FastQCFastQC Report
Wed 25 May 2016
SRR2049386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6886270
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163150.23692071324534184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159500.23162031114086437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88860.12903937835722387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114400.059.179071
GTATCAA209200.039.598241
ATCAACG259950.031.5840323
TCAACGC262000.031.3369054
TATCAAC270750.030.3936022
CAACGCA271850.030.2014685
AACGCAG283800.029.0349146
GTGGTAT51400.028.6294421
TGGTATC51550.027.0764852
ACGCAGA325450.025.0880817
CGCAGAG329300.024.6519378
GTACATG182000.022.9905971
GCAGAGT360550.022.1500249
TACATGG181250.021.7285392
GAGTACT259150.020.6909512-13
ACATGGG186650.020.5459693
CAGAGTA348400.020.53303310-11
AGAGTAC331850.020.1822810-11
AGTACTT269400.019.04857612-13
GTACTTT284700.018.66993314-15
Produced by FastQC (version 0.11.5)