FastQCFastQC Report
Wed 25 May 2016
SRR2049385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8061517
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82800.047.186261
GTATCAA136350.034.3783381
TCAACGC146950.030.4875354
ATCAACG148100.030.25083
CAACGCA156000.028.7490045
TATCAAC167900.026.9913962
AACGCAG167250.026.7541836
GTACATG217950.024.570371
TACATGG218200.023.5054742
ACGCAGA192100.022.9750467
CGCAGAG194150.022.3935268
ACATGGG223000.022.2195263
CATGGGG155150.018.9983024
GCAGAGT221000.018.673279
GTGGTAT51300.018.2381421
CAGAGTA210250.017.15775710-11
AGAGTAC189850.017.144610-11
GAGTACA166950.016.0803151
CCGGTCG8100.015.6673999
TGGTATC52650.015.3578022
Produced by FastQC (version 0.11.5)