##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049385_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8061517
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01664947180537	31.0	31.0	34.0	28.0	34.0
2	31.115315765010482	31.0	31.0	34.0	28.0	34.0
3	31.28642996101106	31.0	31.0	34.0	28.0	34.0
4	34.87301248635958	37.0	35.0	37.0	32.0	37.0
5	34.91916360159012	37.0	35.0	37.0	32.0	37.0
6	34.8740830044767	37.0	35.0	37.0	32.0	37.0
7	34.847353419958054	37.0	35.0	37.0	32.0	37.0
8	34.828491585392676	37.0	35.0	37.0	32.0	37.0
9	36.4349479136495	39.0	37.0	39.0	32.0	39.0
10-11	36.399074888758534	39.0	37.0	39.0	32.0	39.0
12-13	36.34300479673987	39.0	37.0	39.0	32.0	39.0
14-15	37.56454945142459	40.0	38.0	41.0	32.0	41.0
16-17	37.52803082844085	40.0	38.0	41.0	32.0	41.0
18-19	37.55712479425398	40.0	38.0	41.0	32.0	41.0
20-21	37.64135720361317	40.0	38.0	41.0	32.0	41.0
22-23	37.591684976909434	40.0	38.0	41.0	32.0	41.0
24-25	37.60245739108409	40.0	38.0	41.0	32.0	41.0
26-27	37.5974817766929	40.0	38.0	41.0	32.0	41.0
28-29	37.5349069536168	40.0	38.0	41.0	32.0	41.0
30-31	37.470516653379256	40.0	38.0	41.0	32.0	41.0
32-33	37.42270257570628	40.0	38.0	41.0	32.0	41.0
34-35	37.31768722933909	40.0	37.5	41.0	31.0	41.0
36-37	37.23702964342816	40.0	37.0	41.0	31.0	41.0
38-39	37.13723800371568	40.0	37.0	41.0	31.0	41.0
40-41	37.03791308757396	40.0	37.0	41.0	31.0	41.0
42-43	36.89362814715891	40.0	37.0	41.0	30.0	41.0
44-45	36.76951422418386	40.0	37.0	41.0	30.0	41.0
46-47	36.658244657922324	40.0	36.5	41.0	30.0	41.0
48-49	36.53297202003047	40.0	36.0	41.0	30.0	41.0
50-51	36.39662497765619	40.0	36.0	41.0	30.0	41.0
52-53	36.26538199944254	39.5	36.0	41.0	30.0	41.0
54-55	36.13119255346109	39.0	35.0	41.0	29.0	41.0
56-57	36.01638041574557	39.0	35.0	41.0	29.0	41.0
58-59	35.81043319762273	39.0	35.0	41.0	28.5	41.0
60-61	35.60067131285588	39.0	35.0	40.5	28.0	41.0
62-63	35.40154526747261	38.5	35.0	40.0	28.0	41.0
64-65	35.16145881724246	38.0	34.5	40.0	28.0	41.0
66-67	34.90177562113929	37.5	34.0	40.0	28.0	41.0
68-69	34.61820101601225	37.0	34.0	40.0	28.0	41.0
70-71	34.33339612135036	36.5	34.0	39.5	27.0	41.0
72-73	33.989502906214796	36.0	34.0	39.0	26.0	41.0
74-75	33.639774126879594	36.0	34.0	39.0	26.0	40.5
76-77	33.30269011403189	35.0	33.0	38.5	26.0	40.0
78-79	32.9888702709428	35.0	33.0	37.0	26.0	39.0
80-81	32.63160147153445	35.0	33.0	37.0	25.5	39.0
82-83	32.31904745719695	35.0	33.0	36.5	25.0	39.0
84-85	32.0266382245426	35.0	33.0	36.0	25.0	37.5
86-87	31.704266331014374	35.0	32.5	36.0	24.5	37.0
88-89	31.456170532171548	35.0	32.0	35.0	24.0	37.0
90-91	31.254096096305446	35.0	32.0	35.0	23.5	36.0
92-93	31.01331368525304	35.0	32.0	35.0	21.0	36.0
94-95	30.81271670083931	35.0	32.0	35.0	20.0	36.0
96-97	30.55896098711942	35.0	32.0	35.0	18.0	36.0
98-99	30.34919066721561	35.0	32.0	35.0	6.0	35.0
100	30.226776424338	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3806931622130847
1101	2	0.3934918517022261
1101	3	0.403235722612866
1101	4	0.42875898135217483
1101	5	0.4411907004877662
1101	6	0.5025711963187476
1101	7	0.4430757793164659
1101	8	0.47996739045781567
1101	9	0.6296303399307632
1101	10-11	0.6222741446941384
1101	12-13	0.6722216749911212
1101	14-15	0.7373244557390279
1101	16-17	0.75153911692788
1101	18-19	0.7066455469502628
1101	20-21	0.6481104959851081
1101	22-23	0.6745606476918837
1101	24-25	0.7063183515270026
1101	26-27	0.6663895620344533
1101	28-29	0.7023695897158291
1101	30-31	0.6503609511392909
1101	32-33	0.6562982103296164
1101	34-35	0.6675581695819375
1101	36-37	0.6838023515672305
1101	38-39	0.7249659684530272
1101	40-41	0.7316766121009834
1101	42-43	0.7106157047386716
1101	44-45	0.6587732270911175
1101	46-47	0.6195609024484341
1101	48-49	0.5475887916410116
1101	50-51	0.6289348392154892
1101	52-53	0.6036551554350069
1101	54-55	0.6133352792403741
1101	56-57	0.5355284924593136
1101	58-59	0.6651696226851342
1101	60-61	0.6479283271392902
1101	62-63	0.648436867028849
1101	64-65	0.6262211593311235
1101	66-67	0.6300017660417154
1101	68-69	0.5983371275246654
1101	70-71	0.6070750696725469
1101	72-73	0.6718452899856047
1101	74-75	0.6143959403605237
1101	76-77	0.6275683829173602
1101	78-79	0.5890163211145563
1101	80-81	0.7073812437599827
1101	82-83	0.7215735771162812
1101	84-85	0.6184098432070186
1101	86-87	0.6477050257405352
1101	88-89	0.6723858369296565
1101	90-91	0.6909119399875969
1101	92-93	0.7215153258326588
1101	94-95	0.7040029010239373
1101	96-97	0.6910725983554471
1101	98-99	0.7855317791709844
1101	100	0.7778451320770614
1102	1	0.16225753744684468
1102	2	0.21379619077774592
1102	3	0.2519287135629398
1102	4	0.23449813927800278
1102	5	0.20406675787451434
1102	6	0.27324881353718666
1102	7	0.37153557292251804
1102	8	0.3459221848801519
1102	9	0.4553038930179909
1102	10-11	0.3678429762197055
1102	12-13	0.4335082942817934
1102	14-15	0.47827563520087324
1102	16-17	0.4435117408363638
1102	18-19	0.46368732155466574
1102	20-21	0.4208041382327252
1102	22-23	0.4090397219510251
1102	24-25	0.4949835111683285
1102	26-27	0.45145765458780573
1102	28-29	0.4553797072862693
1102	30-31	0.44852279339572476
1102	32-33	0.5197405424239818
1102	34-35	0.5421795834504266
1102	36-37	0.47034966263263556
1102	38-39	0.4683997403900477
1102	40-41	0.49618381135744016
1102	42-43	0.4740195852281701
1102	44-45	0.4500705314621527
1102	46-47	0.36931723165256614
1102	48-49	0.36707885762591985
1102	50-51	0.4690635223074082
1102	52-53	0.49229073459314066
1102	54-55	0.42370686435521066
1102	56-57	0.39221675243241094
1102	58-59	0.4984556131367981
1102	60-61	0.4277958995184008
1102	62-63	0.46295703177084846
1102	64-65	0.4110939878714319
1102	66-67	0.5390528367524183
1102	68-69	0.4939456026850948
1102	70-71	0.5102987592817527
1102	72-73	0.49065354937132355
1102	74-75	0.4962914191981085
1102	76-77	0.5221038626532817
1102	78-79	0.4922558051210544
1102	80-81	0.441493341223115
1102	82-83	0.48445167736600325
1102	84-85	0.4996667135754862
1102	86-87	0.5076798635423998
1102	88-89	0.5044906336410335
1102	90-91	0.5815451324943943
1102	92-93	0.5903827893742459
1102	94-95	0.5440999262180988
1102	96-97	0.5265077454459011
1102	98-99	0.5863541157397911
1102	100	0.6110491272778447
1103	1	0.23664877131961148
1103	2	0.259102362110319
1103	3	0.32114689550043707
1103	4	0.28806627724705436
1103	5	0.3054420187320943
1103	6	0.36181242341236697
1103	7	0.4178461935995017
1103	8	0.43133634140782817
1103	9	0.4956139690112167
1103	10-11	0.49410966126124833
1103	12-13	0.495568134026648
1103	14-15	0.5938633358479279
1103	16-17	0.588932558255749
1103	18-19	0.5618860589383488
1103	20-21	0.5076051383508471
1103	22-23	0.5007529984534997
1103	24-25	0.5239293348220357
1103	26-27	0.5276184052013946
1103	28-29	0.5134593853322329
1103	30-31	0.5384692437638705
1103	32-33	0.5470737740392693
1103	34-35	0.5446205213564852
1103	36-37	0.5173494066969013
1103	38-39	0.48563405940409865
1103	40-41	0.5015450909048909
1103	42-43	0.49423022362377367
1103	44-45	0.3878016611231345
1103	46-47	0.4085532306550661
1103	48-49	0.42677173473739316
1103	50-51	0.355862865405669
1103	52-53	0.3648933549813762
1103	54-55	0.38445265454039657
1103	56-57	0.40103603554984346
1103	58-59	0.4491903619916542
1103	60-61	0.41631411361193216
1103	62-63	0.4229878753087988
1103	64-65	0.42124646651064523
1103	66-67	0.44468621208547177
1103	68-69	0.4417241953010205
1103	70-71	0.4010970326998802
1103	72-73	0.4401302264066729
1103	74-75	0.419818492605728
1103	76-77	0.4800343925058854
1103	78-79	0.3767056089036558
1103	80-81	0.3673187339860391
1103	82-83	0.4119633421295603
1103	84-85	0.49984345689161813
1103	86-87	0.4346736685849919
1103	88-89	0.3937748174685183
1103	90-91	0.43754682560775393
1103	92-93	0.4035511589979528
1103	94-95	0.4595288810815319
1103	96-97	0.4624849934148223
1103	98-99	0.4733873283239518
1103	100	0.48989439771661125
1104	1	0.20874001559640476
1104	2	0.18671013611493592
1104	3	0.2575317474063823
1104	4	0.31735954140910394
1104	5	0.3543362413167017
1104	6	0.35615678950649254
1104	7	0.41293853398436653
1104	8	0.38956812794451423
1104	9	0.47345026064166973
1104	10-11	0.47964894941819836
1104	12-13	0.4656052023407611
1104	14-15	0.5967230728417263
1104	16-17	0.5634200455997345
1104	18-19	0.5202206989400224
1104	20-21	0.48445956205784313
1104	22-23	0.5247694691945881
1104	24-25	0.5918552751005706
1104	26-27	0.5194531280831711
1104	28-29	0.5512604603842703
1104	30-31	0.4780507664282254
1104	32-33	0.498194204806758
1104	34-35	0.5437534535799671
1104	36-37	0.5142481227272526
1104	38-39	0.5692811798057491
1104	40-41	0.6092098020490511
1104	42-43	0.5761369551310622
1104	44-45	0.46485663975350633
1104	46-47	0.42983307122660364
1104	48-49	0.44566242558413904
1104	50-51	0.48885192896501195
1104	52-53	0.446658779641119
1104	54-55	0.4844495558697659
1104	56-57	0.4770865595611795
1104	58-59	0.5672083663880301
1104	60-61	0.48381669334856525
1104	62-63	0.4801439751352703
1104	64-65	0.558388028718511
1104	66-67	0.5883405826297263
1104	68-69	0.5877974889414972
1104	70-71	0.5900178478518541
1104	72-73	0.5541754923846085
1104	74-75	0.5897149634065428
1104	76-77	0.6156180327196097
1104	78-79	0.5665600385839937
1104	80-81	0.5899059522310068
1104	82-83	0.5108825220794415
1104	84-85	0.5605121612142341
1104	86-87	0.5620684183169402
1104	88-89	0.5440119031053214
1104	90-91	0.5629690661482236
1104	92-93	0.554819019287482
1104	94-95	0.6119967295737112
1104	96-97	0.6033249870233455
1104	98-99	0.6181402336076722
1104	100	0.5800007530527438
1105	1	0.10945761731842651
1105	2	0.09754184035054436
1105	3	0.1088190570575378
1105	4	0.1560267128682824
1105	5	0.1211634570878104
1105	6	0.1518190498291503
1105	7	0.11191535293247057
1105	8	0.16115325866933006
1105	9	0.25832904476619234
1105	10-11	0.2213083654610628
1105	12-13	0.22275637042187668
1105	14-15	0.23047587887159438
1105	16-17	0.25128674789532823
1105	18-19	0.20673204340032925
1105	20-21	0.2127488396424937
1105	22-23	0.2037006577277296
1105	24-25	0.18502985852546772
1105	26-27	0.18164748219310667
1105	28-29	0.23903679612312345
1105	30-31	0.2442421992260364
1105	32-33	0.2525013223574959
1105	34-35	0.3009611830348504
1105	36-37	0.2148138444403216
1105	38-39	0.24315938464950904
1105	40-41	0.2589912272465824
1105	42-43	0.23961673449910847
1105	44-45	0.24382902756762803
1105	46-47	0.24640047593636183
1105	48-49	0.2583743606374824
1105	50-51	0.28047363791840496
1105	52-53	0.2791077350720599
1105	54-55	0.27662230187831227
1105	56-57	0.2693551892270065
1105	58-59	0.2993335375364836
1105	60-61	0.306429767876935
1105	62-63	0.21681585095063838
1105	64-65	0.3005649946433593
1105	66-67	0.3344920056511782
1105	68-69	0.2703665914391138
1105	70-71	0.2922373611234832
1105	72-73	0.25018515402572206
1105	74-75	0.2720872178281226
1105	76-77	0.3151027207392616
1105	78-79	0.24925460704733382
1105	80-81	0.232952853975263
1105	82-83	0.20838431535898394
1105	84-85	0.239611029667266
1105	86-87	0.28394309032309195
1105	88-89	0.23695122434753912
1105	90-91	0.27486126951783874
1105	92-93	0.2675118974177195
1105	94-95	0.252733334379041
1105	96-97	0.21967509044901234
1105	98-99	0.2133162968579576
1105	100	0.25372114367647924
1106	1	0.12757771878740698
1106	2	0.09962925079240037
1106	3	0.14716723647726226
1106	4	0.15387153959740374
1106	5	0.15114431192264277
1106	6	0.17620050203358772
1106	7	0.19600437934239068
1106	8	0.1798949715352549
1106	9	0.29082371449746347
1106	10-11	0.2695404231677827
1106	12-13	0.27715368091789117
1106	14-15	0.338338672195448
1106	16-17	0.3524030989594493
1106	18-19	0.30767158759439184
1106	20-21	0.324286299974041
1106	22-23	0.34712036577744954
1106	24-25	0.38519878411406694
1106	26-27	0.33502639449162075
1106	28-29	0.40708087852068076
1106	30-31	0.3931007229377457
1106	32-33	0.37927396444540307
1106	34-35	0.43877134036335264
1106	36-37	0.28457334051098826
1106	38-39	0.339828180450489
1106	40-41	0.4020127026236011
1106	42-43	0.37756340459874593
1106	44-45	0.37107549104674575
1106	46-47	0.32644199064679214
1106	48-49	0.332587199720777
1106	50-51	0.37171173510975564
1106	52-53	0.3606576580261063
1106	54-55	0.36603166197166104
1106	56-57	0.27079628203819084
1106	58-59	0.3786992703553267
1106	60-61	0.36901614737502086
1106	62-63	0.30370806552851803
1106	64-65	0.34646154274718555
1106	66-67	0.3931281715550341
1106	68-69	0.4021970732417728
1106	70-71	0.36088540334726815
1106	72-73	0.35318824040881935
1106	74-75	0.38300511200425547
1106	76-77	0.4598643557917086
1106	78-79	0.34109324538687247
1106	80-81	0.403660416079731
1106	82-83	0.36203322084513445
1106	84-85	0.37762520694073487
1106	86-87	0.3956733927864775
1106	88-89	0.3880865776939757
1106	90-91	0.453313586103274
1106	92-93	0.36938451204825284
1106	94-95	0.4195924975695995
1106	96-97	0.4054063222474298
1106	98-99	0.43518777041414936
1106	100	0.40031030856629357
1107	1	0.18447251210526971
1107	2	0.15773051154523188
1107	3	0.21344774993738724
1107	4	0.193256162718491
1107	5	0.18501573976817554
1107	6	0.2469623857312513
1107	7	0.27617535642728797
1107	8	0.3397292698885579
1107	9	0.35308629799561686
1107	10-11	0.32600133506677764
1107	12-13	0.32513812647976437
1107	14-15	0.3281314620168416
1107	16-17	0.4076705508417504
1107	18-19	0.34795415131683427
1107	20-21	0.2909388477255064
1107	22-23	0.28927226216222124
1107	24-25	0.35043928360224186
1107	26-27	0.34871409175869417
1107	28-29	0.3830143920944664
1107	30-31	0.3688331090470456
1107	32-33	0.37009360038872074
1107	34-35	0.3590127938671728
1107	36-37	0.3352081288007014
1107	38-39	0.37501004518015435
1107	40-41	0.4437503856910894
1107	42-43	0.41277961001908636
1107	44-45	0.32789981057344164
1107	46-47	0.37360411289538575
1107	48-49	0.36844572680747234
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2216	3	-0.956748864727782
2216	4	-1.0935940891779339
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2216	12-13	-1.573769347271714
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2216	36-37	-2.203423479898184
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2216	54-55	-2.171957135374356
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2216	68-69	-3.0661121185747042
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2216	72-73	-3.3286759314512366
2216	74-75	-3.787912085367317
2216	76-77	-4.494921935043422
2216	78-79	-3.4336629058150763
2216	80-81	-3.3173163645445456
2216	82-83	-3.593278993376522
2216	84-85	-3.6011379130593575
2216	86-87	-3.251390668432471
2216	88-89	-3.190463893210037
2216	90-91	-3.3770373584417612
2216	92-93	-3.48075802345603
2216	94-95	-3.2983094625112273
2216	96-97	-3.6395456343445325
2216	98-99	-3.3839685003456204
2216	100	-2.9696041696914577
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105381.0
3	18406.0
4	11782.0
5	10040.0
6	9360.0
7	9760.0
8	10698.0
9	12522.0
10	14404.0
11	16068.0
12	18315.0
13	20610.0
14	23667.0
15	24805.0
16	24754.0
17	26311.0
18	24950.0
19	23216.0
20	22994.0
21	24648.0
22	27033.0
23	31669.0
24	38832.0
25	47737.0
26	58789.0
27	69602.0
28	85299.0
29	106848.0
30	131175.0
31	157204.0
32	202963.0
33	272936.0
34	377512.0
35	553054.0
36	905861.0
37	1597045.0
38	2144973.0
39	797990.0
40	2304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90392732886997	20.606323934718464	14.445913154644764	23.043835581766807
2	15.2039662844477	20.31270069039668	41.0475345306927	23.435798494462915
3	18.04491291505057	25.474503952224335	30.77466922909101	25.70591390363408
4	12.416421180288001	17.039015425153202	39.359401862515305	31.185161532043498
5	12.79238308182761	37.92210944858211	35.985525105252385	13.299982364337886
6	30.399418592457117	38.2864586326076	17.92232684143725	13.391795933498038
7	26.402526524072307	32.82666918511581	23.065440751146486	17.705363539665388
8	24.344318435803412	36.93218082647455	20.85347657637902	17.870024161343018
9	24.460520043748378	17.32119005214635	22.1492227688632	36.06906713524208
10-11	23.470832728591148	27.303602335687543	29.411263549922147	19.814301385799162
12-13	23.79994683391232	24.915739693671995	29.398910467514085	21.885403004901605
14-15	21.34475358522048	27.261860476804927	26.480603059789402	24.912782878185187
16-17	20.867034936490953	30.526157010814543	27.195205096111774	21.41160295658273
18-19	20.773610201208818	29.462237713899086	29.21958905909905	20.544563025793046
20-21	21.873975980302337	28.72395379430769	28.71566390790085	20.686406317489123
22-23	21.538183169308414	28.56308983879467	28.755254766675577	21.14347222522134
24-25	21.400566230812398	28.696974187032882	28.67755297152043	21.22490661063429
26-27	21.14253959612477	28.676070785726328	28.77029150345055	21.41109811469835
28-29	21.197930417630108	28.66694237105985	28.922491180510164	21.212636030799885
30-31	21.487800324255787	29.016016031978577	28.45812084903665	21.038062794728983
32-33	21.11635190076938	28.796512801064463	28.852493336566496	21.23464196159966
34-35	21.335413126523843	28.518437119679607	28.829642897962103	21.316506855834444
36-37	21.571444747957493	28.781109182484766	28.371220758431022	21.276225311126726
38-39	21.385152896194317	28.94512834777351	28.492933332150294	21.17678542388187
40-41	21.29646371154237	28.669602125821097	28.687521647067086	21.346412515569448
42-43	21.21687634873062	28.675231406636026	28.66175457106403	21.446137673569325
44-45	21.321574559650948	28.719109796261776	28.60414872468164	21.355166919405637
46-47	21.338893701636998	28.854989520949353	28.422164833251617	21.383951944162032
48-49	21.264065748628195	28.6967548782182	28.510428712123844	21.528750661029765
50-51	21.28806200688455	28.603584290593176	28.517307119799373	21.591046582722896
52-53	21.279083201512776	28.60239928927964	28.52996468363128	21.588552825576304
54-55	21.343256772079936	28.87489238663562	28.373928326009164	21.40792251527528
56-57	21.27639138797543	28.679363405100204	28.37479059378296	21.669454613141404
58-59	21.180776274279317	28.330024685337186	28.662192468691938	21.827006571691562
60-61	21.39647831455868	28.677450101886574	28.344210706969104	21.581860876585644
62-63	21.228555847788066	28.76611124840641	28.34464742432535	21.660685479480172
64-65	21.282981982990346	28.49399246877729	28.439039559393937	21.783985988838424
66-67	21.217701530968373	28.512717740309295	28.33394034342087	21.935640385301465
68-69	21.525685573960434	28.60522863902516	28.2668911412596	21.602194645754807
70-71	21.521896081965366	28.452422730587156	28.30087952637909	21.724801661068387
72-73	21.307115291548264	28.5794482981126	28.44893717348952	21.664499236849615
74-75	21.40354645611565	28.507173862368884	28.372703690543265	21.7165759909722
76-77	21.456188877182925	28.580871047840994	28.165350676360696	21.79758939861539
78-79	21.51777045580442	28.598919358137152	28.27381720568877	21.609492980369655
80-81	21.50645147319982	28.437192213435004	28.319149841776685	21.73720647158849
82-83	21.3623938574025	28.491437581771343	28.442744716548024	21.703423844278134
84-85	21.456784943788758	28.339441423542095	28.31741519545423	21.88635843721492
86-87	21.58490774887372	28.46143140808929	28.272252738235604	21.681408104801385
88-89	21.48401660885072	28.318275375817098	28.320552481738325	21.877155533593857
90-91	21.46109099970168	28.214243345241343	28.349871265470107	21.974794389586876
92-93	21.547647256238243	28.39047149805105	28.19639265252733	21.865488593183375
94-95	21.48436503861624	28.162810213486754	28.3413368244873	22.01148792340971
96-97	21.53354635362054	28.152331626913394	28.337687559301806	21.97643446016426
98-99	21.5450268231153	28.232018861958615	28.234785090696953	21.988169224229136
100	21.679728517597866	28.110974646583266	28.184980568793687	22.024316267025178
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2654.0
1	2033.0
2	1788.0
3	2264.0
4	2151.0
5	1776.0
6	1522.0
7	1387.0
8	1368.5
9	1381.5
10	1240.0
11	1129.5
12	1218.0
13	1288.5
14	1412.5
15	1627.5
16	1932.5
17	2523.0
18	3410.0
19	4697.5
20	6722.0
21	9939.0
22	14585.0
23	21634.5
24	31263.0
25	44128.5
26	61198.0
27	81413.5
28	105025.0
29	131962.0
30	159242.5
31	183095.5
32	202397.5
33	220714.5
34	238466.0
35	253245.5
36	266035.0
37	279508.0
38	291269.0
39	297166.0
40	300514.0
41	301801.5
42	299146.5
43	295157.0
44	288151.0
45	279638.5
46	272155.0
47	261581.5
48	249483.5
49	240280.0
50	233177.5
51	222879.5
52	208925.0
53	195918.0
54	184839.0
55	175246.5
56	161821.5
57	145482.0
58	132190.0
59	120168.0
60	105697.0
61	89097.5
62	74803.5
63	62618.5
64	51053.5
65	40954.5
66	32252.5
67	24778.5
68	19136.5
69	14853.0
70	11155.0
71	8372.5
72	6259.0
73	4391.0
74	3078.0
75	2144.5
76	1469.0
77	1061.5
78	778.0
79	555.5
80	389.0
81	290.0
82	223.0
83	172.0
84	139.5
85	102.5
86	79.5
87	63.5
88	60.0
89	45.0
90	40.5
91	44.0
92	39.0
93	29.0
94	22.0
95	24.0
96	23.0
97	22.5
98	25.0
99	32.5
100	60.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01904108122577922
2	0.04212606634706594
3	0.04370145222046918
4	0.045946687205398185
5	0.04937036044208552
6	0.04750966846562502
7	0.0452520288675196
8	0.044706225887757856
9	0.0448798904722275
10-11	0.045593155729870695
12-13	0.046504894798336346
14-15	0.04477445126022807
16-17	0.0408732004162492
18-19	0.04428446903976013
20-21	0.043484371489882115
22-23	0.044594584369170215
24-25	0.04117091113248288
26-27	0.04106547192048345
28-29	0.04179734409789125
30-31	0.04183455793742046
32-33	0.041667095659539015
34-35	0.041481026461892966
36-37	0.03851012160614435
38-39	0.039167566104493734
40-41	0.0405134666341335
42-43	0.04025917206401723
44-45	0.038913271534377465
46-47	0.037933307093441594
48-49	0.03805115091861743
50-51	0.03952109758002123
52-53	0.039868426748960524
54-55	0.03913035226496452
56-57	0.03742471795320906
58-59	0.0383240524084983
60-61	0.037784451735324756
62-63	0.037902295560500586
64-65	0.03472051228075311
66-67	0.035874141306158625
68-69	0.03715801876991638
70-71	0.0383178501019101
72-73	0.03494379531792837
74-75	0.035384159085690696
76-77	0.03655639503086082
78-79	0.0368789149734473
80-81	0.036841701133918096
82-83	0.035247708340750264
84-85	0.036500574271567
86-87	0.037182827996269194
88-89	0.03774103558920734
90-91	0.038119376291087644
92-93	0.036426146592508576
94-95	0.03788989094732418
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8061517.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.22634262775415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77829092171207	42.958400456977294
2	15.2597600237334	17.770400311184133
3	4.976727973957453	8.69330004530326
4	2.141095228894491	4.986725775850412
5	1.0772104170344061	3.136101141221563
6	0.6883649356755616	2.404858355854632
7	0.4055704327680382	1.6530418084626828
8	0.3079825426141054	1.4346157639692634
9	0.20731681921535924	1.0864170133316706
>10	1.0741343148539109	11.023878985121863
>50	0.05990375814671049	2.3756334579503733
>100	0.023151437355256	2.248295453859142
>500	4.2715774748928125E-4	0.1504907114391505
>1k	6.403629159300385E-5	0.07784071947456044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.4809226352806797E-5	0.0	0.0	0.0
6	0.0	2.4809226352806797E-5	0.0	0.0	0.0
7	0.0	2.4809226352806797E-5	0.0	0.0	0.0
8	0.0	2.4809226352806797E-5	0.0	0.0	0.0
9	0.0	2.4809226352806797E-5	0.0	0.0	0.0
10-11	0.0	2.4809226352806797E-5	0.0	0.0	0.0
12-13	0.0	2.4809226352806797E-5	0.0	6.202306588201699E-6	0.0
14-15	0.0	4.341614611741189E-5	0.0	4.341614611741189E-5	0.0
16-17	0.0	6.202306588201699E-5	0.0	8.68322922348238E-5	0.0
18-19	0.0	6.202306588201699E-5	0.0	1.488553581168408E-4	0.0
20-21	0.0	6.202306588201699E-5	0.0	1.9847381082245438E-4	0.0
22-23	0.0	7.44276790584204E-5	0.0	3.783407018803037E-4	0.0
24-25	0.0	7.44276790584204E-5	0.0	8.621206157600362E-4	0.0
26-27	0.0	8.68322922348238E-5	0.0	0.0017304435381082742	0.0
28-29	0.0	8.68322922348238E-5	0.0	0.005321579052677059	0.0
30-31	0.0	8.68322922348238E-5	0.0	0.016113592516148016	0.0
32-33	0.0	9.923690541122719E-5	0.0	0.04156165644753959	0.0
34-35	0.0	1.2404613176403398E-4	0.0	0.08154172471508775	0.0
36-37	0.0	1.3024843835223569E-4	0.0	0.14203282086981892	0.0
38-39	0.0	1.426530515286391E-4	0.0	0.22342569022678982	0.0
40-41	0.0	1.6125997129324418E-4	0.0	0.3072436614597476	0.0
42-43	0.0	1.736645844696476E-4	0.0	0.3911670719047048	0.0
44-45	0.0	1.736645844696476E-4	0.0	0.4951363372427299	0.0
46-47	0.0	1.736645844696476E-4	0.0	0.6179171984627707	0.0
48-49	0.0	1.798668910578493E-4	0.0	0.7468817593512487	0.0
50-51	0.0	1.86069197646051E-4	0.0	0.8727389646390376	0.0
52-53	0.0	2.0467611741065608E-4	0.0	0.9967540848701305	0.0
54-55	0.0	2.1708073058705947E-4	0.0	1.1347430018444418	0.0
56-57	0.0	2.2328303717526117E-4	0.0	1.2961890423353322	0.0
58-59	0.0	2.2328303717526117E-4	0.0	1.4703001928793302	0.0
60-61	0.0	2.2328303717526117E-4	0.0	1.6460797638955547	0.0
62-63	0.0	2.2328303717526117E-4	0.0	1.8192853776776752	0.0
64-65	0.0	2.2328303717526117E-4	0.0	2.004659916985848	0.0
66-67	0.0	2.2328303717526117E-4	0.0	2.21462660191624	0.0
68-69	0.0	2.2328303717526117E-4	0.0	2.4396152734032563	0.0
70-71	0.0	2.2328303717526117E-4	0.0	2.6755634206316254	0.0
72-73	0.0	2.2948534376346288E-4	0.0	2.917205285307964	6.202306588201699E-6
74-75	0.0	2.3568765035166458E-4	0.0	3.1622398116880484	1.2404613176403399E-5
76-77	0.0	2.3568765035166458E-4	0.0	3.4193440266887736	1.2404613176403399E-5
78-79	0.0	2.3568765035166458E-4	0.0	3.7066348678542758	1.2404613176403399E-5
80-81	0.0	2.3568765035166458E-4	0.0	4.009859434644869	1.2404613176403399E-5
82-83	0.0	2.4188995693986628E-4	0.0	4.318492412780373	1.2404613176403399E-5
84-85	0.0	2.4809226352806796E-4	0.0	4.62404904684813	1.2404613176403399E-5
86-87	0.0	2.4809226352806796E-4	0.0	4.944906771268981	1.2404613176403399E-5
88	0.0	2.4809226352806796E-4	0.0	5.205422254893216	1.2404613176403399E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8280	0.0	47.18626	1
GTATCAA	13635	0.0	34.378338	1
TCAACGC	14695	0.0	30.487535	4
ATCAACG	14810	0.0	30.2508	3
CAACGCA	15600	0.0	28.749004	5
TATCAAC	16790	0.0	26.991396	2
AACGCAG	16725	0.0	26.754183	6
GTACATG	21795	0.0	24.57037	1
TACATGG	21820	0.0	23.505474	2
ACGCAGA	19210	0.0	22.975046	7
CGCAGAG	19415	0.0	22.393526	8
ACATGGG	22300	0.0	22.219526	3
CATGGGG	15515	0.0	18.998302	4
GCAGAGT	22100	0.0	18.67327	9
GTGGTAT	5130	0.0	18.238142	1
CAGAGTA	21025	0.0	17.157757	10-11
AGAGTAC	18985	0.0	17.1446	10-11
GAGTACA	16695	0.0	16.080315	1
CCGGTCG	810	0.0	15.667399	9
TGGTATC	5265	0.0	15.357802	2
>>END_MODULE