##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049384_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6357532 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.78558613625539 31.0 31.0 34.0 28.0 34.0 2 30.98802994621183 31.0 31.0 34.0 28.0 34.0 3 31.039306919729228 31.0 31.0 34.0 28.0 34.0 4 34.64299306712101 37.0 35.0 37.0 32.0 37.0 5 34.673616428513455 37.0 35.0 37.0 32.0 37.0 6 34.6512473708351 37.0 35.0 37.0 32.0 37.0 7 34.60394458101037 37.0 35.0 37.0 32.0 37.0 8 34.57001978126103 37.0 35.0 37.0 32.0 37.0 9 36.14827719309946 39.0 37.0 39.0 32.0 39.0 10-11 36.110804161111574 39.0 37.0 39.0 32.0 39.0 12-13 36.05850399180059 39.0 37.0 39.0 32.0 39.0 14-15 37.264490921948955 40.0 38.0 41.0 32.0 41.0 16-17 37.234405741095756 40.0 38.0 41.0 32.0 41.0 18-19 37.26707447166605 40.0 38.0 41.0 32.0 41.0 20-21 37.34972486178599 40.0 38.0 41.0 32.0 41.0 22-23 37.29340450036272 40.0 38.0 41.0 32.0 41.0 24-25 37.2977705814143 40.0 38.0 41.0 32.0 41.0 26-27 37.28557331681539 40.0 38.0 41.0 32.0 41.0 28-29 37.219166494167865 40.0 37.5 41.0 31.0 41.0 30-31 37.14430363858176 40.0 37.0 41.0 31.0 41.0 32-33 37.07912048260237 40.0 37.0 41.0 31.0 41.0 34-35 36.96501889412433 40.0 37.0 41.0 30.5 41.0 36-37 36.878493336722485 40.0 37.0 41.0 30.0 41.0 38-39 36.77596605097701 40.0 37.0 41.0 30.0 41.0 40-41 36.67178670905628 40.0 37.0 41.0 30.0 41.0 42-43 36.532580488780866 40.0 37.0 41.0 30.0 41.0 44-45 36.41535882163079 40.0 36.0 41.0 30.0 41.0 46-47 36.30280563275183 40.0 36.0 41.0 30.0 41.0 48-49 36.1801036156798 40.0 36.0 41.0 29.0 41.0 50-51 36.047177584005865 39.5 36.0 41.0 29.0 41.0 52-53 35.911795646486716 39.0 35.0 41.0 28.5 41.0 54-55 35.77770147283569 39.0 35.0 41.0 28.0 41.0 56-57 35.657766331337385 39.0 35.0 41.0 28.0 41.0 58-59 35.456853618668376 39.0 35.0 41.0 28.0 41.0 60-61 35.23922176089715 39.0 35.0 40.0 27.5 41.0 62-63 35.02495024798931 38.0 34.5 40.0 27.0 41.0 64-65 34.764287305199566 38.0 34.0 40.0 26.0 41.0 66-67 34.47467940389447 37.5 34.0 40.0 26.0 41.0 68-69 34.14742120055392 37.0 34.0 40.0 26.0 41.0 70-71 33.81060748101622 36.5 34.0 39.5 25.5 41.0 72-73 33.4139265048135 36.0 33.0 39.0 24.5 41.0 74-75 33.00562199293688 35.5 33.0 39.0 23.0 40.5 76-77 32.59427408308759 35.0 33.0 38.0 22.0 40.0 78-79 32.1681009234401 35.0 32.5 37.0 20.5 39.0 80-81 31.67974663753167 35.0 32.0 37.0 17.5 39.0 82-83 31.23056132474048 35.0 32.0 36.0 10.5 39.0 84-85 30.814910880511494 35.0 32.0 36.0 4.5 37.0 86-87 30.368910923295395 35.0 31.0 35.5 2.0 37.0 88-89 29.964208280823442 35.0 31.0 35.0 2.0 37.0 90-91 29.592177121562266 35.0 31.0 35.0 2.0 36.0 92-93 29.198847367185884 34.5 31.0 35.0 2.0 36.0 94-95 28.85862855271511 34.0 30.0 35.0 2.0 36.0 96-97 28.472986372699342 34.0 30.0 35.0 2.0 35.5 98-99 28.121224792891333 34.0 29.0 35.0 2.0 35.0 100 27.897871532538097 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45813847039788413 1101 2 0.4492955705192365 1101 3 0.39207356392229187 1101 4 0.42607315740500695 1101 5 0.4582142481774696 1101 6 0.5331002873434443 1101 7 0.5516348675786062 1101 8 0.517557656435514 1101 9 0.6903733965429808 1101 10-11 0.6651328382024104 1101 12-13 0.7033616952765911 1101 14-15 0.8020272813992122 1101 16-17 0.757543712819583 1101 18-19 0.7414651104054144 1101 20-21 0.7417374875249934 1101 22-23 0.713763069316073 1101 24-25 0.7418762987234544 1101 26-27 0.6423334594253873 1101 28-29 0.7017154589048502 1101 30-31 0.7258601757224099 1101 32-33 0.6966615543902819 1101 34-35 0.7627956411679762 1101 36-37 0.7463403771558816 1101 38-39 0.7933484392682288 1101 40-41 0.8220009101835473 1101 42-43 0.7614195499123184 1101 44-45 0.7585213758832907 1101 46-47 0.7072329690959407 1101 48-49 0.6813208681204515 1101 50-51 0.7326414477366683 1101 52-53 0.6594582551834733 1101 54-55 0.6699089326483758 1101 56-57 0.6827960608311443 1101 58-59 0.7931434887551063 1101 60-61 0.7788000515718423 1101 62-63 0.7891925676763947 1101 64-65 0.7652507157042976 1101 66-67 0.7474650035408459 1101 68-69 0.7114919934513324 1101 70-71 0.7517251420487341 1101 72-73 0.823588651186796 1101 74-75 0.7196837156102163 1101 76-77 0.7683558363988965 1101 78-79 0.7253071351564984 1101 80-81 0.7511704987116445 1101 82-83 0.760261363570482 1101 84-85 0.7033090536102051 1101 86-87 0.6791043886405106 1101 88-89 0.6958817011477159 1101 90-91 0.7118161976384343 1101 92-93 0.7728135461642438 1101 94-95 0.7814003386259714 1101 96-97 0.8272892129695251 1101 98-99 0.8364996063762966 1101 100 0.8744704705794852 1102 1 0.2721250369982897 1102 2 0.32745987819490807 1102 3 0.34856072572813446 1102 4 0.33925981251842785 1102 5 0.3319859265833074 1102 6 0.4009514893959363 1102 7 0.4053667203750493 1102 8 0.4143822911946202 1102 9 0.5151200175216744 1102 10-11 0.4325214402872959 1102 12-13 0.46836836000255033 1102 14-15 0.5913935785703899 1102 16-17 0.5221632512026488 1102 18-19 0.5039588335145311 1102 20-21 0.46918090757151276 1102 22-23 0.4801992705115268 1102 24-25 0.5462644065318969 1102 26-27 0.4714369120369284 1102 28-29 0.5196764360024986 1102 30-31 0.49159077300942755 1102 32-33 0.5500087023521871 1102 34-35 0.5910155385380094 1102 36-37 0.5633580225362778 1102 38-39 0.5970579275647978 1102 40-41 0.6053462416860711 1102 42-43 0.5290970924107725 1102 44-45 0.48300027253645794 1102 46-47 0.3837819994557492 1102 48-49 0.40172825769770526 1102 50-51 0.5235659166257278 1102 52-53 0.5054816594225926 1102 54-55 0.47327114121099356 1102 56-57 0.41008133472671204 1102 58-59 0.5222383053097701 1102 60-61 0.4390501782908842 1102 62-63 0.463090597631691 1102 64-65 0.4547851534904339 1102 66-67 0.5306000969323179 1102 68-69 0.5113163389419029 1102 70-71 0.5187999389635891 1102 72-73 0.45207709973887233 1102 74-75 0.4731361696547083 1102 76-77 0.5068610281390491 1102 78-79 0.46299642295480226 1102 80-81 0.3566257173443148 1102 82-83 0.40646727341582434 1102 84-85 0.3832254636669852 1102 86-87 0.43121156026888485 1102 88-89 0.46839110434368436 1102 90-91 0.48236207255630603 1102 92-93 0.39785459368381737 1102 94-95 0.35046827971913785 1102 96-97 0.3456482164788106 1102 98-99 0.44369814696143095 1102 100 0.4336452572833167 1103 1 0.2244222595995211 1103 2 0.2496994437002975 1103 3 0.2526221580042822 1103 4 0.2867527276279418 1103 5 0.2807594832886622 1103 6 0.37202406309682345 1103 7 0.36508115408273056 1103 8 0.3444554633328494 1103 9 0.4820466012739075 1103 10-11 0.40192816787167374 1103 12-13 0.4326222228061951 1103 14-15 0.5049664195194836 1103 16-17 0.5078632185445002 1103 18-19 0.5115986184079802 1103 20-21 0.49338880531771423 1103 22-23 0.4720881758179871 1103 24-25 0.4900638841447815 1103 26-27 0.5041855948392495 1103 28-29 0.4775709354994433 1103 30-31 0.4667397823505226 1103 32-33 0.5504546608169036 1103 34-35 0.5524447295302011 1103 36-37 0.5210439533975233 1103 38-39 0.5075647907112497 1103 40-41 0.48338438047890975 1103 42-43 0.4988044655861117 1103 44-45 0.4224404205103127 1103 46-47 0.44527560157198565 1103 48-49 0.48311090331126394 1103 50-51 0.43436880517184306 1103 52-53 0.4547971739960488 1103 54-55 0.4474773863744801 1103 56-57 0.40423611385070757 1103 58-59 0.47531278237860874 1103 60-61 0.4936244687731346 1103 62-63 0.4820821359681986 1103 64-65 0.44501562384438387 1103 66-67 0.5305419579536732 1103 68-69 0.5398490563268368 1103 70-71 0.49268747893123077 1103 72-73 0.4550869563606881 1103 74-75 0.45082147543864437 1103 76-77 0.49675734865767396 1103 78-79 0.46334860096515484 1103 80-81 0.4091850009675042 1103 82-83 0.4244254792533546 1103 84-85 0.5074291234091461 1103 86-87 0.4828003522807087 1103 88-89 0.5166851405089865 1103 90-91 0.5556890280560438 1103 92-93 0.48641294326147033 1103 94-95 0.5365752111111561 1103 96-97 0.48437810967343964 1103 98-99 0.5251177603913426 1103 100 0.4801333581032097 1104 1 0.1157768353994264 1104 2 0.13212522210144684 1104 3 0.13177853389885996 1104 4 0.18631359482573373 1104 5 0.1984727181547825 1104 6 0.2887377734640779 1104 7 0.28580486254428905 1104 8 0.2480655726128731 1104 9 0.334365560604752 1104 10-11 0.3481600255185242 1104 12-13 0.27891632012491385 1104 14-15 0.4226558362300281 1104 16-17 0.476177475444743 1104 18-19 0.3922853242664317 1104 20-21 0.37770793852887863 1104 22-23 0.39604624494577934 1104 24-25 0.42434861122511336 1104 26-27 0.3526434749182883 1104 28-29 0.3927483239516363 1104 30-31 0.336495972072008 1104 32-33 0.38102258597050565 1104 34-35 0.41321089740075223 1104 36-37 0.36739746142095697 1104 38-39 0.4409479895291639 1104 40-41 0.3776892769098552 1104 42-43 0.41935088771604256 1104 44-45 0.36787839550712675 1104 46-47 0.30795630858343515 1104 48-49 0.3282870916971561 1104 50-51 0.36940842831079834 1104 52-53 0.3792446616253997 1104 54-55 0.36565608390937854 1104 56-57 0.3461896584986661 1104 58-59 0.4545375323621954 1104 60-61 0.45210940565436886 1104 62-63 0.4301200969961627 1104 64-65 0.5036328649230768 1104 66-67 0.5503794586321789 1104 68-69 0.5338393028909039 1104 70-71 0.5765034845833199 1104 72-73 0.5398480980317544 1104 74-75 0.576564983198459 1104 76-77 0.5365661064073137 1104 78-79 0.4981201689904182 1104 80-81 0.597661849133658 1104 82-83 0.5219965984461581 1104 84-85 0.5072474074632609 1104 86-87 0.5097194652976214 1104 88-89 0.48549607090061286 1104 90-91 0.5197163521674995 1104 92-93 0.4834035112239796 1104 94-95 0.5090604406821413 1104 96-97 0.4915496431812336 1104 98-99 0.4735542729689719 1104 100 0.49530994683045293 1105 1 0.2234746367264826 1105 2 0.2576693412921074 1105 3 0.3054506139330435 1105 4 0.2520446970784249 1105 5 0.2805696274548737 1105 6 0.33100638497243295 1105 7 0.3117193825375608 1105 8 0.36744542199228647 1105 9 0.4547548455789041 1105 10-11 0.3687733463004079 1105 12-13 0.3364567612717053 1105 14-15 0.3698380607278082 1105 16-17 0.42396867014564776 1105 18-19 0.3916597702523674 1105 20-21 0.43194899288879185 1105 22-23 0.44613141211613083 1105 24-25 0.41967486584384517 1105 26-27 0.3933465588421896 1105 28-29 0.4657190710590484 1105 30-31 0.4746309242385962 1105 32-33 0.4950078285160586 1105 34-35 0.5270211589721754 1105 36-37 0.44825121817262215 1105 38-39 0.40447373781525897 1105 40-41 0.4321648002339771 1105 42-43 0.4371484832138606 1105 44-45 0.41174427735978725 1105 46-47 0.4002864583000232 1105 48-49 0.3865197387377748 1105 50-51 0.46375226430221517 1105 52-53 0.39470999544445107 1105 54-55 0.47023688079038806 1105 56-57 0.4416615894289535 1105 58-59 0.4643432745878755 1105 60-61 0.46716924748518096 1105 62-63 0.3606754480576342 1105 64-65 0.3652093386143491 1105 66-67 0.3570827537523016 1105 68-69 0.2784301703801546 1105 70-71 0.3656677966654698 1105 72-73 0.3102295620964952 1105 74-75 0.3737677732049818 1105 76-77 0.4257212202134113 1105 78-79 0.3308486088092337 1105 80-81 0.44642505305310465 1105 82-83 0.39867419538783366 1105 84-85 0.3760246193597183 1105 86-87 0.44238658063751757 1105 88-89 0.3861901528627918 1105 90-91 0.44108862571377827 1105 92-93 0.47076078952217415 1105 94-95 0.45362646016063835 1105 96-97 0.3849995682255738 1105 98-99 0.3649943509500879 1105 100 0.30612238574938644 1106 1 0.11906839079234643 1106 2 0.15888155248275027 1106 3 0.13731078919952466 1106 4 0.1587255632153699 1106 5 0.22204223713519156 1106 6 0.289738489348629 1106 7 0.2632266610638183 1106 8 0.24246147882833213 1106 9 0.3237644059919944 1106 10-11 0.2815805071033637 1106 12-13 0.3290252029780092 1106 14-15 0.3343767313527195 1106 16-17 0.3773207230781992 1106 18-19 0.38320607172956755 1106 20-21 0.31666070890031506 1106 22-23 0.3583305609483105 1106 24-25 0.37610761824849703 1106 26-27 0.30384760890380846 1106 28-29 0.31718976522896725 1106 30-31 0.2942003391642416 1106 32-33 0.38638018981729516 1106 34-35 0.4032806445766184 1106 36-37 0.31114336330110604 1106 38-39 0.3471252552966462 1106 40-41 0.3996856380160665 1106 42-43 0.3602075341777464 1106 44-45 0.30290372381882236 1106 46-47 0.3066222737421711 1106 48-49 0.33319333868063694 1106 50-51 0.4188170645377767 1106 52-53 0.4026320729512989 1106 54-55 0.39369246981124917 1106 56-57 0.3332421893519282 1106 58-59 0.3790662937854705 1106 60-61 0.39921174749964905 1106 62-63 0.3449736567693975 1106 64-65 0.37551453225097475 1106 66-67 0.39528885597203356 1106 68-69 0.46904057744580285 1106 70-71 0.4007394718150934 1106 72-73 0.4225865627312828 1106 74-75 0.41076213076863155 1106 76-77 0.4360784775835853 1106 78-79 0.3058694027653459 1106 80-81 0.3372259475901558 1106 82-83 0.3377341689373914 1106 84-85 0.31435029579893126 1106 86-87 0.3242423113195798 1106 88-89 0.3329296712016472 1106 90-91 0.3732734242041289 1106 92-93 0.3471409759889639 1106 94-95 0.3983149773337402 1106 96-97 0.3403224686428281 1106 98-99 0.38280216511891396 1106 100 0.411478633055939 1107 1 0.19311951510797343 1107 2 0.15713339766862333 1107 3 0.18232442461133402 1107 4 0.25400970262742106 1107 5 0.18322316417979323 1107 6 0.23357924998533264 1107 7 0.2685092209153126 1107 8 0.3341351074904111 1107 9 0.29373890451449824 1107 10-11 0.2809158001924814 1107 12-13 0.27593873442051375 1107 14-15 0.24918557218548898 1107 16-17 0.28845894730746124 1107 18-19 0.2658795786054853 1107 20-21 0.1952001245318442 1107 22-23 0.268336805786916 1107 24-25 0.29791136023256115 1107 26-27 0.3097512005189742 1107 28-29 0.4001141996818234 1107 30-31 0.3587353345368385 1107 32-33 0.3591150416847668 1107 34-35 0.355429980963315 1107 36-37 0.3084833707742334 1107 38-39 0.37747438158307034 1107 40-41 0.3984068035360053 1107 42-43 0.32635360365705424 1107 44-45 0.30716709052022395 1107 46-47 0.3031287194088037 1107 48-49 0.372128314870416 1107 50-51 0.4258683056183301 1107 52-53 0.4459216969533415 1107 54-55 0.46910862465618663 1107 56-57 0.3891018781623288 1107 58-59 0.44119526779834217 1107 60-61 0.45236175805061407 1107 62-63 0.3840105895079091 1107 64-65 0.4191588073843846 1107 66-67 0.3654131723238052 1107 68-69 0.42810023062246927 1107 70-71 0.36609084084771126 1107 72-73 0.4168496185843651 1107 74-75 0.48356528203449045 1107 76-77 0.6096175287502419 1107 78-79 0.4052340275109927 1107 80-81 0.3327416519837385 1107 82-83 0.35339156293396456 1107 84-85 0.38197267886985387 1107 86-87 0.4095863840334957 1107 88-89 0.3552495011929331 1107 90-91 0.33558901146682985 1107 92-93 0.34516240831081646 1107 94-95 0.2420181359910245 1107 96-97 0.18845630635753352 1107 98-99 0.15885472818782276 1107 100 0.1617178118001945 1108 1 -0.014466665085226538 1108 2 0.016454617395702087 1108 3 0.05906496815092055 1108 4 0.027594162711395143 1108 5 5.379297028582641E-4 1108 6 0.12484756995986146 1108 7 0.07226007659900802 1108 8 0.05008828828582068 1108 9 0.09956986766287912 1108 10-11 0.07613517449384233 1108 12-13 0.11715850992416676 1108 14-15 0.20052906109241775 1108 16-17 0.19587829040652593 1108 18-19 0.13389653513581123 1108 20-21 0.1254270516162066 1108 22-23 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-0.45938880144245076 2215 54-55 -0.5155098843606609 2215 56-57 -0.49188440921341225 2215 58-59 -0.4253013316395595 2215 60-61 -0.2897780432466561 2215 62-63 -0.34449819572166973 2215 64-65 -0.3154195005093783 2215 66-67 -0.2979080005150081 2215 68-69 -0.33133925912023443 2215 70-71 -0.37821299049514323 2215 72-73 -0.3507341703785727 2215 74-75 -0.37801588088466787 2215 76-77 -0.3892772809745395 2215 78-79 -0.4496179902862032 2215 80-81 -0.4609755918567231 2215 82-83 -0.3542210752828261 2215 84-85 -0.3558265472396549 2215 86-87 -0.2933530278201175 2215 88-89 -0.22669897387361715 2215 90-91 -0.2941447490239213 2215 92-93 -0.268993789815859 2215 94-95 -0.35258131123110203 2215 96-97 -0.36914094827859145 2215 98-99 -0.5076146335393013 2215 100 -0.46174431112878267 2216 1 -1.2961582326417478 2216 2 -1.2827135273304968 2216 3 -1.2859897297204874 2216 4 -1.4679513544734561 2216 5 -1.4539590703259577 2216 6 -2.8467618413152067 2216 7 -2.7512500524908923 2216 8 -3.6265447975169884 2216 9 -3.1311723234482542 2216 10-11 -2.22274302229723 2216 12-13 -1.974570011303932 2216 14-15 -2.187885653157089 2216 16-17 -2.553495085958019 2216 18-19 -2.2164484026023032 2216 20-21 -2.05813097857925 2216 22-23 -2.1952589650039585 2216 24-25 -2.3376987840567693 2216 26-27 -2.379968979215164 2216 28-29 -2.883161971069569 2216 30-31 -2.435982925712871 2216 32-33 -2.6213485408628046 2216 34-35 -3.5839683804303846 2216 36-37 -2.614008958581998 2216 38-39 -2.5801188239973385 2216 40-41 -3.0952750621783878 2216 42-43 -2.9207267706475903 2216 44-45 -2.636154265995742 2216 46-47 -2.499188958040449 2216 48-49 -2.681762182189935 2216 50-51 -2.9714050006354853 2216 52-53 -3.338607282085178 2216 54-55 -2.690625156641552 2216 56-57 -2.672780412784455 2216 58-59 -3.71334266350096 2216 60-61 -4.497358977905787 2216 62-63 -3.568547835240537 2216 64-65 -3.4896514473211724 2216 66-67 -3.0904832165514087 2216 68-69 -3.4805625404805838 2216 70-71 -3.2119645910767574 2216 72-73 -3.700890370379657 2216 74-75 -4.146574171638548 2216 76-77 -4.65825415124521 2216 78-79 -3.678338065717128 2216 80-81 -3.4685618186455116 2216 82-83 -3.6636316569454017 2216 84-85 -3.646089792889633 2216 86-87 -3.2571817406476313 2216 88-89 -3.2175022714949364 2216 90-91 -3.3330659960534668 2216 92-93 -3.4155429925696694 2216 94-95 -3.184800873144667 2216 96-97 -3.4148698462813343 2216 98-99 -3.1460912923713167 2216 100 -2.807991180431422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 122936.0 3 15256.0 4 9867.0 5 8281.0 6 7871.0 7 9113.0 8 10583.0 9 12067.0 10 13359.0 11 15316.0 12 17086.0 13 19088.0 14 20938.0 15 21347.0 16 21111.0 17 22083.0 18 21365.0 19 20154.0 20 20778.0 21 22158.0 22 25347.0 23 30020.0 24 36236.0 25 45010.0 26 55177.0 27 67699.0 28 84479.0 29 107490.0 30 131261.0 31 154876.0 32 188910.0 33 242231.0 34 318485.0 35 441767.0 36 695900.0 37 1183891.0 38 1546474.0 39 569450.0 40 2072.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.717628206691295 20.846708558093482 14.207473785870445 23.228189449344782 2 15.462634731462282 20.458113327606902 40.67246264432971 23.4067892966011 3 18.242440969240214 25.497581053510753 30.605676003729833 25.6543019735192 4 12.63043307565492 16.868806823644523 39.24572770849898 31.255032392201578 5 12.900032431712832 37.9429481953349 36.04129480741684 13.115724565535428 6 30.558479330176652 38.33364621087464 17.77792219624613 13.32995226270258 7 26.53140575519758 32.86728427843669 22.92840817450483 17.6729017918609 8 24.572787289926215 36.61614174839044 20.86151258790511 17.949558373778235 9 24.572829553685185 17.057990761168966 22.13130708672816 36.23787259841769 10-11 23.530447058927198 27.29036446716211 29.299274458219703 19.87991401569099 12-13 23.837007027642755 24.926054834842294 29.285322358748882 21.951615778766072 14-15 21.349230258076542 27.221594078943383 26.509455049011827 24.919720613968245 16-17 20.75960381426522 30.55175145674453 27.283081465583535 21.40556326340671 18-19 20.670025240046368 29.54072021737863 29.2074959027508 20.581758639824198 20-21 21.890535951632824 28.756066470722725 28.694888405891284 20.65850917175317 22-23 21.50240922867862 28.663755325212044 28.687625744554097 21.146209701555243 24-25 21.384013855432777 28.732258426416458 28.654221701085746 21.229506017065017 26-27 21.06223767624651 28.733048601581174 28.772692438018844 21.43202128415347 28-29 21.078282392195167 28.736796929488296 28.90136842639422 21.283552251922316 30-31 21.392237277382517 29.125913254079748 28.422885417138716 21.058964051399023 32-33 21.046462264299386 28.78735381511014 28.857885408543538 21.30829851204694 34-35 21.264317039557643 28.473504994752037 28.912255487351107 21.349922478339213 36-37 21.3925574685877 28.715711437325194 28.47689361230763 21.41483748177948 38-39 21.22671308564134 28.678092980714027 28.75951132941554 21.3356826042291 40-41 21.083114686387507 28.34532187321755 29.078905650728714 21.49265778966623 42-43 20.87875413979706 28.32463781889497 29.106282169574648 21.69032587173332 44-45 20.867308132269553 28.405134745854188 29.095062722584693 21.63249439929157 46-47 20.844853199347686 28.600387711034518 28.9352076030447 21.619551486573098 48-49 20.751460407273726 28.501262173600857 28.909624719799975 21.837652699325442 50-51 20.711337164705743 28.38223250215367 28.86044599692389 22.045984336216698 52-53 20.60848693556593 28.31477969888143 29.02426258222629 22.052470783326346 54-55 20.76290754594994 28.57016081790602 28.812708590691233 21.85422304545281 56-57 20.761577728291666 28.330159987903475 28.693622244393595 22.214640039411265 58-59 20.73925984015085 27.930921791735404 28.853674900385368 22.47614346772838 60-61 20.885731984606146 28.11799280720092 28.489708872110132 22.506566336082805 62-63 20.858306728480713 28.005290209831195 28.520881908283258 22.615521153404835 64-65 20.89414202909975 27.762310011791797 28.48592139595218 22.857626563156277 66-67 20.740919934939395 27.85872329378168 28.25969172916064 23.140665042118286 68-69 21.04867234358577 27.86446827659561 28.207789714832366 22.879069664986247 70-71 20.916026931047014 27.598226100583904 28.216714997584763 23.269031970784315 72-73 20.705001768499066 27.58665016067538 28.381703775776973 23.326644295048578 74-75 20.82992592641543 27.496084383011336 28.36243308010154 23.311556610471694 76-77 20.883280747170883 27.570044123285903 28.188333408595035 23.358341720948182 78-79 21.098134497119343 27.51552828747218 28.261109511903598 23.12522770350488 80-81 21.038604934708093 27.308684259571542 28.29391399533024 23.358796810390125 82-83 20.927130946726077 27.316977730561774 28.476990827547283 23.278900495164862 84-85 20.992420521047485 27.170525059703422 28.369062030825816 23.46799238842328 86-87 21.330053055132765 27.189152613417058 28.231169613181912 23.24962471826827 88-89 21.419657374160643 26.97428941252532 28.240233491020067 23.365819722293963 90-91 21.615890476051863 26.734114755633108 28.197790734174543 23.452204034140482 92-93 21.836551150221307 26.73118506615344 28.044105335087792 23.388158448537453 94-95 21.822310082839785 26.460796841045763 28.137598790091324 23.579294286023128 96-97 21.974698672377897 26.383783833097496 28.049941392351624 23.591576102172983 98-99 22.108280383016556 26.347378196444783 27.917775325393563 23.626566095145098 100 22.289687255998082 26.257154505868 27.839356530175547 23.61380170795837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1362.0 1 1109.5 2 1044.0 3 1286.5 4 1277.5 5 1099.0 6 901.0 7 807.5 8 796.5 9 795.5 10 760.0 11 725.5 12 764.5 13 845.0 14 955.5 15 1143.5 16 1449.5 17 1888.5 18 2588.5 19 3770.0 20 5568.5 21 8187.5 22 11724.0 23 16935.5 24 24093.0 25 33085.0 26 44856.0 27 59288.0 28 76621.0 29 95270.5 30 113276.5 31 129678.5 32 143253.0 33 156848.5 34 172057.5 35 185368.5 36 197047.0 37 208612.5 38 218737.0 39 226665.5 40 232783.0 41 235624.5 42 234504.5 43 232833.5 44 231038.5 45 227428.5 46 223367.5 47 217595.5 48 210508.0 49 205670.0 50 200472.0 51 191962.5 52 180306.5 53 169581.5 54 160652.0 55 151536.0 56 138756.5 57 123573.0 58 109809.5 59 96655.0 60 83153.0 61 69728.5 62 58283.5 63 47832.0 64 38115.5 65 30416.5 66 24167.0 67 18514.0 68 14312.0 69 11127.0 70 8569.0 71 6695.5 72 5057.5 73 3657.0 74 2675.5 75 1937.5 76 1415.0 77 1122.0 78 866.0 79 640.5 80 484.0 81 371.5 82 292.5 83 217.5 84 186.0 85 165.5 86 120.0 87 90.0 88 77.0 89 69.5 90 53.0 91 45.5 92 41.0 93 31.5 94 33.5 95 40.0 96 39.0 97 36.0 98 53.0 99 75.5 100 123.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01480134114936425 2 0.03468327017465268 3 0.03595734948719094 4 0.0385841549834118 5 0.04154127733843888 6 0.03999979866400987 7 0.0378606037688839 8 0.037498828161619945 9 0.03773476877505296 10-11 0.03804935625963031 12-13 0.039087494958735564 14-15 0.03747523410027665 16-17 0.03428217113181656 18-19 0.037019082247639494 20-21 0.03638204259137036 22-23 0.03753028691007768 24-25 0.034463058935448536 26-27 0.034502382371020705 28-29 0.035225933585548604 30-31 0.03517088077574757 32-33 0.034982128285001156 34-35 0.034785511107140316 36-37 0.032402510911466904 38-39 0.03279574526718859 40-41 0.03392826021166704 42-43 0.03378669584360724 44-45 0.032811474641417454 46-47 0.03185984750057098 48-49 0.031891306249028716 50-51 0.03314179150022367 52-53 0.03347997304614432 54-55 0.032764286518730854 56-57 0.03128572534121731 58-59 0.032087923426889556 60-61 0.03160031282579466 62-63 0.03174974188096891 64-65 0.028965642642459374 66-67 0.03019253383231103 68-69 0.031018325979326567 70-71 0.03211938217534729 72-73 0.02925663606569342 74-75 0.02976784072813161 76-77 0.030373421635943004 78-79 0.030727332556092522 80-81 0.030805979427236858 82-83 0.02942965918221096 84-85 0.030672279746291487 86-87 0.03104192004066987 88-89 0.03143515439639156 90-91 0.03188344156191428 92-93 0.030341962887485266 94-95 0.0316317715742524 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6357532.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.38732773721897 #Duplication Level Percentage of deduplicated Percentage of total 1 77.65322546465548 49.998836778449295 2 13.403011170479937 17.259681457985966 3 4.3197046481925545 8.344027167334863 4 1.7388296750602903 4.478343846692354 5 0.8977190788590439 2.8900866273225785 6 0.504707240575004 1.9498050306150119 7 0.32429516030048244 1.4616349138902787 8 0.21766042408707387 1.1211658448893194 9 0.15482937583379028 0.8972144788643375 >10 0.7316575931686923 8.162760678284513 >50 0.03881894275979221 1.7017461732322854 >100 0.015369948406002224 1.6199432246688727 >500 7.340761178922103E-5 0.02853229483671492 >1k 9.787001002698849E-5 0.08622148293359809 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.572937422886743E-5 0.0 0.0 0.0 0.0 8 1.572937422886743E-5 0.0 0.0 0.0 0.0 9 1.572937422886743E-5 0.0 0.0 6.291749691546972E-5 0.0 10-11 1.572937422886743E-5 1.572937422886743E-5 0.0 1.10105619602072E-4 0.0 12-13 1.572937422886743E-5 1.572937422886743E-5 0.0 2.9885811034848116E-4 0.0 14-15 1.572937422886743E-5 1.572937422886743E-5 0.0 3.617756072639509E-4 0.0 16-17 2.3594061343301144E-5 1.572937422886743E-5 0.0 5.033399753237577E-4 0.0 18-19 3.145874845773486E-5 1.572937422886743E-5 0.0 6.213102820402635E-4 0.0 20-21 3.932343557216857E-5 1.572937422886743E-5 0.0 8.887096439310098E-4 0.0 22-23 4.718812268660229E-5 1.572937422886743E-5 0.0 0.002147059582240404 0.0 24-25 6.291749691546972E-5 1.572937422886743E-5 0.0 0.004577247900600422 0.0 26-27 6.291749691546972E-5 1.572937422886743E-5 0.0 0.009185954549658579 0.0 28-29 7.078218402990343E-5 1.572937422886743E-5 0.0 0.032371052163009166 0.0 30-31 7.864687114433715E-5 1.572937422886743E-5 0.0 0.10356219992286315 0.0 32-33 9.437624537320458E-5 1.572937422886743E-5 0.0 0.2604469784815869 0.0 34-35 9.437624537320458E-5 1.572937422886743E-5 0.0 0.5141775141674474 0.0 36-37 9.437624537320458E-5 1.572937422886743E-5 0.0 0.9012616845656459 0.0 38-39 9.437624537320458E-5 1.572937422886743E-5 1.572937422886743E-5 1.394888771303078 0.0 40-41 9.437624537320458E-5 1.572937422886743E-5 1.572937422886743E-5 1.876545804252342 0.0 42-43 9.437624537320458E-5 1.572937422886743E-5 1.572937422886743E-5 2.333476260913826 0.0 44-45 1.0224093248763829E-4 1.572937422886743E-5 1.572937422886743E-5 2.873992612227512 0.0 46-47 1.10105619602072E-4 1.572937422886743E-5 1.572937422886743E-5 3.4919682669312557 0.0 48-49 1.2583499383093943E-4 1.572937422886743E-5 1.572937422886743E-5 4.110258509119577 0.0 50-51 1.2583499383093943E-4 1.572937422886743E-5 1.572937422886743E-5 4.696303534138719 0.0 52-53 1.2583499383093943E-4 1.572937422886743E-5 1.572937422886743E-5 5.245471041278282 0.0 54-55 1.2583499383093943E-4 1.572937422886743E-5 1.572937422886743E-5 5.820843685883139 0.0 56-57 1.4942905517424058E-4 1.572937422886743E-5 1.572937422886743E-5 6.471851026467503 0.0 58-59 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 7.140821312421235 0.0 60-61 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 7.793260026060427 0.0 62-63 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 8.407020208470833 0.0 64-65 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 9.030233744792792 0.0 66-67 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 9.703466691162546 0.0 68-69 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 10.40197674191809 0.0 70-71 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 11.099613812403934 0.0 72-73 1.572937422886743E-4 1.572937422886743E-5 1.572937422886743E-5 11.778037452269214 0.0 74-75 1.572937422886743E-4 3.145874845773486E-5 1.572937422886743E-5 12.436885885906669 0.0 76-77 1.572937422886743E-4 3.932343557216857E-5 1.572937422886743E-5 13.119540727439517 0.0 78-79 1.572937422886743E-4 5.5052809801036E-5 1.572937422886743E-5 13.841802133280652 0.0 80-81 1.572937422886743E-4 6.291749691546972E-5 1.572937422886743E-5 14.57836940498294 0.0 82-83 1.572937422886743E-4 6.291749691546972E-5 1.572937422886743E-5 15.297783794088648 0.0 84-85 1.572937422886743E-4 6.291749691546972E-5 1.572937422886743E-5 15.977701724505671 0.0 86-87 1.572937422886743E-4 7.078218402990343E-5 1.572937422886743E-5 16.670706494281113 0.0 88 1.572937422886743E-4 7.864687114433715E-5 1.572937422886743E-5 17.205623188369323 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6420 0.0 45.3246 1 GTATCAA 10670 0.0 33.08673 1 TCAACGC 11195 0.0 30.525877 4 ATCAACG 11260 0.0 30.349665 3 CAACGCA 11840 0.0 28.823692 5 AACGCAG 12795 0.0 26.890017 6 TATCAAC 12970 0.0 26.565737 2 GTACATG 16960 0.0 25.804903 1 TACATGG 17050 0.0 24.537115 2 ACGCAGA 14600 0.0 23.243666 7 CGCAGAG 14760 0.0 22.800636 8 ACATGGG 17740 0.0 22.734821 3 GTGGTAT 4070 0.0 20.559093 1 TGGTATC 3880 0.0 19.38412 2 GCAGAGT 16715 0.0 19.093418 9 GAGTACA 12795 0.0 18.627134 1 CATGGGG 11265 0.0 18.318554 4 CATGGGT 5110 0.0 17.477953 4 AGAGTAC 14300 0.0 17.239702 10-11 CAGAGTA 16135 0.0 16.983213 10-11 >>END_MODULE