FastQCFastQC Report
Wed 25 May 2016
SRR2049384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6357532
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.045.32461
GTATCAA106700.033.086731
TCAACGC111950.030.5258774
ATCAACG112600.030.3496653
CAACGCA118400.028.8236925
AACGCAG127950.026.8900176
TATCAAC129700.026.5657372
GTACATG169600.025.8049031
TACATGG170500.024.5371152
ACGCAGA146000.023.2436667
CGCAGAG147600.022.8006368
ACATGGG177400.022.7348213
GTGGTAT40700.020.5590931
TGGTATC38800.019.384122
GCAGAGT167150.019.0934189
GAGTACA127950.018.6271341
CATGGGG112650.018.3185544
CATGGGT51100.017.4779534
AGAGTAC143000.017.23970210-11
CAGAGTA161350.016.98321310-11
Produced by FastQC (version 0.11.5)