FastQCFastQC Report
Wed 25 May 2016
SRR2049386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6886270
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65400.044.9251861
GTATCAA110150.034.0142941
TCAACGC118150.030.7149014
ATCAACG119900.030.2666023
CAACGCA123600.029.4366265
AACGCAG133850.027.143956
TATCAAC135700.026.8464812
GTACATG181050.025.8092541
TACATGG181200.024.5413532
ACGCAGA154400.023.0441427
CGCAGAG154600.022.6495068
ACATGGG188750.022.638233
GTGGTAT44600.019.8157561
CATGGGG127750.019.6860434
TGGTATC41550.018.8934362
GCAGAGT177500.018.7211699
GAGTACA138150.017.0820671
CAGAGTA169350.016.860610-11
AGAGTAC154000.016.66417710-11
AGTACAT145500.015.0552662
Produced by FastQC (version 0.11.5)