FastQCFastQC Report
Wed 25 May 2016
SRR2049387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5040691
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108390.2150300425080609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102720.2037815847073348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60800.12061838347163117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76100.056.7711221
GTATCAA139300.041.7460331
TCAACGC173850.032.9524544
ATCAACG173900.032.9159553
CAACGCA178650.032.067085
TATCAAC180400.031.8862632
AACGCAG184800.031.0248416
ACGCAGA211000.026.9275387
CGCAGAG214000.026.4841128
GCAGAGT235150.023.602029
GTGGTAT31200.023.5053461
GTACATG123850.022.5089551
GAGTACT168000.021.59057412-13
AGAGTAC216650.021.11266510-11
CAGAGTA232350.020.64760410-11
TACATGG129500.020.1773342
ACATGGG128200.019.5388013
TGGTATC35000.019.4696752
AGTACTT179300.019.35145612-13
GTACTTT189300.019.09125714-15
Produced by FastQC (version 0.11.5)