FastQCFastQC Report
Wed 25 May 2016
SRR2049383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614219
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44150.044.8271061
GTATCAA75300.037.2707981
TCAACGC81250.033.3242344
ATCAACG82700.032.7399523
CAACGCA86550.031.2839225
AACGCAG91550.029.6234866
TATCAAC93550.029.0934932
GTACATG123100.025.777161
ACGCAGA107850.024.8409777
CGCAGAG108550.024.507598
TACATGG126050.023.7924372
ACATGGG127750.022.4087433
GCAGAGT128150.019.695629
CATGGGG93200.019.2667544
GTATAGG15550.018.7434981
GAGTACA92550.018.184271
AGAGTAC110400.017.68951410-11
TATACTG30350.017.0372625
CAGAGTA124150.016.9418310-11
GTGGTAT29050.016.8297061
Produced by FastQC (version 0.11.5)