##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049383_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4614219 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90390984909906 33.0 31.0 34.0 30.0 34.0 2 32.085549688907264 33.0 31.0 34.0 30.0 34.0 3 32.01421215594665 34.0 31.0 34.0 30.0 34.0 4 35.740896346705696 37.0 35.0 37.0 33.0 37.0 5 35.80561152385702 37.0 35.0 37.0 35.0 37.0 6 35.83484138919284 37.0 35.0 37.0 35.0 37.0 7 35.81083364270313 37.0 35.0 37.0 35.0 37.0 8 35.808472896496674 37.0 35.0 37.0 35.0 37.0 9 37.55017653041609 39.0 37.0 39.0 35.0 39.0 10-11 37.51104217203388 39.0 37.0 39.0 35.0 39.0 12-13 37.45577268872587 39.0 37.0 39.0 34.5 39.0 14-15 38.76583144406453 40.0 38.0 41.0 35.0 41.0 16-17 38.79650315687226 40.0 38.0 41.0 35.0 41.0 18-19 38.862966842276016 40.0 38.0 41.0 35.0 41.0 20-21 38.89176207284483 40.0 38.0 41.0 35.0 41.0 22-23 38.86363661976165 40.0 38.0 41.0 35.0 41.0 24-25 38.84641723767338 40.0 38.0 41.0 35.0 41.0 26-27 38.73652464263183 40.0 38.0 41.0 34.5 41.0 28-29 38.663214403130844 40.0 38.0 41.0 34.0 41.0 30-31 38.57120273225003 40.0 38.0 41.0 34.0 41.0 32-33 38.44218306499973 40.0 38.0 41.0 34.0 41.0 34-35 38.34669810860733 40.0 38.0 41.0 34.0 41.0 36-37 38.243164877956595 40.0 38.0 41.0 34.0 41.0 38-39 38.15697055124605 40.0 38.0 41.0 34.0 41.0 40-41 38.05205561331181 40.0 38.0 41.0 33.0 41.0 42-43 37.92432923101396 40.0 38.0 41.0 33.0 41.0 44-45 37.76231340558391 40.0 37.0 41.0 33.0 41.0 46-47 37.54970071858314 40.0 37.0 41.0 33.0 41.0 48-49 37.3604602425676 40.0 37.0 41.0 32.0 41.0 50-51 37.22840084096572 40.0 37.0 41.0 32.0 41.0 52-53 37.095377245856774 40.0 37.0 41.0 31.5 41.0 54-55 36.963907868265466 40.0 36.0 41.0 31.0 41.0 56-57 36.82935053581116 39.5 36.0 41.0 31.0 41.0 58-59 36.64732482355086 39.0 35.5 41.0 31.0 41.0 60-61 36.47834703987826 39.0 35.0 41.0 31.0 41.0 62-63 36.281903394702326 39.0 35.0 41.0 31.0 41.0 64-65 36.06024486484061 38.5 35.0 40.5 30.5 41.0 66-67 35.80273931514738 38.0 35.0 40.0 30.0 41.0 68-69 35.52427962348558 37.0 35.0 40.0 30.0 41.0 70-71 35.19717952268846 37.0 35.0 40.0 29.0 41.0 72-73 34.904246525793425 36.5 34.0 39.0 29.0 41.0 74-75 34.59439734004823 36.0 34.0 39.0 29.0 41.0 76-77 34.266799430196095 35.5 34.0 39.0 29.0 40.0 78-79 33.92774454355114 35.0 34.0 37.5 29.0 39.5 80-81 33.606727812442365 35.0 34.0 37.0 29.0 39.0 82-83 33.29956152926422 35.0 34.0 36.5 29.0 39.0 84-85 33.020792142722314 35.0 34.0 36.0 29.0 37.5 86-87 32.78059819007291 35.0 34.0 36.0 28.5 37.0 88-89 32.55032628490325 35.0 33.0 35.5 27.5 37.0 90-91 32.350905429499555 35.0 33.0 35.0 27.0 36.0 92-93 32.16517573179773 35.0 33.0 35.0 27.0 36.0 94-95 32.00937309217443 35.0 33.0 35.0 27.0 36.0 96-97 31.81491060567346 35.0 33.0 35.0 26.0 36.0 98-99 31.668070479532936 35.0 33.0 35.0 26.0 35.5 100 31.553185056886118 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13679483347040033 1101 2 0.17215784175805737 1101 3 0.3257738186215846 1101 4 0.19725696144725902 1101 5 0.15046584573246236 1101 6 0.15118407084728602 1101 7 0.05652523911670215 1101 8 0.10234985873341174 1101 9 0.22619244721160214 1101 10-11 0.28438854593524354 1101 12-13 0.2338863232626167 1101 14-15 0.32798019675990275 1101 16-17 0.2500549048273797 1101 18-19 0.29396835216611095 1101 20-21 0.34770209608420544 1101 22-23 0.2941773718979235 1101 24-25 0.2949909318817632 1101 26-27 0.3635539956159164 1101 28-29 0.3294623299792505 1101 30-31 0.37153533248493886 1101 32-33 0.40305034022857456 1101 34-35 0.37858453546514426 1101 36-37 0.45833095325860285 1101 38-39 0.40415948292697834 1101 40-41 0.3982153236493957 1101 42-43 0.4096405498775937 1101 44-45 0.4579584936773742 1101 46-47 0.43758515764169204 1101 48-49 0.5137211176684886 1101 50-51 0.481292510631917 1101 52-53 0.4943746972200671 1101 54-55 0.5039200473565302 1101 56-57 0.5303395061837577 1101 58-59 0.5690922956499662 1101 60-61 0.4994152536439316 1101 62-63 0.5072663603660104 1101 64-65 0.44544956766531385 1101 66-67 0.4708091668144334 1101 68-69 0.42397911816682665 1101 70-71 0.4409794007308392 1101 72-73 0.34001991470763215 1101 74-75 0.39827371775187004 1101 76-77 0.34508176560976267 1101 78-79 0.404524988430893 1101 80-81 0.4527536521201583 1101 82-83 0.4022635394618348 1101 84-85 0.4358349593595108 1101 86-87 0.31308011429700144 1101 88-89 0.39031904895284697 1101 90-91 0.4027196861264102 1101 92-93 0.3974882016183585 1101 94-95 0.425457277472411 1101 96-97 0.4345430771837506 1101 98-99 0.47437588139840514 1101 100 0.4873657936016116 1102 1 0.2550915667891367 1102 2 0.22665031934344881 1102 3 0.37303002314808964 1102 4 0.19348958224721713 1102 5 0.13593764193154811 1102 6 0.12322880493584165 1102 7 0.11270321982233611 1102 8 0.185500822456369 1102 9 0.17613090521061991 1102 10-11 0.1953550893851883 1102 12-13 0.16789311234965254 1102 14-15 0.2293414429964784 1102 16-17 0.19055465153624596 1102 18-19 0.27395493547897587 1102 20-21 0.21292105464843303 1102 22-23 0.2440143585962673 1102 24-25 0.28716352821535196 1102 26-27 0.32186610337414834 1102 28-29 0.3213721893086543 1102 30-31 0.21410253205843333 1102 32-33 0.251531555760522 1102 34-35 0.292104341946299 1102 36-37 0.3237035013673264 1102 38-39 0.34231073422937897 1102 40-41 0.338951726119042 1102 42-43 0.3882089130417583 1102 44-45 0.38709564473843017 1102 46-47 0.3008925535213436 1102 48-49 0.26392118447540724 1102 50-51 0.33094870940682597 1102 52-53 0.4131318562891053 1102 54-55 0.3320491992847536 1102 56-57 0.39140308557448833 1102 58-59 0.36036360694586733 1102 60-61 0.32432014227326533 1102 62-63 0.3320636057973658 1102 64-65 0.24021559966626427 1102 66-67 0.26379758178713786 1102 68-69 0.394112610332499 1102 70-71 0.362587854681621 1102 72-73 0.334560053055462 1102 74-75 0.28550236995550193 1102 76-77 0.29206670529551104 1102 78-79 0.35048158826852216 1102 80-81 0.3482840149790505 1102 82-83 0.367796767054422 1102 84-85 0.3105108241440888 1102 86-87 0.362656941145417 1102 88-89 0.3228945551479683 1102 90-91 0.35450481340243556 1102 92-93 0.387774398468693 1102 94-95 0.31227416015548215 1102 96-97 0.4041144068404954 1102 98-99 0.49492060110799585 1102 100 0.4818388927567909 1103 1 0.1294533600742902 1103 2 0.12418602955835212 1103 3 0.3097620740656808 1103 4 0.15048496043390713 1103 5 0.1409087999733032 1103 6 0.13748674433607277 1103 7 0.1365735684839393 1103 8 0.13811162007034739 1103 9 0.1688919844882193 1103 10-11 0.16153253367102138 1103 12-13 0.15177266047255245 1103 14-15 0.20893242820387314 1103 16-17 0.13558322579326898 1103 18-19 0.256912903311445 1103 20-21 0.27622667542166823 1103 22-23 0.25644460818131876 1103 24-25 0.1940231969310986 1103 26-27 0.23521607546764756 1103 28-29 0.31084261843974303 1103 30-31 0.2685247939979192 1103 32-33 0.2784346583016344 1103 34-35 0.2953702969233447 1103 36-37 0.35077668557193675 1103 38-39 0.3474317297012277 1103 40-41 0.34486691265056635 1103 42-43 0.3312842896871757 1103 44-45 0.42029294069370593 1103 46-47 0.37802691014423573 1103 48-49 0.36711469670497365 1103 50-51 0.38936811621057643 1103 52-53 0.39947709398425246 1103 54-55 0.3696742842960248 1103 56-57 0.36771545106402925 1103 58-59 0.4374633989323371 1103 60-61 0.4319623758596407 1103 62-63 0.41929723313115375 1103 64-65 0.46402153446702954 1103 66-67 0.39467586264039056 1103 68-69 0.40557222257081804 1103 70-71 0.4647108522791257 1103 72-73 0.5241762944222828 1103 74-75 0.42155642289726103 1103 76-77 0.350540050133759 1103 78-79 0.4264690870297372 1103 80-81 0.444443242035085 1103 82-83 0.40890724679201185 1103 84-85 0.430816132126175 1103 86-87 0.3879309688303678 1103 88-89 0.4397271116424406 1103 90-91 0.39168163324558947 1103 92-93 0.4198249763259838 1103 94-95 0.441874570811315 1103 96-97 0.4580348956195017 1103 98-99 0.4910046481983876 1103 100 0.5129700297832471 1104 1 0.1918822851642581 1104 2 0.21036274927868703 1104 3 0.2744995590317245 1104 4 0.18324142678827116 1104 5 0.16143921184509225 1104 6 0.17637846712169392 1104 7 0.17746123853724782 1104 8 0.12464073094955097 1104 9 0.20001987018806489 1104 10-11 0.17048504744141724 1104 12-13 0.18431970445826806 1104 14-15 0.24402997924911318 1104 16-17 0.2599122552434352 1104 18-19 0.26620236480346904 1104 20-21 0.3085912060581748 1104 22-23 0.30238415414427067 1104 24-25 0.3386895952875406 1104 26-27 0.293285739409086 1104 28-29 0.3066892697877748 1104 30-31 0.3444129086350216 1104 32-33 0.3143605391625357 1104 34-35 0.2566177990643652 1104 36-37 0.25321979054519517 1104 38-39 0.2466597918306448 1104 40-41 0.20712666190529916 1104 42-43 0.248019593852689 1104 44-45 0.16615948131696712 1104 46-47 0.1601129909274519 1104 48-49 0.15412980787432673 1104 50-51 0.13126850136053747 1104 52-53 0.17914487289038306 1104 54-55 0.1585414379769361 1104 56-57 0.11693508950040155 1104 58-59 0.15198327182816485 1104 60-61 0.14677005826769118 1104 62-63 0.17758404115986792 1104 64-65 0.1008304991483513 1104 66-67 0.11800551450787111 1104 68-69 0.0800889131826068 1104 70-71 0.10345024661560842 1104 72-73 0.11341830244351314 1104 74-75 0.12323835360770374 1104 76-77 0.07123612796868173 1104 78-79 0.0977134068867187 1104 80-81 0.10814133687650695 1104 82-83 0.1105622684830152 1104 84-85 0.19423671308020118 1104 86-87 0.1374325669921319 1104 88-89 0.1470400471590736 1104 90-91 0.052609269042541484 1104 92-93 0.17188995107830607 1104 94-95 0.20569176731267902 1104 96-97 0.22460149193272727 1104 98-99 0.2137657007856255 1104 100 0.1969832643409788 1105 1 0.11884746471887198 1105 2 0.13777927183945593 1105 3 0.22716036506480464 1105 4 0.12176584320590678 1105 5 0.10757432939743694 1105 6 0.08072243288647485 1105 7 0.16693365140947236 1105 8 0.18198246976324128 1105 9 0.18844950437424757 1105 10-11 0.2230651565715931 1105 12-13 0.1876962701625331 1105 14-15 0.2360583378173402 1105 16-17 0.19116406244692996 1105 18-19 0.1985320188976587 1105 20-21 0.1723067652080914 1105 22-23 0.17661788284758728 1105 24-25 0.1933273733596863 1105 26-27 0.206449718934536 1105 28-29 0.2059492265858225 1105 30-31 0.19857217184723197 1105 32-33 0.2215843110978284 1105 34-35 0.2294881892617937 1105 36-37 0.23912520455156994 1105 38-39 0.28121709557890284 1105 40-41 0.27012910350904207 1105 42-43 0.3264637749386523 1105 44-45 0.3072929321287887 1105 46-47 0.3327127694773466 1105 48-49 0.2935930453669755 1105 50-51 0.2941270652463004 1105 52-53 0.2800029610335173 1105 54-55 0.2785151304182989 1105 56-57 0.3433953669723735 1105 58-59 0.35341859222766203 1105 60-61 0.32022891775920215 1105 62-63 0.33018941861284645 1105 64-65 0.2940502118989201 1105 66-67 0.31792771793764985 1105 68-69 0.3108350676997631 1105 70-71 0.3245958996414231 1105 72-73 0.34330779796697186 1105 74-75 0.3454932888069848 1105 76-77 0.32310694262748996 1105 78-79 0.3171707520402762 1105 80-81 0.32348971727189024 1105 82-83 0.3848675367241654 1105 84-85 0.3798382286671611 1105 86-87 0.3142162405651163 1105 88-89 0.26132419361238135 1105 90-91 0.3165562563254767 1105 92-93 0.3770908300412401 1105 94-95 0.3838196620158243 1105 96-97 0.3813388626652632 1105 98-99 0.4291781427044121 1105 100 0.47640379668673205 1106 1 0.12638139543410531 1106 2 0.15491669557260934 1106 3 0.2650987965195526 1106 4 0.1741335540937854 1106 5 0.14084808089809542 1106 6 0.12106549993875859 1106 7 0.13498797768865245 1106 8 0.13198374505095245 1106 9 0.18249022660730674 1106 10-11 0.1601935799983778 1106 12-13 0.1352038644764164 1106 14-15 0.1816122109052074 1106 16-17 0.18667615398229032 1106 18-19 0.20357025554121577 1106 20-21 0.17914652897203354 1106 22-23 0.16452008342738367 1106 24-25 0.1596822834861129 1106 26-27 0.2297919695095203 1106 28-29 0.19882111098710453 1106 30-31 0.20822574800317284 1106 32-33 0.1903312700741253 1106 34-35 0.1815828713120382 1106 36-37 0.19862920069736845 1106 38-39 0.25192457731565554 1106 40-41 0.20562444528858492 1106 42-43 0.25044818417994463 1106 44-45 0.1573593985606223 1106 46-47 0.20237609840824433 1106 48-49 0.15261185561638513 1106 50-51 0.1493269898039955 1106 52-53 0.10280649294526967 1106 54-55 0.10356719489410438 1106 56-57 0.14568559802420822 1106 58-59 0.16204382719086396 1106 60-61 0.0805089142607116 1106 62-63 0.12044697298237139 1106 64-65 0.11091787074919779 1106 66-67 0.07470744545605612 1106 68-69 0.16475476952675194 1106 70-71 0.1662942897006232 1106 72-73 0.18683020125398997 1106 74-75 0.1037480013859664 1106 76-77 0.11720278074315615 1106 78-79 0.17086307153454072 1106 80-81 0.1281650099650804 1106 82-83 0.18986091842070607 1106 84-85 0.15904521470299926 1106 86-87 0.14233670590343195 1106 88-89 0.15660356712852064 1106 90-91 0.1490026441585499 1106 92-93 0.18448934572361253 1106 94-95 0.10229148621961315 1106 96-97 0.1907264460536915 1106 98-99 0.17671921698527981 1106 100 0.1588292082357583 1107 1 0.09725324825717507 1107 2 0.04919086789556104 1107 3 0.18147742119086274 1107 4 0.12120520360446108 1107 5 0.12935373476960876 1107 6 0.0869051535832952 1107 7 0.05931172250020467 1107 8 0.10485076377162983 1107 9 0.1614865587743708 1107 10-11 0.11526984629415438 1107 12-13 0.08822807603721117 1107 14-15 0.14683550442118332 1107 16-17 0.13081358543679755 1107 18-19 0.15453449235911165 1107 20-21 0.06203878064476953 1107 22-23 0.06997344351488977 1107 24-25 0.11707872576059941 1107 26-27 0.18833208024474857 1107 28-29 0.14693670446855123 1107 30-31 0.17357440944938674 1107 32-33 0.156611691476499 1107 34-35 0.1522043263237407 1107 36-37 0.16010125973040346 1107 38-39 0.14149996347641292 1107 40-41 0.1255976428227612 1107 42-43 0.17611224545375848 1107 44-45 0.1008198625088852 1107 46-47 0.1937286087851433 1107 48-49 0.22853488666136457 1107 50-51 0.164507568399209 1107 52-53 0.2058257062083939 1107 54-55 0.1493425709133902 1107 56-57 0.1504082890883609 1107 58-59 0.18097617148906409 1107 60-61 0.14514781743619665 1107 62-63 0.1690926757744151 1107 64-65 0.15635770887196543 1107 66-67 0.19850209510437367 1107 68-69 0.13483621521794475 1107 70-71 0.16084493790980758 1107 72-73 0.2831646380762365 1107 74-75 0.24747185389030335 1107 76-77 0.19700463566695703 1107 78-79 0.19853017042115084 1107 80-81 0.26581252614918327 1107 82-83 0.2651721771466029 1107 84-85 0.24342435127726958 1107 86-87 0.1715217419177293 1107 88-89 0.22876222806766577 1107 90-91 0.20780377710602238 1107 92-93 0.22939690136287538 1107 94-95 0.23720896601514596 1107 96-97 0.2243864797137256 1107 98-99 0.3005855813394511 1107 100 0.29747667371249165 1108 1 0.11228398479104484 1108 2 0.08415079972396455 1108 3 0.1557275987813327 1108 4 0.09908560528444355 1108 5 0.07675859908469818 1108 6 0.05834918473505013 1108 7 0.03373522260220341 1108 8 -0.011153501293179602 1108 9 0.08654236775502255 1108 10-11 0.10336947635621385 1108 12-13 0.07793419455335027 1108 14-15 0.1514655881744389 1108 16-17 0.09044969496852673 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-0.15853048657860924 2214 100 -0.17797817710202324 2215 1 -0.28180542337756975 2215 2 -0.38838576758243804 2215 3 -0.28896197251715705 2215 4 -0.23430769689795028 2215 5 -0.2409440773697611 2215 6 -0.25075912503061915 2215 7 -0.18906723398473702 2215 8 -0.20588431144403074 2215 9 -0.4555741656840482 2215 10-11 -0.3123241523780109 2215 12-13 -0.2891295261367617 2215 14-15 -0.36497703961854455 2215 16-17 -0.41745962117848023 2215 18-19 -0.42718155151590054 2215 20-21 -0.41869138248308246 2215 22-23 -0.355017394041532 2215 24-25 -0.3484260467090152 2215 26-27 -0.4445612649124939 2215 28-29 -0.4256078333698028 2215 30-31 -0.4469018885452698 2215 32-33 -0.32054968169747156 2215 34-35 -0.42249085881216075 2215 36-37 -0.47271331386328796 2215 38-39 -0.44693560563859336 2215 40-41 -0.42656052167800595 2215 42-43 -0.3955148118238725 2215 44-45 -0.4244457756371247 2215 46-47 -0.4663865107738374 2215 48-49 -0.38734644587789546 2215 50-51 -0.43695148711283593 2215 52-53 -0.38090826624173957 2215 54-55 -0.2666799432465865 2215 56-57 -0.31143066969445954 2215 58-59 -0.287305294051059 2215 60-61 -0.3027069527428097 2215 62-63 -0.3039301361649791 2215 64-65 -0.30479420298824067 2215 66-67 -0.25833262423034853 2215 68-69 -0.2628905079940864 2215 70-71 -0.35317503694437846 2215 72-73 -0.3084223571440319 2215 74-75 -0.24453062360534972 2215 76-77 -0.15674330369220257 2215 78-79 -0.2663334945999125 2215 80-81 -0.4071611103737709 2215 82-83 -0.34146627682921604 2215 84-85 -0.30481671406768385 2215 86-87 -0.24147118482126473 2215 88-89 -0.3207213028035909 2215 90-91 -0.2992419511228164 2215 92-93 -0.29297295012555225 2215 94-95 -0.3393946434058819 2215 96-97 -0.34663302029318643 2215 98-99 -0.3058590796828611 2215 100 -0.3473246576051032 2216 1 -0.3204828689868542 2216 2 -0.35025445505023 2216 3 -0.4278687148210558 2216 4 -0.22966365557667245 2216 5 -0.3652315417813554 2216 6 -0.2802931024033697 2216 7 -0.5424739132793164 2216 8 -0.29503771463792816 2216 9 -0.45629905193583653 2216 10-11 -0.40388046609993467 2216 12-13 -0.345751142421328 2216 14-15 -0.6165471002568097 2216 16-17 -0.49255200344281747 2216 18-19 -0.36293061820348527 2216 20-21 -0.42229231608159523 2216 22-23 -0.4744511175209283 2216 24-25 -0.4307297597789628 2216 26-27 -0.5401966817725068 2216 28-29 -0.5636706485646599 2216 30-31 -0.632508512659598 2216 32-33 -0.5779578817285369 2216 34-35 -0.544392569302282 2216 36-37 -0.6184502259629951 2216 38-39 -0.6126500820953638 2216 40-41 -0.5901839156905382 2216 42-43 -0.624278596245766 2216 44-45 -0.7639628081788175 2216 46-47 -0.7118901715244235 2216 48-49 -0.7417160931387627 2216 50-51 -0.6949156327183701 2216 52-53 -0.5749379991729526 2216 54-55 -0.5480864416772206 2216 56-57 -0.6504391183157736 2216 58-59 -0.6694453294311771 2216 60-61 -0.4611749129588816 2216 62-63 -0.3921321981117174 2216 64-65 -0.5749849732886432 2216 66-67 -0.45695444894108306 2216 68-69 -0.44216322173626565 2216 70-71 -0.5200638469223691 2216 72-73 -0.5089996337794886 2216 74-75 -0.500438549479604 2216 76-77 -0.49747665852174094 2216 78-79 -0.721101841510368 2216 80-81 -0.8534029203837363 2216 82-83 -0.6644164731628948 2216 84-85 -0.8522274219114152 2216 86-87 -0.6395355776912623 2216 88-89 -0.8915846556919043 2216 90-91 -0.6713788562726464 2216 92-93 -0.7808160246899227 2216 94-95 -0.7610022092408499 2216 96-97 -0.7244655437625873 2216 98-99 -0.8756568912988207 2216 100 -0.7796018882709781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 252.0 3 24.0 4 40.0 5 77.0 6 183.0 7 477.0 8 1314.0 9 2322.0 10 3244.0 11 4593.0 12 6456.0 13 9155.0 14 11361.0 15 14281.0 16 17835.0 17 24263.0 18 24037.0 19 13656.0 20 10675.0 21 10638.0 22 11329.0 23 12196.0 24 14847.0 25 18407.0 26 23639.0 27 31148.0 28 40909.0 29 53777.0 30 69658.0 31 90817.0 32 117855.0 33 154421.0 34 209941.0 35 303124.0 36 490232.0 37 903672.0 38 1333040.0 39 577542.0 40 2782.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.251978649555554 20.68128636015856 14.681498398976837 23.385236591309052 2 15.193470444294041 20.576721651052974 40.197073437563326 24.032734467089664 3 17.744476367506614 25.372636192603775 30.902889524749476 25.979997915140135 4 12.298367609304641 17.20579062869646 38.775899860734675 31.719941901264225 5 12.482177373895777 37.96486469324494 35.946776691786845 13.606181241072433 6 30.52408880551374 38.47570704288354 17.348096687239313 13.652107464363409 7 26.315395953247993 33.102958485498846 22.563840164500213 18.01780539675295 8 24.68431051544086 36.696179997945464 20.29233325227895 18.327176234334726 9 24.475062843787864 16.57836353237677 21.88981493942962 37.05675868440574 10-11 23.52702591706202 27.241966625337895 28.918360832028128 20.312646625571958 12-13 24.11283349507785 24.683893541753935 29.398811242848332 21.804461720319882 14-15 21.348719559682273 26.73010254076886 26.993798618117694 24.92737928143117 16-17 20.414039732401086 30.21916601704427 27.709358831906332 21.657435418648312 18-19 20.285250997081413 28.59743050662767 30.583715503627158 20.53360299266376 20-21 21.327985510859975 27.750953060374634 30.443285633990357 20.477775794775038 22-23 20.984125374196587 27.5659867899638 30.52835160186372 20.92153623397589 24-25 20.89040743346618 27.721929208520585 30.443443936870278 20.944219421142954 26-27 20.709587577780013 27.734053600227384 30.500396004477903 21.0559628175147 28-29 20.64995133508315 27.70499893047711 30.671088228161413 20.97396150627833 30-31 20.95885565899668 28.0178400721769 30.253765588499377 20.76953868032705 32-33 20.691052281002843 27.814872731721437 30.598989924493026 20.89508506278269 34-35 20.830697909385048 27.647109753085925 30.549227588259026 20.97296474927 36-37 21.08543864696452 27.97093981807316 30.04854019126753 20.895081343694788 38-39 20.980241896334107 28.055031329320222 30.082854955823407 20.881871818522267 40-41 20.88834679554676 27.86283203533258 30.205399123989007 21.043422045131656 42-43 20.90102923561414 27.872832154472682 30.072660467110126 21.153478142803056 44-45 21.03305246901473 28.016947216738558 29.8414202737674 21.10858004047931 46-47 21.156316364242397 28.174674641157658 29.502690924757918 21.16631806984203 48-49 21.151311326688024 28.157352700868753 29.267495300358764 21.423840672084456 50-51 21.250369375696287 28.218840322313415 29.06887850332598 21.46191179866432 52-53 21.1663989781095 28.274565021445785 29.125548064417767 21.43348793602695 54-55 21.302456925113276 28.5200421958074 28.903499575495438 21.27400130358388 56-57 21.15916377215797 28.311459176970555 29.016966549109195 21.51241050176228 58-59 21.106764639272335 27.985876254140734 29.2925448857498 21.614814220837133 60-61 21.245152883617735 28.29101390540639 29.04551968510336 21.41831352587251 62-63 21.086029159425863 28.321574847825254 29.093260338146354 21.49913565460253 64-65 21.118936117899644 28.108226016864695 29.213066503142315 21.55977136209335 66-67 21.08114568843264 28.14968255810413 29.054485965621197 21.714685787842033 68-69 21.375034134204856 28.275442444312482 28.90457376672576 21.4449496547569 70-71 21.356406016989723 28.173436744519798 28.917227489656387 21.55292974883409 72-73 21.167903776650313 28.31831604370136 28.941546837721923 21.572233341926406 74-75 21.347155503094104 28.301173241668103 28.82169892970183 21.529972325535958 76-77 21.31762714072041 28.31292002916656 28.676461744031478 21.692991086081552 78-79 21.348363309773504 28.41800337576299 28.71599421595 21.517639098513502 80-81 21.324292339482863 28.274193343894456 28.768775758710618 21.632738557912067 82-83 21.19559104346995 28.338928076290493 28.894382132538738 21.571098747700823 84-85 21.275436251268943 28.263150389112983 28.72377269247807 21.737640667140003 86-87 21.327867969572285 28.37791198094296 28.657075081133605 21.637144968351148 88-89 21.204374911142473 28.231910774906982 28.710408553902276 21.85330576004827 90-91 21.137325882382136 28.179348176502543 28.792470622299778 21.890855318815543 92-93 21.207906200559158 28.357751240762013 28.663520513209512 21.770822045469323 94-95 21.09079418497975 28.166756284228327 28.770152302174377 21.972297228617542 96-97 21.10402529653863 28.26284603397868 28.682453885615082 21.950674783867605 98-99 21.108037431465302 28.28342384602281 28.648317348224545 21.960221374287343 100 21.16667208478268 28.20536826934538 28.60767421952039 22.02028542635155 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 533.0 1 404.5 2 481.0 3 696.0 4 451.5 5 272.0 6 778.0 7 2171.0 8 3709.0 9 4215.0 10 3173.5 11 1994.5 12 1888.0 13 2159.5 14 2475.5 15 2869.5 16 3403.5 17 3976.5 18 4745.5 19 5739.0 20 7154.5 21 9288.0 22 11939.5 23 15715.0 24 21156.0 25 28164.0 26 37522.5 27 48833.5 28 61930.0 29 77194.5 30 92402.5 31 105739.0 32 116199.5 33 125467.5 34 135800.0 35 144620.5 36 151295.5 37 158095.5 38 164106.5 39 166860.5 40 168235.5 41 168797.0 42 167764.0 43 165611.0 44 161507.5 45 156887.0 46 152289.5 47 146029.5 48 139581.0 49 135281.5 50 131408.5 51 126503.5 52 118875.0 53 111860.0 54 105838.5 55 99444.5 56 92421.0 57 83658.5 58 76022.5 59 69606.0 60 61357.5 61 51465.0 62 43380.0 63 36133.0 64 28809.0 65 22523.0 66 17488.5 67 13299.0 68 10222.0 69 7853.5 70 5760.5 71 4177.5 72 2894.5 73 1953.5 74 1401.5 75 935.5 76 597.5 77 374.5 78 241.0 79 175.5 80 103.0 81 45.0 82 20.5 83 12.5 84 6.0 85 3.0 86 3.0 87 2.5 88 2.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04022349177618141 2 0.0 3 0.0 4 1.0836069982807491E-4 5 0.0 6 2.1672139965614984E-5 7 0.0 8 7.151806188652945E-4 9 0.0 10-11 0.0 12-13 0.04349598491098927 14-15 1.191967698108824E-4 16-17 0.0 18-19 4.334427993122997E-5 20-21 9.752462984526741E-5 22-23 0.0 24-25 1.191967698108824E-4 26-27 7.585248987965245E-5 28-29 2.600656795873798E-4 30-31 0.0 32-33 2.0588532967334233E-4 34-35 5.4180349914037456E-5 36-37 3.250820994842247E-5 38-39 2.383935396217648E-4 40-41 1.6254104974211235E-4 42-43 3.9009851938106964E-4 44-45 2.1672139965614982E-4 46-47 1.300328397936899E-4 48-49 8.018691787277543E-4 50-51 3.1424602950141727E-4 52-53 5.634756391059895E-4 54-55 3.575903094326472E-4 56-57 4.767870792435296E-4 58-59 8.668855986245993E-5 60-61 1.4086890977649738E-4 62-63 7.260166888481019E-4 64-65 5.092952891919521E-4 66-67 0.0014628694476790114 68-69 0.0017120990572835836 70-71 3.9009851938106964E-4 72-73 8.127052487105618E-4 74-75 8.127052487105618E-4 76-77 6.610002689512569E-4 78-79 0.0032724931348078624 80-81 0.0026981814257190655 82-83 0.001430361237730589 84-85 0.0029257388953580227 86-87 0.0015278858675758565 88-89 0.0019721647368709633 90-91 0.0028715585454439853 92-93 0.002578984655908183 94-95 0.0036409195142233174 96-97 0.0032291488548766324 98-99 0.002882394615426793 100 0.0014953776576274338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4614219.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.950195066134484 #Duplication Level Percentage of deduplicated Percentage of total 1 76.99523454651843 49.238602684126306 2 14.209710631494515 18.174275334347985 3 4.164380140704871 7.989387669828392 4 1.7362918884999776 4.441448198452824 5 0.8935446271179321 2.857117660224408 6 0.493512211958829 1.8936121333371974 7 0.32867790113113593 1.4713311123884667 8 0.22580631635232518 1.1552286382317174 9 0.15029001871452957 0.8649968411958453 >10 0.755865089126492 8.335110980389304 >50 0.034751799357739185 1.5066908319662686 >100 0.011223337318444104 1.165534400395242 >500 5.082085098209358E-4 0.22942851660577557 >1k 1.0164159748148807E-4 0.11429166641594381 >5k 6.776106498679844E-5 0.3347499992631464 >10k+ 3.388053249339922E-5 0.22819333283103288 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10532 0.228250978117857 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9731 0.21089159400539942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5719 0.1239429684633521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.334427993122997E-5 0.0 2 0.0 0.0 0.0 4.334427993122997E-5 0.0 3 0.0 0.0 0.0 4.334427993122997E-5 0.0 4 0.0 0.0 0.0 1.300328397936899E-4 0.0 5 0.0 0.0 0.0 1.517049797593049E-4 0.0 6 0.0 0.0 0.0 1.517049797593049E-4 0.0 7 0.0 0.0 0.0 1.517049797593049E-4 0.0 8 0.0 0.0 0.0 1.517049797593049E-4 0.0 9 0.0 0.0 0.0 1.9504925969053485E-4 0.0 10-11 0.0 0.0 0.0 2.1672139965614982E-4 0.0 12-13 0.0 0.0 0.0 2.1672139965614982E-4 0.0 14-15 0.0 0.0 0.0 2.383935396217648E-4 0.0 16-17 0.0 0.0 0.0 2.817378195529948E-4 0.0 18-19 0.0 0.0 0.0 3.684263794154547E-4 0.0 20-21 0.0 0.0 0.0 4.659510092607221E-4 0.0 22-23 0.0 0.0 0.0 5.418034991403745E-4 0.0 24-25 0.0 0.0 0.0 7.260166888481019E-4 0.0 26-27 0.0 0.0 0.0 0.0011594594881604017 0.0 28-29 0.0 0.0 0.0 0.00271985356568468 0.0 30-31 0.0 0.0 0.0 0.007953675367380699 0.0 32-33 0.0 0.0 0.0 0.018193761501133776 0.0 34-35 0.0 0.0 0.0 0.03436117791548256 0.0 36-37 0.0 0.0 0.0 0.05935999136581944 0.0 38-39 0.0 0.0 0.0 0.09293013617255705 0.0 40-41 0.0 0.0 0.0 0.12647860883932904 0.0 42-43 0.0 0.0 0.0 0.15935524516716698 0.0 44-45 0.0 0.0 0.0 0.20203852482944568 0.0 46-47 0.0 0.0 0.0 0.25375908685738585 0.0 48-49 0.0 0.0 0.0 0.3061189770143116 0.0 50-51 0.0 0.0 0.0 0.3577961947623206 0.0 52-53 0.0 0.0 0.0 0.40888826473125783 0.0 54-55 0.0 0.0 0.0 0.4659293371207565 0.0 56-57 0.0 0.0 0.0 0.5309565930875842 0.0 58-59 0.0 0.0 0.0 0.6018569989850937 0.0 60-61 0.0 0.0 0.0 0.6765825375865342 0.0 62-63 0.0 0.0 0.0 0.7490108293516193 0.0 64-65 0.0 0.0 0.0 0.8281033041561312 0.0 66-67 0.0 0.0 0.0 0.9141416998196228 0.0 68-69 0.0 0.0 0.0 1.0116013132449932 0.0 70-71 0.0 0.0 0.0 1.1132328136137448 0.0 72-73 0.0 0.0 0.0 1.216457216269969 0.0 74-75 0.0 2.1672139965614984E-5 0.0 1.3207218816445425 0.0 76-77 0.0 2.1672139965614984E-5 0.0 1.43445727218409 0.0 78-79 0.0 2.1672139965614984E-5 0.0 1.5629297179002557 0.0 80-81 0.0 2.1672139965614984E-5 0.0 1.69602916549908 0.0 82-83 0.0 2.1672139965614984E-5 0.0 1.8339506642402539 0.0 84-85 0.0 2.1672139965614984E-5 0.0 1.9738876719982297 0.0 86-87 0.0 2.1672139965614984E-5 0.0 2.126524553776056 0.0 88 0.0 2.1672139965614984E-5 0.0 2.2473358980143767 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6630 0.0 57.15421 1 GTATCAA 12530 0.0 41.91108 1 TCAACGC 15265 0.0 33.742382 4 ATCAACG 15345 0.0 33.62772 3 CAACGCA 15810 0.0 32.608944 5 TATCAAC 16215 0.0 32.055325 2 AACGCAG 16495 0.0 31.305988 6 ACGCAGA 18905 0.0 27.066343 7 CGCAGAG 19130 0.0 26.600492 8 GTGGTAT 3125 0.0 24.522474 1 GCAGAGT 21185 0.0 23.686924 9 GTACATG 11535 0.0 23.313374 1 TGGTATC 3095 0.0 21.86572 2 TACATGG 11905 0.0 21.553865 2 GAGTACT 14865 0.0 21.268766 12-13 AGAGTAC 19460 0.0 20.892714 10-11 CAGAGTA 20885 0.0 20.503036 10-11 ACATGGG 12150 0.0 20.461683 3 AGTACTT 16240 0.0 18.671904 12-13 GTACTTT 17055 0.0 18.420734 14-15 >>END_MODULE