FastQCFastQC Report
Wed 25 May 2016
SRR2049381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9327359
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81450.041.8458141
GTATCAA132150.034.4004751
TCAACGC140500.031.0832084
ATCAACG142600.030.6584283
CAACGCA148150.029.5733645
AACGCAG157550.027.9840356
TATCAAC165800.026.6522
GTACATG250400.026.4909671
TACATGG251650.024.9198462
ACATGGG258400.023.4320263
ACGCAGA187250.023.2437277
CGCAGAG193900.021.9617528
CATGGGG189400.020.3774414
GAGTACA180400.020.0138111
GCAGAGT225100.018.1869249
AGTACAT193800.017.270812
AGAGTAC190000.016.6609810-11
CAGAGTA211800.016.04460110-11
GTGGTAT57150.015.6294061
GTATAGA41650.015.5764751
Produced by FastQC (version 0.11.5)