##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049387_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5040691 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85052148604229 31.0 31.0 34.0 30.0 34.0 2 31.909241411544567 33.0 31.0 34.0 30.0 34.0 3 31.91132902215192 33.0 31.0 34.0 30.0 34.0 4 35.6594447070848 37.0 35.0 37.0 33.0 37.0 5 35.752151044370706 37.0 35.0 37.0 35.0 37.0 6 35.82028872628773 37.0 35.0 37.0 35.0 37.0 7 35.78791320475705 37.0 35.0 37.0 35.0 37.0 8 35.800112722640606 37.0 35.0 37.0 35.0 37.0 9 37.52554977085483 39.0 37.0 39.0 35.0 39.0 10-11 37.49395102774599 39.0 37.0 39.0 35.0 39.0 12-13 37.447839492640995 39.0 37.0 39.0 34.5 39.0 14-15 38.7559187817702 40.0 38.0 41.0 35.0 41.0 16-17 38.7930711285417 40.0 38.0 41.0 35.0 41.0 18-19 38.85929052187488 40.0 38.0 41.0 35.0 41.0 20-21 38.88922233082726 40.0 38.0 41.0 35.0 41.0 22-23 38.85907319056058 40.0 38.0 41.0 35.0 41.0 24-25 38.84575428249818 40.0 38.0 41.0 35.0 41.0 26-27 38.7395159711238 40.0 38.0 41.0 35.0 41.0 28-29 38.660187363200805 40.0 38.0 41.0 34.0 41.0 30-31 38.56619519030228 40.0 38.0 41.0 34.0 41.0 32-33 38.44092952732076 40.0 38.0 41.0 34.0 41.0 34-35 38.345968737222734 40.0 38.0 41.0 34.0 41.0 36-37 38.23923555322078 40.0 38.0 41.0 34.0 41.0 38-39 38.15497468501838 40.0 38.0 41.0 34.0 41.0 40-41 38.047604485178724 40.0 38.0 41.0 33.0 41.0 42-43 37.91952879079476 40.0 38.0 41.0 33.0 41.0 44-45 37.760103723874366 40.0 37.0 41.0 33.0 41.0 46-47 37.55190488764338 40.0 37.0 41.0 33.0 41.0 48-49 37.361317724097745 40.0 37.0 41.0 32.0 41.0 50-51 37.226644025590936 40.0 37.0 41.0 32.0 41.0 52-53 37.093341369268614 40.0 37.0 41.0 31.5 41.0 54-55 36.959210949451176 40.0 36.0 41.0 31.0 41.0 56-57 36.824526141356415 39.5 36.0 41.0 31.0 41.0 58-59 36.642803635453944 39.0 35.5 41.0 31.0 41.0 60-61 36.474284676446146 39.0 35.0 41.0 31.0 41.0 62-63 36.28158550087676 39.0 35.0 41.0 31.0 41.0 64-65 36.06452686744734 38.5 35.0 40.5 30.5 41.0 66-67 35.80586491018791 38.0 35.0 40.0 30.0 41.0 68-69 35.526263363495204 37.0 35.0 40.0 30.0 41.0 70-71 35.203411595751454 37.0 35.0 40.0 29.0 41.0 72-73 34.91027956286152 36.5 34.0 39.0 29.0 41.0 74-75 34.60535053626576 36.0 34.0 39.0 29.0 41.0 76-77 34.278713374813094 35.5 34.0 39.0 29.0 40.0 78-79 33.94033159342638 35.0 34.0 37.5 29.0 39.5 80-81 33.61868878691433 35.0 34.0 37.0 29.0 39.0 82-83 33.31671451394263 35.0 34.0 36.5 29.0 39.0 84-85 33.04008329413567 35.0 34.0 36.0 29.0 37.5 86-87 32.797541448186365 35.0 34.0 36.0 28.5 37.0 88-89 32.568015178871306 35.0 33.0 35.5 28.0 37.0 90-91 32.368910433113236 35.0 33.0 35.0 27.0 36.0 92-93 32.185296519862064 35.0 33.0 35.0 27.0 36.0 94-95 32.02866571269693 35.0 33.0 35.0 27.0 36.0 96-97 31.83465312593055 35.0 33.0 35.0 26.5 36.0 98-99 31.68886468144943 35.0 33.0 35.0 26.0 35.5 100 31.57572166197055 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10310267735864187 1101 2 0.09618992725805597 1101 3 0.2845914173917734 1101 4 0.1178848608747387 1101 5 0.10995772681506111 1101 6 0.13549433163388613 1101 7 0.046356522763375096 1101 8 0.045705426310114206 1101 9 0.125645408062951 1101 10-11 0.1536930054882788 1101 12-13 0.08054320273111415 1101 14-15 0.14879573463991136 1101 16-17 0.08375760544450372 1101 18-19 0.20394237154420836 1101 20-21 0.1873300851358266 1101 22-23 0.13852130636578863 1101 24-25 0.133985556871572 1101 26-27 0.22552926379468374 1101 28-29 0.2449880409742775 1101 30-31 0.18191325859801566 1101 32-33 0.20968105644889334 1101 34-35 0.24933504727037104 1101 36-37 0.24581544925585774 1101 38-39 0.19490688174112591 1101 40-41 0.227649049268706 1101 42-43 0.2210184748289592 1101 44-45 0.14322825299227304 1101 46-47 0.1232007710642975 1101 48-49 0.18579971303436338 1101 50-51 0.10985912282935573 1101 52-53 0.14328241603809033 1101 54-55 0.1232208713691989 1101 56-57 0.05117104357457691 1101 58-59 0.08168752008126745 1101 60-61 0.11146335746128955 1101 62-63 0.08957909731084612 1101 64-65 0.06657267304832715 1101 66-67 0.05516570912861596 1101 68-69 0.11597391160071169 1101 70-71 0.12692716498845869 1101 72-73 0.05482592798919228 1101 74-75 0.0756244181163126 1101 76-77 0.06791097345254116 1101 78-79 0.14333158992606343 1101 80-81 0.16398979301084182 1101 82-83 0.14802421907240415 1101 84-85 0.17342147623296 1101 86-87 0.08902354829236003 1101 88-89 0.19048176491554614 1101 90-91 0.257558827701466 1101 92-93 0.23787584907464776 1101 94-95 0.1921225766799992 1101 96-97 0.21273601529621544 1101 98-99 0.29226937212383675 1101 100 0.29349646385872674 1102 1 0.12482730250073004 1102 2 0.12081759827903582 1102 3 0.30222270591510636 1102 4 0.1522416662475834 1102 5 0.1462118524308309 1102 6 0.12275388674662224 1102 7 0.13712291826387002 1102 8 0.15771467111149207 1102 9 0.11411544777472216 1102 10-11 0.15649411374381827 1102 12-13 0.1898185769309393 1102 14-15 0.2538291856548014 1102 16-17 0.19250550438533054 1102 18-19 0.17047160464667854 1102 20-21 0.21533280999064175 1102 22-23 0.22039202486574538 1102 24-25 0.20090747028297073 1102 26-27 0.25882578814760393 1102 28-29 0.24189366110014987 1102 30-31 0.1621813668630523 1102 32-33 0.19404643105014685 1102 34-35 0.2127467264471008 1102 36-37 0.214274747113123 1102 38-39 0.20647444698125383 1102 40-41 0.26258134202288375 1102 42-43 0.2814489274318177 1102 44-45 0.2747992083188109 1102 46-47 0.23022389068274407 1102 48-49 0.2097637694692409 1102 50-51 0.24761445692726625 1102 52-53 0.32286931059953616 1102 54-55 0.21270213644114477 1102 56-57 0.30322962795307973 1102 58-59 0.31071054249090935 1102 60-61 0.29668405669340103 1102 62-63 0.3229173537591308 1102 64-65 0.2699124064310965 1102 66-67 0.2598316093436921 1102 68-69 0.3106052876495724 1102 70-71 0.34301288998890556 1102 72-73 0.33316606797550463 1102 74-75 0.2682330708089111 1102 76-77 0.2686509354922251 1102 78-79 0.29945805237089473 1102 80-81 0.3067651625570562 1102 82-83 0.2924577225650111 1102 84-85 0.3213069920934615 1102 86-87 0.3060918624821056 1102 88-89 0.30776691865916206 1102 90-91 0.31777351249159125 1102 92-93 0.3817582990034154 1102 94-95 0.3583421236023767 1102 96-97 0.43907449539995014 1102 98-99 0.40677045265512035 1102 100 0.44134963946059713 1103 1 0.11619206271227256 1103 2 0.07738599715804995 1103 3 0.25789289853515385 1103 4 0.16044863544200183 1103 5 0.12253852656849062 1103 6 0.11680425217933532 1103 7 0.11042889286127888 1103 8 0.13047145229182178 1103 9 0.17480531130370025 1103 10-11 0.1725350574406619 1103 12-13 0.1698573790214155 1103 14-15 0.18764200335097314 1103 16-17 0.15399401058140683 1103 18-19 0.14469892572596876 1103 20-21 0.15601275354044475 1103 22-23 0.1899572018872746 1103 24-25 0.15806691769481063 1103 26-27 0.14076392742680355 1103 28-29 0.24689564154847687 1103 30-31 0.23286053973573928 1103 32-33 0.2532354199022322 1103 34-35 0.28013948088041474 1103 36-37 0.29109878491242824 1103 38-39 0.33525267434280437 1103 40-41 0.31580033435947996 1103 42-43 0.3817623548280622 1103 44-45 0.3676650223924014 1103 46-47 0.3090385396872719 1103 48-49 0.3346166023574142 1103 50-51 0.31775146804231724 1103 52-53 0.31696334109894764 1103 54-55 0.3413971241584548 1103 56-57 0.3053896596170844 1103 58-59 0.3470829005145575 1103 60-61 0.2898151932868558 1103 62-63 0.2864346973245233 1103 64-65 0.31100278169837026 1103 66-67 0.26077159832036045 1103 68-69 0.29292527047096684 1103 70-71 0.315682386284621 1103 72-73 0.29371058520330706 1103 74-75 0.2844300051284705 1103 76-77 0.22098692696779665 1103 78-79 0.24455605904294941 1103 80-81 0.3528188305710671 1103 82-83 0.3394592980911142 1103 84-85 0.34203178115996735 1103 86-87 0.300569421588051 1103 88-89 0.342037009603402 1103 90-91 0.3280570746111735 1103 92-93 0.3501169174721994 1103 94-95 0.2791108510381761 1103 96-97 0.3192160773651409 1103 98-99 0.3491732432664527 1103 100 0.36542245045704647 1104 1 0.1304458255171923 1104 2 0.11473195062621855 1104 3 0.2043482303790043 1104 4 0.1382553777085036 1104 5 0.10299267724366956 1104 6 0.10389036534050433 1104 7 0.16182877236415294 1104 8 0.12081547536969595 1104 9 0.15199654651686245 1104 10-11 0.1626826741220242 1104 12-13 0.11608110813446615 1104 14-15 0.16819288706424373 1104 16-17 0.18525418356465906 1104 18-19 0.22625670813078358 1104 20-21 0.22659122235883444 1104 22-23 0.21040599207847777 1104 24-25 0.271655289512033 1104 26-27 0.280688783340608 1104 28-29 0.2746432693750478 1104 30-31 0.25965762150983096 1104 32-33 0.291066333701977 1104 34-35 0.25132908477586824 1104 36-37 0.3073724777685243 1104 38-39 0.2953048874308948 1104 40-41 0.2300723376515208 1104 42-43 0.2496745026774505 1104 44-45 0.20982694751252495 1104 46-47 0.2156257357964222 1104 48-49 0.17556492776797938 1104 50-51 0.17651601685226836 1104 52-53 0.16710790334756354 1104 54-55 0.15472469306381242 1104 56-57 0.1522968066816901 1104 58-59 0.24674016417686317 1104 60-61 0.2093822934267493 1104 62-63 0.26356565597116344 1104 64-65 0.19523480993552056 1104 66-67 0.23399415368554344 1104 68-69 0.2590015348192054 1104 70-71 0.31889767905862243 1104 72-73 0.2636840341108311 1104 74-75 0.2753709657367196 1104 76-77 0.2034313244604533 1104 78-79 0.22670248657476577 1104 80-81 0.2661422655260708 1104 82-83 0.30590651535096214 1104 84-85 0.3118163844946551 1104 86-87 0.24786342729703392 1104 88-89 0.22847923111901736 1104 90-91 0.25634000175325156 1104 92-93 0.3321007347892575 1104 94-95 0.362564247451175 1104 96-97 0.4069411466341819 1104 98-99 0.38332522128294144 1104 100 0.4095024725346512 1105 1 0.1315257563132164 1105 2 0.037415211648191615 1105 3 0.19006190779580834 1105 4 0.09427212413817898 1105 5 0.11143630251417136 1105 6 0.10101793393998548 1105 7 0.12151993973264297 1105 8 0.09739521125707284 1105 9 0.08708517292538431 1105 10-11 0.14638232926804307 1105 12-13 0.11689604933997799 1105 14-15 0.2003554251652062 1105 16-17 0.18527377581271054 1105 18-19 0.12471730997017261 1105 20-21 0.14664111289063442 1105 22-23 0.1965433740370841 1105 24-25 0.17738205546299213 1105 26-27 0.18538410487397528 1105 28-29 0.1710142933272749 1105 30-31 0.13796930705652244 1105 32-33 0.1576085781878831 1105 34-35 0.21385522255828704 1105 36-37 0.1494999931419727 1105 38-39 0.1755130924514816 1105 40-41 0.1524436434482368 1105 42-43 0.1370864452805236 1105 44-45 0.12320627320462307 1105 46-47 0.14616361270640965 1105 48-49 0.11493606983130178 1105 50-51 0.1623658261763481 1105 52-53 0.21146591885813848 1105 54-55 0.1620502168713145 1105 56-57 0.1125174209708959 1105 58-59 0.11960806883028141 1105 60-61 0.18225549533740804 1105 62-63 0.1549814379451817 1105 64-65 0.08936707104316355 1105 66-67 0.20656148334577296 1105 68-69 0.18473056667828303 1105 70-71 0.19429521413583473 1105 72-73 0.2495074585316459 1105 74-75 0.2414632475061964 1105 76-77 0.20378312317075853 1105 78-79 0.22858848757136485 1105 80-81 0.20438684065310042 1105 82-83 0.18634379506490006 1105 84-85 0.18937892634678377 1105 86-87 0.16213834430244134 1105 88-89 0.13729232107252898 1105 90-91 0.16120972724380778 1105 92-93 0.19316335952192532 1105 94-95 0.18189948982477233 1105 96-97 0.19729605779993165 1105 98-99 0.23495490092814109 1105 100 0.29012546040063825 1106 1 0.13072195849673918 1106 2 0.13361487244518244 1106 3 0.19651941995141442 1106 4 0.12558202591322498 1106 5 0.16454080408797722 1106 6 0.11332460952303336 1106 7 0.11330214509810332 1106 8 0.123285391698019 1106 9 0.1475217861723337 1106 10-11 0.14650358545233644 1106 12-13 0.13305240199365898 1106 14-15 0.18880225833994047 1106 16-17 0.1953892380026616 1106 18-19 0.22345731264177715 1106 20-21 0.1869309201338325 1106 22-23 0.17557614505106045 1106 24-25 0.19665045718489438 1106 26-27 0.22100993277208403 1106 28-29 0.1990571432446231 1106 30-31 0.18139696959543983 1106 32-33 0.10012450340111201 1106 34-35 0.10598047705359903 1106 36-37 0.1583279148184289 1106 38-39 0.17014899511733717 1106 40-41 0.15536987940731706 1106 42-43 0.15541225800092917 1106 44-45 0.13740569496316368 1106 46-47 0.18205713885858898 1106 48-49 0.19058764605830447 1106 50-51 0.14956415902788223 1106 52-53 0.10276343033549296 1106 54-55 0.17540651113012729 1106 56-57 0.1487705800625676 1106 58-59 0.19023334462399788 1106 60-61 0.16078769170790963 1106 62-63 0.19501334833801565 1106 64-65 0.08875632365924702 1106 66-67 0.09657331533565383 1106 68-69 0.16901105886589818 1106 70-71 0.2321789215147234 1106 72-73 0.2587621755088776 1106 74-75 0.21565937731647722 1106 76-77 0.2245509742357399 1106 78-79 0.19492070718401067 1106 80-81 0.18521914111698834 1106 82-83 0.20884339287650278 1106 84-85 0.18671100079941283 1106 86-87 0.1979869163371788 1106 88-89 0.20816664350397218 1106 90-91 0.2110994924691667 1106 92-93 0.23434579755699048 1106 94-95 0.20675092825313612 1106 96-97 0.22341890784962715 1106 98-99 0.23408348583290461 1106 100 0.26482493571536736 1107 1 0.11758316890419707 1107 2 0.10970535001725423 1107 3 0.19605613948984058 1107 4 0.14154417051069856 1107 5 0.1275569410529087 1107 6 0.11466775659951622 1107 7 0.11221899482053033 1107 8 0.13788694660370027 1107 9 0.14174492994424526 1107 10-11 0.13659577755794317 1107 12-13 0.12970418358353442 1107 14-15 0.19735694960277783 1107 16-17 0.14494341247463893 1107 18-19 0.17449346886750305 1107 20-21 0.13116081840249194 1107 22-23 0.14574239107751197 1107 24-25 0.09694160268430352 1107 26-27 0.17987756869739968 1107 28-29 0.15297990448617327 1107 30-31 0.16184327224961237 1107 32-33 0.17246876872660977 1107 34-35 0.1095589232039984 1107 36-37 0.07380344001236949 1107 38-39 0.1233929807615084 1107 40-41 0.14557943961936104 1107 42-43 0.12023402592496524 1107 44-45 0.08644771844450361 1107 46-47 0.2572084991238981 1107 48-49 0.22393791011601394 1107 50-51 0.20857598395493682 1107 52-53 0.1948238458392879 1107 54-55 0.1819976024375407 1107 56-57 0.19156139687051876 1107 58-59 0.23022728241183898 1107 60-61 0.18158631876713827 1107 62-63 0.2244529599753946 1107 64-65 0.17894478684740278 1107 66-67 0.17235191171920405 1107 68-69 0.15591709121045483 1107 70-71 0.15069521849348178 1107 72-73 0.22828362783161538 1107 74-75 0.15850542907292464 1107 76-77 0.14513822866051385 1107 78-79 0.14875235457579095 1107 80-81 0.2268768449017955 1107 82-83 0.17561987520522138 1107 84-85 0.20220969248007492 1107 86-87 0.16287553940776434 1107 88-89 0.21793898370428622 1107 90-91 0.22905779821308414 1107 92-93 0.26103881319384215 1107 94-95 0.27946102861962885 1107 96-97 0.27868070187845717 1107 98-99 0.3539507314345407 1107 100 0.38703707979958324 1108 1 0.1267053398359259 1108 2 0.08892084168478576 1108 3 0.1547362179655849 1108 4 0.08593090537502235 1108 5 0.05380171444186743 1108 6 0.04854310093559633 1108 7 0.04696927359569969 1108 8 -0.006950386026119304 1108 9 0.06581211230749773 1108 10-11 0.12032087067602504 1108 12-13 0.05852721410407469 1108 14-15 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-0.25990788306093293 2215 7 -0.23034626052507434 2215 8 -0.19894301156755745 2215 9 -0.3926696667048759 2215 10-11 -0.3446509026383211 2215 12-13 -0.3547077847486193 2215 14-15 -0.3790982592174217 2215 16-17 -0.48432991865733044 2215 18-19 -0.38817230972744454 2215 20-21 -0.3906152794009756 2215 22-23 -0.3738710220039607 2215 24-25 -0.40026868324439846 2215 26-27 -0.4078286895624075 2215 28-29 -0.34825557223264525 2215 30-31 -0.32637800955990315 2215 32-33 -0.31180849740834304 2215 34-35 -0.3400237889699227 2215 36-37 -0.3602585458426688 2215 38-39 -0.40943261643183604 2215 40-41 -0.39694320603426547 2215 42-43 -0.3699168004287188 2215 44-45 -0.40635500118263934 2215 46-47 -0.39380558058780935 2215 48-49 -0.32887676572268276 2215 50-51 -0.3868326764798198 2215 52-53 -0.3903740434003069 2215 54-55 -0.28992466776097814 2215 56-57 -0.33783138352785613 2215 58-59 -0.3075367599338463 2215 60-61 -0.34643545159671163 2215 62-63 -0.318556881245442 2215 64-65 -0.34894969276131604 2215 66-67 -0.3088959122181194 2215 68-69 -0.3735928616471327 2215 70-71 -0.4098571172445986 2215 72-73 -0.4266478870124004 2215 74-75 -0.34321237087618073 2215 76-77 -0.2717199625300779 2215 78-79 -0.34138208578094975 2215 80-81 -0.41489515129372023 2215 82-83 -0.37225371725175194 2215 84-85 -0.36188794317382644 2215 86-87 -0.3420018299719345 2215 88-89 -0.4068784771286502 2215 90-91 -0.3668707916580374 2215 92-93 -0.33408632908182767 2215 94-95 -0.3857465726590661 2215 96-97 -0.4587950094457547 2215 98-99 -0.34712055591754876 2215 100 -0.32842616056859697 2216 1 -0.3113824745419791 2216 2 -0.24621754764280723 2216 3 -0.4122571224823339 2216 4 -0.1878209203394121 2216 5 -0.39563162345252323 2216 6 -0.30472523019708575 2216 7 -0.5222577512274498 2216 8 -0.3313543701010815 2216 9 -0.535693363715005 2216 10-11 -0.4300244420044308 2216 12-13 -0.3656499996790572 2216 14-15 -0.6750180334644185 2216 16-17 -0.47489852402261334 2216 18-19 -0.3968558708428631 2216 20-21 -0.40142662328118917 2216 22-23 -0.42805084041970787 2216 24-25 -0.43022760176874897 2216 26-27 -0.49714525392037956 2216 28-29 -0.5174485314350363 2216 30-31 -0.646081650488675 2216 32-33 -0.5659359423329278 2216 34-35 -0.5826845540414283 2216 36-37 -0.6119070506239552 2216 38-39 -0.5561829749925593 2216 40-41 -0.5418322431932125 2216 42-43 -0.5207815816988628 2216 44-45 -0.65026436514934 2216 46-47 -0.6567524792683699 2216 48-49 -0.734711439277433 2216 50-51 -0.558864456465912 2216 52-53 -0.4892329714280734 2216 54-55 -0.4386272303972447 2216 56-57 -0.5846265544362481 2216 58-59 -0.5991012373601663 2216 60-61 -0.44050418063617514 2216 62-63 -0.3297733369482927 2216 64-65 -0.4945758713602757 2216 66-67 -0.4243137371442174 2216 68-69 -0.3460013095754704 2216 70-71 -0.4340221086222158 2216 72-73 -0.44564676801625325 2216 74-75 -0.4072413787674378 2216 76-77 -0.3632694386004971 2216 78-79 -0.5330852195659688 2216 80-81 -0.6846862268850487 2216 82-83 -0.5328078377254357 2216 84-85 -0.6714689354825509 2216 86-87 -0.5063657521298239 2216 88-89 -0.8011729830634486 2216 90-91 -0.6550135751298356 2216 92-93 -0.6538609801646231 2216 94-95 -0.6281909669154615 2216 96-97 -0.6745748696505771 2216 98-99 -0.7941577542069815 2216 100 -0.7612181541744647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 266.0 3 27.0 4 37.0 5 86.0 6 192.0 7 555.0 8 1448.0 9 2700.0 10 3766.0 11 5098.0 12 7071.0 13 10097.0 14 12376.0 15 15934.0 16 19360.0 17 26368.0 18 25883.0 19 14337.0 20 11162.0 21 11176.0 22 11867.0 23 13198.0 24 15689.0 25 19797.0 26 25767.0 27 33888.0 28 44412.0 29 58484.0 30 76869.0 31 99825.0 32 129193.0 33 168847.0 34 230968.0 35 334575.0 36 542449.0 37 992467.0 38 1447744.0 39 623578.0 40 3135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.12409360573551 20.849995296613045 14.631462840663142 23.3944482569883 2 15.187818495519762 20.787626140939803 39.91821756183825 24.10633780170219 3 17.790259311669768 25.450101980065824 30.905187403869828 25.854451304394576 4 12.418381693455002 17.29156470474572 38.77430248126794 31.515751120531338 5 12.43873111841214 37.96983389777314 36.09088515840388 13.50054982541084 6 30.481118560927463 38.71211308132159 17.25275364032431 13.554014717426638 7 26.224460098823755 33.094292032580455 22.683794741633637 17.997453126962156 8 24.599605527377996 36.767054433809584 20.333710665322396 18.299629373490028 9 24.4106611573691 16.60335854746899 21.879539928156674 37.10644036700524 10-11 23.52549481807157 27.249756035432444 28.99847461389718 20.226274532598804 12-13 24.077177420083107 24.66686057479569 29.470889815030105 21.785072190091096 14-15 21.290529605861906 26.773636308178506 26.980572571273832 24.95526151468576 16-17 20.422011585316376 30.26316233230722 27.653956570636844 21.660869511739563 18-19 20.303553740371605 28.591498106707235 30.56910473945129 20.535843413469866 20-21 21.343413702314674 27.7311621219998 30.402077017851823 20.523347157833697 22-23 20.900021445472454 27.624367373441462 30.532688871426554 20.94292230965953 24-25 20.897939733768247 27.75564067849132 30.407671896083137 20.93874769165729 26-27 20.700198346018777 27.776824424294627 30.46741058657492 21.05556664311167 28-29 20.6702557376763 27.73765526944517 30.651502289476028 20.940586703402502 30-31 20.966869519166075 28.048825850347285 30.23377451958219 20.75053011090445 32-33 20.662031644338498 27.847499955611156 30.584630388694546 20.905838011355808 34-35 20.841210481464984 27.653504079849412 30.546372782210206 20.958912656475402 36-37 21.105877255595768 28.000018251484025 29.99520303387277 20.89890145904744 38-39 20.969050483600764 28.07972302924022 30.108151157885253 20.84307532927376 40-41 20.876176377580354 27.860553107255637 30.202744229654456 21.06052628550955 42-43 20.887993328662656 27.902618472133213 30.071070930373622 21.138317268830516 44-45 21.01699205771604 27.960977872324133 29.853689339639494 21.168340730320335 46-47 21.134619712858445 28.23466733535892 29.44057498540129 21.19013796638135 48-49 21.138146865979586 28.20269211312033 29.24864848779423 21.410512533105848 50-51 21.242444577032703 28.213287228328394 29.07255834668722 21.471709847951683 52-53 21.219946661778422 28.231752450024928 29.11560443536892 21.43269645282773 54-55 21.31279809414168 28.564306500219168 28.837792050796434 21.285103354842715 56-57 21.2075505086926 28.32796731067535 28.90595629506968 21.558525885562364 58-59 21.090665256805373 27.981454901466762 29.25316698028145 21.67471286144641 60-61 21.27482166845892 28.34277468235198 28.932079918161858 21.450323731027243 62-63 21.098704870182495 28.308990918438543 29.03849957535264 21.553804636026324 64-65 21.135844698469242 28.142540135773576 29.12692287128808 21.5946922944691 66-67 21.104218226906386 28.193954122752636 28.984371977681338 21.717455672659643 68-69 21.4299261398918 28.243942697411622 28.864958804589126 21.461172358107454 70-71 21.418929856971452 28.217686054557806 28.86580400391059 21.497580084560152 72-73 21.23390744300507 28.324100554184973 28.89494014209093 21.54705186071903 74-75 21.338243551990228 28.32879803366415 28.79736906659456 21.535589347751067 76-77 21.348762537426914 28.307309115923868 28.69491728013657 21.649011066512653 78-79 21.400354446783485 28.384807076873447 28.71723237095457 21.4976061053885 80-81 21.28070370000776 28.28043607085487 28.76659266029528 21.672267568842095 82-83 21.221775198688178 28.33920677884258 28.836942724061277 21.602075298407968 84-85 21.287747015577178 28.22598140380584 28.751827058469342 21.734444522147644 86-87 21.35861253383269 28.379653427073254 28.630138834304482 21.631595204789576 88-89 21.225309588916627 28.246047515615963 28.69471474154195 21.833928153925463 90-91 21.15807588873477 28.211839163202512 28.700000128954166 21.930084819108547 92-93 21.214861705497132 28.392419461665565 28.605302321733543 21.787416511103764 94-95 21.095746481158947 28.18894877481619 28.733387838410763 21.981916905614096 96-97 21.136159157708455 28.240791900066164 28.67575564895393 21.947293293271446 98-99 21.093937696791794 28.29521541627559 28.631568067170544 21.979278819762076 100 21.21853757556616 28.17344150000714 28.609023182880822 21.998997741545875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 506.0 1 384.5 2 479.0 3 716.0 4 463.0 5 282.5 6 810.5 7 2148.5 8 3624.5 9 4183.0 10 3221.5 11 2073.5 12 2023.0 13 2321.0 14 2700.0 15 3186.0 16 3699.0 17 4300.5 18 5127.5 19 6233.0 20 7744.0 21 9787.0 22 12901.0 23 17093.5 24 22678.5 25 30218.5 26 40367.5 27 52834.5 28 67592.0 29 84592.5 30 101238.0 31 115085.5 32 126353.5 33 137687.0 34 149092.0 35 158606.5 36 165976.0 37 173709.5 38 180734.0 39 183175.5 40 184191.5 41 184876.0 42 183002.5 43 180753.5 44 176927.0 45 171608.5 46 167481.0 47 161702.5 48 154383.0 49 149227.5 50 144591.0 51 137898.5 52 128644.0 53 120397.0 54 114202.5 55 108593.0 56 100835.5 57 90417.0 58 81460.0 59 74250.5 60 65796.0 61 55484.5 62 46899.0 63 39087.5 64 30945.5 65 24716.0 66 19583.0 67 14834.5 68 11465.0 69 8836.5 70 6480.0 71 4667.5 72 3418.5 73 2382.0 74 1713.5 75 1182.0 76 760.0 77 514.5 78 315.5 79 219.0 80 127.0 81 50.5 82 33.0 83 18.5 84 9.0 85 5.5 86 1.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035094394796269 2 0.0 3 0.0 4 3.9677099826194463E-5 5 0.0 6 0.0 7 0.0 8 7.340263467845976E-4 9 0.0 10-11 0.0 12-13 0.0440911771818586 14-15 1.6862767426132648E-4 16-17 0.0 18-19 3.9677099826194463E-5 20-21 4.959637478274308E-5 22-23 0.0 24-25 1.4878912434822922E-4 26-27 9.919274956548616E-5 28-29 3.5709389843575017E-4 30-31 9.919274956548616E-6 32-33 2.0830477408752092E-4 34-35 4.959637478274308E-5 36-37 9.919274956548616E-5 38-39 2.876589737399099E-4 40-41 9.919274956548616E-5 42-43 4.4636737304468773E-4 44-45 2.479818739137154E-4 46-47 1.28950574435132E-4 48-49 8.133805464369865E-4 50-51 4.6620592295778493E-4 52-53 5.554793975667225E-4 54-55 4.4636737304468773E-4 56-57 4.7612519791433356E-4 58-59 1.1903129947858339E-4 60-61 1.8846622417442372E-4 62-63 7.538648966976949E-4 64-65 3.86851723305396E-4 66-67 0.0013093442942644173 68-69 0.001606922542960876 70-71 3.7693244834884743E-4 72-73 9.02654021045924E-4 74-75 9.224925709590212E-4 76-77 6.645914220887573E-4 78-79 0.003174167986095557 80-81 0.002658365688355029 82-83 0.001319263569220966 84-85 0.002846831912529453 86-87 0.001547406893221584 88-89 0.0018945815167007859 90-91 0.0027873162627901613 92-93 0.002489738014093703 94-95 0.003610616084183696 96-97 0.003094813786443168 98-99 0.0029361053871383904 100 0.0014878912434822924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5040691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.84266895643342 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51298801665007 48.08280376797898 2 14.335690832331572 18.01786146475981 3 4.343611346280325 8.188923897291076 4 1.7788539030863446 4.471517078140586 5 0.912923269767304 2.868526741230572 6 0.5431525321766557 2.0479892863455675 7 0.3398643438419992 1.4950587715110752 8 0.22170462667949176 1.1146008368423166 9 0.17510520130142906 0.9903670378141808 >10 0.7801544197790018 8.52158713289961 >50 0.04045865275421556 1.7375503628119995 >100 0.014798084519190138 1.5699247657846742 >500 4.737075737786772E-4 0.2209855100269316 >1k 1.2632186205611794E-4 0.13267055945085116 >5k 3.158046551146178E-5 0.12066372496931281 >10k+ 6.316093102292356E-5 0.41896906214262547 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10839 0.2150300425080609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10272 0.2037815847073348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6080 0.12061838347163117 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.9838549913097232E-5 0.0 6 0.0 0.0 0.0 1.9838549913097232E-5 0.0 7 0.0 0.0 0.0 1.9838549913097232E-5 0.0 8 0.0 0.0 0.0 1.9838549913097232E-5 0.0 9 0.0 0.0 0.0 3.9677099826194463E-5 0.0 10-11 0.0 0.0 0.0 3.9677099826194463E-5 0.0 12-13 0.0 0.0 0.0 3.9677099826194463E-5 0.0 14-15 0.0 0.0 0.0 3.9677099826194463E-5 0.0 16-17 0.0 0.0 0.0 3.9677099826194463E-5 0.0 18-19 0.0 0.0 0.0 3.9677099826194463E-5 0.0 20-21 0.0 0.0 0.0 5.9515649739291695E-5 0.0 22-23 0.0 9.919274956548616E-6 0.0 1.2895057443513202E-4 0.0 24-25 0.0 1.9838549913097232E-5 0.0 3.8685172330539606E-4 0.0 26-27 0.0 1.9838549913097232E-5 0.0 7.836227215673407E-4 0.0 28-29 0.0 1.9838549913097232E-5 0.0 0.002350868164702022 0.0 30-31 0.0 1.9838549913097232E-5 0.0 0.006794703345235803 0.0 32-33 0.0 1.9838549913097232E-5 0.0 0.015950194130130175 0.0 34-35 0.0 1.9838549913097232E-5 0.0 0.031106846263736457 0.0 36-37 0.0 1.9838549913097232E-5 0.0 0.05526028078293234 0.0 38-39 0.0 1.9838549913097232E-5 0.0 0.08613898372266818 0.0 40-41 0.0 1.9838549913097232E-5 0.0 0.11807904908275471 0.0 42-43 0.0 1.9838549913097232E-5 0.0 0.1496818590943186 0.0 44-45 0.0 1.9838549913097232E-5 0.0 0.18978548774364468 0.0 46-47 0.0 1.9838549913097232E-5 0.0 0.23611842106568326 0.0 48-49 0.0 1.9838549913097232E-5 0.0 0.2856850380235567 0.0 50-51 0.0 1.9838549913097232E-5 0.0 0.3344481937099497 0.0 52-53 0.0 1.9838549913097232E-5 0.0 0.38312207592173375 0.0 54-55 0.0 1.9838549913097232E-5 0.0 0.4373705906590981 0.0 56-57 0.0 1.9838549913097232E-5 0.0 0.5021037790255344 0.0 58-59 0.0 1.9838549913097232E-5 0.0 0.5730563527897267 0.0 60-61 0.0 1.9838549913097232E-5 0.0 0.6429376448586117 0.0 62-63 0.0 1.9838549913097232E-5 0.0 0.7111921758346227 0.0 64-65 0.0 1.9838549913097232E-5 0.0 0.7847634381873438 0.0 66-67 0.0 1.9838549913097232E-5 0.0 0.8704362159870542 0.0 68-69 0.0 8.927347460893754E-5 0.0 0.9657406097695733 0.0 70-71 0.0 9.919274956548616E-5 0.0 1.0657070627816703 0.0 72-73 0.0 9.919274956548616E-5 0.0 1.1665563312648999 0.0 74-75 0.0 9.919274956548616E-5 0.0 1.2697763064627448 0.0 76-77 0.0 9.919274956548616E-5 0.0 1.3816359701477436 0.0 78-79 0.0 9.919274956548616E-5 0.0 1.5069759284986919 0.0 80-81 0.0 9.919274956548616E-5 0.0 1.6412531535854906 9.919274956548616E-6 82-83 0.0 9.919274956548616E-5 0.0 1.7795675235796045 1.9838549913097232E-5 84-85 0.0 9.919274956548616E-5 0.0 1.914330793139274 1.9838549913097232E-5 86-87 0.0 9.919274956548616E-5 0.0 2.060094538625756 1.9838549913097232E-5 88 0.0 9.919274956548616E-5 0.0 2.1791258381043392 1.9838549913097232E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7610 0.0 56.771122 1 GTATCAA 13930 0.0 41.746033 1 TCAACGC 17385 0.0 32.952454 4 ATCAACG 17390 0.0 32.915955 3 CAACGCA 17865 0.0 32.06708 5 TATCAAC 18040 0.0 31.886263 2 AACGCAG 18480 0.0 31.024841 6 ACGCAGA 21100 0.0 26.927538 7 CGCAGAG 21400 0.0 26.484112 8 GCAGAGT 23515 0.0 23.60202 9 GTGGTAT 3120 0.0 23.505346 1 GTACATG 12385 0.0 22.508955 1 GAGTACT 16800 0.0 21.590574 12-13 AGAGTAC 21665 0.0 21.112665 10-11 CAGAGTA 23235 0.0 20.647604 10-11 TACATGG 12950 0.0 20.177334 2 ACATGGG 12820 0.0 19.538801 3 TGGTATC 3500 0.0 19.469675 2 AGTACTT 17930 0.0 19.351456 12-13 GTACTTT 18930 0.0 19.091257 14-15 >>END_MODULE