FastQCFastQC Report
Wed 25 May 2016
SRR2049385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8061517
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187190.23220195404909522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183100.22712846725994623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107930.1338829900129219No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127750.058.88221
GTATCAA241500.041.6407551
TCAACGC295150.033.5653464
ATCAACG296000.033.4530833
CAACGCA305100.032.5169145
TATCAAC311300.032.0051652
AACGCAG318100.031.242576
ACGCAGA364900.027.132467
CGCAGAG366150.026.872848
GTGGTAT58300.026.1277141
GCAGAGT397050.024.4259629
TGGTATC58600.023.6585272
GTACATG214000.023.0894681
TACATGG215600.021.645312
CAGAGTA391150.021.1990910-11
GAGTACT286000.021.18130712-13
AGAGTAC370650.020.9503510-11
ACATGGG222100.019.9326783
GTACTTT312700.019.2597814-15
AGTACTT302100.019.05646912-13
Produced by FastQC (version 0.11.5)