##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049384_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6357532
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782139201186876	31.0	31.0	34.0	30.0	34.0
2	31.907923861020283	33.0	31.0	34.0	30.0	34.0
3	31.884032671797797	33.0	31.0	34.0	30.0	34.0
4	35.621777759042345	37.0	35.0	37.0	33.0	37.0
5	35.733607632647384	37.0	35.0	37.0	33.0	37.0
6	35.78744660663918	37.0	35.0	37.0	35.0	37.0
7	35.72685438311596	37.0	35.0	37.0	33.0	37.0
8	35.72004136196247	37.0	35.0	37.0	33.0	37.0
9	37.437079514503424	39.0	37.0	39.0	34.0	39.0
10-11	37.409471395503786	39.0	37.0	39.0	34.0	39.0
12-13	37.362676585819784	39.0	37.0	39.0	34.0	39.0
14-15	38.62908043561558	40.0	38.0	41.0	34.0	41.0
16-17	38.65221692946257	40.0	38.0	41.0	34.0	41.0
18-19	38.728875922291856	40.0	38.0	41.0	34.0	41.0
20-21	38.767338803799966	40.0	38.0	41.0	34.0	41.0
22-23	38.72943258484582	40.0	38.0	41.0	34.0	41.0
24-25	38.70950622033833	40.0	38.0	41.0	34.0	41.0
26-27	38.588083237331716	40.0	38.0	41.0	34.0	41.0
28-29	38.49651468525837	40.0	38.0	41.0	34.0	41.0
30-31	38.392622325770446	40.0	38.0	41.0	34.0	41.0
32-33	38.25421901140254	40.0	38.0	41.0	34.0	41.0
34-35	38.149574237298374	40.0	38.0	41.0	34.0	41.0
36-37	38.02544391439949	40.0	38.0	41.0	33.0	41.0
38-39	37.91533845209116	40.0	37.0	41.0	33.0	41.0
40-41	37.82159963960858	40.0	37.0	41.0	33.0	41.0
42-43	37.69501466921441	40.0	37.0	41.0	33.0	41.0
44-45	37.52464580595112	40.0	37.0	41.0	33.0	41.0
46-47	37.30731013229662	40.0	37.0	41.0	32.0	41.0
48-49	37.10794314523309	40.0	37.0	41.0	31.0	41.0
50-51	36.95788530832405	40.0	37.0	41.0	31.0	41.0
52-53	36.828800547130555	40.0	36.0	41.0	31.0	41.0
54-55	36.680365509760705	39.5	36.0	41.0	31.0	41.0
56-57	36.54040254929114	39.0	35.5	41.0	31.0	41.0
58-59	36.34136666555513	39.0	35.0	41.0	30.0	41.0
60-61	36.13758499367364	39.0	35.0	41.0	30.0	41.0
62-63	35.921173735342585	39.0	35.0	41.0	29.5	41.0
64-65	35.66985349031668	38.0	35.0	40.0	29.0	41.0
66-67	35.353151741902366	38.0	35.0	40.0	29.0	41.0
68-69	35.00826523562917	37.0	34.5	40.0	28.5	41.0
70-71	34.63477470502704	37.0	34.0	39.5	28.0	41.0
72-73	34.32507347190702	36.0	34.0	39.0	28.0	41.0
74-75	34.00752768527158	36.0	34.0	39.0	28.0	41.0
76-77	33.667930810257815	35.0	34.0	38.5	27.0	40.0
78-79	33.31582554362291	35.0	34.0	37.0	26.5	39.0
80-81	32.97660672411873	35.0	33.0	37.0	26.0	39.0
82-83	32.663323991133666	35.0	33.0	36.5	26.0	39.0
84-85	32.38300255507955	35.0	33.0	36.0	26.0	37.0
86-87	32.14154918921368	35.0	33.0	36.0	26.0	37.0
88-89	31.90052381962057	35.0	33.0	35.0	25.0	37.0
90-91	31.687219663227808	35.0	33.0	35.0	25.0	36.0
92-93	31.490665088276394	35.0	33.0	35.0	25.0	36.0
94-95	31.323626133537353	35.0	33.0	35.0	24.5	36.0
96-97	31.11184686132921	35.0	32.5	35.0	23.5	36.0
98-99	30.944177551917946	35.0	32.5	35.0	23.0	35.0
100	30.815856530490134	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17885271028753635
1101	2	0.21782296759319308
1101	3	0.37919454661069807
1101	4	0.2436972499300225
1101	5	0.18516521686839837
1101	6	0.17704499048715405
1101	7	0.08961978546744831
1101	8	0.15698364483568383
1101	9	0.2631446651157816
1101	10-11	0.2685563585176993
1101	12-13	0.20109434205385668
1101	14-15	0.30653791195750557
1101	16-17	0.2012718033708012
1101	18-19	0.27064928054275583
1101	20-21	0.27678139817386693
1101	22-23	0.30902909162428216
1101	24-25	0.2873097632606587
1101	26-27	0.3623307832667777
1101	28-29	0.38670925291962277
1101	30-31	0.3782162839125718
1101	32-33	0.40839570513859513
1101	34-35	0.4010940229875999
1101	36-37	0.399992507672998
1101	38-39	0.43953081870138533
1101	40-41	0.4718218274852717
1101	42-43	0.4720349547521465
1101	44-45	0.47261496661882063
1101	46-47	0.4999058361437676
1101	48-49	0.5383423068096391
1101	50-51	0.5631250649665489
1101	52-53	0.6072606983081386
1101	54-55	0.5374476301646567
1101	56-57	0.5117216972863758
1101	58-59	0.5342146396767546
1101	60-61	0.5243413511592081
1101	62-63	0.4993767558800144
1101	64-65	0.4644517891390052
1101	66-67	0.4556780491591823
1101	68-69	0.5080805470699374
1101	70-71	0.5200796265420919
1101	72-73	0.4446946324046692
1101	74-75	0.5729093977360193
1101	76-77	0.5168298353572425
1101	78-79	0.5303904043407499
1101	80-81	0.5601621102960337
1101	82-83	0.48317988557111846
1101	84-85	0.5717642806606662
1101	86-87	0.47677145453116054
1101	88-89	0.5532441650452213
1101	90-91	0.617297990960882
1101	92-93	0.5784656317247752
1101	94-95	0.6121164515585882
1101	96-97	0.6225189731085514
1101	98-99	0.6687754869213514
1101	100	0.6992078953486249
1102	1	0.2477744290256041
1102	2	0.2103038101153487
1102	3	0.359252224445882
1102	4	0.23697888329449768
1102	5	0.22983119177553135
1102	6	0.21894860841749875
1102	7	0.18705961459426845
1102	8	0.17781399776691842
1102	9	0.16039199417238592
1102	10-11	0.24046862037974392
1102	12-13	0.2247494730659838
1102	14-15	0.2481964063069242
1102	16-17	0.19625034316320722
1102	18-19	0.23963173386014347
1102	20-21	0.23380398894368426
1102	22-23	0.23521613601650415
1102	24-25	0.2543857676485075
1102	26-27	0.32630636448783434
1102	28-29	0.29506827349102593
1102	30-31	0.25235819060168296
1102	32-33	0.285285052575567
1102	34-35	0.2794996612040137
1102	36-37	0.3683665455335685
1102	38-39	0.2528933580066024
1102	40-41	0.27775060521548056
1102	42-43	0.3036192131366633
1102	44-45	0.30005351083687515
1102	46-47	0.19678475468894874
1102	48-49	0.1826849611345267
1102	50-51	0.25194671962979953
1102	52-53	0.3101527864463236
1102	54-55	0.2002420074878941
1102	56-57	0.2471069691082377
1102	58-59	0.2223841473611401
1102	60-61	0.16697364623200883
1102	62-63	0.23333541804193203
1102	64-65	0.2062324416941692
1102	66-67	0.15784193920669054
1102	68-69	0.25622257424385
1102	70-71	0.2837940457908914
1102	72-73	0.23617184174557337
1102	74-75	0.24054897045857615
1102	76-77	0.20439063884127506
1102	78-79	0.24932967737375833
1102	80-81	0.18956698491702184
1102	82-83	0.25396132928358384
1102	84-85	0.23595007374336774
1102	86-87	0.23873508386949993
1102	88-89	0.21415531104472052
1102	90-91	0.2542899893523156
1102	92-93	0.34378224295705806
1102	94-95	0.3251524022296408
1102	96-97	0.414777194359079
1102	98-99	0.4342660202017967
1102	100	0.4352264342590466
1103	1	0.2101593081590707
1103	2	0.1895179277705168
1103	3	0.3402163951326642
1103	4	0.21267076825598252
1103	5	0.17509163045031073
1103	6	0.1402988090991215
1103	7	0.12767960856199778
1103	8	0.11442452058135189
1103	9	0.13939296700237946
1103	10-11	0.16084632422412426
1103	12-13	0.21332869962233048
1103	14-15	0.2390296827626699
1103	16-17	0.2230949809396492
1103	18-19	0.21676775232964474
1103	20-21	0.23193699164145443
1103	22-23	0.22109872349109594
1103	24-25	0.2652125542126811
1103	26-27	0.2874766424741253
1103	28-29	0.3204037933545081
1103	30-31	0.26340483541184767
1103	32-33	0.2600578903414359
1103	34-35	0.2752843558139588
1103	36-37	0.33279941403458935
1103	38-39	0.3269366505945399
1103	40-41	0.30289290679313297
1103	42-43	0.3174472382515461
1103	44-45	0.34035389945719174
1103	46-47	0.3032105343456095
1103	48-49	0.19642691868581608
1103	50-51	0.21167706313907075
1103	52-53	0.262178794389456
1103	54-55	0.2468235430335497
1103	56-57	0.2875781665151038
1103	58-59	0.2742937126544476
1103	60-61	0.2823887843749091
1103	62-63	0.2723693273670733
1103	64-65	0.3051632087373193
1103	66-67	0.2821033041952532
1103	68-69	0.27541267394456526
1103	70-71	0.3093143481623528
1103	72-73	0.3238641663229771
1103	74-75	0.2782424019960956
1103	76-77	0.26792806900991195
1103	78-79	0.25964261694802815
1103	80-81	0.2865804040017821
1103	82-83	0.30971550347555876
1103	84-85	0.3214253476675992
1103	86-87	0.31547356347907396
1103	88-89	0.33743468737446847
1103	90-91	0.3537882918940376
1103	92-93	0.3840021711030879
1103	94-95	0.3524383782795013
1103	96-97	0.3965085682401579
1103	98-99	0.4278099723204747
1103	100	0.47834830770173653
1104	1	0.2044655897423695
1104	2	0.20725617439367028
1104	3	0.2924425265094257
1104	4	0.20884264772861627
1104	5	0.1758750256893009
1104	6	0.2005616039860243
1104	7	0.21261729430499798
1104	8	0.17657923963258781
1104	9	0.2195940662219371
1104	10-11	0.224464982899093
1104	12-13	0.1899317045912312
1104	14-15	0.26243992473477107
1104	16-17	0.2330010290607234
1104	18-19	0.27937258603486015
1104	20-21	0.3248956911871588
1104	22-23	0.2520306899077269
1104	24-25	0.23860226312692134
1104	26-27	0.2927758079659242
1104	28-29	0.3094559957359735
1104	30-31	0.33987979100736254
1104	32-33	0.37403308627345666
1104	34-35	0.38846859623292573
1104	36-37	0.36989511087026017
1104	38-39	0.3399356888414786
1104	40-41	0.36313779167213056
1104	42-43	0.3935104073283142
1104	44-45	0.3485612383989931
1104	46-47	0.373579853314439
1104	48-49	0.3746008312795652
1104	50-51	0.3268337449404086
1104	52-53	0.2551565209241744
1104	54-55	0.26197974356328757
1104	56-57	0.23821895690770134
1104	58-59	0.32786884953132045
1104	60-61	0.32457540694464626
1104	62-63	0.32858388581390585
1104	64-65	0.25022494187680877
1104	66-67	0.311598884386008
1104	68-69	0.2751896787825885
1104	70-71	0.3304691562608113
1104	72-73	0.24792944408768847
1104	74-75	0.27941317897366247
1104	76-77	0.2751789165567402
1104	78-79	0.2858611505546378
1104	80-81	0.2915388962415406
1104	82-83	0.33493101079365317
1104	84-85	0.3191056784012716
1104	86-87	0.3139046479834491
1104	88-89	0.2751768181707206
1104	90-91	0.3024919221831972
1104	92-93	0.3821623308220481
1104	94-95	0.4692845927754554
1104	96-97	0.48304984617525193
1104	98-99	0.5280487428951659
1104	100	0.4918473177068954
1105	1	0.19309368666021953
1105	2	0.179599048128253
1105	3	0.275500283608757
1105	4	0.16805692180653153
1105	5	0.13829286458204848
1105	6	0.11115926870047588
1105	7	0.17163493742170743
1105	8	0.16304542504418862
1105	9	0.16889707912437757
1105	10-11	0.19534523575194385
1105	12-13	0.18561492672991164
1105	14-15	0.2458858380008877
1105	16-17	0.24573308169638608
1105	18-19	0.23486761397878553
1105	20-21	0.23671731239202387
1105	22-23	0.24296955683526278
1105	24-25	0.22617647667036067
1105	26-27	0.2780704797259972
1105	28-29	0.27381034087650136
1105	30-31	0.21301292489167167
1105	32-33	0.22807053576407554
1105	34-35	0.28648358134865504
1105	36-37	0.25723554413281136
1105	38-39	0.2938513759550716
1105	40-41	0.31158066285779284
1105	42-43	0.30297736485681526
1105	44-45	0.2880824546738907
1105	46-47	0.2892241418895338
1105	48-49	0.324571669103193
1105	50-51	0.3188185001831485
1105	52-53	0.29930076540770756
1105	54-55	0.27584871736656424
1105	56-57	0.25167412702751335
1105	58-59	0.28099409442948087
1105	60-61	0.29040385102221933
1105	62-63	0.3140947803882099
1105	64-65	0.28099839973675245
1105	66-67	0.29877093061929827
1105	68-69	0.29948638667051597
1105	70-71	0.3155486342736822
1105	72-73	0.38825706103284574
1105	74-75	0.3535093691933753
1105	76-77	0.3190621342308546
1105	78-79	0.2841902227588875
1105	80-81	0.3506987326737061
1105	82-83	0.36566457251653617
1105	84-85	0.32526733596544943
1105	86-87	0.3017453898869462
1105	88-89	0.3342121770721036
1105	90-91	0.3501917911433914
1105	92-93	0.4271073477707823
1105	94-95	0.44685912453808285
1105	96-97	0.44020836096872173
1105	98-99	0.4181831722957057
1105	100	0.4797032977182951
1106	1	0.11691861009744287
1106	2	0.1375757471524821
1106	3	0.26383632168250415
1106	4	0.18071935467394695
1106	5	0.1103068270368297
1106	6	0.11720049965395418
1106	7	0.11600100744469444
1106	8	0.08476510315840358
1106	9	0.1557577152704468
1106	10-11	0.14916946732603265
1106	12-13	0.14417969853840162
1106	14-15	0.18161793576512508
1106	16-17	0.20693911875355298
1106	18-19	0.13696281521524867
1106	20-21	0.15111108760745395
1106	22-23	0.12560858706805078
1106	24-25	0.11839633224170854
1106	26-27	0.23401622552029977
1106	28-29	0.2566211906736484
1106	30-31	0.27275637177770307
1106	32-33	0.21075021757750534
1106	34-35	0.2265517860902051
1106	36-37	0.21479715246729114
1106	38-39	0.2623344548447406
1106	40-41	0.28521976960659146
1106	42-43	0.29743617955629986
1106	44-45	0.2182214937153759
1106	46-47	0.32558280534481554
1106	48-49	0.34183833037879907
1106	50-51	0.31669894888803896
1106	52-53	0.3073802412325364
1106	54-55	0.33297824401865483
1106	56-57	0.34879531630478766
1106	58-59	0.3808015004838907
1106	60-61	0.36723151589911396
1106	62-63	0.3746655050470835
1106	64-65	0.31240667484607343
1106	66-67	0.29478692672972784
1106	68-69	0.3552463244763473
1106	70-71	0.36197169319958533
1106	72-73	0.386357324723825
1106	74-75	0.3121359432363704
1106	76-77	0.2957902325868673
1106	78-79	0.3466585304903802
1106	80-81	0.3529199917636845
1106	82-83	0.4141777769233528
1106	84-85	0.37712359212419244
1106	86-87	0.38632856638564306
1106	88-89	0.39159196308042254
1106	90-91	0.38544039055011226
1106	92-93	0.4544306289411324
1106	94-95	0.44651378022014043
1106	96-97	0.4284177060929757
1106	98-99	0.43557555865191233
1106	100	0.42845237829723004
1107	1	0.1119746642845385
1107	2	0.08057607902189545
1107	3	0.19291514852007197
1107	4	0.14585437454845618
1107	5	0.13400437361588757
1107	6	0.14834806571582249
1107	7	0.09346388681422724
1107	8	0.15073362845692628
1107	9	0.1388848958097526
1107	10-11	0.1138264222626475
1107	12-13	0.09509166284578185
1107	14-15	0.15977471859687142
1107	16-17	0.12772906647613524
1107	18-19	0.14979469154723546
1107	20-21	0.1432781406466077
1107	22-23	0.11635874833258697
1107	24-25	0.12467996306249063
1107	26-27	0.18332081568838277
1107	28-29	0.17259349998138873
1107	30-31	0.14628202838149207
1107	32-33	0.1751240313911424
1107	34-35	0.15197746957461078
1107	36-37	0.16167710441835226
1107	38-39	0.18576251230823715
1107	40-41	0.14599299066800597
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2216	78-79	-0.9367152225878641
2216	80-81	-1.0730824172771705
2216	82-83	-0.949430578444737
2216	84-85	-1.0902846814231175
2216	86-87	-0.9257418317746371
2216	88-89	-1.2725673150885868
2216	90-91	-1.0634730720073868
2216	92-93	-1.159840611509786
2216	94-95	-1.092195616596598
2216	96-97	-1.1246001474751743
2216	98-99	-1.3248133255731993
2216	100	-1.2518288512024647
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	334.0
3	47.0
4	50.0
5	105.0
6	293.0
7	847.0
8	2041.0
9	3577.0
10	5618.0
11	7898.0
12	11129.0
13	15236.0
14	18313.0
15	22929.0
16	28274.0
17	37981.0
18	37670.0
19	23150.0
20	19673.0
21	20136.0
22	22693.0
23	25891.0
24	30186.0
25	36632.0
26	44668.0
27	48600.0
28	60886.0
29	80646.0
30	104267.0
31	134691.0
32	173140.0
33	225023.0
34	304299.0
35	432232.0
36	688216.0
37	1241465.0
38	1742390.0
39	702920.0
40	3386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.84726317563426	21.127607169084587	14.627416220713974	23.397713434567176
2	15.352089458613813	20.741775267509468	40.21752466208585	23.688610611790864
3	17.587532394646225	25.741152384290007	31.388186484944157	25.283128736119615
4	12.244252091614197	17.663337805720147	38.42117929447443	31.671230808191233
5	12.283241358439092	38.0117001377264	36.20456806194605	13.500490441888454
6	30.08218127446016	38.75642918611014	17.30226718516984	13.859122354259853
7	25.78650017019183	33.45622169105873	22.618682847369072	18.138595291380366
8	24.513788886310998	36.68236210130594	20.527929720525837	18.275919291857225
9	24.285650469395986	16.528284875325834	21.941297346202898	37.244767309075286
10-11	23.362045208738234	27.259312261424718	29.04170203154306	20.33694049829399
12-13	24.017282363181884	24.739497377250633	29.35394216536095	21.889278094206535
14-15	21.34967617115151	26.886634342767035	26.857464186141623	24.906225299939834
16-17	20.33701128047802	30.386587122172564	27.664123436578848	21.61227816077056
18-19	20.18350369959018	28.55807095477433	30.532234342323434	20.72619100331206
20-21	21.269350188520587	27.733725456362485	30.33033198356877	20.666592371548155
22-23	20.87236839704464	27.84177098911968	30.387530884626297	20.898329729209387
24-25	20.915409979641442	27.925309904502114	30.35884415990316	20.800435955953287
26-27	20.655166856979182	27.933973682056013	30.463464126097488	20.947395334867316
28-29	20.441736149500773	27.697577296629944	30.73851843305523	21.122168120814052
30-31	20.945377934816367	27.98543742960613	30.457300919389862	20.61188371618764
32-33	20.48719630352687	27.971530405844607	30.68078674394943	20.860486546679095
34-35	20.64738055420283	27.673963842550126	30.865157023295847	20.8134985799512
36-37	21.050727908938164	27.83334271853548	30.165460589543798	20.95046878298256
38-39	20.810411623418602	27.60852912539658	30.484702344156755	21.096356907028067
40-41	20.602691990563624	27.36035212439556	30.748092890221763	21.288862994819056
42-43	20.687784722857955	27.29996139990921	30.73800416453139	21.274249712701444
44-45	20.536440858521182	27.565210832820096	30.526903324703913	21.371444983954813
46-47	20.77291870657213	27.639191351980525	30.03205649997444	21.5558334414729
48-49	20.675198402134054	27.63431639850353	29.80479134852106	21.88569385084136
50-51	20.501958316948485	27.64273692489186	29.76378293354306	22.091521824616596
52-53	20.37690013972489	27.7095861529065	29.995314190672328	21.918199516696284
54-55	20.48047027286903	27.987335588884342	29.52823991126701	22.003954226979612
56-57	20.516752477490144	27.80436626547338	29.258744701625684	22.4201365554108
58-59	20.24481393851252	27.703050024836724	29.60388497279286	22.4482510638579
60-61	20.50346353555104	28.22677266815163	28.83403766971434	22.435726126582985
62-63	20.17129931512834	28.292755205944005	28.85135805234426	22.6845874265834
64-65	20.165503787810866	28.070288028403628	28.833174623098586	22.931033560686924
66-67	20.211747547632854	28.13193824107198	28.58420440086708	23.072109810428085
68-69	20.63059511856897	28.121418074164772	28.352580339347337	22.89540646791892
70-71	20.59628923435651	27.8941162739705	28.254934092038038	23.25466039963495
72-73	20.414877693369558	27.855320926709144	28.398399110085403	23.331402269835895
74-75	20.506653122486224	27.796727501177354	28.340497613357275	23.35612176297915
76-77	20.547229405361872	27.803941184242813	28.192617200525994	23.45621220986932
78-79	20.713271417903197	27.786974951345517	28.16394153046229	23.335812100288997
80-81	20.585999816905534	27.582583626509937	28.230020760144047	23.60139579644048
82-83	20.393381148644956	27.542067787425694	28.44083258257343	23.623718481355922
84-85	20.451704618070778	27.329100708037334	28.350782262298242	23.868412411593646
86-87	20.595671688862428	27.49838181848211	28.24465646183333	23.66129003082213
88-89	20.507469595890964	27.252092068061412	28.308647293302275	23.931791042745346
90-91	20.45924562560201	26.943398915320284	28.356461773718827	24.240893685358884
92-93	20.4488578981324	26.953613513240793	28.37045515373119	24.227073434895622
94-95	20.292714954483117	26.672472056188536	28.696865703125113	24.33794728620324
96-97	20.364003869536834	26.70563206367433	28.62220893925928	24.308155127529552
98-99	20.37824372270485	26.74897394451404	28.527063766760897	24.345718566020214
100	20.421347850672948	26.752749331296876	28.481585791798764	24.34431702623141
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	567.0
1	424.5
2	476.0
3	644.0
4	421.5
5	331.0
6	836.5
7	2045.5
8	3455.5
9	4049.5
10	3269.5
11	2337.5
12	2320.5
13	2717.0
14	3218.5
15	3711.5
16	4334.0
17	5123.0
18	6085.0
19	7314.0
20	9199.0
21	11880.0
22	15365.0
23	20188.0
24	26862.0
25	35521.5
26	46865.5
27	60638.5
28	77038.5
29	95352.5
30	112555.0
31	128199.5
32	141891.0
33	155115.0
34	169669.5
35	182398.0
36	194104.5
37	206265.5
38	216665.5
39	225147.0
40	232573.5
41	237149.0
42	238495.5
43	239728.0
44	239130.5
45	235802.0
46	231928.5
47	225639.5
48	217096.5
49	210016.0
50	202820.5
51	192819.5
52	180014.0
53	167357.5
54	156081.5
55	145275.0
56	131776.5
57	115998.0
58	102155.5
59	90022.5
60	77496.0
61	64564.5
62	53828.5
63	44058.0
64	34601.5
65	27103.5
66	21262.0
67	16051.5
68	11939.0
69	8917.5
70	6646.5
71	4814.5
72	3283.0
73	2284.0
74	1593.0
75	1053.5
76	672.5
77	450.0
78	305.5
79	173.0
80	106.5
81	58.5
82	23.5
83	13.0
84	10.5
85	6.5
86	3.0
87	2.0
88	1.0
89	2.5
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03792352126579937
2	0.0
3	0.0
4	3.145874845773486E-5
5	0.0
6	1.572937422886743E-5
7	0.0
8	8.965743310454435E-4
9	0.0
10-11	0.0
12-13	0.04475793436824227
14-15	1.1010561960207199E-4
16-17	0.0
18-19	5.5052809801035996E-5
20-21	5.5052809801035996E-5
22-23	0.0
24-25	1.4156436805980684E-4
26-27	1.0224093248763829E-4
28-29	4.168284170649869E-4
30-31	7.864687114433715E-6
32-33	1.9661717786084287E-4
34-35	6.291749691546972E-5
36-37	9.437624537320458E-5
38-39	3.4604623303508344E-4
40-41	9.437624537320458E-5
42-43	5.347987237814926E-4
44-45	2.9099342323404744E-4
46-47	1.1010561960207199E-4
48-49	8.2579214701554E-4
50-51	5.033399753237577E-4
52-53	6.134455949258297E-4
54-55	4.95475288209324E-4
56-57	6.213102820402635E-4
58-59	1.572937422886743E-4
60-61	1.7302311651754172E-4
62-63	9.988152635330818E-4
64-65	5.347987237814926E-4
66-67	0.001620125545573345
68-69	0.001462831803284671
70-71	4.718812268660229E-4
72-73	0.0010695974475629852
74-75	0.0010381386991052503
76-77	8.179274599011063E-4
78-79	0.0032166570298033893
80-81	0.002485241128161054
82-83	0.0013291321223392976
84-85	0.0026661289317930293
86-87	0.0013684555579114661
88-89	0.0019897658399517294
90-91	0.0026975876802507637
92-93	0.002398729569902283
94-95	0.0034761917045797014
96-97	0.002862746109653872
98-99	0.0027683698642806676
100	0.0012111618156227918
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6357532.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.61373697993244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.65588051869105	47.23055260243758
2	13.886740328345242	17.11227932198569
3	4.388463679885357	8.111689407553285
4	1.9375609404474576	4.775214806692808
5	0.9627061533530096	2.965796186082742
6	0.5471741652820742	2.022806706114228
7	0.3480836535696047	1.5012714274636083
8	0.25041638876486716	1.2343271610258428
9	0.1636529039425356	0.9074940281565783
>10	0.7938153515040449	8.544721772840088
>50	0.04523963521377101	1.8873110073591917
>100	0.019218121619533995	1.9804555198132074
>500	5.624425783354906E-4	0.2416306619442023
>1k	3.3233254959038586E-4	0.38570955163826715
>5k	5.1128084493664895E-5	0.1965405007339961
>10k+	1.0225616898732979E-4	0.9021993381585642
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	20649	0.32479584845188353	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13182	0.20734461108493046	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13091	0.2059132380301035	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	10357	0.16290912888837994	TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7254	0.11410088065620433	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.572937422886743E-5	0.0	0.0	0.0	0.0
3	1.572937422886743E-5	0.0	0.0	0.0	0.0
4	1.572937422886743E-5	0.0	0.0	1.572937422886743E-5	0.0
5	1.572937422886743E-5	0.0	0.0	1.572937422886743E-5	0.0
6	1.572937422886743E-5	0.0	0.0	1.572937422886743E-5	0.0
7	1.572937422886743E-5	0.0	0.0	1.572937422886743E-5	0.0
8	1.572937422886743E-5	0.0	0.0	1.572937422886743E-5	0.0
9	1.572937422886743E-5	0.0	0.0	1.10105619602072E-4	0.0
10-11	1.572937422886743E-5	0.0	0.0	1.4156436805980686E-4	0.0
12-13	2.3594061343301144E-5	0.0	0.0	3.30316858806216E-4	0.0
14-15	4.718812268660229E-5	0.0	0.0	3.617756072639509E-4	0.0
16-17	4.718812268660229E-5	0.0	0.0	5.033399753237577E-4	0.0
18-19	6.291749691546972E-5	0.0	0.0	6.134455949258297E-4	0.0
20-21	6.291749691546972E-5	0.0	0.0	8.80844956816576E-4	0.0
22-23	7.078218402990343E-5	0.0	0.0	0.0021077361466682355	0.0
24-25	7.864687114433715E-5	0.0	0.0	0.004522195090799386	0.0
26-27	7.864687114433715E-5	0.0	0.0	0.009138766426971976	0.0
28-29	7.864687114433715E-5	0.0	0.0	0.032371052163009166	0.0
30-31	7.864687114433715E-5	0.0	0.0	0.10404194583684362	0.0
32-33	7.864687114433715E-5	0.0	0.0	0.26172105779412513	0.0
34-35	7.864687114433715E-5	0.0	0.0	0.5167178081054095	0.0
36-37	7.864687114433715E-5	0.0	0.0	0.9055400743558979	0.0
38-39	7.864687114433715E-5	0.0	0.0	1.4010389566265653	0.0
40-41	7.864687114433715E-5	0.0	0.0	1.8848273197838408	0.0
42-43	7.864687114433715E-5	0.0	0.0	2.344148641328113	0.0
44-45	8.651155825877086E-5	0.0	0.0	2.8874019037576213	0.0
46-47	9.437624537320458E-5	0.0	0.0	3.5084762451844522	0.0
48-49	1.10105619602072E-4	0.0	0.0	4.1303921081325266	0.0
50-51	1.10105619602072E-4	0.0	0.0	4.718961697715402	0.0
52-53	1.10105619602072E-4	0.0	0.0	5.270111106007803	0.0
54-55	1.10105619602072E-4	0.0	0.0	5.84851951983883	0.0
56-57	1.10105619602072E-4	0.0	0.0	6.503765926777875	0.0
58-59	1.10105619602072E-4	0.0	0.0	7.177360648754894	0.0
60-61	1.10105619602072E-4	0.0	0.0	7.83418785780394	0.0
62-63	1.10105619602072E-4	0.0	0.0	8.452320806249972	0.0
64-65	1.10105619602072E-4	0.0	0.0	9.08030820765039	0.0
66-67	1.10105619602072E-4	0.0	0.0	9.758299289724377	0.0
68-69	1.10105619602072E-4	0.0	0.0	10.462786502686892	0.0
70-71	1.10105619602072E-4	0.0	0.0	11.166416464753933	0.0
72-73	1.10105619602072E-4	0.0	0.0	11.851643058973199	0.0
74-75	1.10105619602072E-4	0.0	0.0	12.517034912289862	0.0
76-77	1.10105619602072E-4	0.0	0.0	13.206634272544754	0.0
78-79	1.10105619602072E-4	0.0	0.0	13.935730091488331	0.0
80-81	1.10105619602072E-4	0.0	0.0	14.67940704034207	0.0
82-83	1.10105619602072E-4	0.0	0.0	15.405356984439873	0.0
84-85	1.10105619602072E-4	0.0	0.0	16.091676770168046	0.0
86-87	1.10105619602072E-4	0.0	0.0	16.79195637552434	0.0
88	1.10105619602072E-4	0.0	0.0	17.333219872113897	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10480	0.0	58.141293	1
GTATCAA	18155	0.0	38.353035	1
ATCAACG	22125	0.0	31.03273	3
TCAACGC	22360	0.0	30.685564	4
TATCAAC	23250	0.0	29.612	2
CAACGCA	23275	0.0	29.499426	5
AACGCAG	24370	0.0	28.259583	6
GTGGTAT	4515	0.0	27.490784	1
TGGTATC	4620	0.0	25.430277	2
ACGCAGA	27690	0.0	24.616463	7
CGCAGAG	27750	0.0	24.444576	8
GTACATG	17150	0.0	24.234222	1
TACATGG	17060	0.0	23.056824	2
ACATGGG	17210	0.0	22.555487	3
GCAGAGT	30145	0.0	22.190369	9
GAGTACT	21010	0.0	21.170527	12-13
CAGAGTA	29415	0.0	20.638083	10-11
AGAGTAC	27960	0.0	19.524872	10-11
GTACTTT	23255	0.0	18.986	14-15
AGTACTT	22215	0.0	18.096159	12-13
>>END_MODULE