##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049383_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4614219 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.66310398357772 31.0 31.0 34.0 28.0 34.0 2 30.912193374436715 31.0 31.0 34.0 28.0 34.0 3 31.094456938433133 31.0 31.0 34.0 28.0 34.0 4 34.72325154050989 37.0 35.0 37.0 32.0 37.0 5 34.75947153787022 37.0 35.0 37.0 32.0 37.0 6 34.75687044763155 37.0 35.0 37.0 32.0 37.0 7 34.665659345601064 37.0 35.0 37.0 32.0 37.0 8 34.63303063855443 37.0 35.0 37.0 32.0 37.0 9 36.24736125441814 39.0 37.0 39.0 32.0 39.0 10-11 36.2100740125252 39.0 37.0 39.0 32.0 39.0 12-13 36.14824480589239 39.0 37.0 39.0 32.0 39.0 14-15 37.34992736582291 40.0 38.0 41.0 32.0 41.0 16-17 37.31570954911329 40.0 38.0 41.0 31.5 41.0 18-19 37.34414513051938 40.0 38.0 41.0 32.0 41.0 20-21 37.41364378240391 40.0 38.0 41.0 32.0 41.0 22-23 37.36554452660353 40.0 38.0 41.0 32.0 41.0 24-25 37.3649951378554 40.0 38.0 41.0 32.0 41.0 26-27 37.346631900219734 40.0 38.0 41.0 32.0 41.0 28-29 37.28843971211596 40.0 37.5 41.0 31.0 41.0 30-31 37.225301941672036 40.0 37.0 41.0 31.0 41.0 32-33 37.164996503200214 40.0 37.0 41.0 31.0 41.0 34-35 37.06603815293552 40.0 37.0 41.0 31.0 41.0 36-37 36.99020094191455 40.0 37.0 41.0 30.0 41.0 38-39 36.89040583032578 40.0 37.0 41.0 30.0 41.0 40-41 36.78760966915528 40.0 37.0 41.0 30.0 41.0 42-43 36.655523827542645 40.0 37.0 41.0 30.0 41.0 44-45 36.533115246588864 40.0 36.0 41.0 30.0 41.0 46-47 36.41060220592044 40.0 36.0 41.0 30.0 41.0 48-49 36.285212947196484 40.0 36.0 41.0 29.5 41.0 50-51 36.15096931896817 40.0 36.0 41.0 29.0 41.0 52-53 36.010072993934614 39.0 35.0 41.0 29.0 41.0 54-55 35.878542825990706 39.0 35.0 41.0 28.0 41.0 56-57 35.7543157357724 39.0 35.0 41.0 28.0 41.0 58-59 35.55748654322649 39.0 35.0 41.0 28.0 41.0 60-61 35.34223418957791 39.0 35.0 40.0 28.0 41.0 62-63 35.13676225597441 38.0 34.5 40.0 28.0 41.0 64-65 34.89901183710613 38.0 34.0 40.0 27.0 41.0 66-67 34.63641355124237 37.5 34.0 40.0 26.5 41.0 68-69 34.35319053993753 37.0 34.0 40.0 26.0 41.0 70-71 34.068244485144724 36.5 34.0 39.5 26.0 41.0 72-73 33.726188982360824 36.0 34.0 39.0 26.0 41.0 74-75 33.37820972519943 36.0 33.0 39.0 26.0 40.5 76-77 33.044060327435695 35.0 33.0 38.5 25.0 40.0 78-79 32.7251390755402 35.0 33.0 37.0 25.0 39.0 80-81 32.372612353249814 35.0 33.0 37.0 24.5 39.0 82-83 32.06044771607069 35.0 33.0 36.5 24.0 39.0 84-85 31.77062261240743 35.0 32.5 36.0 23.5 37.5 86-87 31.455347806421845 35.0 32.0 36.0 22.5 37.0 88-89 31.208209124881158 35.0 32.0 35.0 20.5 37.0 90-91 31.007432785483307 35.0 32.0 35.0 20.0 36.0 92-93 30.7751787463924 35.0 32.0 35.0 19.0 36.0 94-95 30.581314627675887 35.0 32.0 35.0 17.5 36.0 96-97 30.34014228626773 35.0 32.0 35.0 6.5 36.0 98-99 30.145561578243253 35.0 32.0 35.0 2.0 35.0 100 30.028069105519265 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21680835392212572 1101 2 0.2072044486590201 1101 3 0.26509665161944085 1101 4 0.3020694294102597 1101 5 0.3396932987276813 1101 6 0.39780895610092415 1101 7 0.3818512486265817 1101 8 0.42686800449200035 1101 9 0.5611183930555512 1101 10-11 0.5211302947700602 1101 12-13 0.4982677978687704 1101 14-15 0.5765315759250953 1101 16-17 0.6359715571347593 1101 18-19 0.6138559729698159 1101 20-21 0.5667183400678866 1101 22-23 0.5320104255816602 1101 24-25 0.5453162506777574 1101 26-27 0.4561589278593914 1101 28-29 0.5204494220974141 1101 30-31 0.49464167881208 1101 32-33 0.4764422676088955 1101 34-35 0.5255269444796227 1101 36-37 0.4945096908674955 1101 38-39 0.562176877936615 1101 40-41 0.6243123196711551 1101 42-43 0.5505325350927777 1101 44-45 0.5231705988216788 1101 46-47 0.5303991640953498 1101 48-49 0.4821077778203815 1101 50-51 0.477620547123351 1101 52-53 0.4741853595068122 1101 54-55 0.4876534247139972 1101 56-57 0.4710647871978324 1101 58-59 0.5379136203183847 1101 60-61 0.5609287752177892 1101 62-63 0.5723187082495969 1101 64-65 0.5374168746964187 1101 66-67 0.5916240748755612 1101 68-69 0.5338071314754487 1101 70-71 0.5713738986634453 1101 72-73 0.6140853484997564 1101 74-75 0.6202207778934365 1101 76-77 0.5330259401265351 1101 78-79 0.48889956828548975 1101 80-81 0.5604970405287872 1101 82-83 0.6221095808773995 1101 84-85 0.5699571770412319 1101 86-87 0.53608353763358 1101 88-89 0.5339097975402503 1101 90-91 0.5950050151832507 1101 92-93 0.5637252781434547 1101 94-95 0.5679447461352076 1101 96-97 0.48491642185647876 1101 98-99 0.5954290635300552 1101 100 0.5808410015898815 1102 1 0.12256631378931004 1102 2 0.18445837467146475 1102 3 0.26352135779067254 1102 4 0.2696117091188768 1102 5 0.25896273633289724 1102 6 0.3567395587568356 1102 7 0.38806444675333296 1102 8 0.394365112106442 1102 9 0.557864520134693 1102 10-11 0.4345336536979687 1102 12-13 0.5238010807650895 1102 14-15 0.6058713156435758 1102 16-17 0.5862240961294205 1102 18-19 0.6173540229599723 1102 20-21 0.5315365124341653 1102 22-23 0.4794831774293655 1102 24-25 0.5534964479572579 1102 26-27 0.5037409045920072 1102 28-29 0.5410660919716932 1102 30-31 0.49410355130820705 1102 32-33 0.5516251681351605 1102 34-35 0.6316820183764733 1102 36-37 0.5562717896665177 1102 38-39 0.5687507449289058 1102 40-41 0.6440526907371265 1102 42-43 0.5812633518847647 1102 44-45 0.5776111130137238 1102 46-47 0.5038053213924556 1102 48-49 0.5158995017264161 1102 50-51 0.622875873426473 1102 52-53 0.5699123824622916 1102 54-55 0.5904252481225214 1102 56-57 0.566415412904334 1102 58-59 0.6911924352256236 1102 60-61 0.6020064791835082 1102 62-63 0.5964001684452853 1102 64-65 0.5662456631684663 1102 66-67 0.5888343772985323 1102 68-69 0.6606845828136869 1102 70-71 0.6798321189006344 1102 72-73 0.6642075808241259 1102 74-75 0.6110752317247687 1102 76-77 0.6374837754496951 1102 78-79 0.5986942476233423 1102 80-81 0.5783756665184399 1102 82-83 0.5650542063494228 1102 84-85 0.620356315187756 1102 86-87 0.5923903096870546 1102 88-89 0.6520458734966219 1102 90-91 0.7055992317394164 1102 92-93 0.681696493653277 1102 94-95 0.675039928294666 1102 96-97 0.6449715265022142 1102 98-99 0.6929644302712745 1102 100 0.6608672598052507 1103 1 0.12122494787099569 1103 2 0.20610781374278275 1103 3 0.2190514749206045 1103 4 0.2641786858265647 1103 5 0.2581088446741404 1103 6 0.3471427600248731 1103 7 0.34737484925104667 1103 8 0.35497785419892836 1103 9 0.4233032831993455 1103 10-11 0.4729913110292614 1103 12-13 0.4249717328579763 1103 14-15 0.5441192865811573 1103 16-17 0.5138272879107362 1103 18-19 0.5276123254017691 1103 20-21 0.490695314329038 1103 22-23 0.48707751722341186 1103 24-25 0.4827319936659933 1103 26-27 0.4999710907501509 1103 28-29 0.4852269164622314 1103 30-31 0.48220002807837403 1103 32-33 0.5500259919582717 1103 34-35 0.47095742662568796 1103 36-37 0.44363264926569457 1103 38-39 0.5122798160243534 1103 40-41 0.4690232312383742 1103 42-43 0.4659147095245473 1103 44-45 0.40991774225727085 1103 46-47 0.4802689649532397 1103 48-49 0.4354712051347036 1103 50-51 0.39636566167824583 1103 52-53 0.4501707199525171 1103 54-55 0.4297948140966952 1103 56-57 0.4016583268259595 1103 58-59 0.4860670898776007 1103 60-61 0.4361635797242016 1103 62-63 0.4558659365647202 1103 64-65 0.46332694786170947 1103 66-67 0.4960208780351607 1103 68-69 0.5237464707575654 1103 70-71 0.3799914882145998 1103 72-73 0.4064702143922574 1103 74-75 0.4143749923138671 1103 76-77 0.5161477747370284 1103 78-79 0.40707726748713924 1103 80-81 0.3960966105013455 1103 82-83 0.4080059910360987 1103 84-85 0.4723104621173775 1103 86-87 0.4077064473126697 1103 88-89 0.42836600554707616 1103 90-91 0.4622835109966523 1103 92-93 0.4182946552532627 1103 94-95 0.41895221423501283 1103 96-97 0.3824076036957216 1103 98-99 0.37310430620540913 1103 100 0.3050041911548931 1104 1 0.05630635350457425 1104 2 0.132110350210354 1104 3 0.12910281264985812 1104 4 0.26053777850123083 1104 5 0.24591086916836957 1104 6 0.33404859717617086 1104 7 0.3174707389568283 1104 8 0.27450350668414814 1104 9 0.41958047885833594 1104 10-11 0.3720275001505229 1104 12-13 0.3597711787661595 1104 14-15 0.4668377527202523 1104 16-17 0.4378291972811539 1104 18-19 0.4532322858104152 1104 20-21 0.43891066136179546 1104 22-23 0.3869818147108077 1104 24-25 0.4644970381477691 1104 26-27 0.4067465478524923 1104 28-29 0.40026461248859135 1104 30-31 0.38299115324976896 1104 32-33 0.38981670998450824 1104 34-35 0.41693113534373794 1104 36-37 0.3715585956442098 1104 38-39 0.45716897426342484 1104 40-41 0.4785455142463846 1104 42-43 0.40609946919190776 1104 44-45 0.3493162905741585 1104 46-47 0.34580459483488823 1104 48-49 0.3030554303655322 1104 50-51 0.4193377745744442 1104 52-53 0.41240332619634046 1104 54-55 0.4270149953932787 1104 56-57 0.41768557279311835 1104 58-59 0.4691600359706456 1104 60-61 0.5096544517168269 1104 62-63 0.45591741468885516 1104 64-65 0.47901373623513877 1104 66-67 0.5152304080398906 1104 68-69 0.5108371611728089 1104 70-71 0.5029914460961322 1104 72-73 0.5168053837786886 1104 74-75 0.5044500099802178 1104 76-77 0.5914052823534561 1104 78-79 0.5075602258881347 1104 80-81 0.548522166114104 1104 82-83 0.45203499661913327 1104 84-85 0.4249178291049205 1104 86-87 0.4463548189660038 1104 88-89 0.4371769544760973 1104 90-91 0.4120701107729552 1104 92-93 0.4861861112886068 1104 94-95 0.5338502173517767 1104 96-97 0.4956758562006023 1104 98-99 0.4544549517878842 1104 100 0.44449820799292183 1105 1 0.05921713458758404 1105 2 0.06061761002928279 1105 3 0.08746978804082417 1105 4 0.15240328809328219 1105 5 0.1890594046725056 1105 6 0.22669201242218406 1105 7 0.2072277527278672 1105 8 0.23429552821122712 1105 9 0.3376973619050361 1105 10-11 0.34038273890195114 1105 12-13 0.23934791474337658 1105 14-15 0.2809930715600899 1105 16-17 0.3385960660583791 1105 18-19 0.2537521379170613 1105 20-21 0.3008496816770503 1105 22-23 0.2837984563993956 1105 24-25 0.23487864066304098 1105 26-27 0.23833367674852468 1105 28-29 0.3308827100120695 1105 30-31 0.25706540368791053 1105 32-33 0.20299024919862774 1105 34-35 0.30117653623476315 1105 36-37 0.3009646583523278 1105 38-39 0.281979903197346 1105 40-41 0.3317498794532341 1105 42-43 0.2855981724089318 1105 44-45 0.23592663354080656 1105 46-47 0.2005589666810721 1105 48-49 0.23559757536190773 1105 50-51 0.26385727471802056 1105 52-53 0.24074123180706408 1105 54-55 0.29799318573221 1105 56-57 0.26912814102189486 1105 58-59 0.323728037923253 1105 60-61 0.23222072732096422 1105 62-63 0.2163388264218824 1105 64-65 0.23037682253819725 1105 66-67 0.25347711510143967 1105 68-69 0.26661495813934977 1105 70-71 0.2748765578538226 1105 72-73 0.2664775000683761 1105 74-75 0.2683115085062511 1105 76-77 0.3060275287337717 1105 78-79 0.17740586934225178 1105 80-81 0.23376740515496408 1105 82-83 0.2074857605595568 1105 84-85 0.1919015988112207 1105 86-87 0.20711136023701826 1105 88-89 0.16713729698036772 1105 90-91 0.24662567712386263 1105 92-93 0.248797414307397 1105 94-95 0.1376230897976285 1105 96-97 0.15142454175341769 1105 98-99 0.1922751758376826 1105 100 0.1306219035587901 1106 1 0.07535013976642446 1106 2 0.06722317038141767 1106 3 0.16848892370847324 1106 4 0.17410645681678005 1106 5 0.2079168152912061 1106 6 0.24131927905063577 1106 7 0.27340203129858764 1106 8 0.26779889013347713 1106 9 0.33384552395159517 1106 10-11 0.2585620384904814 1106 12-13 0.303760283177418 1106 14-15 0.3678644585502937 1106 16-17 0.37955104644777293 1106 18-19 0.3643271363168452 1106 20-21 0.32417374596523274 1106 22-23 0.3839782866248811 1106 24-25 0.4191500433866864 1106 26-27 0.311101202548663 1106 28-29 0.3896919724120451 1106 30-31 0.3374228946839466 1106 32-33 0.3678383360471855 1106 34-35 0.3992164239117244 1106 36-37 0.3552843021317287 1106 38-39 0.3448504439046829 1106 40-41 0.3655976179185956 1106 42-43 0.3560062986790271 1106 44-45 0.2741404880514935 1106 46-47 0.30431980479428944 1106 48-49 0.3639183243906672 1106 50-51 0.3286499818313473 1106 52-53 0.2973475104247001 1106 54-55 0.2780491811235848 1106 56-57 0.22076029918493134 1106 58-59 0.29479931380219426 1106 60-61 0.32788363506489304 1106 62-63 0.3137342784820589 1106 64-65 0.40847543208369785 1106 66-67 0.4192302946477824 1106 68-69 0.38724334760733115 1106 70-71 0.37916577551611397 1106 72-73 0.3965183923397859 1106 74-75 0.39504656186556275 1106 76-77 0.48185115204602624 1106 78-79 0.362099162648164 1106 80-81 0.43674099497259533 1106 82-83 0.3920006308539996 1106 84-85 0.3768810940415932 1106 86-87 0.36263510037735713 1106 88-89 0.42497199526244245 1106 90-91 0.40994937006389875 1106 92-93 0.4209737049987261 1106 94-95 0.4204076927262932 1106 96-97 0.4170777406978097 1106 98-99 0.4296370306630344 1106 100 0.3970472525649704 1107 1 0.0957299620974652 1107 2 0.07083990960791198 1107 3 0.1356214703532146 1107 4 0.18067929070668498 1107 5 0.18898809294784513 1107 6 0.2531515777122024 1107 7 0.21930876354677764 1107 8 0.23211722615913288 1107 9 0.275347765941639 1107 10-11 0.2794889691735136 1107 12-13 0.30198678091610276 1107 14-15 0.3154647685896279 1107 16-17 0.33733469984118614 1107 18-19 0.2930753645772697 1107 20-21 0.32401925751806715 1107 22-23 0.2940938752770563 1107 24-25 0.3293043145165626 1107 26-27 0.3642739142579643 1107 28-29 0.39505565289767475 1107 30-31 0.4474561925221181 1107 32-33 0.42567008900088865 1107 34-35 0.39208764410001606 1107 36-37 0.44063721333829875 1107 38-39 0.502417359520372 1107 40-41 0.48729713571567146 1107 42-43 0.46733123390639975 1107 44-45 0.4006575921295692 1107 46-47 0.40617434225858773 1107 48-49 0.4539712773704494 1107 50-51 0.5436927329716568 1107 52-53 0.5356653297181282 1107 54-55 0.5472773425468631 1107 56-57 0.5111387934658609 1107 58-59 0.5390391258468767 1107 60-61 0.5280124317790609 1107 62-63 0.5240444868410492 1107 64-65 0.5712324641090234 1107 66-67 0.4686772704362241 1107 68-69 0.48180027387157764 1107 70-71 0.5326700168252358 1107 72-73 0.5399533577259703 1107 74-75 0.6515132241858836 1107 76-77 0.7108120614947424 1107 78-79 0.6025638353079614 1107 80-81 0.5587349052591861 1107 82-83 0.5635404204425711 1107 84-85 0.5405286440306085 1107 86-87 0.6205497719505715 1107 88-89 0.6076053013103824 1107 90-91 0.601338679501989 1107 92-93 0.5667985810675056 1107 94-95 0.5663646011222276 1107 96-97 0.5223348391256444 1107 98-99 0.513210064967776 1107 100 0.4987267288785908 1108 1 -0.02771038848275964 1108 2 0.02380266841618095 1108 3 0.11785779371688676 1108 4 0.0034531164032713946 1108 5 0.05121637229512288 1108 6 0.1374699413625109 1108 7 0.11229267406722698 1108 8 0.0956192378866163 1108 9 0.206077210658556 1108 10-11 0.1292818646866607 1108 12-13 0.1610742769555813 1108 14-15 0.2425490875319909 1108 16-17 0.24327439576000387 1108 18-19 0.20430342256941714 1108 20-21 0.2798934872962562 1108 22-23 0.23223799337942097 1108 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-0.6651816412170177 2215 48-49 -0.5792178474634824 2215 50-51 -0.6115552292936215 2215 52-53 -0.578877513431749 2215 54-55 -0.7145119681537722 2215 56-57 -0.6110986479872125 2215 58-59 -0.5374068131908771 2215 60-61 -0.4575939965477289 2215 62-63 -0.5102974606436064 2215 64-65 -0.468314633032513 2215 66-67 -0.5242058651410346 2215 68-69 -0.5511486640485046 2215 70-71 -0.5272207592119216 2215 72-73 -0.5784354029371883 2215 74-75 -0.5881816355378362 2215 76-77 -0.6239811545156826 2215 78-79 -0.5694894937495718 2215 80-81 -0.534446891437824 2215 82-83 -0.5555313331255078 2215 84-85 -0.5562938605306158 2215 86-87 -0.4627924784037525 2215 88-89 -0.47227522696993773 2215 90-91 -0.49957854654640244 2215 92-93 -0.48084853432619923 2215 94-95 -0.5410593415063332 2215 96-97 -0.4155099614421367 2215 98-99 -0.5793501490116384 2215 100 -0.5103661055815039 2216 1 -0.9383022613748437 2216 2 -0.9081970913202966 2216 3 -1.049331612698019 2216 4 -1.328295754908872 2216 5 -1.243646995412739 2216 6 -2.6627128662277784 2216 7 -2.44175067128576 2216 8 -3.2976235819963584 2216 9 -2.762833208161666 2216 10-11 -1.9742887233194324 2216 12-13 -1.7473959931640977 2216 14-15 -2.0084819122928153 2216 16-17 -2.4474714419938195 2216 18-19 -2.0183834880572675 2216 20-21 -1.8631232155533937 2216 22-23 -2.043636800450699 2216 24-25 -2.213744973325916 2216 26-27 -2.281768382655457 2216 28-29 -2.7053191978961095 2216 30-31 -2.312868886462361 2216 32-33 -2.5222797063754214 2216 34-35 -3.468336928733123 2216 36-37 -2.478169243890818 2216 38-39 -2.410081123186913 2216 40-41 -2.9902564345097957 2216 42-43 -2.7257996720719646 2216 44-45 -2.543500183455258 2216 46-47 -2.398862507332055 2216 48-49 -2.5617638568245837 2216 50-51 -2.91190226374583 2216 52-53 -3.181521704984533 2216 54-55 -2.6207886712300095 2216 56-57 -2.56887992004134 2216 58-59 -3.6583013515042353 2216 60-61 -4.347752525685593 2216 62-63 -3.4566556512337456 2216 64-65 -3.308812627480158 2216 66-67 -3.040679879484834 2216 68-69 -3.3736074679572354 2216 70-71 -3.1143593383301997 2216 72-73 -3.556707280323149 2216 74-75 -4.077250295387586 2216 76-77 -4.746477685514378 2216 78-79 -3.6874828996117657 2216 80-81 -3.525182114566867 2216 82-83 -3.7622123756387253 2216 84-85 -3.8009395165092386 2216 86-87 -3.4996541061067497 2216 88-89 -3.517225902467491 2216 90-91 -3.6787306058206575 2216 92-93 -3.758373583843987 2216 94-95 -3.6042125747216076 2216 96-97 -3.8742378996335596 2216 98-99 -3.6372304775727784 2216 100 -3.284890528285679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70411.0 3 12495.0 4 8052.0 5 6553.0 6 5901.0 7 6111.0 8 6816.0 9 7791.0 10 8918.0 11 10021.0 12 11282.0 13 12678.0 14 14451.0 15 15499.0 16 15834.0 17 16816.0 18 15841.0 19 14202.0 20 14321.0 21 14647.0 22 16501.0 23 19463.0 24 23938.0 25 29327.0 26 35582.0 27 42555.0 28 52224.0 29 64548.0 30 78138.0 31 93713.0 32 119146.0 33 157516.0 34 216630.0 35 311815.0 36 507666.0 37 896921.0 38 1210509.0 39 447958.0 40 1429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37722832777089 20.336408373815214 14.252757359515723 23.033605938898173 2 15.091933481209718 20.12004134063508 40.97431907087204 23.813706107283153 3 18.101236073253794 25.417574736338196 30.42352832006605 26.05766087034196 4 12.369724053163884 16.843786377476054 39.37896405403982 31.407525515320252 5 12.908612795821744 38.109715641660095 35.843530494346574 13.138141068171588 6 30.745480997096823 38.157166118481676 17.80579290965897 13.291559974762531 7 26.738701712727643 32.77194939088852 22.767357313890795 17.721991582493043 8 24.518073334027125 36.85396936899434 20.70608717521768 17.92187012176085 9 24.51513407826801 17.157361198498105 21.975428343219207 36.35207638001468 10-11 23.555267291310667 27.305201841041544 29.23285997024682 19.90667089740097 12-13 23.877838941130328 24.76388365824061 29.393760817685294 21.964516582943773 14-15 21.305863568211176 27.049492126466955 26.480548452898805 25.164095852423056 16-17 20.86457968042086 30.47142286485177 27.154394958452325 21.509602496275047 18-19 20.80312449049679 29.4732105380007 29.135984455599072 20.58768051590344 20-21 21.892056080642483 28.601617654136994 28.758858267289746 20.747467997930777 22-23 21.505197183361208 28.42874041748314 28.838448706568904 21.227613692586743 24-25 21.41290583164389 28.557348736783933 28.750303795816258 21.279441635755916 26-27 21.156770918135166 28.582750366452323 28.823434910890455 21.437043804522045 28-29 21.13520298931042 28.60818634842551 28.919843856878202 21.336766805385878 30-31 21.411227768093198 28.92693992895936 28.550576534432338 21.111255768515104 32-33 21.162610270878908 28.685545215301588 28.890425551634884 21.26141896218462 34-35 21.33829800634391 28.435049400476604 28.91268002477205 21.313972568407436 36-37 21.510632900964104 28.789525145417848 28.44435965199444 21.25548230162361 38-39 21.362301122687384 28.891186726817075 28.549943702488985 21.196568448006563 40-41 21.324866333202927 28.64065666839133 28.712548337445533 21.32192866096021 42-43 21.232321267963556 28.635890071292163 28.68343480220102 21.44835385854326 44-45 21.347712196541725 28.738243371491844 28.572478500268016 21.341565931698412 46-47 21.360357698245437 28.817882223985023 28.438855457536665 21.38290462023287 48-49 21.301950702793267 28.694472134334823 28.467192213318583 21.53638494955333 50-51 21.378822617828245 28.645453649673136 28.389628727577787 21.586095004920836 52-53 21.344951869527996 28.61997506999707 28.49792667318444 21.53714638729049 54-55 21.47510981720204 28.887612420154007 28.280096168031942 21.35718159461201 56-57 21.35834422462411 28.693399568857725 28.37079623004704 21.577459976471125 58-59 21.30557886133387 28.408334579921473 28.62658506392287 21.659501494821782 60-61 21.48974296464619 28.730690587735 28.295308441665785 21.484258005953023 62-63 21.336225842953986 28.747257378614922 28.364469220040267 21.55204755839083 64-65 21.397289267535978 28.510070536190163 28.451504458793835 21.641135737480024 66-67 21.259425491478346 28.58908483020918 28.359728449155654 21.791761229156823 68-69 21.560890192359327 28.716537490920334 28.28579750418605 21.43677481253429 70-71 21.52023598907034 28.582987007398124 28.35148999373779 21.545287009793746 72-73 21.40739630285665 28.693731588290518 28.403089844860634 21.495782263992194 74-75 21.52145602815523 28.712451869431288 28.283951331895135 21.48214077051835 76-77 21.52293204259623 28.673196591599016 28.178157014122974 21.62571435168178 78-79 21.552822224725006 28.740051941981683 28.232295711366568 21.47483012192675 80-81 21.519651584683533 28.618132373192363 28.319367410012802 21.542848632111305 82-83 21.405236108547246 28.72512625144451 28.415926873507047 21.453710766501192 84-85 21.489895592906098 28.579484031521467 28.293217880203976 21.63740249536846 86-87 21.629155569430488 28.743028659323794 28.18172259456905 21.446093176676673 88-89 21.46672845377755 28.605524526502062 28.260633209488116 21.667113810232273 90-91 21.453797422662195 28.537140421572353 28.33341535523759 21.675646800527858 92-93 21.480893166190768 28.751477723634622 28.173320045292648 21.59430906488196 94-95 21.449294119968062 28.551540548292497 28.260588200479077 21.738577131260364 96-97 21.496259713724033 28.585921041025582 28.223259450840978 21.69455979440941 98-99 21.494753499996424 28.6201305139613 28.169761773335857 21.71535421270642 100 21.573249990951883 28.516548520995645 28.166370083431236 21.743831404621236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1461.0 1 1214.5 2 1149.0 3 1331.0 4 1251.5 5 1024.0 6 795.0 7 712.0 8 698.5 9 688.0 10 673.0 11 631.5 12 628.5 13 730.5 14 854.0 15 967.5 16 1226.5 17 1640.0 18 2215.0 19 3037.5 20 4382.5 21 6550.0 22 9329.0 23 13338.5 24 19118.5 25 26455.0 26 36225.5 27 48163.5 28 61865.0 29 77319.5 30 92561.0 31 105964.5 32 117009.5 33 126828.0 34 137318.0 35 146092.5 36 152630.5 37 159544.5 38 165566.0 39 168895.0 40 170121.0 41 169422.0 42 167684.0 43 165304.5 44 161373.5 45 157121.0 46 152402.0 47 146113.5 48 140220.0 49 136392.0 50 132586.5 51 127350.5 52 119688.0 53 112102.5 54 106557.5 55 101387.0 56 93974.5 57 84935.5 58 76948.5 59 69911.0 60 61741.0 61 52435.0 62 44497.5 63 37117.0 64 29757.0 65 23627.5 66 18694.5 67 14211.0 68 11005.5 69 8603.0 70 6400.5 71 4888.0 72 3618.5 73 2501.0 74 1807.0 75 1254.0 76 843.5 77 584.5 78 419.5 79 320.0 80 227.5 81 134.0 82 97.0 83 81.0 84 62.5 85 47.5 86 33.0 87 29.0 88 28.0 89 27.0 90 24.0 91 19.5 92 12.0 93 9.5 94 10.0 95 9.5 96 10.5 97 10.0 98 8.0 99 16.5 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018161253291185356 2 0.03918322905783189 3 0.040548573875665635 4 0.043127558531573816 5 0.04661677306603783 6 0.0439727719902328 7 0.04176221371374007 8 0.04115539379470285 9 0.041458803754221465 10-11 0.042011443323344645 12-13 0.042910837131917665 14-15 0.041166229864685655 16-17 0.03785039244994657 18-19 0.04080863955525301 20-21 0.04033185247600948 22-23 0.041458803754221465 24-25 0.03783955637996376 26-27 0.03792624493982622 28-29 0.038663097698657126 30-31 0.038760622328502393 32-33 0.03837052380912133 34-35 0.038218818829362025 36-37 0.03573735880329911 38-39 0.03620330981255983 40-41 0.03735193323073742 42-43 0.03728691681084058 44-45 0.03600826055286929 46-47 0.035022178184433816 48-49 0.035217227444124345 50-51 0.036615080471906515 52-53 0.03694016257139074 54-55 0.03613829339266298 56-57 0.0344261943353794 58-59 0.03530391600398681 60-61 0.03477294857482924 62-63 0.035011342114451005 64-65 0.032085603219092984 66-67 0.0331258659374425 68-69 0.034144456515826406 70-71 0.03537976849386646 72-73 0.03241068531857721 74-75 0.032638242788216164 76-77 0.033754357996445335 78-79 0.03407944009592956 80-81 0.03387355476625622 82-83 0.032497373878439664 84-85 0.03366766943658288 86-87 0.03432866970553414 88-89 0.03481629285476047 90-91 0.03532558814395242 92-93 0.033591816946703226 94-95 0.0348488010647089 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4614219.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.34525984141536 #Duplication Level Percentage of deduplicated Percentage of total 1 78.26816367130262 51.92721656082998 2 13.483531050125471 17.891367422007328 3 4.012918647236565 7.9871439112011275 4 1.5951589256445655 4.233249336409667 5 0.8147631491259916 2.702783641898688 6 0.45483619008265774 1.810573512978799 7 0.2896874460923239 1.3453572218653862 8 0.2090784561559251 1.1097091600725455 9 0.1481100055335506 0.8843757122013194 >10 0.6852235541670103 7.8297753247481605 >50 0.029962102559138944 1.3410601605598422 >100 0.008435989803412158 0.8496023424635939 >500 6.540615009447837E-5 0.030744550115701357 >1k 6.540602061821158E-5 0.05704114264786583 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.1672139965614984E-5 0.0 2 0.0 0.0 0.0 2.1672139965614984E-5 0.0 3 0.0 0.0 0.0 4.334427993122997E-5 0.0 4 0.0 0.0 0.0 8.668855986245993E-5 0.0 5 0.0 0.0 0.0 8.668855986245993E-5 0.0 6 0.0 0.0 0.0 8.668855986245993E-5 0.0 7 0.0 0.0 0.0 8.668855986245993E-5 0.0 8 0.0 0.0 0.0 8.668855986245993E-5 0.0 9 0.0 0.0 0.0 8.668855986245993E-5 0.0 10-11 0.0 0.0 0.0 1.300328397936899E-4 0.0 12-13 0.0 0.0 0.0 1.517049797593049E-4 0.0 14-15 0.0 0.0 0.0 1.6254104974211238E-4 0.0 16-17 0.0 0.0 0.0 1.7337711972491987E-4 0.0 18-19 0.0 0.0 0.0 2.1672139965614982E-4 0.0 20-21 0.0 0.0 0.0 3.1424602950141727E-4 0.0 22-23 0.0 0.0 0.0 3.684263794154547E-4 0.0 24-25 0.0 0.0 0.0 5.092952891919521E-4 0.0 26-27 0.0 0.0 0.0 9.210659485386367E-4 0.0 28-29 0.0 0.0 0.0 0.0024706239560801082 0.0 30-31 0.0 0.0 0.0 0.007650265407862089 0.0 32-33 0.0 0.0 0.0 0.017890351541615168 0.0 34-35 0.0 0.0 0.0 0.03388439083623902 0.0 36-37 0.0 0.0 0.0 0.05891571249652433 0.0 38-39 0.0 0.0 0.0 0.0924316769533479 0.0 40-41 0.0 0.0 0.0 0.12590429713024023 0.0 42-43 0.0 0.0 0.0 0.15875926131811255 0.0 44-45 0.0 0.0 0.0 0.20129083600063197 0.0 46-47 0.0 0.0 0.0 0.2528163487688816 0.0 48-49 0.0 0.0 0.0 0.3048294846863575 0.0 50-51 0.0 0.0 0.0 0.35602991535512296 0.0 52-53 0.0 0.0 0.0 0.40654767361497146 0.0 54-55 0.0 0.0 0.0 0.46292774573551887 0.0 56-57 0.0 0.0 0.0 0.5275865753229312 0.0 58-59 0.0 0.0 0.0 0.5982269155408532 0.0 60-61 0.0 0.0 0.0 0.6724106506431533 0.0 62-63 0.0 0.0 0.0 0.7444596799588403 0.0 64-65 0.0 0.0 0.0 0.8231512201739883 0.0 66-67 0.0 0.0 0.0 0.9086369762683566 0.0 68-69 0.0 0.0 0.0 1.005424753354793 0.0 70-71 0.0 0.0 0.0 1.106081007425092 0.0 72-73 0.0 0.0 0.0 1.2082651473629666 0.0 74-75 0.0 0.0 0.0 1.3116087467890016 0.0 76-77 0.0 0.0 0.0 1.424672300989615 0.0 78-79 0.0 0.0 0.0 1.5518985986577576 0.0 80-81 0.0 0.0 0.0 1.6839469474682498 0.0 82-83 0.0 0.0 0.0 1.8211641016605409 0.0 84-85 0.0 0.0 0.0 1.9598983056504253 0.0 86-87 0.0 0.0 0.0 2.1109097769308303 0.0 88 0.0 0.0 0.0 2.2307350388007157 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4415 0.0 44.827106 1 GTATCAA 7530 0.0 37.270798 1 TCAACGC 8125 0.0 33.324234 4 ATCAACG 8270 0.0 32.739952 3 CAACGCA 8655 0.0 31.283922 5 AACGCAG 9155 0.0 29.623486 6 TATCAAC 9355 0.0 29.093493 2 GTACATG 12310 0.0 25.77716 1 ACGCAGA 10785 0.0 24.840977 7 CGCAGAG 10855 0.0 24.50759 8 TACATGG 12605 0.0 23.792437 2 ACATGGG 12775 0.0 22.408743 3 GCAGAGT 12815 0.0 19.69562 9 CATGGGG 9320 0.0 19.266754 4 GTATAGG 1555 0.0 18.743498 1 GAGTACA 9255 0.0 18.18427 1 AGAGTAC 11040 0.0 17.689514 10-11 TATACTG 3035 0.0 17.037262 5 CAGAGTA 12415 0.0 16.94183 10-11 GTGGTAT 2905 0.0 16.829706 1 >>END_MODULE