##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049382_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4735469
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90483835919948	33.0	31.0	34.0	30.0	34.0
2	32.08287732429459	33.0	31.0	34.0	30.0	34.0
3	32.01050793490571	34.0	31.0	34.0	30.0	34.0
4	35.73753581746602	37.0	35.0	37.0	33.0	37.0
5	35.80253761559837	37.0	35.0	37.0	35.0	37.0
6	35.8317847714767	37.0	35.0	37.0	35.0	37.0
7	35.8082567956838	37.0	35.0	37.0	35.0	37.0
8	35.80662696767733	37.0	35.0	37.0	35.0	37.0
9	37.55284344591845	39.0	37.0	39.0	35.0	39.0
10-11	37.51122813812106	39.0	37.0	39.0	35.0	39.0
12-13	37.45691609426648	39.0	37.0	39.0	34.5	39.0
14-15	38.76804451681555	40.0	38.0	41.0	35.0	41.0
16-17	38.794064326046694	40.0	38.0	41.0	35.0	41.0
18-19	38.85846702829224	40.0	38.0	41.0	35.0	41.0
20-21	38.887073909680325	40.0	38.0	41.0	35.0	41.0
22-23	38.858290593814466	40.0	38.0	41.0	35.0	41.0
24-25	38.83935308202841	40.0	38.0	41.0	35.0	41.0
26-27	38.730010691654826	40.0	38.0	41.0	34.5	41.0
28-29	38.65692933477128	40.0	38.0	41.0	34.0	41.0
30-31	38.56516154999642	40.0	38.0	41.0	34.0	41.0
32-33	38.444767350393384	40.0	38.0	41.0	34.0	41.0
34-35	38.35122318401831	40.0	38.0	41.0	34.0	41.0
36-37	38.25083492258106	40.0	38.0	41.0	34.0	41.0
38-39	38.168271505947985	40.0	38.0	41.0	34.0	41.0
40-41	38.06485154902292	40.0	38.0	41.0	33.0	41.0
42-43	37.94024308891052	40.0	38.0	41.0	33.0	41.0
44-45	37.78036452144444	40.0	37.0	41.0	33.0	41.0
46-47	37.57582269042412	40.0	37.0	41.0	33.0	41.0
48-49	37.39132354155417	40.0	37.0	41.0	32.5	41.0
50-51	37.255142415672026	40.0	37.0	41.0	32.0	41.0
52-53	37.12300175547554	40.0	36.5	41.0	32.0	41.0
54-55	36.983529931248626	40.0	36.0	41.0	31.0	41.0
56-57	36.842147103064136	39.5	36.0	41.0	31.0	41.0
58-59	36.65337931681107	39.0	35.5	41.0	31.0	41.0
60-61	36.47850487459637	39.0	35.0	41.0	31.0	41.0
62-63	36.2772503631636	39.0	35.0	41.0	31.0	41.0
64-65	36.05059245451718	38.5	35.0	40.5	30.5	41.0
66-67	35.780133076575936	38.0	35.0	40.0	30.0	41.0
68-69	35.48994154538864	37.0	35.0	40.0	30.0	41.0
70-71	35.16318753221698	37.0	34.5	39.5	29.0	41.0
72-73	34.86342841648842	36.0	34.0	39.0	29.0	41.0
74-75	34.55310012587982	36.0	34.0	39.0	29.0	41.0
76-77	34.224546396566	35.5	34.0	38.5	29.0	40.0
78-79	33.88994595889024	35.0	34.0	37.0	29.0	39.0
80-81	33.57199424175303	35.0	34.0	37.0	29.0	39.0
82-83	33.272735710021536	35.0	34.0	36.5	29.0	39.0
84-85	33.003304952476725	35.0	34.0	36.0	29.0	37.0
86-87	32.77146571965733	35.0	33.5	36.0	28.5	37.0
88-89	32.547976451751666	35.0	33.0	35.0	28.0	37.0
90-91	32.36217014618826	35.0	33.0	35.0	27.0	36.0
92-93	32.18213211827593	35.0	33.0	35.0	27.0	36.0
94-95	32.02939487091986	35.0	33.0	35.0	27.0	36.0
96-97	31.835070612857987	35.0	33.0	35.0	26.5	36.0
98-99	31.692268073130666	35.0	33.0	35.0	26.0	35.0
100	31.58394342777875	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14429754874794298
1101	2	0.15345151658557654
1101	3	0.3243686022879473
1101	4	0.18738632518883946
1101	5	0.2231573862944103
1101	6	0.18037197739545263
1101	7	0.06297602876536246
1101	8	0.14030555741037887
1101	9	0.22488227994418963
1101	10-11	0.24343573713001376
1101	12-13	0.13650650695885247
1101	14-15	0.2678319226864616
1101	16-17	0.20487751356436235
1101	18-19	0.34443501686729405
1101	20-21	0.2581027832903473
1101	22-23	0.27503919003135735
1101	24-25	0.29984386442325217
1101	26-27	0.3427846419009484
1101	28-29	0.3581563356203503
1101	30-31	0.3502874690145532
1101	32-33	0.3545066741699827
1101	34-35	0.32992728806095784
1101	36-37	0.37187435875143393
1101	38-39	0.34458763031401674
1101	40-41	0.4099264998267955
1101	42-43	0.42050397818366747
1101	44-45	0.3298017399731634
1101	46-47	0.2844908394384902
1101	48-49	0.364577526834303
1101	50-51	0.26473271051985137
1101	52-53	0.38083787067194663
1101	54-55	0.332315063843744
1101	56-57	0.3650340960208567
1101	58-59	0.352986467338404
1101	60-61	0.3253193855745806
1101	62-63	0.30891030535548936
1101	64-65	0.24965433959683736
1101	66-67	0.19870253891364342
1101	68-69	0.2944227376865527
1101	70-71	0.2867216755441717
1101	72-73	0.2135888585277428
1101	74-75	0.3030500117718091
1101	76-77	0.2548441266876793
1101	78-79	0.324642196129588
1101	80-81	0.3609915291660073
1101	82-83	0.3028562500088583
1101	84-85	0.3431092648501348
1101	86-87	0.2615766620160258
1101	88-89	0.3291719168774918
1101	90-91	0.40814667480646705
1101	92-93	0.36652466306045994
1101	94-95	0.44056576610029907
1101	96-97	0.42718550377439257
1101	98-99	0.42238667982685385
1101	100	0.48609546495038103
1102	1	0.24584706950778212
1102	2	0.22574646943484566
1102	3	0.34513645090513734
1102	4	0.2020593762216265
1102	5	0.1804853752842419
1102	6	0.19845127629682224
1102	7	0.16613200218353086
1102	8	0.2019371939081367
1102	9	0.21178110103466707
1102	10-11	0.22004917323182838
1102	12-13	0.21519808768464088
1102	14-15	0.3528100361618769
1102	16-17	0.27801988501578023
1102	18-19	0.3256376939915313
1102	20-21	0.2716383584502253
1102	22-23	0.2986392865614391
1102	24-25	0.2954767903057771
1102	26-27	0.3658311566304775
1102	28-29	0.35274035480819776
1102	30-31	0.3267129494819869
1102	32-33	0.3003467574413037
1102	34-35	0.35691187843583805
1102	36-37	0.355850136303026
1102	38-39	0.29906004256265106
1102	40-41	0.2866219778136312
1102	42-43	0.29313671031093946
1102	44-45	0.3742953436602505
1102	46-47	0.31498551703364797
1102	48-49	0.23565976073685846
1102	50-51	0.3428479236528119
1102	52-53	0.39575865474648
1102	54-55	0.2510254575101243
1102	56-57	0.3421357215232206
1102	58-59	0.2901102573998031
1102	60-61	0.2917718571053811
1102	62-63	0.34063708828399797
1102	64-65	0.2839866746127129
1102	66-67	0.30766256842005646
1102	68-69	0.32653741125890434
1102	70-71	0.3403864411502653
1102	72-73	0.33297034699388206
1102	74-75	0.31616875246688636
1102	76-77	0.3666937812950337
1102	78-79	0.38264489653921174
1102	80-81	0.3203442714762872
1102	82-83	0.3816301006947995
1102	84-85	0.34129563308157174
1102	86-87	0.3719646482342043
1102	88-89	0.31701413275994383
1102	90-91	0.3883951724429551
1102	92-93	0.39699716306371613
1102	94-95	0.3383157397087402
1102	96-97	0.4023188842866965
1102	98-99	0.43420159098832656
1102	100	0.46347664540647315
1103	1	0.14078299248951254
1103	2	0.17678449615632275
1103	3	0.2650800295699014
1103	4	0.1691265155131063
1103	5	0.1677641470190423
1103	6	0.08567131950424312
1103	7	0.11159690140118528
1103	8	0.061240841369176735
1103	9	0.14518945307023756
1103	10-11	0.1576878669415791
1103	12-13	0.14463020379932345
1103	14-15	0.18916939182603443
1103	16-17	0.14221645778671643
1103	18-19	0.23329169906735103
1103	20-21	0.29074641894060704
1103	22-23	0.21448518710048603
1103	24-25	0.2652796302495659
1103	26-27	0.23900228736437157
1103	28-29	0.24801900069615357
1103	30-31	0.25253084909815016
1103	32-33	0.2493926286160928
1103	34-35	0.2765414692238508
1103	36-37	0.29759175175701813
1103	38-39	0.26943307358390456
1103	40-41	0.2554258035577064
1103	42-43	0.2629348309980628
1103	44-45	0.2723332864838284
1103	46-47	0.2545755709380444
1103	48-49	0.30146098564615187
1103	50-51	0.2542832069130796
1103	52-53	0.24294807522175432
1103	54-55	0.27675757152736224
1103	56-57	0.22148593231668912
1103	58-59	0.2876568731684088
1103	60-61	0.27585097282155147
1103	62-63	0.21930992843753216
1103	64-65	0.26586189746650035
1103	66-67	0.19993263574811948
1103	68-69	0.1882084476076713
1103	70-71	0.3052027047574626
1103	72-73	0.25221830722627203
1103	74-75	0.1982537200613237
1103	76-77	0.20397548945620514
1103	78-79	0.15664137282756485
1103	80-81	0.23729175139322933
1103	82-83	0.17070848230437718
1103	84-85	0.16599852965643436
1103	86-87	0.16448189973912264
1103	88-89	0.21265637115582336
1103	90-91	0.25344687452152215
1103	92-93	0.25488870405832387
1103	94-95	0.21039483445044027
1103	96-97	0.28435047184464324
1103	98-99	0.2868620981511114
1103	100	0.2923226298434116
1104	1	0.15529691041753324
1104	2	0.17323419596372247
1104	3	0.2802316927111761
1104	4	0.14987377684701642
1104	5	0.14523176477888455
1104	6	0.1447653798520605
1104	7	0.1778198059795173
1104	8	0.08031811412882206
1104	9	0.150357568047923
1104	10-11	0.15433045104994392
1104	12-13	0.14280572871668085
1104	14-15	0.20834701366576525
1104	16-17	0.20091710863642476
1104	18-19	0.18024527070304686
1104	20-21	0.19518055004047596
1104	22-23	0.21705758484626614
1104	24-25	0.19518353402514776
1104	26-27	0.24901490349124344
1104	28-29	0.27419319074589765
1104	30-31	0.2283458458067642
1104	32-33	0.23676910503359494
1104	34-35	0.28593309720275073
1104	36-37	0.29849692833700914
1104	38-39	0.28030822581680326
1104	40-41	0.24430024155503816
1104	42-43	0.2937942537695619
1104	44-45	0.3155141579936611
1104	46-47	0.34906184530421314
1104	48-49	0.3315360414267232
1104	50-51	0.2993137247135138
1104	52-53	0.26716757955041004
1104	54-55	0.2699601651588708
1104	56-57	0.31026001105963275
1104	58-59	0.42676110781209076
1104	60-61	0.3495482508921768
1104	62-63	0.284023669536289
1104	64-65	0.25835054040873473
1104	66-67	0.32836361688598714
1104	68-69	0.31104218231697445
1104	70-71	0.3235214837605582
1104	72-73	0.31917090212956367
1104	74-75	0.34797786437760436
1104	76-77	0.2921369229737891
1104	78-79	0.3131845540653515
1104	80-81	0.3505272283489589
1104	82-83	0.3523170893517147
1104	84-85	0.3822250359355053
1104	86-87	0.3292784392576138
1104	88-89	0.31417791675345086
1104	90-91	0.3586949391160985
1104	92-93	0.3979707017609684
1104	94-95	0.40591562104560097
1104	96-97	0.43772519421354517
1104	98-99	0.4289225965491674
1104	100	0.42072643543703236
1105	1	0.1386766775414685
1105	2	0.12604408848245896
1105	3	0.2531405811286547
1105	4	0.12516068817497938
1105	5	0.12048497036036565
1105	6	0.12349905731399247
1105	7	0.1435603306577775
1105	8	0.1642744145106576
1105	9	0.14581495267056255
1105	10-11	0.18874552081848606
1105	12-13	0.18426149876334819
1105	14-15	0.18611583467176018
1105	16-17	0.19396356010486215
1105	18-19	0.17711364151340092
1105	20-21	0.16331636298536978
1105	22-23	0.17680882818761035
1105	24-25	0.1718488748538789
1105	26-27	0.18515736330361676
1105	28-29	0.17495245393356385
1105	30-31	0.2218868642859988
1105	32-33	0.22131958704933652
1105	34-35	0.23928027817668607
1105	36-37	0.17624432969466142
1105	38-39	0.17939410475896267
1105	40-41	0.23897296582788385
1105	42-43	0.280116585594385
1105	44-45	0.2868087061481006
1105	46-47	0.28381467965107277
1105	48-49	0.2423237953983488
1105	50-51	0.25812813097383014
1105	52-53	0.27170556922656175
1105	54-55	0.3044359288415137
1105	56-57	0.2291797772407449
1105	58-59	0.2976683639757667
1105	60-61	0.27079801518765834
1105	62-63	0.24231752236239146
1105	64-65	0.22136499498847684
1105	66-67	0.26928165653148994
1105	68-69	0.20460632930231526
1105	70-71	0.2773784641499475
1105	72-73	0.2814933871623282
1105	74-75	0.28613291670401253
1105	76-77	0.2686562448518899
1105	78-79	0.2814005654734473
1105	80-81	0.2702468872845145
1105	82-83	0.2850440138791157
1105	84-85	0.2802970908075224
1105	86-87	0.24440795889179867
1105	88-89	0.26535920888563425
1105	90-91	0.26030438851033466
1105	92-93	0.2933439398696791
1105	94-95	0.2849097225399717
1105	96-97	0.2912859234833327
1105	98-99	0.3182629774476773
1105	100	0.31866655470352256
1106	1	0.10424134056514589
1106	2	0.18249900651525053
1106	3	0.22962282156581182
1106	4	0.17161560620776584
1106	5	0.14375227454980433
1106	6	0.10413202634860141
1106	7	0.1493458497834652
1106	8	0.11514873145055304
1106	9	0.09160274866327711
1106	10-11	0.15837780679297708
1106	12-13	0.1380798047742786
1106	14-15	0.17387995124735056
1106	16-17	0.17481169307389877
1106	18-19	0.21990735736039824
1106	20-21	0.22065698229320674
1106	22-23	0.18463555860656555
1106	24-25	0.21142196228557708
1106	26-27	0.24741601539833624
1106	28-29	0.2496450768843843
1106	30-31	0.28862069853008165
1106	32-33	0.20285078012766178
1106	34-35	0.17910495941530513
1106	36-37	0.20438982149794782
1106	38-39	0.25339000639830545
1106	40-41	0.23373161792260788
1106	42-43	0.2639087076344566
1106	44-45	0.25301772254154287
1106	46-47	0.2644078035126398
1106	48-49	0.2620585403892406
1106	50-51	0.2301864187698186
1106	52-53	0.23409286430852205
1106	54-55	0.3219051911366009
1106	56-57	0.2510877918126937
1106	58-59	0.28829108710873896
1106	60-61	0.2214787984299207
1106	62-63	0.23867453511284253
1106	64-65	0.2118784285039652
1106	66-67	0.2518374397737446
1106	68-69	0.29692645680686525
1106	70-71	0.289449274714606
1106	72-73	0.34837066402936046
1106	74-75	0.29500245222313737
1106	76-77	0.2460572011360398
1106	78-79	0.3312662303186187
1106	80-81	0.3634925612371589
1106	82-83	0.3846182397443272
1106	84-85	0.3264582929933084
1106	86-87	0.36471305907394935
1106	88-89	0.3694325240040328
1106	90-91	0.3280662282188942
1106	92-93	0.42347599815747117
1106	94-95	0.41979246388787317
1106	96-97	0.43497672494052964
1106	98-99	0.39739321424184837
1106	100	0.3761369554321199
1107	1	0.12524915911394885
1107	2	0.10010394379231968
1107	3	0.18642511566319087
1107	4	0.14957407883836993
1107	5	0.10011676124216251
1107	6	0.12616012747506034
1107	7	0.11402408987154189
1107	8	0.07944913468538317
1107	9	0.1186480130036216
1107	10-11	0.12103718748514325
1107	12-13	0.0765045372563975
1107	14-15	0.1629353814913017
1107	16-17	0.10753491555121997
1107	18-19	0.12387688202663583
1107	20-21	0.11354431821332867
1107	22-23	0.10596952297331086
1107	24-25	0.13950636188636878
1107	26-27	0.17433563248188477
1107	28-29	0.2213300815611987
1107	30-31	0.2192546297787672
1107	32-33	0.2787552613600184
1107	34-35	0.22009074773715298
1107	36-37	0.15466041623574966
1107	38-39	0.1658133649282192
1107	40-41	0.19273559209051427
1107	42-43	0.23773005133285352
1107	44-45	0.22377737936677278
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2216	66-67	-0.46495840477555106
2216	68-69	-0.4039651218416651
2216	70-71	-0.5126878201377565
2216	72-73	-0.4790034684817073
2216	74-75	-0.46721297825237684
2216	76-77	-0.43946448893650825
2216	78-79	-0.5889241406319741
2216	80-81	-0.7293649664634287
2216	82-83	-0.6177658394896071
2216	84-85	-0.7549029471143598
2216	86-87	-0.589234753786748
2216	88-89	-0.8697799960189272
2216	90-91	-0.6998351324099019
2216	92-93	-0.817162633256352
2216	94-95	-0.7643224383752099
2216	96-97	-0.7631081164610514
2216	98-99	-0.8409321306296853
2216	100	-0.762429545663057
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	292.0
3	41.0
4	24.0
5	75.0
6	178.0
7	562.0
8	1321.0
9	2329.0
10	3266.0
11	4553.0
12	6383.0
13	8865.0
14	10944.0
15	14012.0
16	17302.0
17	23551.0
18	23418.0
19	13367.0
20	10541.0
21	10710.0
22	11463.0
23	12980.0
24	15170.0
25	18954.0
26	24360.0
27	32116.0
28	42333.0
29	55705.0
30	72811.0
31	94795.0
32	121817.0
33	160522.0
34	217343.0
35	314544.0
36	516549.0
37	952396.0
38	1364237.0
39	553799.0
40	1841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36126826871298	20.4740142273138	14.598365589108095	23.56635191486512
2	15.240729059782673	20.351711731192836	40.13509538337174	24.27246382565275
3	17.80949257613132	25.26689542260756	30.68950509442676	26.23410690683436
4	12.471214548096121	17.10419145743871	38.45411168702545	31.970482307439713
5	12.692301438358058	37.929780556054745	35.753375220067966	13.624542785519239
6	30.838698342233894	38.22782917594857	17.185942934057852	13.747529547759681
7	26.685002055762585	32.898705492528826	22.405700470217415	18.010591981491167
8	24.975155308359263	36.45633817331437	20.147387200031762	18.421119318294604
9	24.750748025169205	16.561675306078445	21.536768586173828	37.15080808257852
10-11	23.79783290736356	27.072967851758715	28.731726466797692	20.397472774080033
12-13	24.352670780999254	24.436976430333385	29.221282417692922	21.989070370974435
14-15	21.60850239268904	26.4864795805778	26.68285936740769	25.222158659325466
16-17	20.839722616674567	29.937650308517522	27.363723357044822	21.85890371776309
18-19	20.701829259536755	28.39793236909214	30.1200008109019	20.78023756046921
20-21	21.656424273389806	27.514380582014585	30.036561347559175	20.792633797036427
22-23	21.371684620889717	27.282672529373542	30.100482127535837	21.245160722200907
24-25	21.27280070320481	27.4741273951594	30.00316230991165	21.249909591724144
26-27	21.04426303314733	27.50841311432206	30.01856840216714	21.42875545036347
28-29	21.06279845362142	27.494625115960442	30.181655348138964	21.260921082279175
30-31	21.321958040894796	27.72048084793309	29.79992684989108	21.15763426128104
32-33	21.133602409564773	27.576565407253906	30.074389800697332	21.21544238248399
34-35	21.269956844814494	27.41764911092209	30.05607893454248	21.25631510972094
36-37	21.42363376448762	27.657739739052108	29.603672626551152	21.31495386990912
38-39	21.336351418084877	27.803503930510566	29.652999443453513	21.207145207951044
40-41	21.291092066162893	27.56205094157616	29.768656249947206	21.37820074231374
42-43	21.26699589299465	27.631476278208673	29.602695247997257	21.49883258079942
44-45	21.425856348364338	27.715145067338813	29.440026472635374	21.418972111661475
46-47	21.490884683199436	27.836842159167734	29.088525336537806	21.58374782109502
48-49	21.511356204474822	27.842197612930107	28.896653291602515	21.74979289099256
50-51	21.576100918065684	27.893320702349968	28.735902461552982	21.794675918031366
52-53	21.55850208576271	27.947932165936923	28.76658927854192	21.726976469758448
54-55	21.64666450073177	28.201728855289325	28.581270759002507	21.570335884976398
56-57	21.52562072862032	28.047851066943473	28.638154867453796	21.78837333698241
58-59	21.432829573967652	27.72878337953087	28.925963295661543	21.91242375083994
60-61	21.62984023931793	27.98900338678962	28.66315148397473	21.718004889917722
62-63	21.421218036653105	28.042979889446013	28.68663925342271	21.849162820478167
64-65	21.45111116226198	27.85522676106395	28.80680535543952	21.88685672123455
66-67	21.412286035785147	27.886701452179175	28.664502611764302	22.03650990027137
68-69	21.645567775809386	27.956683269984218	28.585975542926796	21.811773411279596
70-71	21.69109677596468	27.830263137749917	28.61229060189137	21.86634948439403
72-73	21.484292839917053	27.967392543850387	28.634239412765922	21.91407520346664
74-75	21.613790192154937	27.97775109237854	28.482267742262124	21.926190973204402
76-77	21.686259379816082	27.90930039358589	28.390924675541886	22.013515551056145
78-79	21.621490062414143	28.022545538249382	28.425824735682287	21.930139663654188
80-81	21.56130017576392	27.9366016595679	28.47057722916401	22.031520935504165
82-83	21.51977657642438	27.965578409426872	28.536807872618997	21.977837141529754
84-85	21.590047216464953	27.922282313217156	28.386601661535543	22.101068808782344
86-87	21.598458501917527	28.06049803464063	28.291070214122648	22.049973249319198
88-89	21.435991274177095	27.886036360411122	28.460130887343542	22.21784147806824
90-91	21.433540918587717	27.8257018765455	28.45612343063784	22.28463377422894
92-93	21.497041496342494	27.986880753687227	28.340308907525618	22.17576884244466
94-95	21.37126441733442	27.77072784836858	28.46224039506073	22.395767339236265
96-97	21.44047596672558	27.81280086600839	28.41301766375822	22.33370550350781
98-99	21.403542116083337	27.89491912347779	28.37537391994813	22.32616484049074
100	21.453046719719694	27.74995417482158	28.38371428064606	22.413284824812667
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	382.0
1	313.0
2	388.5
3	556.0
4	382.5
5	246.0
6	699.0
7	1965.5
8	3429.5
9	3870.0
10	2874.0
11	1866.5
12	1790.5
13	2048.5
14	2407.0
15	2782.5
16	3229.5
17	3720.5
18	4347.5
19	5166.5
20	6292.0
21	7818.5
22	9976.5
23	13113.5
24	17850.5
25	24370.5
26	32757.5
27	43136.5
28	55512.0
29	69981.0
30	84567.5
31	97204.5
32	107950.0
33	118669.0
34	129076.5
35	138156.0
36	146686.0
37	155255.5
38	162784.0
39	166820.5
40	169887.0
41	172350.5
42	172267.5
43	171833.0
44	170038.5
45	167006.5
46	164489.5
47	159181.5
48	152363.5
49	148464.0
50	145745.5
51	140865.5
52	132386.0
53	124865.0
54	118938.5
55	111788.5
56	102752.0
57	92191.5
58	82895.0
59	75608.5
60	66634.5
61	55537.5
62	46573.0
63	38576.5
64	30582.0
65	23989.5
66	18545.5
67	13727.5
68	10135.0
69	7623.5
70	5471.5
71	3829.0
72	2715.0
73	1876.5
74	1289.0
75	839.5
76	528.0
77	343.0
78	205.0
79	121.0
80	65.0
81	34.0
82	21.0
83	9.0
84	6.5
85	5.0
86	3.5
87	3.5
88	1.5
89	1.5
90	1.5
91	1.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040080507337288025
2	0.0
3	0.0
4	8.446893011019605E-5
5	0.0
6	0.0
7	0.0
8	0.0010769788589049998
9	0.0
10-11	0.0
12-13	0.044282836610270285
14-15	4.2234465055098024E-5
16-17	1.0558616263774506E-5
18-19	2.1117232527549012E-5
20-21	7.391031384642155E-5
22-23	0.0
24-25	1.372620114290686E-4
26-27	1.0558616263774507E-4
28-29	3.8011018549588224E-4
30-31	2.1117232527549012E-5
32-33	1.689378602203921E-4
34-35	2.1117232527549012E-5
36-37	5.2793081318872535E-5
38-39	3.273171041770097E-4
40-41	1.0558616263774507E-4
42-43	4.962549643974019E-4
44-45	2.745240228581372E-4
46-47	1.1614477890151958E-4
48-49	8.446893011019606E-4
50-51	4.962549643974019E-4
52-53	6.546342083540194E-4
54-55	4.962549643974019E-4
56-57	5.490480457162744E-4
58-59	1.478206276928431E-4
60-61	2.3228955780303916E-4
62-63	9.502754637397056E-4
64-65	4.645791156060783E-4
66-67	0.0014465304281371075
68-69	0.0014782062769284308
70-71	4.540204993423038E-4
72-73	0.0010347443938499017
74-75	9.608340800034801E-4
76-77	7.391031384642154E-4
78-79	0.0034632261345180384
80-81	0.0029564125538568617
82-83	0.0014570890444008818
84-85	0.003178143495396127
86-87	0.0015943510558299506
88-89	0.0020906060202273524
90-91	0.0030725573327583817
92-93	0.002871943623746666
94-95	0.004160094807927156
96-97	0.0038011018549588224
98-99	0.0034632261345180384
100	0.0017104958347314702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4735469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.027109097269246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9846367831865	45.61138081900714
2	14.317806655525764	17.189130842897054
3	4.394635307512997	7.9139175914038224
4	1.8556602250963894	4.455596751172966
5	0.9341396167620426	2.803685034372821
6	0.5742991491302921	2.068411060758777
7	0.3819598185348761	1.6049560588576322
8	0.2550432499248918	1.224760719021087
9	0.2006717081477632	1.084116826594904
>10	1.0219952560758396	10.969736268053875
>50	0.05943361878805761	2.424169346436816
>100	0.019014000693120293	1.8154117244070456
>500	5.284581389324253E-4	0.2260918750980901
>1k	7.046099300993402E-5	0.08573337811310212
>5k	7.046099300993383E-5	0.30886432840203604
>10k+	3.5230496504966914E-5	0.21403737540273923
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10121	0.21372751041132354	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9169	0.1936239050450969	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5436	0.11479327601975643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.2234465055098024E-5	0.0
5	0.0	2.1117232527549012E-5	0.0	8.446893011019605E-5	0.0
6	0.0	2.1117232527549012E-5	0.0	8.446893011019605E-5	0.0
7	0.0	2.1117232527549012E-5	0.0	1.2670339516529408E-4	0.0
8	0.0	2.1117232527549012E-5	0.0	1.2670339516529408E-4	0.0
9	0.0	2.1117232527549012E-5	0.0	1.9005509274794112E-4	0.0
10-11	0.0	2.1117232527549012E-5	0.0	1.9005509274794112E-4	0.0
12-13	0.0	2.1117232527549012E-5	0.0	2.1117232527549014E-4	0.0
14-15	0.0	2.1117232527549012E-5	0.0	2.3228955780303916E-4	0.0
16-17	0.0	2.1117232527549012E-5	0.0	2.3228955780303916E-4	0.0
18-19	0.0	2.1117232527549012E-5	0.0	2.3228955780303916E-4	0.0
20-21	0.0	2.1117232527549012E-5	0.0	2.7452402285813715E-4	0.0
22-23	0.0	2.1117232527549012E-5	0.0	3.3787572044078425E-4	0.0
24-25	0.0	4.2234465055098024E-5	0.0	5.384894294524999E-4	0.0
26-27	0.0	4.2234465055098024E-5	0.0	7.391031384642154E-4	0.0
28-29	0.0	6.335169758264704E-5	0.0	0.002175074950337548	0.0
30-31	0.0	6.335169758264704E-5	0.0	0.006366845607056027	0.0
32-33	0.0	6.335169758264704E-5	0.0	0.016650937847972398	0.0
34-35	0.0	8.446893011019605E-5	0.0	0.032573331173744356	0.0
36-37	0.0	8.446893011019605E-5	0.0	0.05809350668328734	0.0
38-39	0.0	1.0558616263774507E-4	0.0	0.09338040223682173	0.0
40-41	0.0	1.0558616263774507E-4	0.0	0.12724188459474658	0.0
42-43	0.0	1.0558616263774507E-4	0.0	0.16186358732366318	0.0
44-45	0.0	1.0558616263774507E-4	0.0	0.20630480317789007	0.0
46-47	0.0	1.2670339516529408E-4	0.0	0.2569439267789526	0.0
48-49	0.0	1.2670339516529408E-4	0.0	0.30972644948156136	0.0
50-51	0.0	1.2670339516529408E-4	0.0	0.36483186776220056	0.0
52-53	0.0	1.2670339516529408E-4	0.0	0.41697031487271907	0.0
54-55	0.0	1.372620114290686E-4	0.0	0.4749265595445773	0.0
56-57	0.0	1.478206276928431E-4	0.0	0.5409707042744869	0.0
58-59	0.0	1.478206276928431E-4	0.0	0.6124103019151852	0.0
60-61	0.0	1.478206276928431E-4	0.0	0.6865634639356735	0.0
62-63	0.0	1.478206276928431E-4	0.0	0.7599564055851702	0.0
64-65	0.0	1.689378602203921E-4	0.0	0.8380162556232551	0.0
66-67	0.0	1.689378602203921E-4	0.0	0.926983156261819	0.0
68-69	0.0	2.1117232527549014E-4	0.0	1.026434762850311	0.0
70-71	0.0	2.4284817406681366E-4	0.0	1.129265126643211	0.0
72-73	0.0	2.5340679033058816E-4	0.0	1.2335209036317205	0.0
74-75	0.0	2.5340679033058816E-4	0.0	1.3407647690228781	0.0
76-77	0.0	2.5340679033058816E-4	0.0	1.4526755428026243	0.0
78-79	0.0	2.850826391219117E-4	0.0	1.5783653108065958	0.0
80-81	0.0	2.956412553856862E-4	0.0	1.7137478885407127	0.0
82-83	0.0	2.956412553856862E-4	0.0	1.8535650851056147	0.0
84-85	0.0	2.956412553856862E-4	0.0	1.9947865776335987	0.0
86-87	0.0	2.956412553856862E-4	0.0	2.1436102738715004	0.0
88	0.0	2.956412553856862E-4	0.0	2.262711465326877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6710	0.0	53.109478	1
GTATCAA	12735	0.0	43.67281	1
ATCAACG	15520	0.0	35.24732	3
TCAACGC	15560	0.0	35.156708	4
CAACGCA	16080	0.0	34.107483	5
AACGCAG	16605	0.0	33.08256	6
TATCAAC	16610	0.0	33.01916	2
ACGCAGA	19385	0.0	28.168358	7
CGCAGAG	19880	0.0	27.301378	8
GTACATG	12425	0.0	24.973125	1
GCAGAGT	21840	0.0	24.312881	9
GTGGTAT	3480	0.0	22.696318	1
TACATGG	13150	0.0	22.515398	2
ACATGGG	13490	0.0	21.355677	3
GAGTACT	14815	0.0	21.198483	12-13
AGAGTAC	19975	0.0	21.096788	10-11
CAGAGTA	21210	0.0	20.35622	10-11
TGGTATC	3555	0.0	20.22631	2
AGTACTT	15705	0.0	19.338577	12-13
GTACTTT	16485	0.0	18.986729	14-15
>>END_MODULE