##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049386_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6886270
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818843728172144	31.0	31.0	34.0	30.0	34.0
2	31.885893088711306	31.0	31.0	34.0	30.0	34.0
3	31.893838464074165	33.0	31.0	34.0	30.0	34.0
4	35.65755074953494	37.0	35.0	37.0	33.0	37.0
5	35.7725950333054	37.0	35.0	37.0	35.0	37.0
6	35.84772583706419	37.0	35.0	37.0	35.0	37.0
7	35.785420554233276	37.0	35.0	37.0	33.0	37.0
8	35.78463928948473	37.0	35.0	37.0	33.0	37.0
9	37.51220225172699	39.0	37.0	39.0	35.0	39.0
10-11	37.48166794215156	39.0	37.0	39.0	34.5	39.0
12-13	37.44304776025338	39.0	37.0	39.0	34.5	39.0
14-15	38.73454373703035	40.0	38.0	41.0	34.5	41.0
16-17	38.76407779538124	40.0	38.0	41.0	35.0	41.0
18-19	38.83523337016992	40.0	38.0	41.0	35.0	41.0
20-21	38.86349446071676	40.0	38.0	41.0	35.0	41.0
22-23	38.83010316179877	40.0	38.0	41.0	35.0	41.0
24-25	38.8168895497853	40.0	38.0	41.0	35.0	41.0
26-27	38.709175431692344	40.0	38.0	41.0	34.5	41.0
28-29	38.621850290505606	40.0	38.0	41.0	34.0	41.0
30-31	38.513923357637736	40.0	38.0	41.0	34.0	41.0
32-33	38.373580109406106	40.0	38.0	41.0	34.0	41.0
34-35	38.27165482910196	40.0	38.0	41.0	34.0	41.0
36-37	38.157442853678404	40.0	38.0	41.0	34.0	41.0
38-39	38.06001667085374	40.0	38.0	41.0	33.5	41.0
40-41	37.944030730714886	40.0	38.0	41.0	33.0	41.0
42-43	37.8049463642872	40.0	37.0	41.0	33.0	41.0
44-45	37.62917239666757	40.0	37.0	41.0	33.0	41.0
46-47	37.396539055250514	40.0	37.0	41.0	32.5	41.0
48-49	37.18827819705007	40.0	37.0	41.0	32.0	41.0
50-51	37.04411960321045	40.0	37.0	41.0	31.0	41.0
52-53	36.91094983205713	40.0	36.5	41.0	31.0	41.0
54-55	36.77741259056064	40.0	36.0	41.0	31.0	41.0
56-57	36.6416882434177	39.0	36.0	41.0	31.0	41.0
58-59	36.46156925302087	39.0	35.0	41.0	31.0	41.0
60-61	36.288899796261255	39.0	35.0	41.0	30.5	41.0
62-63	36.09397997754953	39.0	35.0	41.0	30.0	41.0
64-65	35.87019438680156	38.5	35.0	40.5	30.0	41.0
66-67	35.611133530924576	38.0	35.0	40.0	29.5	41.0
68-69	35.325244290450414	37.0	35.0	40.0	29.0	41.0
70-71	34.99659009884887	37.0	34.0	39.5	29.0	41.0
72-73	34.706396496216385	36.0	34.0	39.0	29.0	41.0
74-75	34.39917008772528	36.0	34.0	39.0	29.0	41.0
76-77	34.070583930052116	35.5	34.0	38.5	29.0	40.0
78-79	33.73257068049902	35.0	34.0	37.0	28.5	39.5
80-81	33.4111874062446	35.0	34.0	37.0	28.0	39.0
82-83	33.1076688105462	35.0	34.0	36.5	28.0	39.0
84-85	32.83258251564345	35.0	34.0	36.0	27.0	37.5
86-87	32.59465443556526	35.0	33.0	36.0	27.0	37.0
88-89	32.36098265098522	35.0	33.0	35.5	27.0	37.0
90-91	32.16043467653752	35.0	33.0	35.0	26.5	36.0
92-93	31.978365864248715	35.0	33.0	35.0	26.0	36.0
94-95	31.816403655389635	35.0	33.0	35.0	26.0	36.0
96-97	31.62333491135259	35.0	33.0	35.0	25.5	36.0
98-99	31.472777062183155	35.0	33.0	35.0	25.0	35.5
100	31.358942359216236	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17826718917832451
1101	2	0.1828075550153052
1101	3	0.40641140913750107
1101	4	0.23466010944678573
1101	5	0.1884370813820624
1101	6	0.18118022810543266
1101	7	0.0768518219028067
1101	8	0.14280566879965306
1101	9	0.2686432985550411
1101	10-11	0.3025184364955251
1101	12-13	0.21671586771909546
1101	14-15	0.33262179688681925
1101	16-17	0.26415389855562665
1101	18-19	0.37850455919920023
1101	20-21	0.3043880895323383
1101	22-23	0.31225716011636706
1101	24-25	0.33654912701073414
1101	26-27	0.3875994498461708
1101	28-29	0.3904226976994849
1101	30-31	0.39489592578365773
1101	32-33	0.4057490425139818
1101	34-35	0.39516112399128644
1101	36-37	0.373833817146334
1101	38-39	0.3731208692257866
1101	40-41	0.41921808416147144
1101	42-43	0.4298431856842839
1101	44-45	0.38132910720010926
1101	46-47	0.4174688406490574
1101	48-49	0.3923875044782861
1101	50-51	0.3264953059566267
1101	52-53	0.3877120836299852
1101	54-55	0.3270484847464701
1101	56-57	0.2885569998658539
1101	58-59	0.34364237380984974
1101	60-61	0.31300292339073366
1101	62-63	0.32678362342397804
1101	64-65	0.2463664281600657
1101	66-67	0.22322148905946193
1101	68-69	0.24611131783566265
1101	70-71	0.26438383324909154
1101	72-73	0.21394356904217204
1101	74-75	0.26159898804178994
1101	76-77	0.21282531588575893
1101	78-79	0.29137600993384893
1101	80-81	0.31116501592189394
1101	82-83	0.273081423871659
1101	84-85	0.30335109691464623
1101	86-87	0.24101396819693122
1101	88-89	0.3115702022099285
1101	90-91	0.3198320770461791
1101	92-93	0.3509718281030736
1101	94-95	0.39339997451306985
1101	96-97	0.4221290616686062
1101	98-99	0.48825630233315565
1101	100	0.5409998625633605
1102	1	0.17477512336986933
1102	2	0.1506291852862809
1102	3	0.31849808987941586
1102	4	0.17766272558242235
1102	5	0.14873194783214672
1102	6	0.14520378791941368
1102	7	0.1547759835502518
1102	8	0.16895554777428856
1102	9	0.16069376437548044
1102	10-11	0.21412265260339325
1102	12-13	0.1750372043502395
1102	14-15	0.2620841461079877
1102	16-17	0.21256427964056712
1102	18-19	0.23988811158245227
1102	20-21	0.22670261624370625
1102	22-23	0.22394892585607096
1102	24-25	0.23533657594915525
1102	26-27	0.3227850542768351
1102	28-29	0.32473232265693497
1102	30-31	0.24208552245386983
1102	32-33	0.27544283868731156
1102	34-35	0.3380366309587828
1102	36-37	0.30415906121842085
1102	38-39	0.2657938652867955
1102	40-41	0.22680873288659598
1102	42-43	0.2667621328089922
1102	44-45	0.32185384887002755
1102	46-47	0.3500236373118568
1102	48-49	0.3052521730005324
1102	50-51	0.35847636115894943
1102	52-53	0.3992653614116861
1102	54-55	0.3073075464636901
1102	56-57	0.373171002521822
1102	58-59	0.3482477270078874
1102	60-61	0.34112819578160014
1102	62-63	0.38208883136002214
1102	64-65	0.2691184170818133
1102	66-67	0.2926230554277822
1102	68-69	0.29899669891074154
1102	70-71	0.28415950937993983
1102	72-73	0.2848040757276493
1102	74-75	0.2642341935048691
1102	76-77	0.2867132906149834
1102	78-79	0.3421963378559738
1102	80-81	0.3096718504924283
1102	82-83	0.35740571109992914
1102	84-85	0.3231148815198708
1102	86-87	0.30241385910254337
1102	88-89	0.3273873117291046
1102	90-91	0.3520868838453737
1102	92-93	0.40284399400542625
1102	94-95	0.3602969942955987
1102	96-97	0.4215251772613371
1102	98-99	0.44419662225660517
1102	100	0.432590956579773
1103	1	0.1596849351686096
1103	2	0.1708627655815782
1103	3	0.3504694332650935
1103	4	0.19832798705008514
1103	5	0.1863675819574766
1103	6	0.1073526988330542
1103	7	0.16774226267792613
1103	8	0.16716381906244493
1103	9	0.2581891052143206
1103	10-11	0.2116070055058259
1103	12-13	0.22777628075610323
1103	14-15	0.24920644174498818
1103	16-17	0.27050404442064035
1103	18-19	0.2936080850704883
1103	20-21	0.26829972993942874
1103	22-23	0.24049905755420298
1103	24-25	0.24373037387795904
1103	26-27	0.2971240142143827
1103	28-29	0.3415026272731936
1103	30-31	0.34132167520218815
1103	32-33	0.32989406696054857
1103	34-35	0.33612260670514615
1103	36-37	0.38835298610835167
1103	38-39	0.38085930206631247
1103	40-41	0.3902510305574083
1103	42-43	0.431636128124552
1103	44-45	0.4784427830278162
1103	46-47	0.4350676085767944
1103	48-49	0.38760801322680294
1103	50-51	0.3926417497876855
1103	52-53	0.4384896426183005
1103	54-55	0.4166476637527694
1103	56-57	0.41138344617889544
1103	58-59	0.44825348282031996
1103	60-61	0.44375950185970936
1103	62-63	0.4302265760927426
1103	64-65	0.4381145833965192
1103	66-67	0.4000766992379212
1103	68-69	0.3714371024479348
1103	70-71	0.4166406206506963
1103	72-73	0.4244152865458801
1103	74-75	0.38257674423147847
1103	76-77	0.36294924540904816
1103	78-79	0.405539096629731
1103	80-81	0.43859282751992623
1103	82-83	0.4000335865424347
1103	84-85	0.35885918614584256
1103	86-87	0.35985649117225194
1103	88-89	0.4260709017221558
1103	90-91	0.455026704326869
1103	92-93	0.4612754784613564
1103	94-95	0.39638894713659667
1103	96-97	0.4895099773461169
1103	98-99	0.5351745219363622
1103	100	0.5814879020191448
1104	1	0.1635105363928453
1104	2	0.24210302538867623
1104	3	0.3146642268296169
1104	4	0.18882039218721047
1104	5	0.172346507799908
1104	6	0.1492421675872322
1104	7	0.20234574118344995
1104	8	0.18121440115798748
1104	9	0.20409131041800777
1104	10-11	0.18754252723018539
1104	12-13	0.20904073021004166
1104	14-15	0.23671779029206874
1104	16-17	0.2433971604932097
1104	18-19	0.22910929112167366
1104	20-21	0.3059506235838967
1104	22-23	0.27216622173842353
1104	24-25	0.2998174840558008
1104	26-27	0.2863347454965748
1104	28-29	0.32629512813957007
1104	30-31	0.32162972435006054
1104	32-33	0.2958214568461912
1104	34-35	0.2953723502262662
1104	36-37	0.23971683613940087
1104	38-39	0.23949349627658023
1104	40-41	0.24489575155299548
1104	42-43	0.2768862078989258
1104	44-45	0.20304416852844298
1104	46-47	0.23997422967770632
1104	48-49	0.2556630626868994
1104	50-51	0.24742920685535097
1104	52-53	0.2033835684435843
1104	54-55	0.181634299401793
1104	56-57	0.1409739377605561
1104	58-59	0.2168792020763135
1104	60-61	0.20598989023221037
1104	62-63	0.22632528357643622
1104	64-65	0.17092720455919874
1104	66-67	0.2126904506232492
1104	68-69	0.16077691380301218
1104	70-71	0.19295679477497885
1104	72-73	0.17086002606970396
1104	74-75	0.17025587628635463
1104	76-77	0.14534110396309785
1104	78-79	0.18426407921661792
1104	80-81	0.1952884874512364
1104	82-83	0.19422086587623966
1104	84-85	0.1864329817244581
1104	86-87	0.18241743707321945
1104	88-89	0.20472293794144036
1104	90-91	0.20375727969734214
1104	92-93	0.2682735947569519
1104	94-95	0.2503926596469306
1104	96-97	0.28777875348723825
1104	98-99	0.2847322802938024
1104	100	0.28330442440572057
1105	1	0.21029592956778842
1105	2	0.1634876990116858
1105	3	0.28582946041580115
1105	4	0.21166200239019872
1105	5	0.1728352807937199
1105	6	0.15269981554141765
1105	7	0.13455167390362988
1105	8	0.15393311245393448
1105	9	0.1838085369731104
1105	10-11	0.21075542376744494
1105	12-13	0.15876152682604783
1105	14-15	0.26615177296320525
1105	16-17	0.26362285692987797
1105	18-19	0.21560113471959141
1105	20-21	0.2338611047463317
1105	22-23	0.24874921620175172
1105	24-25	0.25578491120698743
1105	26-27	0.2774286526072842
1105	28-29	0.3161997213394798
1105	30-31	0.2804293880960458
1105	32-33	0.303334887441693
1105	34-35	0.30432791395954695
1105	36-37	0.2762161715892759
1105	38-39	0.2672995091912327
1105	40-41	0.28153243762197633
1105	42-43	0.2640363628140676
1105	44-45	0.25265427566983334
1105	46-47	0.2761722247279863
1105	48-49	0.22159994647218895
1105	50-51	0.25665285930024595
1105	52-53	0.32587054673435745
1105	54-55	0.3054638052517191
1105	56-57	0.22908102727719637
1105	58-59	0.3001358890158201
1105	60-61	0.2782155506165438
1105	62-63	0.283902016803637
1105	64-65	0.2655411081877759
1105	66-67	0.29159791086345166
1105	68-69	0.23905748541724137
1105	70-71	0.21076073248181615
1105	72-73	0.28253771288127183
1105	74-75	0.26971143760149374
1105	76-77	0.25225076339599184
1105	78-79	0.2858179438295352
1105	80-81	0.2591412734397238
1105	82-83	0.2771761112937412
1105	84-85	0.29161247181247774
1105	86-87	0.2616794017234625
1105	88-89	0.339912175350932
1105	90-91	0.36206556764649633
1105	92-93	0.38960897779766057
1105	94-95	0.3867851174975705
1105	96-97	0.40991598012147534
1105	98-99	0.4309091363627502
1105	100	0.42000317325765124
1106	1	0.14670117827138895
1106	2	0.13870650795923822
1106	3	0.2739489597131133
1106	4	0.19972827640323487
1106	5	0.16820948949709447
1106	6	0.1740931741956473
1106	7	0.16275348678937718
1106	8	0.1484031477954204
1106	9	0.2313286215251722
1106	10-11	0.2144019908968957
1106	12-13	0.1813429994925002
1106	14-15	0.2512996678942514
1106	16-17	0.24306416246842844
1106	18-19	0.2584835646107706
1106	20-21	0.21510090142182037
1106	22-23	0.21276820788348516
1106	24-25	0.20571630063551538
1106	26-27	0.23172824103080814
1106	28-29	0.26419805165301824
1106	30-31	0.24701272929992513
1106	32-33	0.18989154131923414
1106	34-35	0.20551068071912226
1106	36-37	0.2663421950463558
1106	38-39	0.23367415237592581
1106	40-41	0.2352284352937346
1106	42-43	0.2766932808503171
1106	44-45	0.25812071186354757
1106	46-47	0.2539613084856214
1106	48-49	0.2562024131073244
1106	50-51	0.2303667005290322
1106	52-53	0.21730251047248572
1106	54-55	0.2710384450466492
1106	56-57	0.22160162583733012
1106	58-59	0.28488825605752766
1106	60-61	0.215783353601104
1106	62-63	0.24205211309424612
1106	64-65	0.22005365316476855
1106	66-67	0.2111228625408117
1106	68-69	0.24871071301365788
1106	70-71	0.26380945169971426
1106	72-73	0.30853465365937893
1106	74-75	0.2997890474491314
1106	76-77	0.2920720232915883
1106	78-79	0.3066574713622501
1106	80-81	0.2988982801950044
1106	82-83	0.30745291738587355
1106	84-85	0.26148025979711775
1106	86-87	0.2713937706183174
1106	88-89	0.2991036260760893
1106	90-91	0.3307840074955095
1106	92-93	0.33914019288801356
1106	94-95	0.32201203957103175
1106	96-97	0.38642021847567065
1106	98-99	0.39286068851451716
1106	100	0.42374591761469205
1107	1	0.16539429898177715
1107	2	0.10438016549636231
1107	3	0.25541286785705
1107	4	0.18661130175501484
1107	5	0.13564900249602374
1107	6	0.10605697322900198
1107	7	0.12592020238876955
1107	8	0.12154860392417532
1107	9	0.14774074408268945
1107	10-11	0.15249365065611187
1107	12-13	0.17228852631384228
1107	14-15	0.22371682798210912
1107	16-17	0.16290141177048412
1107	18-19	0.15076526832206127
1107	20-21	0.13553007521341698
1107	22-23	0.11326997147936169
1107	24-25	0.18254092872985694
1107	26-27	0.2628951667802184
1107	28-29	0.23370620906561612
1107	30-31	0.22521081164851608
1107	32-33	0.2620682287219722
1107	34-35	0.26091046552630814
1107	36-37	0.34371825681505186
1107	38-39	0.3079670439324218
1107	40-41	0.3145021590409556
1107	42-43	0.39509611788552945
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2216	86-87	-1.109401018500094
2216	88-89	-1.4650453744232905
2216	90-91	-1.2431944166592785
2216	92-93	-1.3625095202757258
2216	94-95	-1.3215766356997243
2216	96-97	-1.4162921212325017
2216	98-99	-1.6181056206959639
2216	100	-1.543950323738514
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	320.0
3	42.0
4	38.0
5	107.0
6	326.0
7	839.0
8	1868.0
9	3418.0
10	5276.0
11	7762.0
12	11427.0
13	15989.0
14	19230.0
15	24705.0
16	30274.0
17	41521.0
18	39468.0
19	22014.0
20	16594.0
21	16347.0
22	17433.0
23	19356.0
24	22964.0
25	28485.0
26	36321.0
27	47603.0
28	62827.0
29	82169.0
30	107494.0
31	138616.0
32	179298.0
33	235013.0
34	319887.0
35	461269.0
36	745191.0
37	1347640.0
38	1941496.0
39	832094.0
40	3549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.20202847352994	21.038301972007602	14.681025602585319	23.07864395187714
2	15.545542071397142	20.815564884908667	40.09870365234009	23.5401893913541
3	17.670800012198185	25.80953113949932	31.146193803031245	25.373475045271242
4	12.53044646685933	17.552993515935412	38.53873223329853	31.377827783906724
5	12.36856817987096	37.899007735682744	35.95906056544399	13.773363519002304
6	30.146291098083577	38.63650423233478	17.384055519170754	13.83314915041089
7	25.878610626652744	33.33714768662861	22.881879449977998	17.902362236740647
8	24.413779723430835	36.75078424578659	20.53184657971251	18.303589451070064
9	24.501696854755913	16.672596340253868	22.10770126643306	36.71800553855716
10-11	23.46056718659013	27.20515605690744	29.11134039182315	20.22293636467928
12-13	24.14010621661844	24.83539852733256	29.418888520202252	21.605606735846745
14-15	21.442445843623144	26.81329870980802	27.15983649765286	24.584418948915975
16-17	20.32945561667719	30.173879353246292	27.917686631178658	21.578978398897863
18-19	20.183603144549103	28.424129159702016	30.889384093599066	20.502883602149815
20-21	21.299283523018904	27.647347847298054	30.702109975589813	20.351258654093233
22-23	20.918153078517108	27.569460680455453	30.801544232218603	20.710842008808832
24-25	20.829293284120368	27.617106002807617	30.74820283460066	20.80539787847135
26-27	20.585750167707058	27.6327212863696	30.79659237553137	20.98493617039197
28-29	20.614115530307156	27.60483398426864	30.95594914209413	20.825101343330076
30-31	20.959490475939113	27.964233755301038	30.476355884706518	20.59991988405333
32-33	20.603194553205824	27.74853957214029	30.849761898797638	20.798503975856246
34-35	20.805609660856696	27.46209373647216	30.828643924868977	20.90365267780217
36-37	21.13862619401654	27.79256849523693	30.236695125012282	20.83211018573425
38-39	20.919863896836695	27.9553434548601	30.32871896694963	20.796073681353576
40-41	20.82690628204752	27.716533420618283	30.462313494498755	20.994246802835445
42-43	20.78550482019635	27.751591072856368	30.29751094720278	21.1653931597445
44-45	20.965345504835398	27.928344739760007	30.0700403027802	21.036269452624392
46-47	21.122192272091883	28.116247973138403	29.601553063185804	21.16000669158391
48-49	21.085338827212478	28.0520457627702	29.48815523897111	21.374460171046216
50-51	21.151983463825523	28.03545362081695	29.256817512498845	21.55574540285869
52-53	21.083439100115882	28.147546839920885	29.273258768961778	21.49575529100145
54-55	21.206144865029707	28.436907672731298	29.0386078028822	21.3183396593568
56-57	21.082547108676202	28.18381142138483	29.093851985661534	21.63978948427743
58-59	20.901518647718397	27.842973644031343	29.461864484310635	21.793643223939622
60-61	21.143538416912232	28.16157397541408	29.10813311875137	21.58675448892232
62-63	20.976187404142642	28.194390280224596	29.156995464194846	21.672426851437915
64-65	20.97396097522582	27.98805618333718	29.25203314841871	21.785949693018296
66-67	20.925187043826426	28.010506881672036	29.114548492129895	21.94975758237164
68-69	21.274903632569984	28.13599308989182	28.92353834017993	21.665564937358265
70-71	21.28595931899812	27.967758586275682	28.932995548439266	21.813286546286932
72-73	21.054296617039654	28.120114097719316	29.01011842158287	21.815470863658163
74-75	21.156865569729145	28.079687467779774	28.890751389517767	21.872695572973317
76-77	21.207714076932817	28.118200184252135	28.742984536064508	21.93110120275054
78-79	21.225170263384996	28.187513183408853	28.797248338935866	21.790068214270285
80-81	21.17579616493182	28.004718229552978	28.8294281597271	21.990057445788104
82-83	21.029888007918494	28.05019026215858	28.947076645731183	21.972845084191743
84-85	21.102670611165728	27.95465844656414	28.76071614687995	22.181954795390187
86-87	21.17654849396626	28.055152149230544	28.7219760880916	22.0463232687116
88-89	21.018016542271432	27.938586860866128	28.784825872814224	22.258570724048216
90-91	20.923580751306282	27.7954048088406	28.83305391562488	22.447960524228236
92-93	21.004650259044784	27.946376655008343	28.695476141101135	22.353496944845737
94-95	20.87034632528137	27.726346405444218	28.87810643738156	22.525200831892846
96-97	20.91392382177084	27.780202577031954	28.79557828920832	22.510295311988887
98-99	20.84959054516986	27.799412914217182	28.743125681768195	22.607870858844766
100	20.926294755628874	27.73442906009095	28.680633358722478	22.658642825557695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	482.0
1	422.5
2	790.5
3	1236.5
4	825.5
5	493.0
6	1172.0
7	3065.0
8	5176.5
9	6024.0
10	4867.0
11	3332.0
12	3178.0
13	3665.0
14	4283.5
15	4997.0
16	5797.0
17	6652.5
18	7698.0
19	9007.0
20	11011.0
21	13757.0
22	17631.0
23	23547.0
24	31729.5
25	42411.5
26	56280.0
27	72712.0
28	92496.5
29	115117.5
30	136242.0
31	154704.5
32	169783.0
33	184869.0
34	200610.5
35	212647.0
36	222665.0
37	233822.0
38	244197.0
39	249043.0
40	251614.0
41	253259.0
42	251267.5
43	248386.0
44	243597.0
45	238151.5
46	231843.0
47	222139.0
48	212930.0
49	206322.5
50	200759.0
51	191373.5
52	176823.0
53	163775.5
54	154779.0
55	147397.5
56	136986.0
57	123834.0
58	111700.5
59	100122.5
60	87757.0
61	74635.5
62	62976.5
63	52307.5
64	42382.0
65	34033.5
66	27028.5
67	20863.0
68	15909.5
69	12210.0
70	9158.5
71	6693.5
72	4829.0
73	3343.5
74	2360.5
75	1659.0
76	1042.0
77	668.5
78	449.5
79	287.0
80	190.0
81	97.0
82	48.5
83	29.0
84	13.5
85	10.5
86	9.5
87	6.5
88	2.0
89	1.5
90	3.0
91	1.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035912039464034956
2	0.0
3	0.0
4	4.356494880392433E-5
5	0.0
6	0.0
7	0.0
8	5.22779385647092E-4
9	0.0
10-11	0.0
12-13	0.04324547251269555
14-15	1.1617319681046487E-4
16-17	1.4521649601308109E-5
18-19	6.53474232058865E-5
20-21	7.98690728071946E-5
22-23	0.0
24-25	1.30694846411773E-4
26-27	9.439072240850272E-5
28-29	3.630412400327028E-4
30-31	7.2608248006540545E-6
32-33	2.541288680228919E-4
34-35	1.0165154720915678E-4
36-37	9.439072240850272E-5
38-39	3.339979408300865E-4
40-41	1.7425979521569731E-4
42-43	5.22779385647092E-4
44-45	2.976938168268163E-4
46-47	1.1617319681046487E-4
48-49	8.858206256797947E-4
50-51	4.937360864444758E-4
52-53	7.260824800654056E-4
54-55	4.719536120425136E-4
56-57	5.590835096503622E-4
58-59	1.7425979521569731E-4
60-61	1.815206200163514E-4
62-63	8.930814504804488E-4
64-65	4.211278384379352E-4
66-67	0.001691772178552395
68-69	0.001829727849764822
70-71	4.8647526164382173E-4
72-73	9.802113480882973E-4
74-75	9.874721728889515E-4
76-77	7.84169078470638E-4
78-79	0.0035941082763237577
80-81	0.0030858505402779736
82-83	0.0016482072297484705
84-85	0.0032601103354936707
86-87	0.001735337127356319
88-89	0.002236334038601449
90-91	0.0032455886858923626
92-93	0.002933373219464238
94-95	0.004167713435575428
96-97	0.0035941082763237577
98-99	0.0032455886858923626
100	0.0018152062001635138
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6886270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.780854593181594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34324320617078	43.69959368887461
2	15.262265902047345	17.942580655014375
3	4.868635733455022	8.58547707346163
4	2.01307366816804	4.73320762295793
5	0.9971920829274674	2.9307901414015674
6	0.585363253655443	2.064489137838736
7	0.38931934617271224	1.6019166714383508
8	0.2760452819406389	1.2980942063109209
9	0.19764486364928288	1.045596059213193
>10	0.9854783487872701	10.251482951757362
>50	0.05671422146092688	2.273304824851391
>100	0.023936713922127238	2.376735506228449
>500	6.672894687489272E-4	0.2494299211244222
>1k	3.459549681330588E-4	0.3495677354945698
>5k	2.4711068710385568E-5	0.12907250328381012
>10k+	4.9422137420771135E-5	0.4686613007486083
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16315	0.23692071324534184	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15950	0.23162031114086437	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8886	0.12903937835722387	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.452164960130811E-5	0.0
3	0.0	0.0	0.0	1.452164960130811E-5	0.0
4	0.0	0.0	0.0	7.260824800654055E-5	0.0
5	0.0	0.0	0.0	7.260824800654055E-5	0.0
6	0.0	0.0	0.0	7.260824800654055E-5	0.0
7	0.0	0.0	0.0	7.260824800654055E-5	0.0
8	0.0	0.0	0.0	7.260824800654055E-5	0.0
9	0.0	0.0	0.0	1.1617319681046488E-4	0.0
10-11	0.0	0.0	0.0	1.3795567121242705E-4	0.0
12-13	0.0	0.0	0.0	1.6699897041504326E-4	0.0
14-15	0.0	1.452164960130811E-5	0.0	2.3234639362092977E-4	0.0
16-17	0.0	4.3564948803924335E-5	0.0	2.904329920261622E-4	0.0
18-19	0.0	6.53474232058865E-5	0.0	3.339979408300865E-4	0.0
20-21	0.0	7.260824800654055E-5	0.0	3.848237144346649E-4	0.0
22-23	0.0	7.260824800654055E-5	0.0	7.188216552647514E-4	0.0
24-25	0.0	9.439072240850272E-5	0.0	0.0012851659897157677	0.0
26-27	0.0	1.1617319681046488E-4	0.0	0.0022072907393988327	0.0
28-29	0.0	1.2343402161111894E-4	0.0	0.006135396956552677	0.0
30-31	0.0	1.30694846411773E-4	0.0	0.01833358262165149	0.0
32-33	0.0	1.30694846411773E-4	0.0	0.047689097290695835	0.0
34-35	0.0	1.3795567121242705E-4	0.0	0.09563232344941455	0.0
36-37	0.0	1.597381456143892E-4	0.0	0.16919900032964147	0.0
38-39	0.0	1.7425979521569731E-4	0.0	0.2638293299565657	0.0
40-41	0.0	1.8878144481700542E-4	0.0	0.3605289946516764	0.0
42-43	0.0	2.0330309441831356E-4	0.0	0.4551012376801955	0.0
44-45	0.0	2.105639192189676E-4	0.0	0.5726975561515886	0.0
46-47	0.0	2.1782474401962166E-4	0.0	0.7127298232570027	0.0
48-49	0.0	2.1782474401962166E-4	0.0	0.8574525831836393	0.0
50-51	0.0	2.3234639362092977E-4	0.0	1.0012604791853934	0.0
52-53	0.0	2.3234639362092977E-4	0.0	1.1400293627754938	0.0
54-55	0.0	2.3234639362092977E-4	0.0	1.2924485969908237	0.0
56-57	0.0	2.3960721842158382E-4	0.0	1.4736715231903483	0.0
58-59	0.0	2.468680432222379E-4	0.0	1.666039815458877	0.0
60-61	0.0	2.468680432222379E-4	0.0	1.8633817727158535	0.0
62-63	0.0	2.468680432222379E-4	0.0	2.0539130763098163	0.0
64-65	0.0	2.468680432222379E-4	0.0	2.254674882047901	0.0
66-67	0.0	2.541288680228919E-4	0.0	2.486643712779197	0.0
68-69	0.0	2.61389692823546E-4	0.0	2.7383256828442684	0.0
70-71	0.0	2.61389692823546E-4	0.0	2.999642767419808	0.0
72-73	0.0	2.6865051762420007E-4	0.0	3.2679665479279785	0.0
74-75	0.0	2.759113424248541E-4	0.0	3.5379675789651	0.0
76-77	0.0	2.759113424248541E-4	0.0	3.8256632400414157	0.0
78-79	0.0	2.904329920261622E-4	0.0	4.1448055333293645	0.0
80-81	0.0	2.904329920261622E-4	0.0	4.4834649236814705	0.0
82-83	0.0	3.049546416274703E-4	0.0	4.82749006356126	0.0
84-85	0.0	3.339979408300865E-4	0.0	5.164268900289997	0.0
86-87	0.0	3.339979408300865E-4	0.0	5.521457915533373	0.0
88	0.0	3.6304124003270273E-4	0.0	5.806133073492616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11440	0.0	59.17907	1
GTATCAA	20920	0.0	39.59824	1
ATCAACG	25995	0.0	31.584032	3
TCAACGC	26200	0.0	31.336905	4
TATCAAC	27075	0.0	30.393602	2
CAACGCA	27185	0.0	30.201468	5
AACGCAG	28380	0.0	29.034914	6
GTGGTAT	5140	0.0	28.629442	1
TGGTATC	5155	0.0	27.076485	2
ACGCAGA	32545	0.0	25.088081	7
CGCAGAG	32930	0.0	24.651937	8
GTACATG	18200	0.0	22.990597	1
GCAGAGT	36055	0.0	22.150024	9
TACATGG	18125	0.0	21.728539	2
GAGTACT	25915	0.0	20.69095	12-13
ACATGGG	18665	0.0	20.545969	3
CAGAGTA	34840	0.0	20.533033	10-11
AGAGTAC	33185	0.0	20.18228	10-11
AGTACTT	26940	0.0	19.048576	12-13
GTACTTT	28470	0.0	18.669933	14-15
>>END_MODULE