##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049382_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4735469
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.936345270130584	31.0	31.0	34.0	28.0	34.0
2	31.04070895617731	31.0	31.0	34.0	28.0	34.0
3	31.210478624186962	31.0	31.0	34.0	28.0	34.0
4	34.813151770183694	37.0	35.0	37.0	32.0	37.0
5	34.82760778288275	37.0	35.0	37.0	32.0	37.0
6	34.8527662201991	37.0	35.0	37.0	32.0	37.0
7	34.7272692525281	37.0	35.0	37.0	32.0	37.0
8	34.71527762086501	37.0	35.0	37.0	32.0	37.0
9	36.3109081698138	39.0	37.0	39.0	32.0	39.0
10-11	36.30195963694409	39.0	37.0	39.0	32.0	39.0
12-13	36.26495020873328	39.0	37.0	39.0	32.0	39.0
14-15	37.48404318558521	40.0	38.0	41.0	32.0	41.0
16-17	37.44137349436772	40.0	38.0	41.0	32.0	41.0
18-19	37.455576100276446	40.0	38.0	41.0	32.0	41.0
20-21	37.53726494672439	40.0	38.0	41.0	32.0	41.0
22-23	37.48062979611946	40.0	38.0	41.0	32.0	41.0
24-25	37.476687631151215	40.0	38.0	41.0	32.0	41.0
26-27	37.4654757532992	40.0	38.0	41.0	32.0	41.0
28-29	37.408759829279845	40.0	38.0	41.0	32.0	41.0
30-31	37.34844742938873	40.0	38.0	41.0	32.0	41.0
32-33	37.303127631075185	40.0	37.5	41.0	31.0	41.0
34-35	37.20761787269645	40.0	37.0	41.0	31.0	41.0
36-37	37.128249810103284	40.0	37.0	41.0	31.0	41.0
38-39	37.02823849126665	40.0	37.0	41.0	30.5	41.0
40-41	36.93163855575868	40.0	37.0	41.0	30.0	41.0
42-43	36.79101858759924	40.0	37.0	41.0	30.0	41.0
44-45	36.659951633090614	40.0	37.0	41.0	30.0	41.0
46-47	36.54114851137237	40.0	36.0	41.0	30.0	41.0
48-49	36.41538652243315	40.0	36.0	41.0	30.0	41.0
50-51	36.276642398039144	40.0	36.0	41.0	29.5	41.0
52-53	36.137138686791104	39.0	35.5	41.0	29.0	41.0
54-55	35.9984957139409	39.0	35.0	41.0	29.0	41.0
56-57	35.870237984875416	39.0	35.0	41.0	29.0	41.0
58-59	35.66772805396889	39.0	35.0	41.0	28.0	41.0
60-61	35.45187118741565	39.0	35.0	40.0	28.0	41.0
62-63	35.240685558283666	38.0	35.0	40.0	28.0	41.0
64-65	34.993324842798046	38.0	34.0	40.0	28.0	41.0
66-67	34.72925268859325	37.5	34.0	40.0	27.5	41.0
68-69	34.43749974923286	37.0	34.0	40.0	27.0	41.0
70-71	34.14999285181679	36.5	34.0	39.0	26.0	41.0
72-73	33.802255172613314	36.0	34.0	39.0	26.0	41.0
74-75	33.45038991914001	35.5	33.5	39.0	26.0	40.0
76-77	33.115113202092544	35.0	33.0	37.5	26.0	40.0
78-79	32.80207725992927	35.0	33.0	37.0	26.0	39.0
80-81	32.45184236239325	35.0	33.0	37.0	25.0	39.0
82-83	32.146622224746906	35.0	33.0	36.0	25.0	39.0
84-85	31.865881605391145	35.0	33.0	36.0	24.5	37.0
86-87	31.561163213189655	35.0	32.5	35.5	23.5	37.0
88-89	31.325952297438754	35.0	32.0	35.0	23.0	36.5
90-91	31.13585623725971	35.0	32.0	35.0	22.0	36.0
92-93	30.914109985726864	35.0	32.0	35.0	20.0	36.0
94-95	30.729564273359195	35.0	32.0	35.0	19.5	36.0
96-97	30.49146219730295	35.0	32.0	35.0	15.5	35.5
98-99	30.3003877757409	35.0	32.0	35.0	2.0	35.0
100	30.187545309662042	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.36803734789942055
1101	2	0.3608885649237017
1101	3	0.40798896250026573
1101	4	0.43003762777500043
1101	5	0.33333426594722937
1101	6	0.4431493692112696
1101	7	0.404465634482861
1101	8	0.49787209910572017
1101	9	0.5520572850999557
1101	10-11	0.5729249697127017
1101	12-13	0.626048391954555
1101	14-15	0.7172835001128632
1101	16-17	0.7290810640165759
1101	18-19	0.6934143997679172
1101	20-21	0.6946129908174754
1101	22-23	0.6612765062358932
1101	24-25	0.7021749701184845
1101	26-27	0.5752506051310391
1101	28-29	0.6285881612217636
1101	30-31	0.6606411927329248
1101	32-33	0.6746798391666786
1101	34-35	0.7334408165905018
1101	36-37	0.667692737657795
1101	38-39	0.7610861138131852
1101	40-41	0.7781458903569813
1101	42-43	0.7293866547593026
1101	44-45	0.6562249953604322
1101	46-47	0.5997681611206716
1101	48-49	0.5311766244099161
1101	50-51	0.6219245047084812
1101	52-53	0.609901945397624
1101	54-55	0.6219111692034645
1101	56-57	0.5178233004525694
1101	58-59	0.6487721766416854
1101	60-61	0.6489603071658365
1101	62-63	0.6360956815952576
1101	64-65	0.6102293416857734
1101	66-67	0.6251547767872552
1101	68-69	0.5772543214977972
1101	70-71	0.6448680209016118
1101	72-73	0.6851330766780563
1101	74-75	0.6462942176134092
1101	76-77	0.6415214084139933
1101	78-79	0.5579077919571063
1101	80-81	0.6121365448321896
1101	82-83	0.6327802285109527
1101	84-85	0.5557913071741751
1101	86-87	0.6255596642996188
1101	88-89	0.6157901940392989
1101	90-91	0.5920218465934752
1101	92-93	0.5646652426541188
1101	94-95	0.5921399635536169
1101	96-97	0.5973468598696421
1101	98-99	0.7055610388475557
1101	100	0.7315574420539583
1102	1	0.28978948192245113
1102	2	0.374579850237879
1102	3	0.37904748744929506
1102	4	0.4325433698380934
1102	5	0.3696738685230443
1102	6	0.533012776097749
1102	7	0.5494701590591546
1102	8	0.48572562099854366
1102	9	0.6653687927287493
1102	10-11	0.589016126783477
1102	12-13	0.6336639619707967
1102	14-15	0.7581201796342754
1102	16-17	0.7533315113696588
1102	18-19	0.7271671198354426
1102	20-21	0.6839569294274668
1102	22-23	0.7069452290542273
1102	24-25	0.7485021349968193
1102	26-27	0.7396459801148012
1102	28-29	0.6932588337566941
1102	30-31	0.7137353686496652
1102	32-33	0.7814031140823658
1102	34-35	0.7925637677698205
1102	36-37	0.7421498210850714
1102	38-39	0.8067675436748658
1102	40-41	0.8688338866104672
1102	42-43	0.8217066698079165
1102	44-45	0.7180746934106708
1102	46-47	0.6563520278240063
1102	48-49	0.6916876517341777
1102	50-51	0.7602334926500376
1102	52-53	0.8200835996417908
1102	54-55	0.7325600299355628
1102	56-57	0.7023078919135202
1102	58-59	0.7990198119369367
1102	60-61	0.703561027970153
1102	62-63	0.7492747535944488
1102	64-65	0.7204162851009883
1102	66-67	0.8142046613812397
1102	68-69	0.8040105869390217
1102	70-71	0.7985879155265536
1102	72-73	0.7658877273473124
1102	74-75	0.7921547299005809
1102	76-77	0.8672571679454748
1102	78-79	0.804761365112526
1102	80-81	0.743733281489682
1102	82-83	0.7880108602617071
1102	84-85	0.8380106672344425
1102	86-87	0.8578456005784609
1102	88-89	0.8162444002444218
1102	90-91	0.8833919069747367
1102	92-93	0.8142292824599657
1102	94-95	0.8915437751453119
1102	96-97	0.8318092737386955
1102	98-99	0.8612495368663424
1102	100	0.838381298105098
1103	1	0.13148526815061246
1103	2	0.2189354095222278
1103	3	0.2518683444663061
1103	4	0.22188665905511584
1103	5	0.25991843089064304
1103	6	0.31796338121132806
1103	7	0.31457786298347656
1103	8	0.3257774279494896
1103	9	0.4053494962126081
1103	10-11	0.43778576413482284
1103	12-13	0.443076571824605
1103	14-15	0.5764050968899781
1103	16-17	0.528169064068031
1103	18-19	0.487741505807719
1103	20-21	0.4422089167588865
1103	22-23	0.3991565678549094
1103	24-25	0.42970984521348754
1103	26-27	0.4313611677314739
1103	28-29	0.4511912749757343
1103	30-31	0.47087780630304366
1103	32-33	0.4648952591418336
1103	34-35	0.5096649905816548
1103	36-37	0.4516137135279692
1103	38-39	0.4185700899760221
1103	40-41	0.45186574683657454
1103	42-43	0.45729825484892217
1103	44-45	0.38388860787676293
1103	46-47	0.4124401835385001
1103	48-49	0.503952685188473
1103	50-51	0.37272033116756376
1103	52-53	0.43302369654386297
1103	54-55	0.43766679000855646
1103	56-57	0.43137201088073596
1103	58-59	0.44582592389868125
1103	60-61	0.42081976908222174
1103	62-63	0.4146159034151182
1103	64-65	0.4524222838005656
1103	66-67	0.4738442150444371
1103	68-69	0.5291479697441517
1103	70-71	0.43494664195648625
1103	72-73	0.448380124100467
1103	74-75	0.4203231339528557
1103	76-77	0.5340556242241803
1103	78-79	0.41336750643853293
1103	80-81	0.3858868356143148
1103	82-83	0.38399487771246754
1103	84-85	0.4438888684635458
1103	86-87	0.4603731295756752
1103	88-89	0.4136914921335908
1103	90-91	0.48219940325176935
1103	92-93	0.4441217882803805
1103	94-95	0.4689112689054049
1103	96-97	0.46809574310547575
1103	98-99	0.5174938475402548
1103	100	0.3837799179847039
1104	1	0.18672970052156046
1104	2	0.195284465194959
1104	3	0.26472788118991986
1104	4	0.30412128035256103
1104	5	0.3350145220279117
1104	6	0.355039858341037
1104	7	0.4129786875084136
1104	8	0.44414101455156896
1104	9	0.4850590811115083
1104	10-11	0.4712545885661541
1104	12-13	0.46290487040762685
1104	14-15	0.6024513553435398
1104	16-17	0.6041643798222154
1104	18-19	0.520824795645936
1104	20-21	0.5503715915405678
1104	22-23	0.4911330977892163
1104	24-25	0.5789607321577321
1104	26-27	0.5380372761446992
1104	28-29	0.5439188510820117
1104	30-31	0.5548108533316878
1104	32-33	0.5348837723618018
1104	34-35	0.4940402023341903
1104	36-37	0.5171342254171449
1104	38-39	0.6027006099130645
1104	40-41	0.5830438387287771
1104	42-43	0.5575129833630967
1104	44-45	0.48911888780217083
1104	46-47	0.4824751220704897
1104	48-49	0.4496113436668665
1104	50-51	0.488481617768322
1104	52-53	0.4804153224879144
1104	54-55	0.439235666430541
1104	56-57	0.44695594933031657
1104	58-59	0.547060829058978
1104	60-61	0.46234586259010513
1104	62-63	0.5555170407996499
1104	64-65	0.5433270737573181
1104	66-67	0.5970169674661747
1104	68-69	0.5806416072482321
1104	70-71	0.5771423465722592
1104	72-73	0.613270582544061
1104	74-75	0.667520552937475
1104	76-77	0.6570442422394009
1104	78-79	0.5809763841221383
1104	80-81	0.6461831619813125
1104	82-83	0.5531930007583625
1104	84-85	0.5483684481430373
1104	86-87	0.6133815060734626
1104	88-89	0.6160295758456584
1104	90-91	0.6504635971575681
1104	92-93	0.6147790477930926
1104	94-95	0.6507033490300174
1104	96-97	0.5832286753680656
1104	98-99	0.6157066612519451
1104	100	0.5813012153295176
1105	1	0.14157519620816572
1105	2	0.1649535949738663
1105	3	0.16451922376286987
1105	4	0.22044181493041748
1105	5	0.247031740243159
1105	6	0.31128172653506425
1105	7	0.22084285099307266
1105	8	0.2780596022100781
1105	9	0.3654064506490542
1105	10-11	0.3526159000261089
1105	12-13	0.3510127229037323
1105	14-15	0.3883860121577598
1105	16-17	0.4193286364101567
1105	18-19	0.4087447032497664
1105	20-21	0.35612428446471966
1105	22-23	0.4034041697733741
1105	24-25	0.36336142395050075
1105	26-27	0.38279102384657904
1105	28-29	0.41887512789340065
1105	30-31	0.4235541652159114
1105	32-33	0.4626715381186486
1105	34-35	0.5113526404617517
1105	36-37	0.4680420708421167
1105	38-39	0.4098530635971187
1105	40-41	0.46073864851523183
1105	42-43	0.5050985146410838
1105	44-45	0.39662142162575975
1105	46-47	0.3892707191100371
1105	48-49	0.4943069018151718
1105	50-51	0.4713699115032952
1105	52-53	0.43184663819054947
1105	54-55	0.4405265022387752
1105	56-57	0.39218985660416195
1105	58-59	0.43745012453816656
1105	60-61	0.38770825829085
1105	62-63	0.3735357675238262
1105	64-65	0.42365729579097433
1105	66-67	0.4545646387055058
1105	68-69	0.396980703557098
1105	70-71	0.41392845650484844
1105	72-73	0.40349503217400695
1105	74-75	0.4553675983813079
1105	76-77	0.53363795835687
1105	78-79	0.3659439452874551
1105	80-81	0.4185077388916909
1105	82-83	0.4414988745150197
1105	84-85	0.41374398437081794
1105	86-87	0.41601311758526904
1105	88-89	0.4313253850192851
1105	90-91	0.436234675034342
1105	92-93	0.4382894541476894
1105	94-95	0.3989502377303431
1105	96-97	0.41920885261786367
1105	98-99	0.45261575001827126
1105	100	0.41916361103026745
1106	1	0.18385661697319478
1106	2	0.17608975162231744
1106	3	0.25903880481933683
1106	4	0.28283252531292646
1106	5	0.2606451282212987
1106	6	0.3319306336388905
1106	7	0.316894763561379
1106	8	0.28717617780206695
1106	9	0.35777666922829354
1106	10-11	0.33767509856891564
1106	12-13	0.33126887044471687
1106	14-15	0.38207567518144003
1106	16-17	0.4099581901442164
1106	18-19	0.3997100948708905
1106	20-21	0.3941900859219203
1106	22-23	0.4360202899919514
1106	24-25	0.4222742199432119
1106	26-27	0.39721042275368035
1106	28-29	0.4943441625017826
1106	30-31	0.4625580358898631
1106	32-33	0.41956247618786335
1106	34-35	0.44161903390437374
1106	36-37	0.37745805444867386
1106	38-39	0.40527656086488406
1106	40-41	0.4551922004277955
1106	42-43	0.45819163850265454
1106	44-45	0.43427397171684134
1106	46-47	0.42000387029401054
1106	48-49	0.40787816775323193
1106	50-51	0.4887025617765133
1106	52-53	0.4758129405584839
1106	54-55	0.4573571033316739
1106	56-57	0.367901377551334
1106	58-59	0.3706411536820653
1106	60-61	0.39740771184276724
1106	62-63	0.390532579909987
1106	64-65	0.4067447274849698
1106	66-67	0.4652864055543233
1106	68-69	0.4714957308795036
1106	70-71	0.45396716324437136
1106	72-73	0.5471539574927675
1106	74-75	0.5015572614049901
1106	76-77	0.49468531719111297
1106	78-79	0.3802301474732488
1106	80-81	0.3572349702213842
1106	82-83	0.3996318435496278
1106	84-85	0.460358283095772
1106	86-87	0.4631135274213314
1106	88-89	0.4897771154382262
1106	90-91	0.521745831682793
1106	92-93	0.4779456472260115
1106	94-95	0.5600226421019272
1106	96-97	0.49959592001312814
1106	98-99	0.5240046389071615
1106	100	0.5496280919045837
1107	1	0.20245084458381513
1107	2	0.14395885022912225
1107	3	0.1759275639212099
1107	4	0.2266436986323015
1107	5	0.16351174297316362
1107	6	0.2212063102096522
1107	7	0.2294839921342131
1107	8	0.29098207013254296
1107	9	0.33414894314154964
1107	10-11	0.2817880266982371
1107	12-13	0.32251132377733427
1107	14-15	0.32825753140428304
1107	16-17	0.4018093557658702
1107	18-19	0.31382387332892847
1107	20-21	0.3025751102905474
1107	22-23	0.3028530504722511
1107	24-25	0.3253568853209572
1107	26-27	0.36584487315042935
1107	28-29	0.41319057970885353
1107	30-31	0.38987395091069743
1107	32-33	0.44291160773371274
1107	34-35	0.42360045645956745
1107	36-37	0.381258184683233
1107	38-39	0.4468787256227884
1107	40-41	0.44639521417179395
1107	42-43	0.4048799437975177
1107	44-45	0.38572495697929554
1107	46-47	0.36822103306035814
1107	48-49	0.4096507115339776
1107	50-51	0.499802036810415
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2209	6	0.24591131945074096
2209	7	0.27813930883076665
2209	8	0.22084795360667897
2209	9	0.2865414625443776
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2209	22-23	0.36162020202290535
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2212	60-61	-0.27467535738223603
2212	62-63	-0.2634662763861044
2212	64-65	-0.3410625197183137
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2212	78-79	-0.29401232976749725
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2212	96-97	-0.1862687900966229
2212	98-99	-0.21234929876256814
2212	100	-0.28538076588253247
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2214	4	-0.20685798486938722
2214	5	-0.21421825975684072
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2214	86-87	-0.09834936990221976
2214	88-89	-0.06499343379953615
2214	90-91	-0.13544763227296386
2214	92-93	-0.0692468321385995
2214	94-95	-0.11878875125864496
2214	96-97	-0.15619655278754152
2214	98-99	-0.11368304878052982
2214	100	-0.07730786044120563
2215	1	-0.26937696139033207
2215	2	-0.2826326568501898
2215	3	-0.3389858314799916
2215	4	-0.3488030255949113
2215	5	-0.3392807988437667
2215	6	-0.31937884257062166
2215	7	-0.38313932536715356
2215	8	-0.3142345399621149
2215	9	-0.4962422360301204
2215	10-11	-0.48195935534182865
2215	12-13	-0.49124532900370355
2215	14-15	-0.6449497966810966
2215	16-17	-0.5244152399709137
2215	18-19	-0.5848148131146331
2215	20-21	-0.5352686434068659
2215	22-23	-0.6354755988320022
2215	24-25	-0.6134473888491669
2215	26-27	-0.5765171375042613
2215	28-29	-0.5413974382937994
2215	30-31	-0.5554101614465878
2215	32-33	-0.5876269557316789
2215	34-35	-0.6336106178246723
2215	36-37	-0.6924015658057883
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2215	44-45	-0.5174313717867847
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2215	54-55	-0.5721360993872437
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2215	62-63	-0.3494851309543847
2215	64-65	-0.39412569357737937
2215	66-67	-0.3337152758244102
2215	68-69	-0.36102664478561763
2215	70-71	-0.28251593582368884
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2215	80-81	-0.2941546021547907
2215	82-83	-0.3238737542112631
2215	84-85	-0.2984826677033965
2215	86-87	-0.22854480924399923
2215	88-89	-0.2411428242781959
2215	90-91	-0.3058711984282354
2215	92-93	-0.25518276807453333
2215	94-95	-0.32439794863613614
2215	96-97	-0.2892813495918496
2215	98-99	-0.43057634921041554
2215	100	-0.3716587779628533
2216	1	-1.2132740643151543
2216	2	-1.2214594691141123
2216	3	-1.2866552484909555
2216	4	-1.461065263820693
2216	5	-1.4175269438675713
2216	6	-2.7784081040805333
2216	7	-2.614792024496751
2216	8	-3.4583207542556025
2216	9	-3.118841590053762
2216	10-11	-2.25687373465977
2216	12-13	-2.0069048128060345
2216	14-15	-2.2722998208595584
2216	16-17	-2.676950990059318
2216	18-19	-2.3074624766263554
2216	20-21	-2.1843239047654635
2216	22-23	-2.2783577756191136
2216	24-25	-2.5191193780972796
2216	26-27	-2.602596885393716
2216	28-29	-2.972008767947834
2216	30-31	-2.580215980783329
2216	32-33	-2.791821651743568
2216	34-35	-3.8030281268243016
2216	36-37	-2.7134639808580445
2216	38-39	-2.6645844285024864
2216	40-41	-3.1189453358103876
2216	42-43	-2.98186718185746
2216	44-45	-2.724555894769125
2216	46-47	-2.56290789294912
2216	48-49	-2.720729202975633
2216	50-51	-3.0593314062820696
2216	52-53	-3.461306882224065
2216	54-55	-2.8573786298557806
2216	56-57	-2.8937778623946997
2216	58-59	-3.868166659054296
2216	60-61	-4.5752215230263005
2216	62-63	-3.668721058414736
2216	64-65	-3.6418736105419782
2216	66-67	-3.3231292369107273
2216	68-69	-3.627628543061533
2216	70-71	-3.494089651193292
2216	72-73	-3.887630457650289
2216	74-75	-4.42563456632606
2216	76-77	-5.046671420988346
2216	78-79	-3.9876119420845555
2216	80-81	-3.8312993335390964
2216	82-83	-4.0131184305033045
2216	84-85	-4.076023902116351
2216	86-87	-3.705271565134993
2216	88-89	-3.7128902640769113
2216	90-91	-3.872398320162219
2216	92-93	-3.9143914648258935
2216	94-95	-3.8474792591727045
2216	96-97	-4.071396949429918
2216	98-99	-3.8729837317160225
2216	100	-3.456392576556638
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67774.0
3	12474.0
4	7853.0
5	6550.0
6	5861.0
7	6231.0
8	6604.0
9	7714.0
10	8793.0
11	9814.0
12	10906.0
13	12175.0
14	13763.0
15	14782.0
16	15081.0
17	16202.0
18	15397.0
19	13982.0
20	13960.0
21	14503.0
22	16056.0
23	19309.0
24	23401.0
25	28482.0
26	35383.0
27	42244.0
28	52037.0
29	64565.0
30	77919.0
31	93810.0
32	120236.0
33	160135.0
34	220384.0
35	321730.0
36	532543.0
37	948699.0
38	1252439.0
39	444702.0
40	976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37998051377648	20.15028850213544	14.231849731774012	23.237881252314068
2	15.217703592881415	19.985241794999954	40.87699566380023	23.920058948318395
3	18.251913520351415	25.176585114817556	30.283193195470716	26.28830816936031
4	12.506319422540516	16.64643113450879	39.06859792585619	31.778651517094502
5	13.029932154740347	38.080811345895896	35.684160066051575	13.205096433312185
6	31.06820791419267	37.8644081045423	17.64667854679358	13.420705434471452
7	27.208949816256663	32.447393633259075	22.583328844059526	17.760327706424732
8	24.890314370169563	36.72210241494294	20.430089354071633	17.957493860815866
9	24.83737206095104	17.045491035816173	21.615974128256784	36.501162774975995
10-11	23.87326626080626	27.121318487887248	28.973939485997942	20.03147576530855
12-13	24.147924521924125	24.48640018253158	29.191193907585994	22.174481387958302
14-15	21.594027093648094	26.7526747604525	26.205099896313683	25.44819824958572
16-17	21.29525627096545	30.149158762493713	26.860354552831083	21.695230413709755
18-19	21.252121693662136	29.154576969484687	28.742443686191404	20.850857650661773
20-21	22.20679695507128	28.325691646832297	28.43066514942035	21.03684624867608
22-23	21.92799670520001	28.021897871129504	28.49938613329891	21.55071929037157
24-25	21.72799788071367	28.291426798113683	28.380681031985972	21.599894289186675
26-27	21.521018045556094	28.26314995050868	28.45819993140612	21.757632072529102
28-29	21.55545513946253	28.3130075246581	28.545608799322803	21.585928536556565
30-31	21.816325084166387	28.541893699116077	28.170202167279886	21.471579049437647
32-33	21.559960664561874	28.38329659279477	28.489229758463086	21.56751298418027
34-35	21.724699974955882	28.154087974249876	28.518120227186376	21.60309182360787
36-37	21.90521930621836	28.38768083986995	28.09355017622807	21.61354967768362
38-39	21.75443814700807	28.561616076689983	28.17757679122127	21.506368985080677
40-41	21.708630823335433	28.273703300957337	28.336297010638077	21.68136886506915
42-43	21.642214886881934	28.318687826838367	28.308928028777462	21.730169257502236
44-45	21.73033698370851	28.367671787331844	28.229208916379367	21.672782312580274
46-47	21.714822883246853	28.418499887775443	28.134669465679092	21.73200776329861
48-49	21.70311247210096	28.362801671676902	28.08630131820894	21.8477845380132
50-51	21.74225370678794	28.304094345537617	28.08188141827575	21.87177052939869
52-53	21.72750846320815	28.31460424929627	28.145762586546535	21.812124700949038
54-55	21.79490415921796	28.51263661833478	28.001172428908294	21.691286793538968
56-57	21.717891654898597	28.34802713644588	28.033378715655633	21.90070249299989
58-59	21.661137821452257	28.055638909846287	28.2906082300101	21.992615038691355
60-61	21.832797862369212	28.360280102470742	28.005663056981874	21.801258978178176
62-63	21.691650181571063	28.442601817580154	28.00721362222889	21.858534378619897
64-65	21.754642750429266	28.22374218321993	28.108574490334902	21.913040576015906
66-67	21.655636679423555	28.257944326395524	28.006270922173687	22.080148072007237
68-69	21.877708866656928	28.364307426335987	27.95282638928717	21.80515731771991
70-71	21.879757682584636	28.236998633864484	28.031402315818337	21.85184136773254
72-73	21.729209977398412	28.340567763623664	28.089477740567915	21.840744518410013
74-75	21.815293200241957	28.399585926354586	27.958515743913555	21.826605129489902
76-77	21.89705568285255	28.337518396569084	27.85795598934409	21.907469931234274
78-79	21.870740812406893	28.378779454671893	27.97988924105449	21.77059049186672
80-81	21.855259288802063	28.33314884728611	27.95109373468484	21.860498129226986
82-83	21.788838451787292	28.35984291339007	28.03606460483626	21.81525402998638
84-85	21.8285857085716	28.275509366655587	27.939686433587912	21.9562184911849
86-87	21.919061065690865	28.40254104589736	27.87037687392629	21.808021014485483
88-89	21.78898292821782	28.284298037426314	27.952463488904872	21.974255545450994
90-91	21.761262082454643	28.22482739652294	27.981461933442013	22.032448587580408
92-93	21.871893419001914	28.387702153530004	27.84586584175169	21.89453858571639
94-95	21.824196164080988	28.20886372628692	27.948535573603884	22.018404536028203
96-97	21.86548998631392	28.244858112258786	27.90800657759559	21.981645323831707
98-99	21.86201620156314	28.34652702826267	27.817519236215038	21.973937533959152
100	21.889722010639286	28.1916321276731	27.867102498189727	22.051543363497892
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	962.0
1	843.0
2	890.5
3	1093.5
4	1061.0
5	884.5
6	714.5
7	620.5
8	601.5
9	590.5
10	544.5
11	530.0
12	569.0
13	653.0
14	751.0
15	867.5
16	1053.0
17	1323.0
18	1709.0
19	2393.0
20	3433.0
21	4966.5
22	7300.5
23	10738.0
24	15801.5
25	22516.5
26	31175.0
27	42139.5
28	54854.0
29	69725.5
30	84878.0
31	97812.0
32	109175.5
33	119937.0
34	130378.0
35	139630.5
36	148220.0
37	157245.0
38	165078.0
39	168996.5
40	171455.5
41	173186.0
42	172793.5
43	172102.5
44	170063.0
45	167282.0
46	164839.0
47	159927.0
48	153441.0
49	149208.0
50	145971.5
51	140737.5
52	132640.5
53	125600.5
54	119926.5
55	113222.0
56	104133.5
57	93226.5
58	83689.5
59	75900.5
60	66893.0
61	56260.0
62	47351.5
63	39595.0
64	32112.5
65	25535.5
66	19692.5
67	14741.5
68	11138.5
69	8500.5
70	6226.0
71	4444.5
72	3361.0
73	2403.5
74	1700.5
75	1173.5
76	763.0
77	537.0
78	380.0
79	274.0
80	195.0
81	122.5
82	88.0
83	66.0
84	52.0
85	43.0
86	35.0
87	31.5
88	29.0
89	23.0
90	19.0
91	20.0
92	17.0
93	11.0
94	10.5
95	11.0
96	8.5
97	8.5
98	12.0
99	18.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01778070978819627
2	0.038665652757942245
3	0.04046061752278391
4	0.04320585775136528
5	0.04641567709555273
6	0.04379714026213665
7	0.041474244684106265
8	0.04098854833597263
9	0.04149536191663381
10-11	0.04193882379971234
12-13	0.04311083020499131
14-15	0.04141089298652361
16-17	0.03775761175925764
18-19	0.040703465696850724
20-21	0.039985479790914054
22-23	0.041168044812456804
24-25	0.03801101854958822
26-27	0.03823274949112749
28-29	0.03868676999046979
30-31	0.03884514923442641
32-33	0.0385178321302494
34-35	0.03848615628145807
36-37	0.035656447122766506
38-39	0.036078791773317485
40-41	0.037303591259915336
42-43	0.037261356794860236
44-45	0.03608935038958126
46-47	0.03498069568188494
48-49	0.03504404737946759
50-51	0.03634275717991185
52-53	0.03682845352804548
54-55	0.03603655730826239
56-57	0.03408321329946411
58-59	0.03504404737946759
60-61	0.03442108901990489
62-63	0.034790640589137
64-65	0.03172864187264239
66-67	0.03285841381286626
68-69	0.03365031003264935
70-71	0.03543471618122725
72-73	0.03187646250033524
74-75	0.032277689918358665
76-77	0.03315405506825195
78-79	0.033470813556165185
80-81	0.033724220346495776
82-83	0.0320981934418745
84-85	0.03327019984715347
86-87	0.03360807556759426
88-89	0.03450555795001509
90-91	0.034674495810235476
92-93	0.03309070337066931
94-95	0.03454779241507019
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4735469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.2662086208165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31890023285808	48.143547722312384
2	13.490341883684595	16.799848841912873
3	4.207777860417156	7.860071222603626
4	1.755237685485	4.371679836141125
5	0.9157241497259789	2.8509335472978803
6	0.5352080539330608	1.9995225805042305
7	0.33957017120385236	1.480062298510981
8	0.2381439693600732	1.186265766237092
9	0.1822503161811535	1.0213232587690924
>10	0.9497700769097802	10.5659652483541
>50	0.05170523204425447	2.189135226411542
>100	0.015200543159255881	1.4490798810993792
>500	1.3586008492680714E-4	0.05978743242335046
>1k	3.39649528738775E-5	0.02277713742232019
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.1117232527549012E-5	0.0
5	0.0	0.0	0.0	4.2234465055098024E-5	0.0
6	0.0	0.0	0.0	4.2234465055098024E-5	0.0
7	0.0	0.0	0.0	1.2670339516529408E-4	0.0
8	0.0	0.0	0.0	1.2670339516529408E-4	0.0
9	0.0	2.1117232527549012E-5	0.0	2.3228955780303916E-4	0.0
10-11	0.0	2.1117232527549012E-5	0.0	2.5340679033058816E-4	0.0
12-13	0.0	2.1117232527549012E-5	0.0	3.0619987164946067E-4	0.0
14-15	0.0	2.1117232527549012E-5	0.0	3.378757204407842E-4	0.0
16-17	0.0	2.1117232527549012E-5	0.0	3.378757204407842E-4	0.0
18-19	0.0	4.2234465055098024E-5	0.0	3.589929529683332E-4	0.0
20-21	0.0	4.2234465055098024E-5	0.0	4.117860342872058E-4	0.0
22-23	0.0	5.279308131887253E-5	0.0	4.751377318698528E-4	0.0
24-25	0.0	6.335169758264704E-5	0.0	6.65192824617794E-4	0.0
26-27	0.0	6.335169758264704E-5	0.0	8.869237661570586E-4	0.0
28-29	0.0	8.446893011019606E-5	0.0	0.0023123369617666168	0.0
30-31	0.0	1.0558616263774507E-4	0.0	0.006493549002221322	0.0
32-33	0.0	1.0558616263774507E-4	0.0	0.016788199859401463	0.0
34-35	0.0	1.0558616263774507E-4	0.0	0.03275282765022852	0.0
36-37	0.0	1.0558616263774507E-4	0.0	0.058431382403728124	0.0
38-39	0.0	1.2670339516529408E-4	0.0	0.09358101594583346	0.0
40-41	0.0	1.2670339516529408E-4	0.0	0.1273580293736481	0.0
42-43	0.0	1.2670339516529408E-4	0.0	0.16192693902124583	0.0
44-45	0.0	1.583792439566176E-4	0.0	0.2061358653176697	0.0
46-47	0.0	1.9005509274794112E-4	0.0	0.25632096841938995	0.0
48-49	0.0	1.9005509274794112E-4	0.0	0.3089451118780421	0.0
50-51	0.0	1.9005509274794112E-4	0.0	0.36370209582197666	0.0
52-53	0.0	1.9005509274794112E-4	0.0	0.41576663261864877	0.0
54-55	0.0	1.9005509274794112E-4	0.0	0.47388125653446367	0.0
56-57	0.0	2.0061370901171564E-4	0.0	0.5396192013927237	0.0
58-59	0.0	2.4284817406681366E-4	0.0	0.6107314819292451	0.0
60-61	0.0	2.5340679033058816E-4	0.0	0.6847051474732493	0.0
62-63	0.0	2.850826391219117E-4	0.0	0.7580664132739545	0.0
64-65	0.0	2.956412553856862E-4	0.0	0.8357567117428073	0.0
66-67	0.0	2.956412553856862E-4	0.0	0.9244385297422494	0.0
68-69	0.0	3.167584879132352E-4	0.0	1.0234149985988716	0.0
70-71	0.0	3.695515692321077E-4	0.0	1.1261714520779251	0.0
72-73	0.0	3.8011018549588224E-4	0.0	1.229709243160498	0.0
74-75	0.0	3.8011018549588224E-4	0.0	1.3361189778668174	0.0
76-77	0.0	3.8011018549588224E-4	0.0	1.447258972659308	0.0
78-79	0.0	3.8011018549588224E-4	0.0	1.5722096375248154	0.0
80-81	0.0	3.8011018549588224E-4	0.0	1.7066419397951924	0.0
82-83	0.0	3.9066880175965676E-4	0.0	1.8459523227794334	0.0
84-85	0.0	4.012274180234313E-4	0.0	1.9864769466340082	0.0
86-87	0.0	4.012274180234313E-4	0.0	2.1348360637563037	0.0
88	0.0	4.012274180234313E-4	0.0	2.2528919522015665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4645	0.0	40.586857	1
GTATCAA	7740	0.0	35.716026	1
TCAACGC	8130	0.0	33.190315	4
ATCAACG	8175	0.0	33.007618	3
CAACGCA	8560	0.0	31.523048	5
AACGCAG	8910	0.0	30.279009	6
TATCAAC	9320	0.0	29.204697	2
ACGCAGA	10490	0.0	25.359951	7
GTACATG	13025	0.0	25.15833	1
CGCAGAG	10725	0.0	24.497513	8
TACATGG	13580	0.0	23.089586	2
ACATGGG	14095	0.0	21.578897	3
GCAGAGT	12755	0.0	20.009068	9
CATGGGG	10460	0.0	19.100613	4
GTGGTAT	2995	0.0	18.99395	1
AGAGTAC	10815	0.0	17.579214	10-11
GAGTACA	9705	0.0	17.197271	1
CAGAGTA	11855	0.0	16.88945	10-11
GTATAGG	1715	0.0	16.722164	1
ACCGCGT	290	1.1510656E-6	16.207256	8
>>END_MODULE