FastQCFastQC Report
Wed 25 May 2016
SRR2049383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614219
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105320.228250978117857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97310.21089159400539942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57190.1239429684633521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66300.057.154211
GTATCAA125300.041.911081
TCAACGC152650.033.7423824
ATCAACG153450.033.627723
CAACGCA158100.032.6089445
TATCAAC162150.032.0553252
AACGCAG164950.031.3059886
ACGCAGA189050.027.0663437
CGCAGAG191300.026.6004928
GTGGTAT31250.024.5224741
GCAGAGT211850.023.6869249
GTACATG115350.023.3133741
TGGTATC30950.021.865722
TACATGG119050.021.5538652
GAGTACT148650.021.26876612-13
AGAGTAC194600.020.89271410-11
CAGAGTA208850.020.50303610-11
ACATGGG121500.020.4616833
AGTACTT162400.018.67190412-13
GTACTTT170550.018.42073414-15
Produced by FastQC (version 0.11.5)