FastQCFastQC Report
Wed 25 May 2016
SRR2049387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5040691
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.041.2527961
GTATCAA77150.035.034991
TCAACGC79050.033.1212044
ATCAACG79600.032.8923533
CAACGCA84100.031.2438345
AACGCAG89400.029.4412296
TATCAAC93750.028.0782222
ACGCAGA106800.024.3803337
CGCAGAG108950.023.55418
GTACATG121800.022.7320211
TACATGG123300.020.8534092
GCAGAGT126350.019.4548349
ACATGGG129300.019.1950053
CCGGTCG5000.018.800149
GAGTACA96600.018.0537261
AGAGTAC108800.017.58193210-11
GTATAGA24000.017.2361981
CATGGGG96150.016.7686334
CAGAGTA122750.016.52191510-11
AGTACAT103600.015.6988982
Produced by FastQC (version 0.11.5)