FastQCFastQC Report
Wed 25 May 2016
SRR2049382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735469
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46450.040.5868571
GTATCAA77400.035.7160261
TCAACGC81300.033.1903154
ATCAACG81750.033.0076183
CAACGCA85600.031.5230485
AACGCAG89100.030.2790096
TATCAAC93200.029.2046972
ACGCAGA104900.025.3599517
GTACATG130250.025.158331
CGCAGAG107250.024.4975138
TACATGG135800.023.0895862
ACATGGG140950.021.5788973
GCAGAGT127550.020.0090689
CATGGGG104600.019.1006134
GTGGTAT29950.018.993951
AGAGTAC108150.017.57921410-11
GAGTACA97050.017.1972711
CAGAGTA118550.016.8894510-11
GTATAGG17150.016.7221641
ACCGCGT2901.1510656E-616.2072568
Produced by FastQC (version 0.11.5)