##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049387_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5040691
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.594180440737194	31.0	31.0	34.0	27.0	34.0
2	30.836663862156993	31.0	31.0	34.0	27.0	34.0
3	31.003975447017087	31.0	31.0	34.0	28.0	34.0
4	34.61890899481837	37.0	35.0	37.0	32.0	37.0
5	34.644534846512116	37.0	35.0	37.0	32.0	37.0
6	34.64059312502988	37.0	35.0	37.0	32.0	37.0
7	34.55015631785404	37.0	35.0	37.0	32.0	37.0
8	34.51755146268636	37.0	35.0	37.0	31.0	37.0
9	36.11869582959955	39.0	37.0	39.0	32.0	39.0
10-11	36.0760305481927	39.0	37.0	39.0	31.0	39.0
12-13	36.008961469766746	39.0	37.0	39.0	31.0	39.0
14-15	37.20125445102666	40.0	37.0	41.0	31.0	41.0
16-17	37.160769327062496	40.0	37.0	41.0	31.0	41.0
18-19	37.189434147024684	40.0	37.5	41.0	31.0	41.0
20-21	37.253984126382676	40.0	38.0	41.0	31.0	41.0
22-23	37.20605210674489	40.0	37.0	41.0	31.0	41.0
24-25	37.20576365422915	40.0	37.0	41.0	31.0	41.0
26-27	37.18564766219552	40.0	37.0	41.0	31.0	41.0
28-29	37.12400799810978	40.0	37.0	41.0	31.0	41.0
30-31	37.05878211935625	40.0	37.0	41.0	31.0	41.0
32-33	36.99663885368098	40.0	37.0	41.0	30.5	41.0
34-35	36.897301580279375	40.0	37.0	41.0	30.0	41.0
36-37	36.81670846318491	40.0	37.0	41.0	30.0	41.0
38-39	36.71454072467446	40.0	37.0	41.0	30.0	41.0
40-41	36.611551372619346	40.0	37.0	41.0	30.0	41.0
42-43	36.47567406928931	40.0	36.0	41.0	30.0	41.0
44-45	36.35306568087589	40.0	36.0	41.0	30.0	41.0
46-47	36.23441994361487	40.0	36.0	41.0	29.5	41.0
48-49	36.1072261719673	40.0	36.0	41.0	29.0	41.0
50-51	35.96798355622275	39.5	35.0	41.0	28.5	41.0
52-53	35.82436475475287	39.0	35.0	41.0	28.0	41.0
54-55	35.686027570426354	39.0	35.0	41.0	28.0	41.0
56-57	35.56028360000643	39.0	35.0	41.0	28.0	41.0
58-59	35.36183858522571	39.0	35.0	41.0	27.5	41.0
60-61	35.14508080340572	39.0	35.0	40.0	27.0	41.0
62-63	34.939830471655576	38.0	34.0	40.0	26.5	41.0
64-65	34.69636037995584	38.0	34.0	40.0	26.0	41.0
66-67	34.43953051278089	37.5	34.0	40.0	26.0	41.0
68-69	34.15517416163776	37.0	34.0	40.0	26.0	41.0
70-71	33.87340188081356	36.5	34.0	39.5	26.0	41.0
72-73	33.535762259579094	36.0	33.0	39.0	25.5	41.0
74-75	33.19101458510351	35.5	33.0	39.0	25.0	40.5
76-77	32.86013286670419	35.0	33.0	38.0	24.0	40.0
78-79	32.541410592317604	35.0	33.0	37.0	24.0	39.0
80-81	32.187460211308334	35.0	33.0	37.0	23.0	39.0
82-83	31.87527404476886	35.0	32.0	36.0	22.5	39.0
84-85	31.584726578161607	35.0	32.0	36.0	21.5	37.5
86-87	31.26670033929872	35.0	32.0	36.0	20.0	37.0
88-89	31.019140629727154	35.0	32.0	35.0	19.5	37.0
90-91	30.816716299412125	35.0	32.0	35.0	18.5	36.0
92-93	30.58293287567121	35.0	32.0	35.0	16.5	36.0
94-95	30.382815173554576	35.0	32.0	35.0	9.0	36.0
96-97	30.134766146149406	34.5	31.5	35.0	2.0	36.0
98-99	29.933274227680293	34.0	31.0	35.0	2.0	35.0
100	29.812705837354443	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16457273546645368
1101	2	0.13459471941507317
1101	3	0.22055004210409024
1101	4	0.26670175723678113
1101	5	0.2486949082852732
1101	6	0.30962388107879235
1101	7	0.31270622209387255
1101	8	0.35522735493891133
1101	9	0.4768766063841028
1101	10-11	0.45655959075730124
1101	12-13	0.43943700166731503
1101	14-15	0.5041997987856348
1101	16-17	0.49102069997051245
1101	18-19	0.4587243116702666
1101	20-21	0.4343005922639591
1101	22-23	0.44208240652114483
1101	24-25	0.45666489147474465
1101	26-27	0.36768802346146146
1101	28-29	0.4257362239480287
1101	30-31	0.42794537234822627
1101	32-33	0.4369594298817958
1101	34-35	0.4620474481581951
1101	36-37	0.45289066376667364
1101	38-39	0.4575671241577055
1101	40-41	0.5237371444895018
1101	42-43	0.4597721387597318
1101	44-45	0.39618454799823155
1101	46-47	0.40743855328708634
1101	48-49	0.2927983249672579
1101	50-51	0.3993909740046462
1101	52-53	0.4079535119453226
1101	54-55	0.38846945459958704
1101	56-57	0.30366419134195866
1101	58-59	0.40095170472319097
1101	60-61	0.4513644294167918
1101	62-63	0.4316230618757544
1101	64-65	0.3797348349138545
1101	66-67	0.3399386177844903
1101	68-69	0.37972958283692293
1101	70-71	0.32811812042844224
1101	72-73	0.4161308185951711
1101	74-75	0.35330137179649057
1101	76-77	0.39802527954550726
1101	78-79	0.37782005379469297
1101	80-81	0.433372048604717
1101	82-83	0.4667145414666791
1101	84-85	0.3804418904839757
1101	86-87	0.4057970837723701
1101	88-89	0.3925201817703545
1101	90-91	0.41503065890676183
1101	92-93	0.3401371263240378
1101	94-95	0.29844006920697197
1101	96-97	0.37220238317804544
1101	98-99	0.4548985784406341
1101	100	0.4766782423692355
1102	1	0.03838591613371278
1102	2	0.16363200350271612
1102	3	0.18844776641222083
1102	4	0.23808880742632255
1102	5	0.19637543955299464
1102	6	0.36148660006535493
1102	7	0.3460119878637258
1102	8	0.3709634855040562
1102	9	0.4558192811133992
1102	10-11	0.4058368188812551
1102	12-13	0.44477951838059937
1102	14-15	0.517782566391638
1102	16-17	0.45913317675767473
1102	18-19	0.41961953327484025
1102	20-21	0.3770113941077682
1102	22-23	0.39443135753570857
1102	24-25	0.46112048599633937
1102	26-27	0.3896926456741241
1102	28-29	0.41593243436540916
1102	30-31	0.3996426727921474
1102	32-33	0.483830260726549
1102	34-35	0.48053551850427567
1102	36-37	0.4540932795295163
1102	38-39	0.4544218741849022
1102	40-41	0.47299967847850155
1102	42-43	0.4218649276623623
1102	44-45	0.3467897197884682
1102	46-47	0.24274536315842
1102	48-49	0.33044819597757424
1102	50-51	0.4078171531807726
1102	52-53	0.4228434464816857
1102	54-55	0.3008751778100276
1102	56-57	0.2797938370459576
1102	58-59	0.30994895269879663
1102	60-61	0.27033266799238476
1102	62-63	0.3383036285861394
1102	64-65	0.27423846694112797
1102	66-67	0.35677458037771714
1102	68-69	0.3692895174682391
1102	70-71	0.3943049416414368
1102	72-73	0.3966428231769541
1102	74-75	0.39940208143013933
1102	76-77	0.39342415386008156
1102	78-79	0.37671261616508644
1102	80-81	0.30643403275291803
1102	82-83	0.35122238242818327
1102	84-85	0.4340399181794581
1102	86-87	0.3963614654712835
1102	88-89	0.374243460655169
1102	90-91	0.4164712952126308
1102	92-93	0.4292852773308624
1102	94-95	0.43304806625723913
1102	96-97	0.413832888766283
1102	98-99	0.49062282208048913
1102	100	0.485170737878029
1103	1	0.06683135867359624
1103	2	0.15920120737552068
1103	3	0.19588718970682706
1103	4	0.1928170834719296
1103	5	0.20300764282293926
1103	6	0.2772529850626242
1103	7	0.32600133304446643
1103	8	0.3054840935693832
1103	9	0.45943833233855713
1103	10-11	0.3683488885391739
1103	12-13	0.37621861255372124
1103	14-15	0.43472902130949365
1103	16-17	0.43271593810428044
1103	18-19	0.43099319265321867
1103	20-21	0.3748609099350304
1103	22-23	0.33916864098322463
1103	24-25	0.3640946897066115
1103	26-27	0.41987546013191945
1103	28-29	0.43374154629559314
1103	30-31	0.41639136160937795
1103	32-33	0.42357974469143045
1103	34-35	0.41573249619025887
1103	36-37	0.39074124964419354
1103	38-39	0.4020356764799047
1103	40-41	0.33758612568162505
1103	42-43	0.385152235511633
1103	44-45	0.326260170131043
1103	46-47	0.3821707808353736
1103	48-49	0.3553519355461958
1103	50-51	0.3092859301874995
1103	52-53	0.31783153121258323
1103	54-55	0.27215672226869003
1103	56-57	0.2445215762601194
1103	58-59	0.3167237982031992
1103	60-61	0.20856995603717365
1103	62-63	0.3071007619713413
1103	64-65	0.2996567393661138
1103	66-67	0.33415886315460597
1103	68-69	0.3238317939043682
1103	70-71	0.2509924823517622
1103	72-73	0.22867324823524626
1103	74-75	0.23821075470061714
1103	76-77	0.3508859264290294
1103	78-79	0.23849422762813077
1103	80-81	0.12182966015757302
1103	82-83	0.14371030227255943
1103	84-85	0.2684317047957947
1103	86-87	0.2866569899186864
1103	88-89	0.20618784969500226
1103	90-91	0.2946269718450587
1103	92-93	0.28793089537806793
1103	94-95	0.32173457145842477
1103	96-97	0.23579942545192623
1103	98-99	0.31845472201712965
1103	100	0.2371104261132544
1104	1	-0.01463140587997458
1104	2	0.05366352009986741
1104	3	0.1277624531980308
1104	4	0.1596343085404328
1104	5	0.20003217867586898
1104	6	0.28207682557181357
1104	7	0.26744460887493204
1104	8	0.28883284958286737
1104	9	0.33536007025268333
1104	10-11	0.35889217082434755
1104	12-13	0.2992789357453347
1104	14-15	0.3951941393794556
1104	16-17	0.40315225993717974
1104	18-19	0.391310069573791
1104	20-21	0.3860541318246149
1104	22-23	0.4628104930265877
1104	24-25	0.4359283777685903
1104	26-27	0.39393105374828963
1104	28-29	0.4101557843626935
1104	30-31	0.36279803815271805
1104	32-33	0.4173660427707446
1104	34-35	0.4201607060994448
1104	36-37	0.41588354364569824
1104	38-39	0.5207868657344648
1104	40-41	0.5021384745144957
1104	42-43	0.5270556819730885
1104	44-45	0.5193126412672555
1104	46-47	0.43807297332022443
1104	48-49	0.4520842910690064
1104	50-51	0.5097706034878087
1104	52-53	0.510506853762223
1104	54-55	0.5421894046291129
1104	56-57	0.512607224010587
1104	58-59	0.5465233425655569
1104	60-61	0.49496506592044653
1104	62-63	0.5039715890792493
1104	64-65	0.559372345284217
1104	66-67	0.5496433530349307
1104	68-69	0.6008874838512313
1104	70-71	0.5923180263565229
1104	72-73	0.622634953923459
1104	74-75	0.633895383084031
1104	76-77	0.6603558650841421
1104	78-79	0.6175310599006423
1104	80-81	0.6596934600399251
1104	82-83	0.6001892735916883
1104	84-85	0.5689768611851349
1104	86-87	0.49515982921800017
1104	88-89	0.5163291964933201
1104	90-91	0.5055162311209642
1104	92-93	0.49370707763536714
1104	94-95	0.49209457797753586
1104	96-97	0.4569288227884698
1104	98-99	0.47541719400883053
1104	100	0.4702713516644863
1105	1	0.0718196294676865
1105	2	0.09228655764348304
1105	3	0.11073108684529487
1105	4	0.15251489619635095
1105	5	0.1375901887117692
1105	6	0.2081284832077941
1105	7	0.21165521362907924
1105	8	0.24435317457593442
1105	9	0.32557124579263785
1105	10-11	0.2558622917589517
1105	12-13	0.2726109460316408
1105	14-15	0.29724656042877484
1105	16-17	0.3309849872541406
1105	18-19	0.23780037052389957
1105	20-21	0.2634524840481234
1105	22-23	0.2572324811556612
1105	24-25	0.2598287913601851
1105	26-27	0.23496903229465005
1105	28-29	0.20879580861348046
1105	30-31	0.22703045037159342
1105	32-33	0.2922969629879617
1105	34-35	0.3290742158289106
1105	36-37	0.23839465648031677
1105	38-39	0.2659149605112674
1105	40-41	0.30543701830102066
1105	42-43	0.3060417666612736
1105	44-45	0.2556149732263364
1105	46-47	0.21452357272135458
1105	48-49	0.20144261326289126
1105	50-51	0.2224608295159456
1105	52-53	0.29026448776889424
1105	54-55	0.25695544581388674
1105	56-57	0.27136065011942634
1105	58-59	0.3216129270426151
1105	60-61	0.24484386264719404
1105	62-63	0.17554579706325768
1105	64-65	0.2427992043553786
1105	66-67	0.3375828923205617
1105	68-69	0.2583282293400728
1105	70-71	0.26797441735797634
1105	72-73	0.3037673655847257
1105	74-75	0.24760349356504463
1105	76-77	0.3581723559274934
1105	78-79	0.2607221580752892
1105	80-81	0.2834764215260819
1105	82-83	0.3268228691519326
1105	84-85	0.3520678133176993
1105	86-87	0.34880898046559494
1105	88-89	0.2660925941186285
1105	90-91	0.3320744484849172
1105	92-93	0.3024535083231612
1105	94-95	0.2990943584835257
1105	96-97	0.27771277630643354
1105	98-99	0.28269950334939153
1105	100	0.2529916478648069
1106	1	0.1219703932534415
1106	2	0.10062279624521508
1106	3	0.17492374687595813
1106	4	0.21053581495594642
1106	5	0.21084356409601668
1106	6	0.29042933998281484
1106	7	0.2818962909398266
1106	8	0.21517410623864208
1106	9	0.3312386076559122
1106	10-11	0.3217876910834434
1106	12-13	0.29030917942166923
1106	14-15	0.34377378248350254
1106	16-17	0.42251508562797824
1106	18-19	0.42181200648570183
1106	20-21	0.36630601592398904
1106	22-23	0.37996801571056693
1106	24-25	0.3924933546538867
1106	26-27	0.347484266860441
1106	28-29	0.4309421009740575
1106	30-31	0.3983861582004309
1106	32-33	0.4686202455401016
1106	34-35	0.44386581606728726
1106	36-37	0.3422376752134113
1106	38-39	0.3835446667875999
1106	40-41	0.43957224706868203
1106	42-43	0.456320941242474
1106	44-45	0.3866693354506552
1106	46-47	0.33241733791197703
1106	48-49	0.3968050604872957
1106	50-51	0.4347026586812035
1106	52-53	0.3995566734033389
1106	54-55	0.46399521097721674
1106	56-57	0.33297496198851206
1106	58-59	0.41313673928411987
1106	60-61	0.44899618390494567
1106	62-63	0.37243027407978957
1106	64-65	0.4197785606140769
1106	66-67	0.4489616603296085
1106	68-69	0.5330415176034862
1106	70-71	0.4756610751661583
1106	72-73	0.4851720349403763
1106	74-75	0.4911999304859833
1106	76-77	0.5571776168412725
1106	78-79	0.42645161203785165
1106	80-81	0.4555667797156886
1106	82-83	0.513357394699888
1106	84-85	0.48782751102089605
1106	86-87	0.474260289614687
1106	88-89	0.4566467290909877
1106	90-91	0.47573159621027017
1106	92-93	0.4739591734931494
1106	94-95	0.52492362133939
1106	96-97	0.5170718224023929
1106	98-99	0.5518319054253027
1106	100	0.6270845941212677
1107	1	0.13870167177989856
1107	2	0.11722642504829395
1107	3	0.11023665827921647
1107	4	0.16380289317745422
1107	5	0.16790840225539938
1107	6	0.2506249469238284
1107	7	0.2010466811854954
1107	8	0.3406178479325632
1107	9	0.39581486502088126
1107	10-11	0.28935929059672105
1107	12-13	0.34969358978038656
1107	14-15	0.34458811610866746
1107	16-17	0.4582458724927676
1107	18-19	0.3960989307736682
1107	20-21	0.3443297331177675
1107	22-23	0.3841657814626487
1107	24-25	0.39986285141225864
1107	26-27	0.3960701380299696
1107	28-29	0.45742421206922046
1107	30-31	0.3942902642199968
1107	32-33	0.46042337425585345
1107	34-35	0.46412089707074244
1107	36-37	0.4116181084258841
1107	38-39	0.4485185730785446
1107	40-41	0.48029122773012034
1107	42-43	0.4349704984882905
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2215	16-17	-0.44041069383217035
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2215	26-27	-0.4482636612431392
2215	28-29	-0.4593634941722584
2215	30-31	-0.41390310939264197
2215	32-33	-0.46111011014382086
2215	34-35	-0.39044758521063727
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2216	1	-0.9922829846001378
2216	2	-0.9408293670963559
2216	3	-1.0701599446026684
2216	4	-1.2450984254387762
2216	5	-1.2348038945045658
2216	6	-2.5635314043918243
2216	7	-2.5154124651923766
2216	8	-3.2504798582224588
2216	9	-2.8041110553449613
2216	10-11	-1.9041781893631153
2216	12-13	-1.6629116900954841
2216	14-15	-1.925075177357101
2216	16-17	-2.3661331804871253
2216	18-19	-1.9140167489730402
2216	20-21	-1.7690612618212995
2216	22-23	-1.9694688048550617
2216	24-25	-2.081154360305554
2216	26-27	-2.1311132151046976
2216	28-29	-2.4864434929287356
2216	30-31	-2.1378122606975722
2216	32-33	-2.4085327320823424
2216	34-35	-3.4431799689260956
2216	36-37	-2.3942099073416045
2216	38-39	-2.2549038490788575
2216	40-41	-2.810158051975364
2216	42-43	-2.6857037092342964
2216	44-45	-2.4640556645572858
2216	46-47	-2.231707082223032
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2216	50-51	-2.749792935409012
2216	52-53	-3.11308519358802
2216	54-55	-2.472816999963875
2216	56-57	-2.4761112759245805
2216	58-59	-3.4827217785804763
2216	60-61	-4.061535122213257
2216	62-63	-3.251976232924335
2216	64-65	-3.2568063613553093
2216	66-67	-2.8736993668657185
2216	68-69	-3.2759806305948054
2216	70-71	-3.1299003237336223
2216	72-73	-3.5755224974366087
2216	74-75	-3.959201547500669
2216	76-77	-4.612428284622261
2216	78-79	-3.685465032577291
2216	80-81	-3.504692466754449
2216	82-83	-3.786240221956895
2216	84-85	-3.8317828219247687
2216	86-87	-3.455515768056209
2216	88-89	-3.4383189324816463
2216	90-91	-3.665149120361985
2216	92-93	-3.6879387048275625
2216	94-95	-3.5121583858583527
2216	96-97	-3.8341842804156165
2216	98-99	-3.624457822653234
2216	100	-3.2840806760196593
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85978.0
3	14839.0
4	9749.0
5	7782.0
6	7048.0
7	7325.0
8	7944.0
9	9074.0
10	10580.0
11	11679.0
12	13106.0
13	14565.0
14	16498.0
15	17352.0
16	17832.0
17	18838.0
18	17875.0
19	16151.0
20	16286.0
21	16973.0
22	18786.0
23	22108.0
24	26792.0
25	33431.0
26	40586.0
27	47787.0
28	58280.0
29	72065.0
30	87108.0
31	104440.0
32	132600.0
33	173984.0
34	238224.0
35	344338.0
36	558439.0
37	971863.0
38	1294556.0
39	476368.0
40	1462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39828570578227	20.316390458286005	14.14346301320939	23.141860822722336
2	15.139384197104555	20.103950227726013	40.91687752408736	23.83978805108207
3	18.081570256511824	25.368063599961182	30.413380298880444	26.13698584464655
4	12.31120050726917	16.77179535777262	39.58485694354447	31.332147191413735
5	12.852812027090724	37.99530625914808	36.02578084298792	13.126100870773277
6	30.7302278337515	38.282909352738436	17.752031586087927	13.234831227422136
7	26.710711588782637	32.64832167214582	22.844826987404726	17.796139751666818
8	24.513813970435887	36.79990442191532	20.708115325596587	17.9781662820522
9	24.465707392933094	17.064657304142326	21.995303654909133	36.47433164801544
10-11	23.544492962365563	27.22754067062561	29.323098097216548	19.904868269792278
12-13	23.835709933016076	24.705221824515284	29.453674639119075	22.005393603349564
14-15	21.28646696991867	27.00561946652825	26.488812318986767	25.219101244566318
16-17	20.899966401910547	30.43630681386321	27.15280303918831	21.510923745037932
18-19	20.822030331109083	29.37098978676298	29.16170604699125	20.64527383513669
20-21	21.900581737225764	28.516465884335645	28.79683611791764	20.786116260520952
22-23	21.477213663248477	28.401279979423798	28.82815478431406	21.29335157301366
24-25	21.39123670115123	28.553404763275037	28.71443965731092	21.34091887826282
26-27	21.172369845998382	28.53731305962544	28.78783037734078	21.502486717035396
28-29	21.140214640125137	28.540323710553757	28.965541603343237	21.35392004597787
30-31	21.40732264537967	28.9011646090927	28.54355992049128	21.147952825036356
32-33	21.123883397883844	28.64801343455023	28.931574606445633	21.296528561120297
34-35	21.315629274507675	28.37698075445169	28.92892031734739	21.378469653693248
36-37	21.5471470570404	28.761724513171433	28.412939812189496	21.27818861759867
38-39	21.38044301613261	28.848925660377585	28.55223277249338	21.21839855099643
40-41	21.316536594407534	28.58234203096888	28.74263196185588	21.358489412767707
42-43	21.227268812356098	28.595315104062273	28.688438702743124	21.488977380838506
44-45	21.32653906662016	28.637566288503642	28.60572498500462	21.430169659871574
46-47	21.376576547971414	28.797125277455578	28.43400451866853	21.39229365590448
48-49	21.305852801576478	28.65062240993276	28.462641760677666	21.5808830278131
50-51	21.361371095174427	28.602525779972858	28.40807396253749	21.628029162315222
52-53	21.359354413767605	28.58668664940007	28.483472074017612	21.57048686281471
54-55	21.46748252126417	28.85740500965311	28.260784792534864	21.41432767654786
56-57	21.357472627383245	28.66675285905887	28.34327340646854	21.63250110708935
58-59	21.29653103613673	28.31961337306421	28.662891035735864	21.72096455506319
60-61	21.466201425134592	28.690602275841552	28.310256805625922	21.532939493397933
62-63	21.334695482146902	28.700062729493002	28.35191503557086	21.613326752789238
64-65	21.37771616343843	28.441026021629096	28.46245786311443	21.718799951818045
66-67	21.289469971199672	28.519764826770395	28.339447755279185	21.851317446750752
68-69	21.58376794775141	28.59297844612802	28.328111874623076	21.49514173149749
70-71	21.526060918287033	28.4815694458391	28.35924548101855	21.633124154855313
72-73	21.353758875268927	28.612193152760845	28.400473605652948	21.633574366317283
74-75	21.431765673006467	28.602709062306054	28.351736561228254	21.61378870345923
76-77	21.463622566264906	28.577396109834	28.221126979691885	21.737854344209204
78-79	21.502550830061615	28.599688756661845	28.35461737152183	21.54314304175471
80-81	21.441607939761198	28.480005731132113	28.388273881437005	21.690112447669684
82-83	21.347139997567076	28.554025046391207	28.497706683727547	21.601128272314167
84-85	21.3831343347855	28.41797295535417	28.387719831265162	21.811172878595176
86-87	21.524489213133123	28.56566511289532	28.285269878552615	21.624575795418938
88-89	21.348442482898765	28.479056817572946	28.357061068323574	21.81543963120472
90-91	21.333387576012104	28.38682438717763	28.43075103453853	21.849037002271743
92-93	21.41543646156562	28.596981266643994	28.269913288239234	21.71766898355115
94-95	21.335072001355794	28.351484534014116	28.42503924058759	21.888404224042496
96-97	21.380511124367672	28.415945353523952	28.3273364703371	21.876207051771274
98-99	21.368627832969725	28.450484268922654	28.31583011138751	21.865057786720115
100	21.45074951033499	28.330917328596417	28.348831539167946	21.869501621900646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1400.0
1	1138.5
2	1069.0
3	1268.0
4	1151.0
5	934.0
6	759.0
7	666.5
8	651.5
9	661.0
10	650.0
11	635.5
12	677.0
13	742.0
14	848.5
15	1024.5
16	1248.5
17	1604.5
18	2247.5
19	3234.0
20	4705.0
21	6842.5
22	9995.5
23	14354.5
24	20101.0
25	28101.0
26	38664.5
27	51377.0
28	66420.5
29	83601.0
30	100769.5
31	115216.0
32	126988.0
33	138303.0
34	149949.0
35	159709.0
36	167464.5
37	175145.5
38	181365.0
39	184666.0
40	186184.0
41	186324.5
42	184284.5
43	181364.0
44	177150.0
45	172194.5
46	167429.5
47	162142.0
48	155912.5
49	150210.0
50	145568.5
51	138540.5
52	129597.5
53	121762.0
54	115311.5
55	110288.5
56	102617.5
57	92110.0
58	83279.0
59	75558.5
60	66793.0
61	56938.5
62	48283.0
63	40336.0
64	32263.5
65	25756.5
66	20708.5
67	16069.5
68	12575.5
69	9899.5
70	7481.0
71	5496.5
72	4078.5
73	2934.5
74	2149.5
75	1578.0
76	1067.0
77	765.5
78	570.5
79	380.0
80	239.5
81	172.5
82	140.5
83	107.0
84	84.5
85	63.5
86	41.0
87	35.5
88	29.5
89	23.5
90	17.0
91	11.0
92	9.5
93	9.5
94	10.0
95	10.5
96	11.0
97	11.5
98	13.0
99	14.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014204401737777619
2	0.03453891539870228
3	0.036106160841836966
4	0.03860581813088722
5	0.04098644412045888
6	0.04001435517471712
7	0.03761389063523235
8	0.0372766352867097
9	0.0372766352867097
10-11	0.03746510151088413
12-13	0.03857606030601757
14-15	0.03704849196270908
16-17	0.03400327455104866
18-19	0.03646325474027271
20-21	0.03606648374201077
22-23	0.03689970283836085
24-25	0.03414214440044033
26-27	0.03408262875070105
28-29	0.034618269598354665
30-31	0.03465794669818086
32-33	0.034578592498528474
34-35	0.034390126274354046
36-37	0.03218804723400026
38-39	0.03257489895730565
40-41	0.033666019202526
42-43	0.033507310803221224
44-45	0.032505464032609815
46-47	0.03166232566130318
48-49	0.0319103075352169
50-51	0.03280304228130627
52-53	0.033209732554524765
54-55	0.03252530258252291
56-57	0.03127547393799779
58-59	0.032168208684087166
60-61	0.03150361726199841
62-63	0.031622648561476986
64-65	0.02896428287312196
66-67	0.029995887468603016
68-69	0.030749752365300708
70-71	0.031999581009825834
72-73	0.029311457496601162
74-75	0.029628874295210713
76-77	0.030214111517647087
78-79	0.03069023671556142
80-81	0.03069023671556142
82-83	0.029400730971210095
84-85	0.03059104396599593
86-87	0.0308291065649531
88-89	0.031364747412606725
90-91	0.0319103075352169
92-93	0.03024386934251673
94-95	0.03143418233730256
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5040691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.11196384071047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58832578756557	50.51928263141233
2	13.846001072689312	18.030806423667702
3	4.113935120602696	8.03599184447135
4	1.6825819162571882	4.382248515614855
5	0.8438102470594977	2.747107114747949
6	0.49145151584437907	1.9199623997472937
7	0.30289040280746876	1.3805252268708699
8	0.21252997983092162	1.1070595489454316
9	0.1490835481701332	0.8736410337928678
>10	0.7227556179747814	8.124362706800142
>50	0.034533046007955724	1.5318438690437512
>100	0.011949391978780225	1.2467979605254391
>500	9.141193724028309E-5	0.047358163688213537
>1k	6.0941274142203545E-5	0.053012560671788375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.9838549913097232E-5	0.0
6	0.0	0.0	0.0	1.9838549913097232E-5	0.0
7	0.0	0.0	0.0	1.9838549913097232E-5	0.0
8	0.0	0.0	0.0	3.9677099826194463E-5	0.0
9	0.0	0.0	0.0	3.9677099826194463E-5	0.0
10-11	0.0	0.0	0.0	5.9515649739291695E-5	0.0
12-13	0.0	0.0	0.0	7.935419965238893E-5	0.0
14-15	0.0	0.0	0.0	7.935419965238893E-5	0.0
16-17	0.0	0.0	0.0	7.935419965238893E-5	0.0
18-19	0.0	0.0	0.0	8.927347460893754E-5	0.0
20-21	0.0	0.0	0.0	1.3886984939168062E-4	0.0
22-23	0.0	0.0	0.0	1.884662241744237E-4	0.0
24-25	0.0	0.0	0.0	4.8604447287088214E-4	0.0
26-27	0.0	0.0	0.0	9.02654021045924E-4	0.0
28-29	0.0	0.0	0.0	0.002430222364354411	0.0
30-31	0.0	0.0	0.0	0.0068244611701054475	0.0
32-33	0.0	0.0	0.0	0.01592043630526053	0.0
34-35	0.0	0.0	0.0	0.03095805713938823	0.0
36-37	0.0	0.0	0.0	0.054843671234757294	0.0
38-39	0.0	0.0	0.0	0.0853851188259705	0.0
40-41	0.0	0.0	0.0	0.11714663723683916	0.0
42-43	0.0	0.0	0.0	0.14852130392440244	0.0
44-45	0.0	0.0	0.0	0.18836703142485822	0.0
46-47	0.0	1.9838549913097232E-5	0.0	0.2343329515735045	0.0
48-49	0.0	3.9677099826194463E-5	0.0	0.2836615059324208	0.0
50-51	0.0	3.9677099826194463E-5	0.0	0.3324940965435096	0.0
52-53	0.0	3.9677099826194463E-5	0.0	0.38073153065720555	0.0
54-55	0.0	3.9677099826194463E-5	0.0	0.4348709333700479	0.0
56-57	0.0	3.9677099826194463E-5	0.0	0.4994156555123097	0.0
58-59	0.0	5.9515649739291695E-5	0.0	0.5697333956792829	0.0
60-61	0.0	5.9515649739291695E-5	0.0	0.6393667058742541	0.0
62-63	0.0	5.9515649739291695E-5	0.0	0.7070955152775681	0.0
64-65	0.0	5.9515649739291695E-5	0.0	0.7803394415567231	0.0
66-67	0.0	5.9515649739291695E-5	0.0	0.8653377086593882	0.0
68-69	0.0	5.9515649739291695E-5	0.0	0.9600568652194709	0.0
70-71	0.0	5.9515649739291695E-5	0.0	1.059448000284088	0.0
72-73	0.0	5.9515649739291695E-5	0.0	1.1597616279196643	0.0
74-75	0.0	5.9515649739291695E-5	0.0	1.2618706443223755	0.0
76-77	0.0	5.9515649739291695E-5	0.0	1.3725895913873711	0.0
78-79	0.0	5.9515649739291695E-5	0.0	1.496868187317969	0.0
80-81	0.0	5.9515649739291695E-5	0.0	1.6297467946358941	0.0
82-83	0.0	5.9515649739291695E-5	0.0	1.7668410938103527	0.0
84-85	0.0	5.9515649739291695E-5	0.0	1.9000371179268876	0.0
86-87	0.0	5.9515649739291695E-5	0.0	2.0442832143450174	0.0
88	0.0	5.9515649739291695E-5	0.0	2.1618067840302055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4615	0.0	41.252796	1
GTATCAA	7715	0.0	35.03499	1
TCAACGC	7905	0.0	33.121204	4
ATCAACG	7960	0.0	32.892353	3
CAACGCA	8410	0.0	31.243834	5
AACGCAG	8940	0.0	29.441229	6
TATCAAC	9375	0.0	28.078222	2
ACGCAGA	10680	0.0	24.380333	7
CGCAGAG	10895	0.0	23.5541	8
GTACATG	12180	0.0	22.732021	1
TACATGG	12330	0.0	20.853409	2
GCAGAGT	12635	0.0	19.454834	9
ACATGGG	12930	0.0	19.195005	3
CCGGTCG	500	0.0	18.80014	9
GAGTACA	9660	0.0	18.053726	1
AGAGTAC	10880	0.0	17.581932	10-11
GTATAGA	2400	0.0	17.236198	1
CATGGGG	9615	0.0	16.768633	4
CAGAGTA	12275	0.0	16.521915	10-11
AGTACAT	10360	0.0	15.698898	2
>>END_MODULE