FastQCFastQC Report
Wed 25 May 2016
SRR2049382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735469
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101210.21372751041132354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91690.1936239050450969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54360.11479327601975643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67100.053.1094781
GTATCAA127350.043.672811
ATCAACG155200.035.247323
TCAACGC155600.035.1567084
CAACGCA160800.034.1074835
AACGCAG166050.033.082566
TATCAAC166100.033.019162
ACGCAGA193850.028.1683587
CGCAGAG198800.027.3013788
GTACATG124250.024.9731251
GCAGAGT218400.024.3128819
GTGGTAT34800.022.6963181
TACATGG131500.022.5153982
ACATGGG134900.021.3556773
GAGTACT148150.021.19848312-13
AGAGTAC199750.021.09678810-11
CAGAGTA212100.020.3562210-11
TGGTATC35550.020.226312
AGTACTT157050.019.33857712-13
GTACTTT164850.018.98672914-15
Produced by FastQC (version 0.11.5)