##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049386_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6886270
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.498897225929277	31.0	31.0	34.0	27.0	34.0
2	30.580360630646197	31.0	31.0	34.0	27.0	34.0
3	30.80902970693859	31.0	31.0	34.0	27.0	34.0
4	34.42040015857641	37.0	35.0	37.0	31.0	37.0
5	34.391328977806566	37.0	35.0	37.0	30.0	37.0
6	34.362790451144086	37.0	35.0	37.0	30.0	37.0
7	34.36244846629598	37.0	35.0	37.0	30.0	37.0
8	34.3252704294197	37.0	35.0	37.0	30.0	37.0
9	35.85832286564425	39.0	37.0	39.0	30.0	39.0
10-11	35.8070311649122	39.0	37.0	39.0	30.0	39.0
12-13	35.73209908992822	39.0	37.0	39.0	30.0	39.0
14-15	36.879566804670745	40.0	37.0	41.0	30.0	41.0
16-17	36.844034361127285	40.0	37.0	41.0	30.0	41.0
18-19	36.86336848540647	40.0	37.0	41.0	30.0	41.0
20-21	36.93554493216212	40.0	37.0	41.0	30.0	41.0
22-23	36.88481717969235	40.0	37.0	41.0	30.0	41.0
24-25	36.886297952302186	40.0	37.0	41.0	30.0	41.0
26-27	36.865264795019655	40.0	37.0	41.0	30.0	41.0
28-29	36.81029410696938	40.0	37.0	41.0	30.0	41.0
30-31	36.74561903614003	40.0	37.0	41.0	30.0	41.0
32-33	36.68749119624992	40.0	37.0	41.0	30.0	41.0
34-35	36.5925969356415	40.0	36.5	41.0	30.0	41.0
36-37	36.500437392085985	40.0	36.0	41.0	30.0	41.0
38-39	36.389019382045724	40.0	36.0	41.0	30.0	41.0
40-41	36.2856200816988	40.0	36.0	41.0	30.0	41.0
42-43	36.14550656596387	40.0	36.0	41.0	29.0	41.0
44-45	36.010855731767705	39.5	35.0	41.0	29.0	41.0
46-47	35.888393498947906	39.0	35.0	41.0	28.0	41.0
48-49	35.761627557444015	39.0	35.0	41.0	28.0	41.0
50-51	35.62561611728846	39.0	35.0	41.0	27.5	41.0
52-53	35.473334040053615	39.0	35.0	41.0	27.0	41.0
54-55	35.331478362016014	39.0	35.0	40.0	27.0	41.0
56-57	35.199782393080724	39.0	35.0	40.0	27.0	41.0
58-59	35.004419809272655	38.5	34.0	40.0	26.0	41.0
60-61	34.78407606730494	38.0	34.0	40.0	26.0	41.0
62-63	34.563691955151334	38.0	34.0	40.0	26.0	41.0
64-65	34.31783592568981	37.5	34.0	40.0	26.0	41.0
66-67	34.056789887704085	37.0	34.0	40.0	25.0	41.0
68-69	33.7634837873043	37.0	33.0	40.0	24.5	41.0
70-71	33.473552518271866	36.0	33.0	39.0	24.0	41.0
72-73	33.12769765054231	36.0	33.0	39.0	23.5	41.0
74-75	32.77966722187774	35.0	32.5	39.0	22.0	40.0
76-77	32.444085041684396	35.0	32.0	37.5	22.0	40.0
78-79	32.114802425696354	35.0	32.0	37.0	21.5	39.0
80-81	31.75469201759443	35.0	32.0	37.0	20.0	39.0
82-83	31.425352694564694	35.0	32.0	36.0	19.5	39.0
84-85	31.12091814581769	35.0	32.0	36.0	18.0	37.0
86-87	30.78901589684982	35.0	31.0	35.5	15.5	37.0
88-89	30.515685487208604	35.0	31.0	35.0	11.0	36.5
90-91	30.286052609032176	35.0	31.0	35.0	7.0	36.0
92-93	30.02152682076073	34.0	31.0	35.0	2.0	36.0
94-95	29.784163850676784	34.0	31.0	35.0	2.0	36.0
96-97	29.49392864351819	34.0	31.0	35.0	2.0	35.5
98-99	29.254632551439315	34.0	31.0	35.0	2.0	35.0
100	29.105217047835765	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5252116494494636
1101	2	0.5129649816146191
1101	3	0.5138831227786653
1101	4	0.5877295256148685
1101	5	0.5661901016137918
1101	6	0.6169470374565833
1101	7	0.6668764143716714
1101	8	0.6631178604867003
1101	9	0.8411828026230879
1101	10-11	0.7623693316871183
1101	12-13	0.7726362021554607
1101	14-15	0.9164303913364762
1101	16-17	0.8814077449113498
1101	18-19	0.8314108134220888
1101	20-21	0.8562399863335628
1101	22-23	0.8418475562360186
1101	24-25	0.8954582657115324
1101	26-27	0.8219911363865222
1101	28-29	0.8991155402395279
1101	30-31	0.8806951534789391
1101	32-33	0.8444032677782687
1101	34-35	0.8577901262784735
1101	36-37	0.8236925296905682
1101	38-39	0.8915807628745824
1101	40-41	0.9802132248853681
1101	42-43	0.920500567591688
1101	44-45	0.9033854054459383
1101	46-47	0.8868750508693068
1101	48-49	0.7383227976755222
1101	50-51	0.8076813545149477
1101	52-53	0.8309742720757782
1101	54-55	0.90997265487524
1101	56-57	0.8693250095073921
1101	58-59	0.9536990429307721
1101	60-61	0.9074619723205757
1101	62-63	0.9121166149002065
1101	64-65	0.8943447597955583
1101	66-67	0.9381257751911818
1101	68-69	0.9417534530934262
1101	70-71	0.9873504934030422
1101	72-73	1.0722544353831367
1101	74-75	0.9983069136129572
1101	76-77	0.9718639426109519
1101	78-79	0.9126490008216521
1101	80-81	0.8956051436003136
1101	82-83	0.950265065374964
1101	84-85	0.8527113506486756
1101	86-87	0.8725546668459714
1101	88-89	0.8808565541189743
1101	90-91	0.8587171048997355
1101	92-93	0.847705360889659
1101	94-95	0.8800128726874767
1101	96-97	0.8790533046907392
1101	98-99	0.949817339761811
1101	100	0.8991756876504482
1102	1	0.2693131372114479
1102	2	0.2640602049287928
1102	3	0.35853759606296265
1102	4	0.3708472667061855
1102	5	0.2982289086117689
1102	6	0.3974215665186449
1102	7	0.4824776621338742
1102	8	0.48102608394010105
1102	9	0.5525577272410658
1102	10-11	0.5003957091813334
1102	12-13	0.5613255371332286
1102	14-15	0.6090263094076107
1102	16-17	0.5310925504602508
1102	18-19	0.5818899354133649
1102	20-21	0.5317409587566573
1102	22-23	0.5673506091830802
1102	24-25	0.5896382414867389
1102	26-27	0.5878739056645017
1102	28-29	0.5796644652889995
1102	30-31	0.528669009082293
1102	32-33	0.6193146039321746
1102	34-35	0.6352313992988314
1102	36-37	0.6079615696401817
1102	38-39	0.5767666236731301
1102	40-41	0.6155593649119382
1102	42-43	0.5608130859565037
1102	44-45	0.5573249515327987
1102	46-47	0.5103933529724287
1102	48-49	0.4952379564717617
1102	50-51	0.5765463527657388
1102	52-53	0.5537199109622861
1102	54-55	0.5565271691389597
1102	56-57	0.49459699611000474
1102	58-59	0.6467534256580407
1102	60-61	0.5765628980452817
1102	62-63	0.5321839489384814
1102	64-65	0.47988330391784473
1102	66-67	0.586886344943153
1102	68-69	0.6213548030083729
1102	70-71	0.6153367946810491
1102	72-73	0.5547053931932666
1102	74-75	0.5357788857011982
1102	76-77	0.5710620238748305
1102	78-79	0.5269745817216887
1102	80-81	0.47729221386364173
1102	82-83	0.5384140064375664
1102	84-85	0.6266284132722468
1102	86-87	0.6149761462937455
1102	88-89	0.5843903783113511
1102	90-91	0.6704375148201471
1102	92-93	0.6286319059695842
1102	94-95	0.5973677852285633
1102	96-97	0.5831305794922592
1102	98-99	0.6080849574612976
1102	100	0.641079313836098
1103	1	0.20263689180645628
1103	2	0.22820155054348135
1103	3	0.3184799312965261
1103	4	0.321545100644947
1103	5	0.2859205155136806
1103	6	0.34399673433461686
1103	7	0.38989254823591324
1103	8	0.40862740885290094
1103	9	0.5021519591267065
1103	10-11	0.4791232071206153
1103	12-13	0.4460967904825708
1103	14-15	0.5573867598063558
1103	16-17	0.5467723880825446
1103	18-19	0.4989775143129904
1103	20-21	0.4793030936803291
1103	22-23	0.4718379433964017
1103	24-25	0.48625408085415245
1103	26-27	0.4786713327734873
1103	28-29	0.47130677806108423
1103	30-31	0.5052525168753021
1103	32-33	0.5285241704447543
1103	34-35	0.5219464747761506
1103	36-37	0.5052833601177511
1103	38-39	0.5672990596519796
1103	40-41	0.5163943195371843
1103	42-43	0.5681048957448525
1103	44-45	0.44220125679062505
1103	46-47	0.4942869234776026
1103	48-49	0.5475398529762501
1103	50-51	0.47072551249709704
1103	52-53	0.5333015924155404
1103	54-55	0.5267543556740293
1103	56-57	0.5131467846493578
1103	58-59	0.530949140610403
1103	60-61	0.45914051081992113
1103	62-63	0.5318261840142426
1103	64-65	0.5302452012202536
1103	66-67	0.5632857952797323
1103	68-69	0.5900089626611802
1103	70-71	0.537954895240091
1103	72-73	0.5457216649559768
1103	74-75	0.5336907921586231
1103	76-77	0.6192235869116445
1103	78-79	0.4663974265555595
1103	80-81	0.4254253548985716
1103	82-83	0.5148602935671995
1103	84-85	0.5458893612417022
1103	86-87	0.537439758752086
1103	88-89	0.4466421892499035
1103	90-91	0.5247460665482393
1103	92-93	0.4796361309930006
1103	94-95	0.5031498820715115
1103	96-97	0.46328819925005504
1103	98-99	0.44720725747823664
1103	100	0.37658667799107093
1104	1	0.11256768539843165
1104	2	0.08440895703591167
1104	3	0.1679882123905152
1104	4	0.21228530383798727
1104	5	0.2003168894684606
1104	6	0.2394728929500758
1104	7	0.2663959262085811
1104	8	0.2866188103770213
1104	9	0.29428816227856913
1104	10-11	0.27454261442280625
1104	12-13	0.24999458322019308
1104	14-15	0.38958033187950036
1104	16-17	0.36795346045590804
1104	18-19	0.33175033407095356
1104	20-21	0.2853506426343415
1104	22-23	0.32158667987336287
1104	24-25	0.38169153603467976
1104	26-27	0.29944725429609775
1104	28-29	0.31068944454932534
1104	30-31	0.3348172747901188
1104	32-33	0.31437477106853606
1104	34-35	0.30943751088220495
1104	36-37	0.24177424868410924
1104	38-39	0.3570544525707575
1104	40-41	0.38077309166384765
1104	42-43	0.3571953993355521
1104	44-45	0.30215376959741747
1104	46-47	0.26645098761276387
1104	48-49	0.24644284391457205
1104	50-51	0.2582488014655908
1104	52-53	0.27987952939894
1104	54-55	0.26762820237685503
1104	56-57	0.3296111846946488
1104	58-59	0.40358841979324467
1104	60-61	0.34941798837159155
1104	62-63	0.2667613180180268
1104	64-65	0.3150362610012749
1104	66-67	0.3882618629695713
1104	68-69	0.33916664827812326
1104	70-71	0.33482904388461776
1104	72-73	0.3695690883296905
1104	74-75	0.36647445436822323
1104	76-77	0.37733521612724985
1104	78-79	0.349402981596441
1104	80-81	0.38193662452906807
1104	82-83	0.2841363540516113
1104	84-85	0.3299575451653638
1104	86-87	0.37500384416041044
1104	88-89	0.36989787553249087
1104	90-91	0.333246068263815
1104	92-93	0.29198403644407733
1104	94-95	0.3420265217349794
1104	96-97	0.32607031743964043
1104	98-99	0.27623421606761767
1104	100	0.2736332078918764
1105	1	0.2178723474495179
1105	2	0.2424504356918291
1105	3	0.27578166414259186
1105	4	0.2939491808449901
1105	5	0.25622991715709986
1105	6	0.30955361941350645
1105	7	0.3201300296550542
1105	8	0.3662048415904309
1105	9	0.4224200399767213
1105	10-11	0.45659699102289864
1105	12-13	0.44259066380332257
1105	14-15	0.45032865093918417
1105	16-17	0.4660213276654659
1105	18-19	0.46010531419600653
1105	20-21	0.4660234364400253
1105	22-23	0.4581216983457921
1105	24-25	0.410286303785071
1105	26-27	0.39294104252201834
1105	28-29	0.4198680271735995
1105	30-31	0.3923043212286075
1105	32-33	0.4582797933792193
1105	34-35	0.536124617466541
1105	36-37	0.5278375222980571
1105	38-39	0.48777939036948936
1105	40-41	0.5081588222359272
1105	42-43	0.557054100545642
1105	44-45	0.4586182441939215
1105	46-47	0.4105822383519495
1105	48-49	0.5066923524816147
1105	50-51	0.5452369846519218
1105	52-53	0.518163948061563
1105	54-55	0.5077610156771826
1105	56-57	0.4912948465219529
1105	58-59	0.45041154921542415
1105	60-61	0.4794834189706165
1105	62-63	0.43213787370943635
1105	64-65	0.4934307316276332
1105	66-67	0.49301391300669906
1105	68-69	0.41109085395056155
1105	70-71	0.4058412968436613
1105	72-73	0.4072695558230137
1105	74-75	0.39042760655173936
1105	76-77	0.4649855400926839
1105	78-79	0.3892557385671793
1105	80-81	0.4494815965247554
1105	82-83	0.40562827459049444
1105	84-85	0.4707813850824287
1105	86-87	0.4157719084707452
1105	88-89	0.34868375585367417
1105	90-91	0.4611144605185409
1105	92-93	0.4370681109387604
1105	94-95	0.3954881882059986
1105	96-97	0.3857954667161074
1105	98-99	0.44485533280201395
1105	100	0.48760579316554953
1106	1	0.15862707294337497
1106	2	0.13364949724898878
1106	3	0.18562589318240796
1106	4	0.21021079511270102
1106	5	0.2165905081847015
1106	6	0.26712474686825516
1106	7	0.26727300842159707
1106	8	0.2609478562258616
1106	9	0.3498815056126361
1106	10-11	0.32440928212985654
1106	12-13	0.38045750626546493
1106	14-15	0.4323965769395528
1106	16-17	0.4376093844607354
1106	18-19	0.448848204937498
1106	20-21	0.3666487657517479
1106	22-23	0.4191245223806419
1106	24-25	0.4372043966458108
1106	26-27	0.36010354438764125
1106	28-29	0.4428609066485194
1106	30-31	0.4240975491752579
1106	32-33	0.5065992973178624
1106	34-35	0.45250350554456986
1106	36-37	0.3580742802642334
1106	38-39	0.3791933310015807
1106	40-41	0.4326166059575982
1106	42-43	0.447570509253687
1106	44-45	0.38711765402671716
1106	46-47	0.3720478444256088
1106	48-49	0.39591868533421604
1106	50-51	0.4416904977670768
1106	52-53	0.4231663898769824
1106	54-55	0.4381575108804583
1106	56-57	0.3523056999613772
1106	58-59	0.4927128916605952
1106	60-61	0.48870158549760845
1106	62-63	0.3681228788262416
1106	64-65	0.38497406093932796
1106	66-67	0.3950107333352122
1106	68-69	0.42774463872866164
1106	70-71	0.39106376940952714
1106	72-73	0.39173671616364203
1106	74-75	0.4407715256094775
1106	76-77	0.4691049451992342
1106	78-79	0.35507148216464657
1106	80-81	0.41656981062575227
1106	82-83	0.444175550689593
1106	84-85	0.4404395116557538
1106	86-87	0.39495610766655176
1106	88-89	0.4184811156101631
1106	90-91	0.48974471434986455
1106	92-93	0.47256087553130044
1106	94-95	0.48670818776982827
1106	96-97	0.4323389444583974
1106	98-99	0.5145413738756979
1106	100	0.5123499319858205
1107	1	0.15861993564717736
1107	2	0.12998742227622628
1107	3	0.15598015788197728
1107	4	0.19525971877674664
1107	5	0.1650702190695128
1107	6	0.19220271432505598
1107	7	0.2184057006634248
1107	8	0.2620402961182364
1107	9	0.30441811282150866
1107	10-11	0.33454954150654004
1107	12-13	0.3132931435391342
1107	14-15	0.3335157625153542
1107	16-17	0.3758330633547047
1107	18-19	0.37487404034884264
1107	20-21	0.2991782455365808
1107	22-23	0.35201459821374925
1107	24-25	0.335388982000417
1107	26-27	0.3359937870133507
1107	28-29	0.38107350855885613
1107	30-31	0.34765500989603026
1107	32-33	0.3410081180754432
1107	34-35	0.36716363867249413
1107	36-37	0.37377760343937183
1107	38-39	0.41548378900687766
1107	40-41	0.45739007768154494
1107	42-43	0.4548304860681469
1107	44-45	0.3982936468104441
1107	46-47	0.3584436701012237
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2214	96-97	-0.21717893211816985
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2214	100	-0.17343317270565706
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2215	100	-0.45998448212066023
2216	1	-1.3645702469241705
2216	2	-1.2769463900914921
2216	3	-1.435283941867663
2216	4	-1.5914167277647238
2216	5	-1.507102070174696
2216	6	-3.0105920674112205
2216	7	-2.813837415739375
2216	8	-3.701978202864339
2216	9	-3.2259694530868686
2216	10-11	-2.47644048185591
2216	12-13	-2.217102433451778
2216	14-15	-2.461201029734198
2216	16-17	-2.8163100921386572
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2216	32-33	-2.8980577318303204
2216	34-35	-3.842595730892171
2216	36-37	-2.8915168043812116
2216	38-39	-2.823849183334069
2216	40-41	-3.3644896657933288
2216	42-43	-3.197203921252708
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2216	50-51	-3.31937475167787
2216	52-53	-3.5877792674659474
2216	54-55	-3.0094851363387107
2216	56-57	-2.936399322096797
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2216	62-63	-3.63780059025072
2216	64-65	-3.6236311253295312
2216	66-67	-3.3267522825474316
2216	68-69	-3.6667547543350807
2216	70-71	-3.454435701797209
2216	72-73	-3.8049772450044017
2216	74-75	-4.235824964030325
2216	76-77	-4.908899713046061
2216	78-79	-3.982250332403545
2216	80-81	-3.79680496954224
2216	82-83	-3.985011324231074
2216	84-85	-4.054770693952193
2216	86-87	-3.6807890785831887
2216	88-89	-3.607887983219701
2216	90-91	-3.812625354285
2216	92-93	-3.8363576485000763
2216	94-95	-3.6846731854440478
2216	96-97	-3.980053719418752
2216	98-99	-3.784909089340612
2216	100	-3.4461531189469454
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125221.0
3	21765.0
4	14358.0
5	11511.0
6	10717.0
7	11283.0
8	12798.0
9	14606.0
10	16433.0
11	18216.0
12	20253.0
13	22089.0
14	24738.0
15	25288.0
16	25460.0
17	25825.0
18	24950.0
19	24275.0
20	24409.0
21	25475.0
22	28522.0
23	34141.0
24	41757.0
25	52437.0
26	63930.0
27	74182.0
28	88743.0
29	109939.0
30	132035.0
31	157280.0
32	198386.0
33	259603.0
34	353308.0
35	504507.0
36	805590.0
37	1333413.0
38	1618014.0
39	529642.0
40	1171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89531139190208	20.675674046688126	14.210725287786852	23.21828927362295
2	15.271506424428924	20.50576144575653	40.80354896458996	23.419183165224585
3	18.063312114438798	25.487499271857956	30.695301933088214	25.753886680615036
4	12.50216087735543	16.96658433784471	39.29356815399915	31.237686630800702
5	12.824808051131356	37.89693596552039	35.85678374293466	13.421472240413598
6	30.490287102101977	38.15811513167787	17.896010200507614	13.455587565712545
7	26.452071862752373	32.72969768002332	23.10181118297296	17.71641927425134
8	24.28017495100059	36.85660851430527	20.92973661472022	17.933479919973923
9	24.534489836771826	17.153558139487586	22.12636506502717	36.18558695871342
10-11	23.424090620926975	27.260329752511293	29.437579281398918	19.87800034516281
12-13	23.762882820114868	24.916967918619154	29.34401877380329	21.97613048746269
14-15	21.30710290271197	27.19625319684735	26.470731368944328	25.025912531496346
16-17	20.8458524602059	30.463468989862168	27.167375088184986	21.523303461746945
18-19	20.772117491996756	29.469058456476837	29.09694410229509	20.661879949231317
20-21	21.94772811297224	28.712065911126317	28.56204008968864	20.778165886212804
22-23	21.551984120297117	28.631585721515982	28.602698875439515	21.213731282747382
24-25	21.435721597750597	28.67806476889078	28.57820935151649	21.308004281842134
26-27	21.112952673461653	28.668244878902726	28.637579343079146	21.581223104556475
28-29	21.198933890381298	28.667860513814286	28.78711690998172	21.346088685822696
30-31	21.490221941535385	29.066769127853618	28.332847296393798	21.110161634217196
32-33	21.09760823135356	28.82271699020435	28.778345485440894	21.301329293001192
34-35	21.314209744326057	28.4810741664202	28.775383051875647	21.4293330373781
36-37	21.634998933772454	28.786959891831653	28.272141943648567	21.30589923074733
38-39	21.390218814410638	28.938482896630806	28.430301161249048	21.240997127709505
40-41	21.265351024050453	28.642834450442745	28.67677553719959	21.41503898830721
42-43	21.17035435253266	28.663063524768766	28.626660228541812	21.539921894156763
44-45	21.30537663414305	28.736419107608434	28.522628568299197	21.435575689949317
46-47	21.30525261648856	28.891772977591508	28.34648265699767	21.456491748922268
48-49	21.24650291355174	28.713662676156233	28.472390130711627	21.5674442795804
50-51	21.31310521708265	28.604272616615063	28.41451509239076	21.66810707391152
52-53	21.2251423567711	28.628762143423515	28.486062186443412	21.660033313361975
54-55	21.341891940574637	28.952229380784374	28.290302065756507	21.415576612884482
56-57	21.231857547854936	28.724378971478675	28.32573219306424	21.718031287602148
58-59	21.14528575637017	28.317012670907925	28.62968898257961	21.90801259014229
60-61	21.349535861497536	28.686902903945178	28.29880862595713	21.664752608600157
62-63	21.221739073909056	28.71753066237351	28.308314072243995	21.752416191473447
64-65	21.248270344281018	28.469116330767985	28.386653550867347	21.895959774083646
66-67	21.126499199550512	28.483429656969516	28.29889267643553	22.091178467044447
68-69	21.450309110237953	28.59434026740529	28.206742270283037	21.748608352073724
70-71	21.380169142896985	28.41777888822391	28.31232604893581	21.889725919943288
72-73	21.165462755048793	28.477038790679455	28.476116401453133	21.881382052818623
74-75	21.24635228394107	28.476701281551346	28.37541245274465	21.901533981762928
76-77	21.290169777071043	28.460045697171658	28.256544464972027	21.993240060785272
78-79	21.332751809756086	28.50216037703645	28.3404353292278	21.824652483979655
80-81	21.286168234060003	28.35693847092339	28.389636530777974	21.967256764238634
82-83	21.146938150307196	28.360887233978733	28.55193803809526	21.940236577618812
84-85	21.206883052572604	28.254827385497805	28.395947365370596	22.142342196559
86-87	21.36163553668335	28.339353641578345	28.336455700946473	21.96255512079183
88-89	21.247418261938748	28.196207516940497	28.425937445913057	22.130436775207706
90-91	21.181843213015895	28.08466973308853	28.468632195192527	22.26485485870305
92-93	21.292383906613253	28.248018678628107	28.30310107961942	22.156496335139224
94-95	21.19647021465806	28.008654072146516	28.48658470651022	22.308291006685206
96-97	21.290560782542652	27.98456203430885	28.41193418207535	22.312943001073148
98-99	21.27299684734987	28.013750550007476	28.36977783328275	22.3434747693599
100	21.313933377575957	27.966852882620053	28.300037611072465	22.419176128731518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2011.0
1	1671.5
2	1623.0
3	1916.0
4	1794.0
5	1496.0
6	1204.5
7	1041.5
8	990.0
9	963.5
10	935.0
11	913.5
12	912.5
13	975.5
14	1161.5
15	1382.0
16	1639.0
17	2110.5
18	2885.5
19	4150.5
20	6086.5
21	8829.5
22	12781.5
23	19072.5
24	27844.5
25	39117.0
26	53888.0
27	70896.0
28	90941.5
29	113881.5
30	135900.5
31	155161.0
32	171287.0
33	186457.0
34	201954.0
35	214707.5
36	224888.5
37	236350.5
38	246311.0
39	251524.0
40	254315.5
41	255395.5
42	253050.5
43	249153.5
44	243808.0
45	238052.5
46	231616.5
47	222524.5
48	213931.0
49	207076.0
50	201515.5
51	192461.5
52	178515.5
53	166495.5
54	157900.5
55	150582.5
56	139798.5
57	126362.5
58	113808.0
59	101999.0
60	89827.0
61	76790.0
62	65378.5
63	54844.5
64	44832.5
65	36359.5
66	29226.0
67	22446.5
68	17324.0
69	13746.5
70	10453.5
71	7768.0
72	5822.5
73	4201.0
74	2959.0
75	2123.5
76	1472.0
77	1013.0
78	712.5
79	513.0
80	363.5
81	241.5
82	175.0
83	125.0
84	100.0
85	83.5
86	56.0
87	35.0
88	29.0
89	20.5
90	18.0
91	22.5
92	17.0
93	12.5
94	13.5
95	12.5
96	15.5
97	15.5
98	12.5
99	14.0
100	34.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012764529999549828
2	0.032441365209322316
3	0.03355953222862304
4	0.03618795080645981
5	0.03871471783708742
6	0.039077759077120126
7	0.03684142503851868
8	0.03650742709768859
9	0.03676881679051214
10-11	0.03720446627855138
12-13	0.038271807524247525
14-15	0.03681238173931606
16-17	0.03345061985661323
18-19	0.036289602353668966
20-21	0.035650649771211414
22-23	0.03650742709768859
24-25	0.03364666212623089
26-27	0.03369748789983547
28-29	0.03430739718309041
30-31	0.03430739718309041
32-33	0.034089572439070785
34-35	0.034067789964668825
36-37	0.03174432602845953
38-39	0.03219449716610008
40-41	0.033152926039786416
42-43	0.033080317791779876
44-45	0.03188228169967195
46-47	0.03106180849719805
48-49	0.031170720869207857
50-51	0.03225984458930597
52-53	0.032608364179737365
54-55	0.03203475902048569
56-57	0.030575333235554225
58-59	0.031279633241217665
60-61	0.030756853855570577
62-63	0.03108359097160001
64-65	0.02834626002175343
66-67	0.029232080647433226
68-69	0.030139683747514984
70-71	0.031221546642812438
72-73	0.028672997137782864
74-75	0.02899247342901164
76-77	0.029573339413063966
78-79	0.029841989930688165
80-81	0.030052553849907133
82-83	0.028687518787384174
84-85	0.029871033229890785
86-87	0.030074336324309094
88-89	0.030488203337946378
90-91	0.030981939424390852
92-93	0.029602382712266586
94-95	0.030669723957962726
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6886270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.77015672472936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2690717322326	47.111525141496344
2	14.29981331502837	17.666034192073486
3	4.402748793580796	8.158754489972967
4	1.7985876162000969	4.443961557433496
5	0.9321281567675209	2.8788851165531435
6	0.5395701715645909	1.9997600436920333
7	0.34595396459155653	1.4958741428653346
8	0.25003967649052783	1.2355992003376428
9	0.18148433185914173	1.008928405981978
>10	0.9118753734465311	9.836743581258249
>50	0.04950268504453718	2.08573122552327
>100	0.018894448931191816	1.9192972635869374
>500	3.0618503731734913E-4	0.1170265728013236
>1k	2.3549225241734336E-5	0.041879066423783744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.452164960130811E-5	0.0
3	0.0	0.0	0.0	2.904329920261622E-5	0.0
4	0.0	0.0	0.0	1.1617319681046488E-4	0.0
5	0.0	0.0	0.0	1.1617319681046488E-4	0.0
6	0.0	0.0	0.0	1.1617319681046488E-4	0.0
7	0.0	0.0	0.0	1.1617319681046488E-4	0.0
8	0.0	0.0	0.0	1.597381456143892E-4	0.0
9	0.0	0.0	0.0	1.7425979521569731E-4	0.0
10-11	1.452164960130811E-5	0.0	0.0	2.0330309441831356E-4	0.0
12-13	1.452164960130811E-5	0.0	0.0	2.3234639362092977E-4	0.0
14-15	1.452164960130811E-5	0.0	0.0	2.976938168268163E-4	0.0
16-17	2.1782474401962168E-5	7.260824800654055E-6	0.0	3.4125876563074055E-4	0.0
18-19	2.904329920261622E-5	3.6304124003270275E-5	0.0	3.9208453923531895E-4	0.0
20-21	2.904329920261622E-5	5.808659840523244E-5	0.0	4.5017113764055143E-4	0.0
22-23	2.904329920261622E-5	6.53474232058865E-5	0.0	7.551257792680218E-4	0.0
24-25	2.904329920261622E-5	7.260824800654055E-5	0.0	0.0012851659897157677	0.0
26-27	2.904329920261622E-5	8.712989760784866E-5	0.0	0.0021855082649968704	0.0
28-29	2.904329920261622E-5	8.712989760784866E-5	0.0	0.006026484584542866	0.0
30-31	2.904329920261622E-5	8.712989760784866E-5	0.0	0.01802862798002402	0.0
32-33	2.904329920261622E-5	1.0165154720915678E-4	0.0	0.047079188007440896	0.0
34-35	2.904329920261622E-5	1.1617319681046488E-4	0.0	0.0946303296269243	0.0
36-37	2.904329920261622E-5	1.1617319681046488E-4	0.0	0.1674999673262884	0.0
38-39	2.904329920261622E-5	1.1617319681046488E-4	0.0	0.26149134437075516	0.0
40-41	2.904329920261622E-5	1.30694846411773E-4	0.0	0.35753027400900633	0.0
42-43	2.904329920261622E-5	1.30694846411773E-4	0.0	0.4512820438350515	0.0
44-45	2.904329920261622E-5	1.3795567121242705E-4	0.0	0.567840064359951	0.0
46-47	2.904329920261622E-5	1.452164960130811E-4	0.0	0.7067178603220612	0.0
48-49	2.904329920261622E-5	1.452164960130811E-4	0.0	0.8501191501349787	0.0
50-51	2.904329920261622E-5	1.597381456143892E-4	0.0	0.9924676203518015	0.0
52-53	2.904329920261622E-5	1.7425979521569731E-4	0.0	1.1297044699089638	0.0
54-55	2.904329920261622E-5	1.7425979521569731E-4	0.0	1.2808240164849767	0.0
56-57	2.904329920261622E-5	1.8152062001635137E-4	0.0	1.4606456034979751	0.0
58-59	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	1.6509590823479185	0.0
60-61	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	1.8461736179383035	0.0
62-63	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	2.03452667409207	0.0
64-65	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	2.2331683189883638	0.0
66-67	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	2.462443383718617	0.0
68-69	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	2.7114824135562503	0.0
70-71	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	2.96947404037309	0.0
72-73	2.904329920261622E-5	1.8878144481700542E-4	1.452164960130811E-5	3.233419543526466	0.0
74-75	2.904329920261622E-5	2.0330309441831356E-4	1.452164960130811E-5	3.4997611188640585	0.0
76-77	2.904329920261622E-5	2.0330309441831356E-4	1.452164960130811E-5	3.783289066504799	0.0
78-79	2.904329920261622E-5	2.0330309441831356E-4	1.452164960130811E-5	4.097639215424316	0.0
80-81	2.904329920261622E-5	2.105639192189676E-4	1.452164960130811E-5	4.431550026356794	0.0
82-83	2.904329920261622E-5	2.3234639362092977E-4	1.452164960130811E-5	4.77153814764742	0.0
84-85	2.904329920261622E-5	2.3234639362092977E-4	1.452164960130811E-5	5.103110972994088	0.0
86-87	2.904329920261622E-5	2.3234639362092977E-4	1.452164960130811E-5	5.454789022213767	7.260824800654055E-6
88	2.904329920261622E-5	2.3234639362092977E-4	1.452164960130811E-5	5.734947947147004	1.452164960130811E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6540	0.0	44.925186	1
GTATCAA	11015	0.0	34.014294	1
TCAACGC	11815	0.0	30.714901	4
ATCAACG	11990	0.0	30.266602	3
CAACGCA	12360	0.0	29.436626	5
AACGCAG	13385	0.0	27.14395	6
TATCAAC	13570	0.0	26.846481	2
GTACATG	18105	0.0	25.809254	1
TACATGG	18120	0.0	24.541353	2
ACGCAGA	15440	0.0	23.044142	7
CGCAGAG	15460	0.0	22.649506	8
ACATGGG	18875	0.0	22.63823	3
GTGGTAT	4460	0.0	19.815756	1
CATGGGG	12775	0.0	19.686043	4
TGGTATC	4155	0.0	18.893436	2
GCAGAGT	17750	0.0	18.721169	9
GAGTACA	13815	0.0	17.082067	1
CAGAGTA	16935	0.0	16.8606	10-11
AGAGTAC	15400	0.0	16.664177	10-11
AGTACAT	14550	0.0	15.055266	2
>>END_MODULE