##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049385_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8061517 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.865699842845956 31.0 31.0 34.0 30.0 34.0 2 32.048848002181224 33.0 31.0 34.0 30.0 34.0 3 32.0222407271485 34.0 31.0 34.0 30.0 34.0 4 35.70822340261765 37.0 35.0 37.0 33.0 37.0 5 35.80170680034539 37.0 35.0 37.0 35.0 37.0 6 35.851445949937215 37.0 35.0 37.0 35.0 37.0 7 35.78774863341478 37.0 35.0 37.0 35.0 37.0 8 35.794631581127966 37.0 35.0 37.0 35.0 37.0 9 37.5416913466783 39.0 37.0 39.0 35.0 39.0 10-11 37.50403298783591 39.0 37.0 39.0 35.0 39.0 12-13 37.45175250514264 39.0 37.0 39.0 34.5 39.0 14-15 38.74308985517242 40.0 38.0 41.0 34.5 41.0 16-17 38.77065557264222 40.0 38.0 41.0 35.0 41.0 18-19 38.842541918599196 40.0 38.0 41.0 35.0 41.0 20-21 38.8723758195883 40.0 38.0 41.0 35.0 41.0 22-23 38.840325462316834 40.0 38.0 41.0 35.0 41.0 24-25 38.82127079803963 40.0 38.0 41.0 35.0 41.0 26-27 38.705693729852584 40.0 38.0 41.0 34.5 41.0 28-29 38.62849727911012 40.0 38.0 41.0 34.0 41.0 30-31 38.527633260588544 40.0 38.0 41.0 34.0 41.0 32-33 38.39089292995351 40.0 38.0 41.0 34.0 41.0 34-35 38.29084420463295 40.0 38.0 41.0 34.0 41.0 36-37 38.178982938322896 40.0 38.0 41.0 34.0 41.0 38-39 38.089039755172635 40.0 38.0 41.0 33.5 41.0 40-41 37.9827394149265 40.0 38.0 41.0 33.0 41.0 42-43 37.85343924722853 40.0 37.5 41.0 33.0 41.0 44-45 37.681066789290405 40.0 37.0 41.0 33.0 41.0 46-47 37.46245836856761 40.0 37.0 41.0 33.0 41.0 48-49 37.266926001644606 40.0 37.0 41.0 32.0 41.0 50-51 37.12869897812037 40.0 37.0 41.0 31.5 41.0 52-53 36.99645662224616 40.0 36.5 41.0 31.0 41.0 54-55 36.85994255175546 40.0 36.0 41.0 31.0 41.0 56-57 36.725654551122325 39.5 36.0 41.0 31.0 41.0 58-59 36.53873309452799 39.0 35.5 41.0 31.0 41.0 60-61 36.3665896257491 39.0 35.0 41.0 31.0 41.0 62-63 36.16753596128372 39.0 35.0 41.0 30.0 41.0 64-65 35.94731810402434 38.5 35.0 40.5 30.0 41.0 66-67 35.682759088642996 38.0 35.0 40.0 30.0 41.0 68-69 35.39928452672121 37.0 35.0 40.0 29.0 41.0 70-71 35.07258162452551 37.0 34.5 40.0 29.0 41.0 72-73 34.77306119927552 36.0 34.0 39.0 29.0 41.0 74-75 34.46514539385081 36.0 34.0 39.0 29.0 41.0 76-77 34.13601373041823 35.5 34.0 38.5 29.0 40.0 78-79 33.79483452059954 35.0 34.0 37.0 29.0 39.5 80-81 33.46959864998114 35.0 34.0 37.0 28.0 39.0 82-83 33.16417108591348 35.0 34.0 36.5 28.0 39.0 84-85 32.883257084243574 35.0 34.0 36.0 28.0 37.5 86-87 32.64031236304532 35.0 33.0 36.0 27.0 37.0 88-89 32.40658457955246 35.0 33.0 35.5 27.0 37.0 90-91 32.20664907113637 35.0 33.0 35.0 27.0 36.0 92-93 32.01715247886967 35.0 33.0 35.0 26.0 36.0 94-95 31.85567635719183 35.0 33.0 35.0 26.0 36.0 96-97 31.64717149390121 35.0 33.0 35.0 25.5 36.0 98-99 31.494700749251038 35.0 33.0 35.0 25.0 35.5 100 31.37804931751679 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22278130670789764 1101 2 0.24277923388207512 1101 3 0.398004442132077 1101 4 0.21444961963290154 1101 5 0.21970218660068497 1101 6 0.18921062604327687 1101 7 0.04914471547287036 1101 8 0.13361705677139923 1101 9 0.27118257594550954 1101 10-11 0.2772108075531392 1101 12-13 0.19416785530127356 1101 14-15 0.3424630865594125 1101 16-17 0.25718260261994885 1101 18-19 0.3227055615277621 1101 20-21 0.3461138510461055 1101 22-23 0.3148989052000388 1101 24-25 0.33000971621502373 1101 26-27 0.3677143700986676 1101 28-29 0.3616381956608521 1101 30-31 0.35987890935822264 1101 32-33 0.4050688323222573 1101 34-35 0.40034396066787536 1101 36-37 0.4495219206800911 1101 38-39 0.41328475761097394 1101 40-41 0.34746448839813127 1101 42-43 0.40196275543831206 1101 44-45 0.42238272394796184 1101 46-47 0.4102211166397538 1101 48-49 0.45303529178509194 1101 50-51 0.4307666535984467 1101 52-53 0.4795287300805313 1101 54-55 0.4224830900210108 1101 56-57 0.3936774227102049 1101 58-59 0.4036621057230505 1101 60-61 0.4179994266435898 1101 62-63 0.4066536010049333 1101 64-65 0.3318741640132927 1101 66-67 0.3303719143305983 1101 68-69 0.36831612245502754 1101 70-71 0.36566506353412365 1101 72-73 0.2865035106744003 1101 74-75 0.3777667445779045 1101 76-77 0.30450315495157554 1101 78-79 0.3309959210623745 1101 80-81 0.4084952206583594 1101 82-83 0.3938823953967656 1101 84-85 0.434643961351739 1101 86-87 0.3447903444333136 1101 88-89 0.4104096741216736 1101 90-91 0.46393386164522354 1101 92-93 0.4557475813890761 1101 94-95 0.5036973745518267 1101 96-97 0.556308171415651 1101 98-99 0.5718830360345244 1101 100 0.6182900795351323 1102 1 0.20341621815648026 1102 2 0.17103834319382116 1102 3 0.3727690896323992 1102 4 0.20662472883753225 1102 5 0.162369362327496 1102 6 0.14571255359031454 1102 7 0.14131331170208483 1102 8 0.16403458686799866 1102 9 0.13504028729977335 1102 10-11 0.21600421839145412 1102 12-13 0.21418126563859374 1102 14-15 0.24317440453859973 1102 16-17 0.19536586686449198 1102 18-19 0.22393592218593739 1102 20-21 0.22110556730150677 1102 22-23 0.21296725084381762 1102 24-25 0.2243513744669272 1102 26-27 0.2593905802034584 1102 28-29 0.261990077729358 1102 30-31 0.2705036712761526 1102 32-33 0.28876658275436995 1102 34-35 0.23635583432306362 1102 36-37 0.2842587325527077 1102 38-39 0.2703344618061365 1102 40-41 0.2695158726645488 1102 42-43 0.33016736824883 1102 44-45 0.3260162645620568 1102 46-47 0.3452586137568545 1102 48-49 0.3014275283874923 1102 50-51 0.3669434808810834 1102 52-53 0.36641017665210285 1102 54-55 0.2513379551877435 1102 56-57 0.3315108261544779 1102 58-59 0.3421053232020128 1102 60-61 0.29208477253527576 1102 62-63 0.3181064782457099 1102 64-65 0.2619793649783162 1102 66-67 0.2583728714097475 1102 68-69 0.2984497507437993 1102 70-71 0.31791545634255414 1102 72-73 0.30026335895834677 1102 74-75 0.26426421928830024 1102 76-77 0.2664565465792066 1102 78-79 0.2863186027152196 1102 80-81 0.2947640199645676 1102 82-83 0.33715469046045854 1102 84-85 0.3478703157526226 1102 86-87 0.36767189984241355 1102 88-89 0.33593579555843434 1102 90-91 0.35422734720135907 1102 92-93 0.3830215513412867 1102 94-95 0.3725089379337092 1102 96-97 0.4321274112978628 1102 98-99 0.4296130049640041 1102 100 0.45456109014333634 1103 1 0.21494152954496215 1103 2 0.20777296408080304 1103 3 0.34521724623881767 1103 4 0.22407934946201635 1103 5 0.22533063728123182 1103 6 0.14123610624088911 1103 7 0.17673803378212227 1103 8 0.1570774331359317 1103 9 0.22512779763563628 1103 10-11 0.20422156033473016 1103 12-13 0.2230026651477246 1103 14-15 0.2909561799451339 1103 16-17 0.28647712016213234 1103 18-19 0.27977552266367667 1103 20-21 0.3044129461257796 1103 22-23 0.3358657231043196 1103 24-25 0.3016903154179644 1103 26-27 0.3042819059505888 1103 28-29 0.3620946832601888 1103 30-31 0.3103951282723827 1103 32-33 0.3569200063870852 1103 34-35 0.43311176678227525 1103 36-37 0.3691371138575832 1103 38-39 0.3730089410753479 1103 40-41 0.42936375496035595 1103 42-43 0.4329402679351446 1103 44-45 0.4657974230978468 1103 46-47 0.4179853793853354 1103 48-49 0.44331154355989355 1103 50-51 0.40723083437427476 1103 52-53 0.4010299316100401 1103 54-55 0.377914059977023 1103 56-57 0.349961857116142 1103 58-59 0.4711046800814316 1103 60-61 0.41031645344246925 1103 62-63 0.4081471490598503 1103 64-65 0.4502420534848355 1103 66-67 0.4137311978524991 1103 68-69 0.4037243402622437 1103 70-71 0.4561328803165168 1103 72-73 0.43681779124037945 1103 74-75 0.42442872362607886 1103 76-77 0.4292637186861441 1103 78-79 0.4086815294388444 1103 80-81 0.4265725176873971 1103 82-83 0.45101505454545787 1103 84-85 0.4292042167383059 1103 86-87 0.3891221859654408 1103 88-89 0.48138429013129524 1103 90-91 0.4674621780868975 1103 92-93 0.5010448414936661 1103 94-95 0.45315054914762953 1103 96-97 0.5456429511910343 1103 98-99 0.5550779548924716 1103 100 0.5675558992415226 1104 1 0.15247867625917522 1104 2 0.1638149611478923 1104 3 0.2931653662790765 1104 4 0.18472324575287047 1104 5 0.1558302549874071 1104 6 0.16889192830700495 1104 7 0.16226649525854953 1104 8 0.15482193691556034 1104 9 0.16466022690381266 1104 10-11 0.18375073413206877 1104 12-13 0.18145294529741562 1104 14-15 0.25778183081530415 1104 16-17 0.2489255254294207 1104 18-19 0.26925236252736795 1104 20-21 0.25922200828137676 1104 22-23 0.2462678484190448 1104 24-25 0.2753248283368066 1104 26-27 0.26870076707339763 1104 28-29 0.2851339714171459 1104 30-31 0.3094593670746093 1104 32-33 0.281825963600042 1104 34-35 0.28168113295106423 1104 36-37 0.3143374037139566 1104 38-39 0.32195302239043855 1104 40-41 0.3245185540918314 1104 42-43 0.39375029777198023 1104 44-45 0.34368506679403055 1104 46-47 0.39653492526209533 1104 48-49 0.4271390961924908 1104 50-51 0.3600984673781795 1104 52-53 0.35030404001422966 1104 54-55 0.3851382558906593 1104 56-57 0.3979363718568578 1104 58-59 0.4824922946575825 1104 60-61 0.4988353934133798 1104 62-63 0.4858503285199234 1104 64-65 0.434108992834517 1104 66-67 0.4942670476050566 1104 68-69 0.45088153395678887 1104 70-71 0.45946833791923325 1104 72-73 0.4401472755021203 1104 74-75 0.4550074694335464 1104 76-77 0.4433937501059191 1104 78-79 0.4058256191166194 1104 80-81 0.3947875303359538 1104 82-83 0.41664986389134384 1104 84-85 0.41635845362905854 1104 86-87 0.3793803201625181 1104 88-89 0.4209668394636239 1104 90-91 0.40969530414529487 1104 92-93 0.48942034661430966 1104 94-95 0.5221071707454357 1104 96-97 0.5529705605703654 1104 98-99 0.5510726430990083 1104 100 0.5436690686663788 1105 1 0.18537920267044328 1105 2 0.14894346137099745 1105 3 0.28709915894283 1105 4 0.14960030404552072 1105 5 0.1432959350722527 1105 6 0.12919011856786966 1105 7 0.12873935638960887 1105 8 0.12737643255672992 1105 9 0.17224723113538687 1105 10-11 0.19434668807691224 1105 12-13 0.1616534470843618 1105 14-15 0.2653685096414833 1105 16-17 0.2485925617566096 1105 18-19 0.17688738772024948 1105 20-21 0.2180001929736619 1105 22-23 0.1945362672221691 1105 24-25 0.2054505135074436 1105 26-27 0.17971338931193515 1105 28-29 0.18416104035350855 1105 30-31 0.2051864703382833 1105 32-33 0.2364057457251576 1105 34-35 0.20923693642608754 1105 36-37 0.21593869554970269 1105 38-39 0.24016162251181328 1105 40-41 0.2399272035091684 1105 42-43 0.23304427397384586 1105 44-45 0.20185189758334587 1105 46-47 0.27216851174898693 1105 48-49 0.23822452197124733 1105 50-51 0.24329109541957905 1105 52-53 0.18640686166947518 1105 54-55 0.21325775912796274 1105 56-57 0.187897877286332 1105 58-59 0.23206733740106245 1105 60-61 0.18442405827741482 1105 62-63 0.2561690775976899 1105 64-65 0.20802211811429316 1105 66-67 0.2300223599664264 1105 68-69 0.2412789051190316 1105 70-71 0.2541436067070464 1105 72-73 0.3072636272769458 1105 74-75 0.2895932215643242 1105 76-77 0.21016196396281828 1105 78-79 0.2580555912133988 1105 80-81 0.23985068531044362 1105 82-83 0.26646785725946387 1105 84-85 0.18717804598011867 1105 86-87 0.17641616102248037 1105 88-89 0.2259590862363936 1105 90-91 0.26202172935879986 1105 92-93 0.29402382268938254 1105 94-95 0.29014060514376894 1105 96-97 0.3174901639970429 1105 98-99 0.3148451083477859 1105 100 0.36066557215584893 1106 1 0.17443422040637913 1106 2 0.17368785873006942 1106 3 0.28694504147109257 1106 4 0.16133118457369733 1106 5 0.15230195734277885 1106 6 0.1422592972153467 1106 7 0.1755045285168535 1106 8 0.15694673355890387 1106 9 0.21989482556490003 1106 10-11 0.16486634929628252 1106 12-13 0.14340813138343123 1106 14-15 0.2097112167334103 1106 16-17 0.22559409051785195 1106 18-19 0.2496540350915808 1106 20-21 0.22688808124075877 1106 22-23 0.223737764304893 1106 24-25 0.19843578242801385 1106 26-27 0.24317293781464144 1106 28-29 0.24192181447288164 1106 30-31 0.26194690119795183 1106 32-33 0.19141804472833712 1106 34-35 0.23430517789844174 1106 36-37 0.23131064573238547 1106 38-39 0.24421006451215277 1106 40-41 0.23802393582108294 1106 42-43 0.25023725609110414 1106 44-45 0.239568360124494 1106 46-47 0.21012794188333572 1106 48-49 0.1664455061153447 1106 50-51 0.15474561552552046 1106 52-53 0.12453912891687224 1106 54-55 0.11512055801942012 1106 56-57 0.12730017561514728 1106 58-59 0.18793022963507156 1106 60-61 0.11067702295444803 1106 62-63 0.12493859722669498 1106 64-65 0.08468423904226796 1106 66-67 0.07892702375911398 1106 68-69 0.09815821422280635 1106 70-71 0.09558417570915623 1106 72-73 0.16321498614962593 1106 74-75 0.14812702454950255 1106 76-77 0.06656159291055275 1106 78-79 0.13280296837576344 1106 80-81 0.11243799624561746 1106 82-83 0.15349203110545062 1106 84-85 0.12012303386859458 1106 86-87 0.16300267164327664 1106 88-89 0.13330808932033733 1106 90-91 0.1237019163029629 1106 92-93 0.24222015542359543 1106 94-95 0.2202876386941952 1106 96-97 0.2626374392836546 1106 98-99 0.2678271713522413 1106 100 0.306380472984646 1107 1 0.13759514420221208 1107 2 0.091564876847265 1107 3 0.19222852753774333 1107 4 0.1691719124779567 1107 5 0.15462175882122153 1107 6 0.13380148798323432 1107 7 0.09280435195618963 1107 8 0.12295946293237847 1107 9 0.15864374344589294 1107 10-11 0.14733572405257434 1107 12-13 0.10919414719586484 1107 14-15 0.1752895737115665 1107 16-17 0.16078765431728215 1107 18-19 0.13987310864419555 1107 20-21 0.12129384235009866 1107 22-23 0.12526583030274452 1107 24-25 0.11919996679982603 1107 26-27 0.2086308949205602 1107 28-29 0.22485949777824743 1107 30-31 0.20682523474478387 1107 32-33 0.23947348479300956 1107 34-35 0.2114204130027204 1107 36-37 0.19712109280946066 1107 38-39 0.23856768710958676 1107 40-41 0.26737837343356574 1107 42-43 0.28853530684269657 1107 44-45 0.31444307399932114 1107 46-47 0.3562490467700954 1107 48-49 0.3749031867254544 1107 50-51 0.42533211275488014 1107 52-53 0.3260986457595365 1107 54-55 0.3444749257887736 1107 56-57 0.3235816921885686 1107 58-59 0.3512051180541036 1107 60-61 0.3770536737521155 1107 62-63 0.3742232583728722 1107 64-65 0.3157097834542313 1107 66-67 0.37039229096495774 1107 68-69 0.360041192711094 1107 70-71 0.3414982040182153 1107 72-73 0.4209107065803934 1107 74-75 0.4034905169570848 1107 76-77 0.3751183760793424 1107 78-79 0.4053633015670428 1107 80-81 0.45015094898156605 1107 82-83 0.46642556849504757 1107 84-85 0.4465871007407429 1107 86-87 0.37843644988794267 1107 88-89 0.4042326653248338 1107 90-91 0.42872275624777245 1107 92-93 0.4662480130676414 1107 94-95 0.4538333306358808 1107 96-97 0.4488318896897354 1107 98-99 0.48838856585480883 1107 100 0.5390839674396659 1108 1 0.06889798229457256 1108 2 0.07320484597978805 1108 3 0.14221944280281207 1108 4 0.08073350352112385 1108 5 0.06499861007000618 1108 6 0.05011580233715307 1108 7 0.03337706580285982 1108 8 -0.027376531159177375 1108 9 0.07061511763063066 1108 10-11 0.06064545498406204 1108 12-13 0.0383588401441699 1108 14-15 0.13611367245797368 1108 16-17 0.07376024199793108 1108 18-19 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-0.38376568649484 2215 12-13 -0.37812688206987133 2215 14-15 -0.41922473038581387 2215 16-17 -0.449212301903934 2215 18-19 -0.4472361090192365 2215 20-21 -0.44483602801388145 2215 22-23 -0.4299856018547885 2215 24-25 -0.4057783125209298 2215 26-27 -0.44967157301815064 2215 28-29 -0.43200537941557826 2215 30-31 -0.43489793334551763 2215 32-33 -0.38112953939960903 2215 34-35 -0.43914245776913674 2215 36-37 -0.44003629465671423 2215 38-39 -0.43934568885406833 2215 40-41 -0.4115988214145645 2215 42-43 -0.33443404454687453 2215 44-45 -0.4264420445813357 2215 46-47 -0.4908571305322056 2215 48-49 -0.4420892808578216 2215 50-51 -0.4864795560782582 2215 52-53 -0.4058547172766893 2215 54-55 -0.302480019573764 2215 56-57 -0.38479729553108655 2215 58-59 -0.39335895324050085 2215 60-61 -0.42591516302706367 2215 62-63 -0.3917558234303513 2215 64-65 -0.37337288287371706 2215 66-67 -0.3791823265718648 2215 68-69 -0.41259380477761454 2215 70-71 -0.47322965355153457 2215 72-73 -0.4858565056234241 2215 74-75 -0.4375920965008504 2215 76-77 -0.33948771608356765 2215 78-79 -0.41587253797387547 2215 80-81 -0.48351180172402763 2215 82-83 -0.4261049505598109 2215 84-85 -0.4426657962895959 2215 86-87 -0.3728680474798054 2215 88-89 -0.45079225830648895 2215 90-91 -0.43639723650966644 2215 92-93 -0.3996045838303388 2215 94-95 -0.48719821148669595 2215 96-97 -0.5286706986959828 2215 98-99 -0.45838929124724714 2215 100 -0.4626657917616406 2216 1 -0.344928204274769 2216 2 -0.3735412067469639 2216 3 -0.5179515508188466 2216 4 -0.2697784796966687 2216 5 -0.4679718824765615 2216 6 -0.3610325879118932 2216 7 -0.5797760518187047 2216 8 -0.35282091439906793 2216 9 -0.5811377588217042 2216 10-11 -0.42775117071696656 2216 12-13 -0.3979053017634442 2216 14-15 -0.6480357679732407 2216 16-17 -0.5084664275827464 2216 18-19 -0.4623271484015774 2216 20-21 -0.47633761993583335 2216 22-23 -0.5035083658174742 2216 24-25 -0.4651955359787934 2216 26-27 -0.5841077313802714 2216 28-29 -0.5866521023740177 2216 30-31 -0.6627089677601319 2216 32-33 -0.6534546716115486 2216 34-35 -0.6125538483850619 2216 36-37 -0.6813286938469574 2216 38-39 -0.6939945421308806 2216 40-41 -0.6349007574811409 2216 42-43 -0.6821889533820951 2216 44-45 -0.8720147897413142 2216 46-47 -0.8141012911557937 2216 48-49 -0.853948529060915 2216 50-51 -0.740495130669693 2216 52-53 -0.6563218764548324 2216 54-55 -0.6113715756413995 2216 56-57 -0.7699159489764824 2216 58-59 -0.7606772858085904 2216 60-61 -0.6271130818419905 2216 62-63 -0.5786217609842268 2216 64-65 -0.7071970959514786 2216 66-67 -0.6104811907495673 2216 68-69 -0.6437911766597324 2216 70-71 -0.7386417137824353 2216 72-73 -0.7299912556638048 2216 74-75 -0.7358014279298928 2216 76-77 -0.6567710268219287 2216 78-79 -0.8344543381956484 2216 80-81 -0.985737228992619 2216 82-83 -0.8729649897002574 2216 84-85 -0.997460606592302 2216 86-87 -0.828403241623576 2216 88-89 -1.1470270641128621 2216 90-91 -0.9142079309798561 2216 92-93 -1.0178144773008206 2216 94-95 -0.9926757737195651 2216 96-97 -1.0173443926937296 2216 98-99 -1.189553990693316 2216 100 -1.1558925426982611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 497.0 3 62.0 4 73.0 5 121.0 6 367.0 7 1062.0 8 2421.0 9 4170.0 10 6216.0 11 8852.0 12 12464.0 13 17558.0 14 21018.0 15 26637.0 16 32931.0 17 44395.0 18 44295.0 19 25398.0 20 19686.0 21 19781.0 22 21190.0 23 23020.0 24 27345.0 25 34078.0 26 43220.0 27 55732.0 28 73488.0 29 95931.0 30 124996.0 31 161110.0 32 208503.0 33 272318.0 34 370935.0 35 535636.0 36 860592.0 37 1574635.0 38 2298686.0 39 987889.0 40 4209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.147511912814785 20.760283229583663 14.856014372192583 23.236190485408965 2 15.214282870085121 20.619382183278905 40.40497588729268 23.7613590593433 3 17.553259516788216 25.61808155958736 31.263631894592542 25.565027029031878 4 12.389752340411702 17.591259899356313 38.63709635503725 31.381891405194732 5 12.396475750159679 37.89116862248135 35.998534767091606 13.713820860267367 6 30.13401820029655 38.72477103254884 17.409130316291584 13.732080450863032 7 25.930925903896252 33.29313825177073 22.78987937382009 17.98605647051293 8 24.444252584618557 36.75329261852813 20.473188354290507 18.329266442562812 9 24.37275763358187 16.799009417210183 22.0121597461123 36.81607320309565 10-11 23.46911877752041 27.26520951329632 29.054618380138624 20.211053329044645 12-13 24.091633730159913 24.843160673562732 29.38407367253716 21.681131923740196 14-15 21.359466576195583 26.901463528998764 27.04657906725262 24.692490827553033 16-17 20.37816207545056 30.222878646785713 27.808649414247967 21.590309863515763 18-19 20.194396559156218 28.562846344810545 30.765919689249323 20.476837406783915 20-21 21.320568811479635 27.768106996318682 30.513231328469313 20.39809286373237 22-23 20.95760636614672 27.629229089264463 30.63613833475759 20.777026209831227 24-25 20.869493863801626 27.756091727773942 30.546029488782867 20.828384919641564 26-27 20.64527121130354 27.74215548589256 30.634033241541175 20.97854006126272 28-29 20.67017536590954 27.73433734992068 30.768715498765925 20.826771785403857 30-31 21.007171603951416 28.0541339867092 30.325059207226033 20.61363520211335 32-33 20.67207885584987 27.844427605824208 30.688720311103605 20.794773227222322 34-35 20.884531887152118 27.576580904786162 30.628082317611334 20.910804890450382 36-37 21.14305150561762 27.864448206905184 30.124881086158933 20.867619201318263 38-39 20.96310489519338 28.029094375177195 30.189811696709402 20.817989032920035 40-41 20.88183906106862 27.78569882422699 30.320399230664748 21.012062884039644 42-43 20.849214424061902 27.82876307732308 30.180764708786505 21.14125778982851 44-45 20.997367237388005 27.963396114913685 29.967305067770315 21.071931579927988 46-47 21.140962033883504 28.142533949000086 29.53293206825922 21.183571948857185 48-49 21.09032101725105 28.100472973136416 29.40700567385414 21.402200335758394 50-51 21.150802476815976 28.06033483433995 29.27282946466771 21.516033224176358 52-53 21.14258913053375 28.185473281958874 29.21089509435198 21.4610424931554 54-55 21.197332400171234 28.48761995162426 29.01326171041319 21.301785937791315 56-57 21.09742067401756 28.225472375280603 29.048064641836568 21.62904230886527 58-59 20.95739206976763 27.90223139503497 29.37861222918205 21.761764306015355 60-61 21.17280450516821 28.219665652669722 29.091718324772682 21.515811517389388 62-63 21.003924052493623 28.263523504774863 29.068528915411047 21.664023527320474 64-65 20.97309765678665 28.03763208272434 29.210612577292792 21.778657683196215 66-67 20.969149180901237 28.084826060714963 29.038011494418985 21.908013263964822 68-69 21.249997829156342 28.196226155763366 28.908653627876706 21.645122387203582 70-71 21.290392647054173 28.040688328000464 28.900338542201293 21.768580482744074 72-73 21.04920362843453 28.17017881921315 28.96625267104951 21.814364881302815 74-75 21.180203463636555 28.17463578624689 28.83256410563348 21.81259664448308 76-77 21.248901019326862 28.2109633536657 28.630627440047213 21.90950818696022 78-79 21.267206322795445 28.27003324362634 28.700066100215665 21.762694333362546 80-81 21.21839774567154 28.133436923014326 28.69715549318893 21.951009838125206 82-83 21.071821803568515 28.22981900405339 28.78961038509955 21.908748807278542 84-85 21.163559488870316 28.063027451840473 28.66091009605049 22.112502963238718 86-87 21.24579127711655 28.180526107029408 28.61735564588332 21.95632696997072 88-89 21.078018514671854 28.07817909598489 28.64620525393029 22.19759713541297 90-91 21.011770533307107 27.935111253189554 28.681233966167508 22.371884247335828 92-93 21.055961000838387 28.133844144149677 28.543988833564903 22.266206021447033 94-95 20.983046600295367 27.92256502276642 28.674976414845656 22.419411962092564 96-97 21.00222118033862 27.92533717921944 28.66612758597313 22.40631405446882 98-99 20.98126927463756 27.962213414543 28.567124040637005 22.489393270182433 100 21.07077785077317 27.901880965470976 28.481867647671766 22.54547353608409 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1215.0 1 875.5 2 990.5 3 1458.5 4 962.5 5 583.0 6 1369.5 7 3834.0 8 6493.5 9 7375.0 10 5774.0 11 3754.5 12 3504.0 13 4021.0 14 4747.0 15 5450.0 16 6143.5 17 7103.5 18 8326.5 19 9757.0 20 11858.5 21 15018.0 22 19389.5 23 25998.0 24 35213.5 25 46987.0 26 63254.5 27 83251.0 28 106060.0 29 132062.5 30 157880.0 31 181066.5 32 200406.0 33 218152.0 34 235248.5 35 248993.0 36 261706.0 37 275258.0 38 286850.0 39 293424.5 40 297847.0 41 299757.0 42 297702.5 43 294439.5 44 288355.5 45 280084.5 46 272352.5 47 261658.0 48 249041.0 49 239717.5 50 232148.5 51 222542.5 52 208113.0 53 194093.0 54 183526.0 55 173817.5 56 160199.0 57 144090.0 58 131090.5 59 119114.5 60 104289.5 61 87161.0 62 72672.5 63 60881.5 64 49506.5 65 39163.0 66 30560.5 67 23280.5 68 17622.5 69 13478.0 70 9989.5 71 7093.0 72 4998.0 73 3368.0 74 2322.5 75 1558.0 76 966.0 77 636.5 78 426.5 79 267.0 80 163.5 81 80.5 82 50.0 83 37.5 84 19.5 85 10.5 86 4.5 87 1.5 88 2.0 89 1.5 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03942186067461 2 0.0 3 0.0 4 9.923690541122719E-5 5 0.0 6 0.0 7 0.0 8 9.427506014066583E-4 9 0.0 10-11 0.0 12-13 0.0445201566901118 14-15 1.1784382517583229E-4 16-17 0.0 18-19 7.442767905842039E-5 20-21 7.442767905842039E-5 22-23 0.0 24-25 1.364507449404374E-4 26-27 9.30345988230255E-5 28-29 3.72138395292102E-4 30-31 1.2404613176403399E-5 32-33 2.7910379646907646E-4 34-35 1.24046131764034E-4 36-37 9.923690541122719E-5 38-39 3.4112686235109345E-4 40-41 1.4885535811684077E-4 42-43 5.333983665853462E-4 44-45 2.8530610305727813E-4 46-47 1.6125997129324418E-4 48-49 8.125021630544226E-4 50-51 4.7137530070332916E-4 52-53 6.698491115257835E-4 54-55 4.7137530070332916E-4 56-57 6.264329654083716E-4 58-59 1.6125997129324418E-4 60-61 2.48092263528068E-4 62-63 9.985713607004736E-4 64-65 5.520052863499513E-4 66-67 0.0015691835668150298 68-69 0.0016746227788144586 70-71 5.023868336443377E-4 72-73 9.799644409358686E-4 74-75 9.489529079948599E-4 76-77 8.621206157600362E-4 78-79 0.003380257090569926 80-81 0.0027910379646907645 82-83 0.0015009581943448112 84-85 0.002927488709631202 86-87 0.0016684204722262571 88-89 0.0022390326783408134 90-91 0.003008118695277824 92-93 0.0026731941395149324 94-95 0.0037461931792738263 96-97 0.003256210958805892 98-99 0.0030205233084542277 100 0.0015505766470504246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8061517.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.00766928536282 #Duplication Level Percentage of deduplicated Percentage of total 1 72.42420249831248 40.56310781781634 2 15.92289162446224 17.836080963391097 3 5.254675570316334 8.829063946324576 4 2.2656559660687714 5.0757644024795585 5 1.20698542582655 3.380022028097312 6 0.6944740881785227 2.3337525034773963 7 0.4488772144937211 1.7598396605369444 8 0.30834257071609916 1.3815638981812706 9 0.22976263431938368 1.1581622673384415 >10 1.1515130819012207 11.387856103711556 >50 0.06472654617523046 2.467307461436242 >100 0.026694392997219936 2.5773835218226107 >500 7.768306122025164E-4 0.28261795589064004 >1k 3.3280707147439505E-4 0.3069096669389633 >5k 2.218713717251777E-5 0.06630779552362287 >10k+ 6.656141151755331E-5 0.5942600070334882 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18719 0.23220195404909522 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18310 0.22712846725994623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10793 0.1338829900129219 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.2404613176403399E-5 0.0 0.0 0.0 3 0.0 1.2404613176403399E-5 0.0 1.2404613176403399E-5 0.0 4 0.0 1.2404613176403399E-5 0.0 1.2404613176403399E-5 0.0 5 0.0 1.2404613176403399E-5 0.0 1.2404613176403399E-5 0.0 6 0.0 2.4809226352806797E-5 0.0 1.2404613176403399E-5 0.0 7 0.0 2.4809226352806797E-5 0.0 1.2404613176403399E-5 0.0 8 0.0 2.4809226352806797E-5 0.0 1.2404613176403399E-5 0.0 9 0.0 2.4809226352806797E-5 0.0 1.2404613176403399E-5 0.0 10-11 0.0 2.4809226352806797E-5 0.0 1.2404613176403399E-5 0.0 12-13 0.0 2.4809226352806797E-5 0.0 1.2404613176403399E-5 0.0 14-15 0.0 4.341614611741189E-5 0.0 3.721383952921019E-5 0.0 16-17 0.0 6.202306588201699E-5 0.0 8.06299856466221E-5 0.0 18-19 0.0 7.44276790584204E-5 0.0 1.3024843835223569E-4 0.0 20-21 0.0 8.68322922348238E-5 0.0 1.8606919764605097E-4 0.0 22-23 0.0 9.923690541122719E-5 0.0 3.6593608870390026E-4 0.0 24-25 0.0 9.923690541122719E-5 0.0 8.497160025836329E-4 0.0 26-27 0.0 9.923690541122719E-5 0.0 0.0016994320051672656 0.0 28-29 0.0 9.923690541122719E-5 0.0 0.005346388279029865 0.0 30-31 0.0 9.923690541122719E-5 0.0 0.016287257100617663 0.0 32-33 0.0 9.923690541122719E-5 0.0 0.041760130258362047 0.0 34-35 0.0 9.923690541122719E-5 0.0 0.08195107694990905 0.0 36-37 0.0 9.923690541122719E-5 0.0 0.14278329996699132 0.0 38-39 0.0 9.923690541122719E-5 0.0 0.22433122698866725 0.0 40-41 0.0 1.2404613176403398E-4 0.0 0.30843450432468233 0.0 42-43 0.0 1.2404613176403398E-4 0.0 0.3927176485517552 0.0 44-45 0.0 1.4265305152863907E-4 0.0 0.4971644914970718 0.0 46-47 0.0 1.6125997129324418E-4 0.0 0.6205841902956974 0.0 48-49 0.0 1.6125997129324418E-4 0.0 0.7502682187484069 0.0 50-51 0.0 1.736645844696476E-4 0.0 0.8770185561848967 0.0 52-53 0.0 2.0467611741065608E-4 0.0 1.0017965601263386 0.0 54-55 0.0 2.108784239988578E-4 0.0 1.1403871008397055 0.0 56-57 0.0 2.1708073058705947E-4 0.0 1.3025091927487096 0.0 58-59 0.0 2.2328303717526117E-4 0.0 1.4778732092235245 0.0 60-61 0.0 2.2328303717526117E-4 0.0 1.6545459123884498 0.0 62-63 0.0 2.2328303717526117E-4 0.0 1.8287625021444476 0.0 64-65 0.0 2.3568765035166458E-4 0.0 2.0153216820109665 0.0 66-67 0.0 2.3568765035166458E-4 0.0 2.2262621290757063 0.0 68-69 0.0 2.3568765035166458E-4 0.0 2.4527021403043623 0.0 70-71 0.0 2.3568765035166458E-4 0.0 2.69089552251766 0.0 72-73 0.0 2.3568765035166458E-4 0.0 2.934683385273516 0.0 74-75 0.0 2.3568765035166458E-4 0.0 3.182031372011 0.0 76-77 0.0 2.4809226352806796E-4 0.0 3.4420382664950036 0.0 78-79 0.0 2.6049687670447137E-4 0.0 3.7319030648946097 0.0 80-81 0.0 2.6049687670447137E-4 0.0 4.038334224191303 0.0 82-83 0.0 2.6049687670447137E-4 0.0 4.349659003386087 0.0 84-85 0.0 2.6049687670447137E-4 0.0 4.65829818382818 0.0 86-87 0.0 2.729014898808748E-4 0.0 4.9822818707694845 0.0 88 0.0 2.853061030572782E-4 0.0 5.244806901728298 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12775 0.0 58.8822 1 GTATCAA 24150 0.0 41.640755 1 TCAACGC 29515 0.0 33.565346 4 ATCAACG 29600 0.0 33.453083 3 CAACGCA 30510 0.0 32.516914 5 TATCAAC 31130 0.0 32.005165 2 AACGCAG 31810 0.0 31.24257 6 ACGCAGA 36490 0.0 27.13246 7 CGCAGAG 36615 0.0 26.87284 8 GTGGTAT 5830 0.0 26.127714 1 GCAGAGT 39705 0.0 24.425962 9 TGGTATC 5860 0.0 23.658527 2 GTACATG 21400 0.0 23.089468 1 TACATGG 21560 0.0 21.64531 2 CAGAGTA 39115 0.0 21.19909 10-11 GAGTACT 28600 0.0 21.181307 12-13 AGAGTAC 37065 0.0 20.95035 10-11 ACATGGG 22210 0.0 19.932678 3 GTACTTT 31270 0.0 19.25978 14-15 AGTACTT 30210 0.0 19.056469 12-13 >>END_MODULE