FastQCFastQC Report
Wed 25 May 2016
SRR2049384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6357532
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT206490.32479584845188353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131820.20734461108493046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130910.2059132380301035No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT103570.16290912888837994TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.11410088065620433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104800.058.1412931
GTATCAA181550.038.3530351
ATCAACG221250.031.032733
TCAACGC223600.030.6855644
TATCAAC232500.029.6122
CAACGCA232750.029.4994265
AACGCAG243700.028.2595836
GTGGTAT45150.027.4907841
TGGTATC46200.025.4302772
ACGCAGA276900.024.6164637
CGCAGAG277500.024.4445768
GTACATG171500.024.2342221
TACATGG170600.023.0568242
ACATGGG172100.022.5554873
GCAGAGT301450.022.1903699
GAGTACT210100.021.17052712-13
CAGAGTA294150.020.63808310-11
AGAGTAC279600.019.52487210-11
GTACTTT232550.018.98614-15
AGTACTT222150.018.09615912-13
Produced by FastQC (version 0.11.5)