 0.11566907323072684 1104 3 0.27448040933848716 1104 4 0.18679029405736713 1104 5 0.14867859012633744 1104 6 0.12293685355843564 1104 7 0.13918929308152173 1104 8 0.1714723507736764 1104 9 0.1379433165382764 1104 10-11 0.17235812350794077 1104 12-13 0.1486132751027398 1104 14-15 0.1894357350877769 1104 16-17 0.17999947706009323 1104 18-19 0.21452675359260098 1104 20-21 0.212761398363007 1104 22-23 0.19067001451676902 1104 24-25 0.18078317170908775 1104 26-27 0.21908672865096435 1104 28-29 0.22071279127894883 1104 30-31 0.2286913761473457 1104 32-33 0.2625395540429025 1104 34-35 0.23996258320364916 1104 36-37 0.26058531481643854 1104 38-39 0.2556516543807703 1104 40-41 0.28182366601818387 1104 42-43 0.318098223667171 1104 44-45 0.2523824668563748 1104 46-47 0.27580232223064627 1104 48-49 0.29047080766674327 1104 50-51 0.3024482051600259 1104 52-53 0.20239560338928442 1104 54-55 0.22406787058351796 1104 56-57 0.22051264171743412 1104 58-59 0.29169168774580356 1104 60-61 0.2781282338742628 1104 62-63 0.2440678793825981 1104 64-65 0.16234756394668182 1104 66-67 0.21999497179952954 1104 68-69 0.22719330705049146 1104 70-71 0.22258144202153574 1104 72-73 0.2193106560264937 1104 74-75 0.2707713947083761 1104 76-77 0.21035945325043315 1104 78-79 0.22348501242990437 1104 80-81 0.23956737729691469 1104 82-83 0.27381705847128046 1104 84-85 0.28796197938407886 1104 86-87 0.26282578390159017 1104 88-89 0.2873223797862394 1104 90-91 0.2331489004348839 1104 92-93 0.32175219749205297 1104 94-95 0.3478475571404154 1104 96-97 0.41878620523177545 1104 98-99 0.3890256353926915 1104 100 0.3717428008225774 1105 1 0.20748024000666732 1105 2 0.17046000634437064 1105 3 0.2777772656379014 1105 4 0.1590595309504934 1105 5 0.18656721295124612 1105 6 0.1164269269884528 1105 7 0.1511955619360279 1105 8 0.1866271769099157 1105 9 0.19125538183777735 1105 10-11 0.2358743952822948 1105 12-13 0.18687696666241038 1105 14-15 0.2531031822551242 1105 16-17 0.24630286371232302 1105 18-19 0.21968602975977802 1105 20-21 0.23477062020778305 1105 22-23 0.2220699522067946 1105 24-25 0.22656767669391087 1105 26-27 0.20550885207551062 1105 28-29 0.22877953278263163 1105 30-31 0.1921788868197538 1105 32-33 0.2391123520795162 1105 34-35 0.25727969464630007 1105 36-37 0.22710167518846447 1105 38-39 0.2550506907711849 1105 40-41 0.27786212180389214 1105 42-43 0.27408913813872005 1105 44-45 0.28000429221951606 1105 46-47 0.26256837133114175 1105 48-49 0.2694347152806458 1105 50-51 0.22990066503883355 1105 52-53 0.25361351503690344 1105 54-55 0.2358566085853866 1105 56-57 0.19306709044141712 1105 58-59 0.17860626385318312 1105 60-61 0.20047677124139085 1105 62-63 0.2677254034528218 1105 64-65 0.24587257168373355 1105 66-67 0.28812040965144803 1105 68-69 0.23497962847294218 1105 70-71 0.2028988499031712 1105 72-73 0.22468579289291313 1105 74-75 0.23670009967726457 1105 76-77 0.2126917495687195 1105 78-79 0.1512391669629949 1105 80-81 0.16984822207429318 1105 82-83 0.12295359314051524 1105 84-85 0.1445022311679196 1105 86-87 0.12788843082537937 1105 88-89 0.18495346204087326 1105 90-91 0.17909815014630937 1105 92-93 0.21397466690876144 1105 94-95 0.18198470260860589 1105 96-97 0.26211393975140496 1105 98-99 0.25653975600947376 1105 100 0.31504496343793775 1106 1 0.11980198842902468 1106 2 0.1468218727819206 1106 3 0.2684621214191054 1106 4 0.15230760413931677 1106 5 0.10862469109788009 1106 6 0.08120416757270732 1106 7 0.14817601231896305 1106 8 0.1309561155264376 1106 9 0.12927123550098685 1106 10-11 0.1057579567774809 1106 12-13 0.14312844600145525 1106 14-15 0.2071728934345174 1106 16-17 0.18097702612237043 1106 18-19 0.1755779419403396 1106 20-21 0.22120928460878986 1106 22-23 0.23020589564529814 1106 24-25 0.1675045850380812 1106 26-27 0.1947002233318429 1106 28-29 0.1958437900363421 1106 30-31 0.25183419284346087 1106 32-33 0.20316662834281374 1106 34-35 0.22702052465923828 1106 36-37 0.24994738730748622 1106 38-39 0.22996703710056465 1106 40-41 0.23307757867868162 1106 42-43 0.2591277318123204 1106 44-45 0.2515298083444719 1106 46-47 0.3065879477582314 1106 48-49 0.29811028713216103 1106 50-51 0.27656870199582073 1106 52-53 0.3050722039754632 1106 54-55 0.29237162411605055 1106 56-57 0.23447204442392433 1106 58-59 0.3278190320651717 1106 60-61 0.2893393366546704 1106 62-63 0.2921515539541062 1106 64-65 0.23423190648864534 1106 66-67 0.26324314851284925 1106 68-69 0.27713859534213015 1106 70-71 0.28282343712448466 1106 72-73 0.3135199971266047 1106 74-75 0.2370649713912769 1106 76-77 0.1858138986094957 1106 78-79 0.29152823651459414 1106 80-81 0.29198740697284364 1106 82-83 0.2990953620150947 1106 84-85 0.2413566744193929 1106 86-87 0.2676585029188203 1106 88-89 0.3295311913336363 1106 90-91 0.3216009811575944 1106 92-93 0.3510557570300321 1106 94-95 0.31653557719002023 1106 96-97 0.42209113846094937 1106 98-99 0.4339465432872238 1106 100 0.4454412284180407 1107 1 0.14835981013291644 1107 2 0.12344875401554134 1107 3 0.23245200170111957 1107 4 0.19009676878241066 1107 5 0.15474920328981057 1107 6 0.14440862852997327 1107 7 0.09918832085936202 1107 8 0.1192509293268742 1107 9 0.15096676843247536 1107 10-11 0.11512808103524463 1107 12-13 0.14853454427272794 1107 14-15 0.13717012466770484 1107 16-17 0.1502993267929824 1107 18-19 0.13808537208822003 1107 20-21 0.165692398249206 1107 22-23 0.13852023424053073 1107 24-25 0.14285162251995587 1107 26-27 0.26446007646248404 1107 28-29 0.23186999229849192 1107 30-31 0.20819979353490936 1107 32-33 0.21514280447628664 1107 34-35 0.14334898396522533 1107 36-37 0.16458000188391964 1107 38-39 0.18464874740026005 1107 40-41 0.1607568407399711 1107 42-43 0.23737663022975397 1107 44-45 0.1498028835838312 1107 46-47 0.19507530309951449 1107 48-49 0.2805175926855341 1107 50-51 0.27507139780188794 1107 52-53 0.2809075733501629 1107 54-55 0.24756834998693478 1107 56-57 0.2913990063508862 1107 58-59 0.311686625146244 1107 60-61 0.20062015321638427 1107 62-63 0.21169690872417846 1107 64-65 0.20627053577446475 1107 66-67 0.23455244470977732 1107 68-69 0.19496933513916304 1107 70-71 0.1561037772587568 1107 72-73 0.2244995356904127 1107 74-75 0.22598965469799026 1107 76-77 0.20143950271825162 1107 78-79 0.23355878399458163 1107 80-81 0.27568969719198577 1107 82-83 0.2587864948522878 1107 84-85 0.21758621267140654 1107 86-87 0.19707273413079918 1107 88-89 0.21725482225951254 1107 90-91 0.2824440082927566 1107 92-93 0.3168689825061719 1107 94-95 0.39446703399788063 1107 96-97 0.3772247439316345 1107 98-99 0.4378314661047291 1107 100 0.44346487767764486 1108 1 0.10140289264470326 1108 2 0.09651849828757264 1108 3 0.18700107647899245 1108 4 0.06953851160552205 1108 5 0.10089179314596208 1108 6 0.03618446046640855 1108 7 0.011412006925311857 1108 8 -0.00844310508809798 1108 9 0.061607698653205034 1108 10-11 0.07360432044867338 1108 12-13 0.045375124096473485 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10-11 -0.34142354172891487 2215 12-13 -0.31455591507114633 2215 14-15 -0.34399800329878616 2215 16-17 -0.36773989254052 2215 18-19 -0.3992234060243689 2215 20-21 -0.425165578167352 2215 22-23 -0.3561056364071362 2215 24-25 -0.3487581150025889 2215 26-27 -0.40240639796529365 2215 28-29 -0.409213833049634 2215 30-31 -0.3743824743334656 2215 32-33 -0.26927090473305526 2215 34-35 -0.3441518591214532 2215 36-37 -0.31549135727463096 2215 38-39 -0.3097999994326841 2215 40-41 -0.31608492935793464 2215 42-43 -0.2985478600544056 2215 44-45 -0.3417069689614394 2215 46-47 -0.3516096196072027 2215 48-49 -0.3028922233537088 2215 50-51 -0.27015472031251164 2215 52-53 -0.19930518240755646 2215 54-55 -0.12472038912741823 2215 56-57 -0.1936400614538485 2215 58-59 -0.19746702774037317 2215 60-61 -0.19713762941877633 2215 62-63 -0.21782955179682517 2215 64-65 -0.1659080727801694 2215 66-67 -0.13537470655251838 2215 68-69 -0.13110596709417877 2215 70-71 -0.19884629839976498 2215 72-73 -0.13821873302241272 2215 74-75 -0.10188539184190404 2215 76-77 -0.04034905991500182 2215 78-79 -0.10877262815972699 2215 80-81 -0.17034905061787953 2215 82-83 -0.11838243068174847 2215 84-85 -0.14090418430673424 2215 86-87 -0.10182833452503814 2215 88-89 -0.166048144830917 2215 90-91 -0.1668403963731251 2215 92-93 -0.16157087161487738 2215 94-95 -0.17293997618842027 2215 96-97 -0.18606463849943822 2215 98-99 -0.11185359754491131 2215 100 -0.06652180533356145 2216 1 -0.38391709385159345 2216 2 -0.43006119990873515 2216 3 -0.5268038028343547 2216 4 -0.26377601654938587 2216 5 -0.4789433348123211 2216 6 -0.31969985185551053 2216 7 -0.5647272578238969 2216 8 -0.34127808491626155 2216 9 -0.5519638217428664 2216 10-11 -0.39866773151759105 2216 12-13 -0.3920467382440407 2216 14-15 -0.6410474651805416 2216 16-17 -0.5039802169509002 2216 18-19 -0.4758142102931586 2216 20-21 -0.5133868993562771 2216 22-23 -0.49487445301624433 2216 24-25 -0.4687452085204953 2216 26-27 -0.5888268490351578 2216 28-29 -0.6001426729488557 2216 30-31 -0.7110260432700954 2216 32-33 -0.6655732865091082 2216 34-35 -0.605978650683511 2216 36-37 -0.6804022330065678 2216 38-39 -0.6037944197052383 2216 40-41 -0.5304116303929831 2216 42-43 -0.5776462345119668 2216 44-45 -0.7708221163545659 2216 46-47 -0.7286709713422255 2216 48-49 -0.77931008744401 2216 50-51 -0.6773128067279188 2216 52-53 -0.6145313897123614 2216 54-55 -0.5890371542717219 2216 56-57 -0.6881860398219075 2216 58-59 -0.7245463043983094 2216 60-61 -0.571686064783826 2216 62-63 -0.5190725355699755 2216 64-65 -0.6572617262484783 2216 66-67 -0.49483450782011573 2216 68-69 -0.5429212311029872 2216 70-71 -0.5745722088182319 2216 72-73 -0.5512463389012794 2216 74-75 -0.49839030259111183 2216 76-77 -0.5235962553154394 2216 78-79 -0.6556900914928292 2216 80-81 -0.8539950601932773 2216 82-83 -0.7173552976552884 2216 84-85 -0.8076491813121436 2216 86-87 -0.607932773005512 2216 88-89 -0.9059227137620596 2216 90-91 -0.7582708422770779 2216 92-93 -0.8313398833706387 2216 94-95 -0.7842972157932984 2216 96-97 -0.8037868721199359 2216 98-99 -0.888344856668219 2216 100 -0.8629201975739598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 346.0 3 34.0 4 54.0 5 100.0 6 271.0 7 772.0 8 1797.0 9 3289.0 10 4731.0 11 6568.0 12 8961.0 13 12888.0 14 15566.0 15 19919.0 16 24586.0 17 33946.0 18 34491.0 19 19515.0 20 14613.0 21 14591.0 22 15187.0 23 16994.0 24 20204.0 25 24395.0 26 31696.0 27 40597.0 28 52600.0 29 69143.0 30 89829.0 31 116457.0 32 150952.0 33 197927.0 34 268268.0 35 391443.0 36 637252.0 37 1168535.0 38 1674026.0 39 691189.0 40 2310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.64555785324474 20.503737613504917 14.694713608536189 23.15599092471415 2 15.482734806864892 20.586816772242265 39.93073909274304 23.999709328149798 3 17.79413761848537 25.479157568989464 31.090621884595105 25.636082927930058 4 12.49214606581716 17.27874029362652 38.3250545095367 31.90405913101962 5 12.539716360094092 37.898639934840496 35.758815202478125 13.802828502587285 6 30.47353984195484 38.41240413189695 17.221558321060332 13.89249770508788 7 26.305581205852512 33.23558613093643 22.484999256302114 17.973833406908938 8 24.919345547212192 36.37834490272538 20.2061336696623 18.496175880400127 9 24.768458087944232 16.528745029392233 21.644110099961843 37.05868678270169 10-11 23.754289026524997 27.121351413077033 28.707487114625795 20.41687244577217 12-13 24.38357960240828 24.703508775663522 29.142055720728106 21.770855901200097 14-15 21.65491284381304 26.601942144115466 26.867061553363015 24.876083458708482 16-17 20.598270060016592 29.988442900850607 27.597394640814343 21.81589239831846 18-19 20.473899088501778 28.33421828306138 30.56560200407077 20.62628062436607 20-21 21.55419178705107 27.469470074904212 30.447071649298387 20.529266488746327 22-23 21.18873554681876 27.331799194083363 30.53789438537029 20.94157087372759 24-25 21.04761476873687 27.47401563707766 30.476976477671847 21.001393116513622 26-27 20.842491291324407 27.461603798614608 30.559839905705154 21.136065004355828 28-29 20.893339597211956 27.455759371758276 30.66233538917551 20.988565641854258 30-31 21.15761275321258 27.74123768026806 30.28488909454512 20.816260471974243 32-33 20.83911565143924 27.564717786145295 30.599419012644844 20.99674754977062 34-35 21.013100832040088 27.315228847187704 30.590955914349788 21.08071440642242 36-37 21.268029580475826 27.635679006841002 30.06802372619102 21.028267686492157 38-39 21.191012834528927 27.760769867515855 30.085232803678135 20.96298449427708 40-41 21.064898172849862 27.53534866021924 30.218866936826117 21.180886230104782 42-43 21.033694632922682 27.57980330190044 30.053054708892603 21.33344735628428 44-45 21.171350783216145 27.6954857941453 29.840242422262115 21.292921000376445 46-47 21.323526721375323 27.8063330284509 29.47544309424271 21.39469715593107 48-49 21.352978126420506 27.85015230617191 29.214976940918753 21.581892626488834 50-51 21.407432840354883 27.879673605464927 29.02991694629909 21.682976607881102 52-53 21.340825381860046 27.968199496001322 29.01482001171714 21.676155110421487 54-55 21.401659388215258 28.232616935848252 28.845889080382797 21.519834595553693 56-57 21.341133632424974 27.999601430493527 28.876156523793174 21.78310841328833 58-59 21.262606717387953 27.676354493524038 29.150648209319414 21.9103905797686 60-61 21.401357361681946 27.987537166878703 28.911434528558942 21.69967094288041 62-63 21.199042816126042 28.0095412055881 28.948745121457893 21.84267085682797 64-65 21.20122664692087 27.822555357849875 29.04579010430277 21.930427890926484 66-67 21.17185877258601 27.846679574070976 28.900593273723736 22.080868379619282 68-69 21.494477449489686 27.96111303467599 28.72380627246284 21.82060324337148 70-71 21.505049336115263 27.788418010830462 28.738865762880316 21.967666890173962 72-73 21.300547202292453 27.884988726562316 28.79889577577734 22.015568295367896 74-75 21.308934364377315 27.926972596599107 28.676072407252164 22.088020631771414 76-77 21.415805210314026 27.90122156249516 28.50642078053451 22.176552446656306 78-79 21.395713437079873 27.95799025039286 28.608843368673327 22.037452943853943 80-81 21.3238400659237 27.878862812715738 28.5924065717096 22.204890549650962 82-83 21.213115761257058 27.873345474877887 28.701285583814823 22.212253180050233 84-85 21.291502528048152 27.700503261045117 28.60140707506301 22.406587135843722 86-87 21.326843387830518 27.838722891455543 28.542624772356383 22.291808948357552 88-89 21.169860851399676 27.692081868135375 28.621307754327518 22.51674952613743 90-91 21.11651852717916 27.60231189161251 28.648035928569694 22.633133652638644 92-93 21.151961170154152 27.758259702674952 28.523896582519164 22.56588254465173 94-95 21.06094082998593 27.53556629731412 28.640373326457702 22.76311954624225 96-97 21.1034469847818 27.554358000360796 28.593907955601395 22.748287059256008 98-99 21.006644208146554 27.628523744443267 28.56586586887268 22.79896617853749 100 21.077225979809608 27.45827364808117 28.564985858488217 22.899514513621003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 908.0 1 672.5 2 754.5 3 1087.5 4 704.5 5 444.5 6 1236.5 7 3261.0 8 5412.5 9 6190.5 10 4743.0 11 2958.5 12 2780.5 13 3181.0 14 3610.5 15 4147.5 16 4873.0 17 5631.0 18 6474.0 19 7521.0 20 8977.5 21 11215.0 22 14268.5 23 18643.0 24 25113.0 25 33353.5 26 44170.0 27 57978.5 28 73790.0 29 91476.0 30 108792.0 31 123417.0 32 135627.0 33 147416.0 34 159675.0 35 171249.5 36 180523.5 37 190112.0 38 200877.0 39 207802.0 40 211965.0 41 214488.5 42 214838.5 43 213189.0 44 208740.5 45 204260.0 46 201206.5 47 195378.5 48 187703.0 49 182693.5 50 178381.0 51 170922.0 52 158726.5 53 147581.0 54 139599.0 55 132250.0 56 122388.0 57 110282.5 58 100284.0 59 90744.0 60 79162.0 61 66994.5 62 56909.0 63 47626.0 64 38677.0 65 31254.0 66 25174.0 67 19610.0 68 14853.0 69 11388.5 70 8366.5 71 5980.5 72 4409.5 73 3122.5 74 2226.0 75 1548.0 76 953.0 77 601.5 78 418.0 79 252.0 80 133.5 81 68.0 82 31.0 83 16.5 84 13.0 85 7.5 86 3.0 87 1.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042681791586921945 2 0.0 3 0.0 4 1.0210954925100944E-4 5 0.0 6 0.0 7 0.0 8 8.338946522165771E-4 9 0.0 10-11 0.0 12-13 0.04347314059361727 14-15 1.786917111892665E-4 16-17 0.0 18-19 3.4036516417003145E-5 20-21 6.807303283400629E-5 22-23 0.0 24-25 1.3614606566801258E-4 26-27 8.509129104250786E-5 28-29 3.9992906789978696E-4 30-31 1.7018258208501572E-5 32-33 1.786917111892665E-4 34-35 5.105477462550472E-5 36-37 6.807303283400629E-5 38-39 3.063286477530283E-4 40-41 1.0210954925100944E-4 42-43 5.445842626720503E-4 44-45 2.3825561491902202E-4 46-47 1.1061867835526023E-4 48-49 8.338946522165771E-4 50-51 4.1694732610828857E-4 52-53 5.871299081933042E-4 54-55 3.57383422378533E-4 56-57 4.7651122983804404E-4 58-59 1.3614606566801258E-4 60-61 1.786917111892665E-4 62-63 9.360042014675866E-4 64-65 3.9992906789978696E-4 66-67 0.0014720793350353862 68-69 0.0015061158514523892 70-71 3.744016805870346E-4 72-73 0.0010466228798228466 74-75 9.530224596760881E-4 76-77 7.317851029655676E-4 78-79 0.003667434643932089 80-81 0.0026973939260474993 82-83 0.0012508419783248657 84-85 0.0028080126044027595 86-87 0.0013784789148886276 88-89 0.002203864438000954 90-91 0.002739939571568753 92-93 0.0025442296021709852 94-95 0.0037355076767660953 96-97 0.003097322993947286 98-99 0.0029356495409665214 100 0.0013444423984716243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5876042.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.566556526323275 #Duplication Level Percentage of deduplicated Percentage of total 1 74.71295983881726 43.75680785649011 2 14.880015435442118 17.429425302247672 3 4.713848997994159 8.282217113927317 4 1.9636457607223368 4.600158817720792 5 1.0893962086704643 3.1901092317330506 6 0.6256658576663112 2.198585689176273 7 0.40470541755122724 1.6591541899466253 8 0.28069713029221854 1.3151571478428759 9 0.21109618682960143 1.1126859082602434 >10 1.0375166989237967 10.692291352414282 >50 0.05742297175023176 2.304718473494252 >100 0.02195348478026416 2.201658451287036 >500 6.397962463149356E-4 0.25411801583672766 >1k 3.4897145104005074E-4 0.337023546887786 >5k 2.908095423536967E-5 0.1486017002931885 >10k+ 5.816190847073934E-5 0.5172872024418019 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15670 0.26667610612721965 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14702 0.25020243218139016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8725 0.14848430286917624 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7018258208501572E-5 0.0 0.0 0.0 0.0 2 1.7018258208501572E-5 0.0 0.0 0.0 0.0 3 1.7018258208501572E-5 1.7018258208501572E-5 0.0 1.7018258208501572E-5 0.0 4 1.7018258208501572E-5 1.7018258208501572E-5 0.0 1.7018258208501572E-5 0.0 5 1.7018258208501572E-5 1.7018258208501572E-5 0.0 3.4036516417003145E-5 0.0 6 1.7018258208501572E-5 1.7018258208501572E-5 0.0 3.4036516417003145E-5 0.0 7 1.7018258208501572E-5 1.7018258208501572E-5 0.0 3.4036516417003145E-5 0.0 8 1.7018258208501572E-5 1.7018258208501572E-5 0.0 3.4036516417003145E-5 0.0 9 1.7018258208501572E-5 1.7018258208501572E-5 0.0 5.105477462550472E-5 0.0 10-11 1.7018258208501572E-5 2.552738731275236E-5 0.0 5.105477462550472E-5 0.0 12-13 1.7018258208501572E-5 3.4036516417003145E-5 0.0 5.9563903729755505E-5 0.0 14-15 1.7018258208501572E-5 3.4036516417003145E-5 0.0 7.658216193825708E-5 0.0 16-17 1.7018258208501572E-5 3.4036516417003145E-5 0.0 1.1912780745951101E-4 0.0 18-19 1.7018258208501572E-5 6.807303283400629E-5 0.0 1.3614606566801258E-4 0.0 20-21 1.7018258208501572E-5 6.807303283400629E-5 0.0 1.872008402935173E-4 0.0 22-23 1.7018258208501572E-5 6.807303283400629E-5 0.0 3.4036516417003144E-4 0.0 24-25 1.7018258208501572E-5 6.807303283400629E-5 0.0 6.977485865485645E-4 0.0 26-27 1.7018258208501572E-5 6.807303283400629E-5 0.0 0.0013104058820546211 0.0 28-29 1.7018258208501572E-5 6.807303283400629E-5 0.0 0.004884240105839951 0.0 30-31 1.7018258208501572E-5 6.807303283400629E-5 0.0 0.015631270164508694 0.0 32-33 1.7018258208501572E-5 6.807303283400629E-5 0.0 0.03996737940266594 0.0 34-35 1.7018258208501572E-5 7.658216193825708E-5 0.0 0.07882006289267504 0.0 36-37 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.13954971730971288 0.0 38-39 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.22139052103439696 0.0 40-41 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.30420136547696564 0.0 42-43 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.3861442787509007 0.0 44-45 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.4857010892706349 0.0 46-47 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.6049735519249182 0.0 48-49 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.7269519176343532 0.0 50-51 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.848921774214684 3.4036516417003145E-5 52-53 1.7018258208501572E-5 8.509129104250786E-5 0.0 0.9681431820943417 3.4036516417003145E-5 54-55 1.7018258208501572E-5 8.509129104250786E-5 0.0 1.0983668939057958 3.4036516417003145E-5 56-57 1.7018258208501572E-5 8.509129104250786E-5 0.0 1.2509781243905336 3.4036516417003145E-5 58-59 1.7018258208501572E-5 8.509129104250786E-5 0.0 1.415110715682427 3.4036516417003145E-5 60-61 1.7018258208501572E-5 8.509129104250786E-5 0.0 1.5840169284018053 3.4036516417003145E-5 62-63 1.7018258208501572E-5 8.509129104250786E-5 0.0 1.747043332910146 3.4036516417003145E-5 64-65 1.7018258208501572E-5 8.509129104250786E-5 0.0 1.9225526298144229 3.4036516417003145E-5 66-67 1.7018258208501572E-5 8.509129104250786E-5 0.0 2.1176754693039976 3.4036516417003145E-5 68-69 1.7018258208501572E-5 8.509129104250786E-5 0.0 2.3332712734184 3.4036516417003145E-5 70-71 1.7018258208501572E-5 8.509129104250786E-5 0.0 2.5602012374996637 3.4036516417003145E-5 72-73 1.7018258208501572E-5 8.509129104250786E-5 0.0 2.7893776116644506 3.4036516417003145E-5 74-75 1.7018258208501572E-5 8.509129104250786E-5 0.0 3.022936187317926 3.4036516417003145E-5 76-77 1.7018258208501572E-5 8.509129104250786E-5 0.0 3.2729599277881265 3.4036516417003145E-5 78-79 1.7018258208501572E-5 8.509129104250786E-5 0.0 3.5479154165337823 3.4036516417003145E-5 80-81 1.7018258208501572E-5 8.509129104250786E-5 0.0 3.8405613846871756 3.4036516417003145E-5 82-83 1.7018258208501572E-5 8.509129104250786E-5 0.0 4.138551085918038 3.4036516417003145E-5 84-85 1.7018258208501572E-5 8.509129104250786E-5 0.0 4.438404286422731 3.4036516417003145E-5 86-87 1.7018258208501572E-5 8.509129104250786E-5 0.0 4.759862165723118 3.4036516417003145E-5 88 1.7018258208501572E-5 8.509129104250786E-5 0.0 5.017714304969229 3.4036516417003145E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9735 0.0 61.046864 1 GTATCAA 17725 0.0 40.23941 1 ATCAACG 21955 0.0 32.0444 3 TCAACGC 22040 0.0 32.006107 4 CAACGCA 22985 0.0 30.751554 5 TATCAAC 23010 0.0 30.738565 2 AACGCAG 23870 0.0 29.698023 6 GTGGTAT 4105 0.0 26.801245 1 ACGCAGA 27520 0.0 25.605534 7 CGCAGAG 27785 0.0 25.22588 8 GTACATG 16545 0.0 25.092628 1 TGGTATC 4240 0.0 23.941517 2 TACATGG 16935 0.0 23.088825 2 GCAGAGT 30275 0.0 22.77805 9 ACATGGG 17195 0.0 21.892435 3 GAGTACT 21735 0.0 21.243057 12-13 CAGAGTA 29770 0.0 20.86538 10-11 AGAGTAC 28495 0.0 19.809763 10-11 GTACTTT 23580 0.0 19.262741 14-15 CATGGGG 11965 0.0 18.578566 4 >>END_MODULE