##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049381_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9327359 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.926698865134277 31.0 31.0 34.0 28.0 34.0 2 31.019083536936876 31.0 31.0 34.0 28.0 34.0 3 31.20403664102561 31.0 31.0 34.0 28.0 34.0 4 34.802493074406165 37.0 35.0 37.0 32.0 37.0 5 34.84352676893856 37.0 35.0 37.0 32.0 37.0 6 34.79120434841202 37.0 35.0 37.0 32.0 37.0 7 34.76559334748453 37.0 35.0 37.0 32.0 37.0 8 34.75776894617222 37.0 35.0 37.0 32.0 37.0 9 36.3676520867268 39.0 37.0 39.0 32.0 39.0 10-11 36.33113221009291 39.0 37.0 39.0 32.0 39.0 12-13 36.27357985256062 39.0 37.0 39.0 32.0 39.0 14-15 37.489884221246335 40.0 38.0 41.0 32.0 41.0 16-17 37.45061956980534 40.0 38.0 41.0 32.0 41.0 18-19 37.46481410225553 40.0 38.0 41.0 32.0 41.0 20-21 37.54136733667054 40.0 38.0 41.0 32.0 41.0 22-23 37.48367860613063 40.0 38.0 41.0 32.0 41.0 24-25 37.490717629717054 40.0 38.0 41.0 32.0 41.0 26-27 37.48107272380103 40.0 38.0 41.0 32.0 41.0 28-29 37.415835125462635 40.0 38.0 41.0 32.0 41.0 30-31 37.34948242047936 40.0 37.5 41.0 32.0 41.0 32-33 37.30509375697879 40.0 37.0 41.0 31.5 41.0 34-35 37.20313370590753 40.0 37.0 41.0 31.0 41.0 36-37 37.12140762460199 40.0 37.0 41.0 31.0 41.0 38-39 37.02373780187939 40.0 37.0 41.0 31.0 41.0 40-41 36.92215770830736 40.0 37.0 41.0 30.0 41.0 42-43 36.77556953688605 40.0 37.0 41.0 30.0 41.0 44-45 36.64753549209374 40.0 36.5 41.0 30.0 41.0 46-47 36.53246218999397 40.0 36.0 41.0 30.0 41.0 48-49 36.40525962386566 40.0 36.0 41.0 30.0 41.0 50-51 36.2623886890169 39.5 36.0 41.0 29.5 41.0 52-53 36.11886933911303 39.0 35.0 41.0 29.0 41.0 54-55 35.97550909105139 39.0 35.0 41.0 29.0 41.0 56-57 35.847246149740776 39.0 35.0 41.0 29.0 41.0 58-59 35.63260522083475 39.0 35.0 40.5 28.0 41.0 60-61 35.407205029848214 38.5 35.0 40.0 28.0 41.0 62-63 35.18904885080546 38.0 34.5 40.0 28.0 41.0 64-65 34.93327167958262 38.0 34.0 40.0 28.0 41.0 66-67 34.66013637944031 37.0 34.0 40.0 27.5 41.0 68-69 34.35946670434793 37.0 34.0 40.0 26.5 41.0 70-71 34.0629424684951 36.0 34.0 39.0 26.0 41.0 72-73 33.70920562830271 36.0 34.0 39.0 26.0 41.0 74-75 33.35255960449254 35.0 33.0 39.0 26.0 40.0 76-77 33.009993986507865 35.0 33.0 37.5 26.0 40.0 78-79 32.685425424281405 35.0 33.0 37.0 25.0 39.0 80-81 32.321019915712476 35.0 33.0 37.0 24.5 39.0 82-83 31.994742402431385 35.0 33.0 36.0 24.0 38.5 84-85 31.694769441167644 35.0 32.0 36.0 23.5 37.0 86-87 31.370395789419064 35.0 32.0 35.5 22.0 37.0 88-89 31.109797478578876 35.0 32.0 35.0 20.0 36.5 90-91 30.891327116282326 35.0 32.0 35.0 19.5 36.0 92-93 30.635574442883566 35.0 32.0 35.0 18.0 36.0 94-95 30.417829044641685 35.0 32.0 35.0 11.0 36.0 96-97 30.148021749779332 34.5 31.5 35.0 2.0 35.5 98-99 29.912349144061036 34.0 31.0 35.0 2.0 35.0 100 29.76927949272672 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3642334298415548 1101 2 0.33388126264549456 1101 3 0.32417729763967174 1101 4 0.3333355522687995 1101 5 0.37475398464997767 1101 6 0.4345159670112295 1101 7 0.39412452298799394 1101 8 0.4505536640053407 1101 9 0.5584234931840584 1101 10-11 0.5836231303664903 1101 12-13 0.5881060372801628 1101 14-15 0.6415331646736533 1101 16-17 0.6897774843011177 1101 18-19 0.6460427406762861 1101 20-21 0.6122085978392988 1101 22-23 0.6648972810686331 1101 24-25 0.6584260076514497 1101 26-27 0.5741612145346551 1101 28-29 0.6266798390582906 1101 30-31 0.6225666617813559 1101 32-33 0.6260842989618496 1101 34-35 0.6650844145146735 1101 36-37 0.6467729729014877 1101 38-39 0.6730542200046656 1101 40-41 0.7038395072613568 1101 42-43 0.6333505797511947 1101 44-45 0.6026439673081185 1101 46-47 0.5780153911361339 1101 48-49 0.4690143645850853 1101 50-51 0.5219663293806107 1101 52-53 0.5131919881165103 1101 54-55 0.5392240761813412 1101 56-57 0.4964213695138042 1101 58-59 0.6036462128421789 1101 60-61 0.6071240078602713 1101 62-63 0.6191259234265303 1101 64-65 0.5679290717917382 1101 66-67 0.5860505353919621 1101 68-69 0.5913122947244958 1101 70-71 0.5788900211266395 1101 72-73 0.6725027909073518 1101 74-75 0.6546124706775203 1101 76-77 0.6608403171741841 1101 78-79 0.6248571801912703 1101 80-81 0.6544604097651927 1101 82-83 0.6905366946772276 1101 84-85 0.6159986838501439 1101 86-87 0.6795681152515662 1101 88-89 0.6788206307925435 1101 90-91 0.6532404667391134 1101 92-93 0.6420175053206769 1101 94-95 0.6903784546574094 1101 96-97 0.7434207663513419 1101 98-99 0.7589284659969806 1101 100 0.7108404327521853 1102 1 0.2722177488147999 1102 2 0.2841731780823018 1102 3 0.3184101986836403 1102 4 0.3226033044510572 1102 5 0.25835672872332793 1102 6 0.39085264425108335 1102 7 0.405268887002471 1102 8 0.41854568510716206 1102 9 0.5328014620216663 1102 10-11 0.47143384440443015 1102 12-13 0.5334895820945107 1102 14-15 0.5855969344384775 1102 16-17 0.5740227843781511 1102 18-19 0.6054076716952537 1102 20-21 0.5021133163096749 1102 22-23 0.5266403635343266 1102 24-25 0.5808472360628727 1102 26-27 0.5614406771193856 1102 28-29 0.615834766972867 1102 30-31 0.5766005907046861 1102 32-33 0.6217190707746667 1102 34-35 0.6356882741187846 1102 36-37 0.6227609700157402 1102 38-39 0.6362949707192058 1102 40-41 0.6778039896653922 1102 42-43 0.6131779318549206 1102 44-45 0.5877715321947363 1102 46-47 0.5001738196685679 1102 48-49 0.5302447372413113 1102 50-51 0.626056698389398 1102 52-53 0.6362349838210051 1102 54-55 0.6096830485835625 1102 56-57 0.5370963394267747 1102 58-59 0.6376535039969298 1102 60-61 0.604471897763311 1102 62-63 0.609684532888565 1102 64-65 0.5855921567565474 1102 66-67 0.6202696481467882 1102 68-69 0.6433934259186245 1102 70-71 0.6529355990682575 1102 72-73 0.6367306316758246 1102 74-75 0.5650003764604747 1102 76-77 0.6311054366119606 1102 78-79 0.6096989124596206 1102 80-81 0.5502482324747291 1102 82-83 0.5833928789762268 1102 84-85 0.6066880764931852 1102 86-87 0.6034955681445702 1102 88-89 0.6079523646991589 1102 90-91 0.6857294413001114 1102 92-93 0.636274052824195 1102 94-95 0.626326654989871 1102 96-97 0.6323346547200366 1102 98-99 0.7137694127641581 1102 100 0.6921219403379126 1103 1 0.19203935425070284 1103 2 0.2246123697870388 1103 3 0.2530861337301964 1103 4 0.21228158300588262 1103 5 0.21142768050210492 1103 6 0.30499207570832 1103 7 0.3243875090622197 1103 8 0.3181454372751844 1103 9 0.4180003026084478 1103 10-11 0.383191408805132 1103 12-13 0.40528047159165936 1103 14-15 0.44404051497358665 1103 16-17 0.43014705187705005 1103 18-19 0.3894614497824378 1103 20-21 0.4094578982907535 1103 22-23 0.40370800785386507 1103 24-25 0.40957990571988745 1103 26-27 0.4166088485281705 1103 28-29 0.43628323135732927 1103 30-31 0.415841610178866 1103 32-33 0.46072021393679563 1103 34-35 0.4667071931611275 1103 36-37 0.44033180549731554 1103 38-39 0.47907241298796066 1103 40-41 0.4345647734746052 1103 42-43 0.4083970396563643 1103 44-45 0.33807414104722255 1103 46-47 0.3799438149423935 1103 48-49 0.4048030137183929 1103 50-51 0.3389348881909342 1103 52-53 0.39436478072098424 1103 54-55 0.3233203317816873 1103 56-57 0.27789723301778935 1103 58-59 0.3569618986859808 1103 60-61 0.3317008983426959 1103 62-63 0.34345225554387326 1103 64-65 0.29390157655411997 1103 66-67 0.39623896768170397 1103 68-69 0.3862115419775378 1103 70-71 0.32651416297306923 1103 72-73 0.33583780656580586 1103 74-75 0.361227811879246 1103 76-77 0.4002136757121164 1103 78-79 0.2831646833316057 1103 80-81 0.2345853507605966 1103 82-83 0.2822741683474703 1103 84-85 0.340135657894173 1103 86-87 0.3739137273016979 1103 88-89 0.338833915400798 1103 90-91 0.3638698071661217 1103 92-93 0.34243848446714864 1103 94-95 0.3715740236755245 1103 96-97 0.3462120186645379 1103 98-99 0.36071225022373454 1103 100 0.3567061143233765 1104 1 0.14573934723140525 1104 2 0.13677593699462776 1104 3 0.1674300705916032 1104 4 0.18222291702359428 1104 5 0.24422913744528785 1104 6 0.2551877386591457 1104 7 0.31465283855249027 1104 8 0.2914627249304331 1104 9 0.34079136221545525 1104 10-11 0.35370267697305025 1104 12-13 0.3382749592486576 1104 14-15 0.44106770488510705 1104 16-17 0.4340148773860619 1104 18-19 0.4291520193546603 1104 20-21 0.41849261310980523 1104 22-23 0.4419433616484554 1104 24-25 0.4485605116169822 1104 26-27 0.39639107478649294 1104 28-29 0.4017452657255731 1104 30-31 0.3707580805245243 1104 32-33 0.4035395262210031 1104 34-35 0.4244286410669673 1104 36-37 0.38759850155729225 1104 38-39 0.4400301140360341 1104 40-41 0.43719827176146 1104 42-43 0.4020186358448825 1104 44-45 0.37192344767212404 1104 46-47 0.31753218681606654 1104 48-49 0.3224668320852615 1104 50-51 0.3684432795431576 1104 52-53 0.3660646684122 1104 54-55 0.45473402402053864 1104 56-57 0.4024187416844356 1104 58-59 0.5040396494833317 1104 60-61 0.44466633816755774 1104 62-63 0.4254220901077801 1104 64-65 0.46772833916582357 1104 66-67 0.4467804133501403 1104 68-69 0.4451153304246489 1104 70-71 0.4281026357491555 1104 72-73 0.45053723251879063 1104 74-75 0.4809624056154007 1104 76-77 0.5282719238267717 1104 78-79 0.4217391021556125 1104 80-81 0.50064728839412 1104 82-83 0.43903484974589446 1104 84-85 0.4617840798680888 1104 86-87 0.4877782679982765 1104 88-89 0.3999815488003762 1104 90-91 0.42377516179425356 1104 92-93 0.40162655451097606 1104 94-95 0.42669342304290936 1104 96-97 0.44038122982697914 1104 98-99 0.4636688007648786 1104 100 0.4585968830681928 1105 1 0.1650896806437423 1105 2 0.13871186332958274 1105 3 0.1700700541857323 1105 4 0.19220013622854992 1105 5 0.16967599150196122 1105 6 0.2107454794228545 1105 7 0.19655402007773404 1105 8 0.29345044490104755 1105 9 0.3323032783236357 1105 10-11 0.2997610657350265 1105 12-13 0.30190135000759 1105 14-15 0.34302719574841234 1105 16-17 0.34595585281255126 1105 18-19 0.3209121246816906 1105 20-21 0.32392277133568825 1105 22-23 0.3212224719829777 1105 24-25 0.33043262357023906 1105 26-27 0.2894683773655373 1105 28-29 0.34490066848464807 1105 30-31 0.3029694355384791 1105 32-33 0.31987250739964423 1105 34-35 0.38343220295625713 1105 36-37 0.38220231911594027 1105 38-39 0.36435249989672513 1105 40-41 0.39598644356588864 1105 42-43 0.4161678796788095 1105 44-45 0.35732295835359906 1105 46-47 0.3391984101376124 1105 48-49 0.33004356861135165 1105 50-51 0.36711035509588896 1105 52-53 0.3670432424462149 1105 54-55 0.40975572691964146 1105 56-57 0.35338591691627386 1105 58-59 0.3439164520699478 1105 60-61 0.3440148204564508 1105 62-63 0.2707338100515031 1105 64-65 0.2960714870254151 1105 66-67 0.40641296849722863 1105 68-69 0.32761568972329513 1105 70-71 0.34185025303180083 1105 72-73 0.3082152660499631 1105 74-75 0.3331160753363136 1105 76-77 0.4080975883401692 1105 78-79 0.3973853249392647 1105 80-81 0.41002928000965255 1105 82-83 0.41370332845440316 1105 84-85 0.3708715306378778 1105 86-87 0.41261282252678555 1105 88-89 0.3629101647735773 1105 90-91 0.3724260584019703 1105 92-93 0.37686832468086706 1105 94-95 0.36720404105339455 1105 96-97 0.3243290058733983 1105 98-99 0.35030965676133974 1105 100 0.3292801934568388 1106 1 0.08142257978509448 1106 2 0.0818874407859802 1106 3 0.11508952238016334 1106 4 0.10927637994141293 1106 5 0.12317771745885864 1106 6 0.15054106389033706 1106 7 0.15080116730041482 1106 8 0.15207388326258808 1106 9 0.25771535249289457 1106 10-11 0.21673444182220436 1106 12-13 0.2311474090017427 1106 14-15 0.33140144891723367 1106 16-17 0.29065957694839994 1106 18-19 0.27938778283191823 1106 20-21 0.29887885637769784 1106 22-23 0.296233253487415 1106 24-25 0.3075012411323641 1106 26-27 0.23101105724013848 1106 28-29 0.32110934119970835 1106 30-31 0.28230990064714945 1106 32-33 0.3316350208365648 1106 34-35 0.3364787053124303 1106 36-37 0.24710804269303566 1106 38-39 0.3173379026497969 1106 40-41 0.3241992199102768 1106 42-43 0.3309204297474011 1106 44-45 0.2825418134264339 1106 46-47 0.25694453027209363 1106 48-49 0.29709616244118564 1106 50-51 0.31755786947811515 1106 52-53 0.35122113781918074 1106 54-55 0.35670822082789755 1106 56-57 0.3159297224406217 1106 58-59 0.35422873558221113 1106 60-61 0.34947504013567965 1106 62-63 0.2961186998855183 1106 64-65 0.3187370831358223 1106 66-67 0.35707734982293005 1106 68-69 0.34658419995290757 1106 70-71 0.2889419618054774 1106 72-73 0.3390807709405621 1106 74-75 0.4020575050654003 1106 76-77 0.47966269223739033 1106 78-79 0.3158407125173994 1106 80-81 0.3857553096336517 1106 82-83 0.3766349769941755 1106 84-85 0.39717642148499266 1106 86-87 0.4110904885553346 1106 88-89 0.42918012073666034 1106 90-91 0.4604480635024153 1106 92-93 0.5008836152993688 1106 94-95 0.4831983545351157 1106 96-97 0.43983455306991814 1106 98-99 0.46166283848703316 1106 100 0.5042742005650815 1107 1 0.12289145706078841 1107 2 0.10608868925417525 1107 3 0.12186316679943232 1107 4 0.13200251700743593 1107 5 0.1577866535403203 1107 6 0.19237573866404034 1107 7 0.18289375169991473 1107 8 0.28411181896899507 1107 9 0.29712218167566107 1107 10-11 0.2533092836789166 1107 12-13 0.24136816475212441 1107 14-15 0.29067015699786936 1107 16-17 0.29058232502441683 1107 18-19 0.2562596911053916 1107 20-21 0.2692821541244399 1107 22-23 0.2736773449652006 1107 24-25 0.30084976892569415 1107 26-27 0.268292865408462 1107 28-29 0.33805624107406373 1107 30-31 0.366153934439545 1107 32-33 0.3438977332485891 1107 34-35 0.3391773700522194 1107 36-37 0.3417985579924263 1107 38-39 0.36238466349145426 1107 40-41 0.36336772468342105 1107 42-43 0.3382174448309172 1107 44-45 0.2770775149805118 1107 46-47 0.3079026127850426 1107 48-49 0.2824000663204558 1107 50-51 0.36969267969030994 1107 52-53 0.4104113766278559 1107 54-55 0.41631484898960025 1107 56-57 0.37758595831823527 1107 58-59 0.380654424287556 1107 60-61 0.3734572475200011 1107 62-63 0.33208761827821576 1107 64-65 0.3779911252130006 1107 66-67 0.3431766519667079 1107 68-69 0.3776196043762212 1107 70-71 0.362381770234947 1107 72-73 0.3780262163435708 1107 74-75 0.4031915292058983 1107 76-77 0.4800220174772676 1107 78-79 0.3927618099525958 1107 80-81 0.3179005484107478 1107 82-83 0.3639105026979621 1107 84-85 0.3811007112781013 1107 86-87 0.4136616431376794 1107 88-89 0.38134029508343303 1107 90-91 0.39922847151297347 1107 92-93 0.35846049289996884 1107 94-95 0.3229420703135162 1107 96-97 0.30683664729395943 1107 98-99 0.302812902558685 1107 100 0.3286268420456011 1108 1 -0.02448321884461535 1108 2 0.06114492869824062 1108 3 0.11246857733942761 1108 4 0.046730072108978504 1108 5 0.08621935590219465 1108 6 0.1634880278865225 1108 7 0.12731690070955182 1108 8 0.12465589516488507 1108 9 0.20704375821797782 1108 10-11 0.15305252504332145 1108 12-13 0.208964044695108 1108 14-15 0.2472259495724387 1108 16-17 0.21506240602835192 1108 18-19 0.19897415427215748 1108 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2215 44-45 -0.38509871827181286 2215 46-47 -0.45028545338896464 2215 48-49 -0.3825628292257477 2215 50-51 -0.28448851123392416 2215 52-53 -0.3254612076693135 2215 54-55 -0.42530888003637557 2215 56-57 -0.36022270629393915 2215 58-59 -0.34003900646334984 2215 60-61 -0.21767612466329211 2215 62-63 -0.2730761255779228 2215 64-65 -0.21503816533481057 2215 66-67 -0.20108393294889026 2215 68-69 -0.22949566222013829 2215 70-71 -0.25958419269316835 2215 72-73 -0.33609077264927834 2215 74-75 -0.2835722602878832 2215 76-77 -0.2641060084180751 2215 78-79 -0.348268524961199 2215 80-81 -0.3046933266873708 2215 82-83 -0.2809919773192959 2215 84-85 -0.312031697406546 2215 86-87 -0.18448242189321107 2215 88-89 -0.20394765659149527 2215 90-91 -0.23453968997916164 2215 92-93 -0.20787306894503033 2215 94-95 -0.2641691161702582 2215 96-97 -0.20441710904201216 2215 98-99 -0.306531876309478 2215 100 -0.23975059302980384 2216 1 -1.074109570966204 2216 2 -1.001246791416321 2216 3 -1.0802537204092104 2216 4 -1.2902581607795085 2216 5 -1.2652925762655727 2216 6 -2.6445304606909588 2216 7 -2.5820590758991457 2216 8 -3.478516968365941 2216 9 -2.8750722270356874 2216 10-11 -2.0355876281410303 2216 12-13 -1.8299307871619632 2216 14-15 -2.0978061186470853 2216 16-17 -2.455694966837882 2216 18-19 -2.0775547907490832 2216 20-21 -1.8707922483342827 2216 22-23 -2.0292207608283093 2216 24-25 -2.2151522609385026 2216 26-27 -2.2669088271548645 2216 28-29 -2.699130085481876 2216 30-31 -2.274396272923532 2216 32-33 -2.4856278336063085 2216 34-35 -3.538787537229922 2216 36-37 -2.5211904215904255 2216 38-39 -2.3221949216563047 2216 40-41 -2.8935592893146946 2216 42-43 -2.6982463892286646 2216 44-45 -2.478709152144816 2216 46-47 -2.3043779543419305 2216 48-49 -2.5155362401406265 2216 50-51 -2.86709046231946 2216 52-53 -3.243644434565333 2216 54-55 -2.5854671930752033 2216 56-57 -2.572160285705934 2216 58-59 -3.7005552933835304 2216 60-61 -4.452775540675216 2216 62-63 -3.4739455731859863 2216 64-65 -3.484463622602192 2216 66-67 -3.121178358289356 2216 68-69 -3.4920627445412187 2216 70-71 -3.220454723657177 2216 72-73 -3.7177517249240566 2216 74-75 -4.227442666215644 2216 76-77 -4.933515513380492 2216 78-79 -3.8370662835574407 2216 80-81 -3.6540378833028555 2216 82-83 -3.932542696784786 2216 84-85 -3.972022403651742 2216 86-87 -3.6037898319504116 2216 88-89 -3.5537611739201225 2216 90-91 -3.701279642353388 2216 92-93 -3.718023447448747 2216 94-95 -3.6048764560785855 2216 96-97 -3.9505565929372857 2216 98-99 -3.675771804077666 2216 100 -3.2402569249967144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 128313.0 3 22206.0 4 14745.0 5 12374.0 6 11529.0 7 12252.0 8 13506.0 9 15495.0 10 17609.0 11 19794.0 12 21945.0 13 24333.0 14 27206.0 15 28203.0 16 28631.0 17 29833.0 18 28791.0 19 27408.0 20 27764.0 21 29447.0 22 33051.0 23 38792.0 24 46867.0 25 58178.0 26 71812.0 27 85948.0 28 106335.0 29 133204.0 30 164124.0 31 198924.0 32 254457.0 33 334500.0 34 460016.0 35 668030.0 36 1094395.0 37 1883121.0 38 2363266.0 39 789061.0 40 1894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.916539021717675 19.807379348850457 13.514927462462733 23.76115416696914 2 15.574827352843704 19.98934998439499 40.372589669806615 24.063232992954685 3 18.491504434991462 24.705404638406247 30.175155939537994 26.627934987064293 4 12.696314231047559 16.16974896468701 38.411117530963935 32.7228192733015 5 13.167443576603032 37.65129364621388 35.616919403727884 13.564343373455207 6 31.67652511318944 37.267738017450455 17.42551034461159 13.630226524748512 7 27.55505989202157 32.196070822539404 22.371293101571304 17.87757618386772 8 25.097618354545915 36.466609192159986 20.406703280744658 18.029069172549438 9 25.040505010008 16.698703861130827 21.33930274108391 36.92148838777726 10-11 24.08506043645356 26.698249543734164 28.95022819727159 20.26646182254069 12-13 24.31800891498905 24.23380679084956 28.9972412421693 22.45094305199209 14-15 21.74145810498039 26.469265684498964 25.958227009949685 25.83104920057096 16-17 21.563779692320693 29.81671879347867 26.654891546683302 21.964609967517333 18-19 21.498536671797368 28.904804995576285 28.466106847680976 21.130551484945368 20-21 22.42569226849516 28.195894613031676 28.066331988245196 21.312081130227973 22-23 22.167839750687826 27.93377366528622 28.11992013361403 21.778466450411923 24-25 22.00063267716985 28.066160936061905 28.039626890999823 21.893579495768414 26-27 21.737040193413332 28.081130724100927 28.1319680055462 22.049861076939546 28-29 21.809551327385982 28.09672629677083 28.260151907792242 21.83357046805095 30-31 21.997671551474962 28.422671859825467 27.872692532715988 21.706964055983583 32-33 21.701341798525046 28.225882306507337 28.20069415687482 21.872081738092795 34-35 21.896543116222848 27.971394546428492 28.227872137981265 21.90419019936739 36-37 22.11193463789772 28.196292225782603 27.82991203132327 21.861861104996404 38-39 21.982133059117317 28.31375275685336 27.924795657159624 21.779318526869694 40-41 21.873449720329617 28.075126107064918 28.13774460266052 21.913679569944946 42-43 21.779250427823797 28.1272696969476 28.07095761745152 22.022522257777084 44-45 21.86491802448822 28.183057452222 28.036843922016807 21.91518060127298 46-47 21.865794377488506 28.23996353545239 27.951759548483103 21.942482538576005 48-49 21.798193480799632 28.128609255201482 27.98359287349293 22.089604390505958 50-51 21.786758957365638 28.09946764894721 27.972928833834647 22.140844559852503 52-53 21.84803655440185 28.044616421810204 28.024919867790167 22.08242715599778 54-55 21.879633354073636 28.285694720307024 27.86559769473232 21.96907423088702 56-57 21.782047821240745 28.14259436993032 27.88440552914931 22.190952279679625 58-59 21.732097467852125 27.801369569180938 28.13681749444987 22.32971546851707 60-61 21.91073195966455 28.10818396456215 27.810864781358998 22.17021929441431 62-63 21.76284635043685 28.152499697961307 27.8707792405227 22.213874711079143 64-65 21.79058912707147 27.862670286986646 27.968285593741843 22.37845499220004 66-67 21.675090072285727 27.929403726267765 27.85348395241624 22.542022249030264 68-69 21.89731370937466 28.012381096552424 27.792523434547757 22.297781759525158 70-71 21.894524680998565 27.768879685494447 27.88645676749789 22.4501388660091 72-73 21.661151377110034 27.9170673187778 27.997024360434825 22.42475694367734 74-75 21.75705989381451 27.92019620469795 27.886306419320565 22.436437482166973 76-77 21.79425161895922 27.915235255492988 27.739564808104433 22.55094831744336 78-79 21.80216082899147 27.891575784871815 27.865187590781478 22.44107579535524 80-81 21.79194091612439 27.752220128276463 27.903326342233292 22.552512613365856 82-83 21.687472447874907 27.761737077936033 28.01453695833548 22.536253515853588 84-85 21.7370181317435 27.715388521656692 27.882431357786764 22.665161988813047 86-87 21.87642488528928 27.809562102034214 27.787603150098793 22.526409862577715 88-89 21.743333634700996 27.639591946040387 27.877549063674266 22.739525355584348 90-91 21.736546905932883 27.545969758644222 27.91298030409093 22.80450303133197 92-93 21.849111210779455 27.679258767829033 27.788436356008166 22.683193665383346 94-95 21.759001465871197 27.454449457473217 27.902372420514986 22.884176656140593 96-97 21.806772956846626 27.421545584339572 27.891046114982814 22.88063534383098 98-99 21.869068189612946 27.474475893980276 27.793070900348106 22.863385016058672 100 21.911121894203923 27.370405706481332 27.781551026394503 22.936921372920242 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1908.0 1 1562.0 2 1499.0 3 1891.5 4 1912.0 5 1612.0 6 1268.0 7 1098.5 8 1087.0 9 1125.5 10 1048.0 11 955.5 12 1020.0 13 1131.5 14 1240.0 15 1440.5 16 1792.5 17 2297.5 18 3086.0 19 4405.5 20 6484.5 21 9378.5 22 13749.5 23 20276.0 24 29264.5 25 41058.5 26 56819.5 27 76398.5 28 99105.0 29 126129.0 30 152534.0 31 176296.5 32 199081.0 33 220832.5 34 241724.5 35 260211.5 36 278064.0 37 296911.5 38 314818.5 39 324889.5 40 330049.0 41 336106.5 42 337856.0 43 336152.0 44 333466.5 45 330626.0 46 327352.0 47 318671.0 48 308205.0 49 301016.5 50 294050.5 51 284193.5 52 269050.5 53 255220.5 54 245661.0 55 234267.0 56 216656.0 57 195597.5 58 177255.5 59 160870.5 60 141541.5 61 120387.5 62 102610.0 63 86652.0 64 70690.5 65 56138.0 66 44336.0 67 34532.0 68 26613.0 69 20471.0 70 15084.5 71 11045.0 72 8330.0 73 5836.5 74 4026.5 75 2942.5 76 2013.0 77 1354.5 78 959.5 79 682.0 80 522.5 81 366.0 82 260.0 83 193.5 84 155.5 85 125.5 86 100.0 87 86.0 88 63.5 89 46.0 90 44.5 91 37.0 92 30.5 93 31.5 94 27.5 95 29.5 96 26.5 97 20.0 98 23.0 99 34.0 100 68.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014580761821218632 2 0.03676281785658727 3 0.03809224025793368 4 0.04127642133212628 5 0.04394598728321704 6 0.04343137216011521 7 0.04106199836416718 8 0.040793969654218304 9 0.04096550802858558 10-11 0.04124425788693241 12-13 0.042455747655901314 14-15 0.040724282189631596 16-17 0.03712197632791876 18-19 0.04020430649233079 20-21 0.039480628975468834 22-23 0.04060098898305512 24-25 0.03740072618626559 26-27 0.037448971354056386 28-29 0.03800647107075004 30-31 0.03810296140633163 32-33 0.03784565384478072 34-35 0.03797966819975515 36-37 0.03539587143584803 38-39 0.035626376126404055 40-41 0.03684322646957193 42-43 0.036741375559791366 44-45 0.03564245784900099 46-47 0.03467219391898607 48-49 0.034736520809373804 50-51 0.03583543852016417 52-53 0.0363768565142609 54-55 0.03562101555220508 56-57 0.03405036731190469 58-59 0.03494022262893494 60-61 0.0343559200412464 62-63 0.03454890071240959 64-65 0.03165955121916075 66-67 0.03261909400077771 68-69 0.03371265113736911 70-71 0.03498310722252677 72-73 0.03191685878071167 74-75 0.03232962299403293 76-77 0.033037218788297955 78-79 0.033380295537032506 80-81 0.03348214644681308 82-83 0.03202943083889019 84-85 0.033278444627251935 86-87 0.033701929988971153 88-89 0.03430767487345561 90-91 0.034736520809373804 92-93 0.03324628118205807 94-95 0.03442560750583311 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9327359.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.35557053771968 #Duplication Level Percentage of deduplicated Percentage of total 1 72.91594794594822 40.36303899846632 2 15.167031558909084 16.791593706140244 3 5.207167870146772 8.647372450129714 4 2.3081064402317866 5.110661954432628 5 1.2106323477508973 3.350762216058498 6 0.7156636604729665 2.3769582143156374 7 0.48283906462583254 1.8709482330183305 8 0.3515349168619507 1.5567532709458507 9 0.24167612007281164 1.204030756077564 >10 1.2890057761379847 12.898647075459927 >50 0.07953155394110308 3.0101729048028507 >100 0.030590866298355442 2.6896509541440285 >500 1.9430171788368974E-4 0.0629548194846777 >1k 7.757688447181381E-5 0.06645444652370491 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0721148397954877E-5 0.0 0.0 0.0 3 0.0 1.0721148397954877E-5 0.0 0.0 0.0 4 0.0 1.0721148397954877E-5 0.0 0.0 0.0 5 0.0 2.1442296795909754E-5 0.0 3.216344519386463E-5 0.0 6 0.0 2.1442296795909754E-5 0.0 3.216344519386463E-5 0.0 7 0.0 2.1442296795909754E-5 0.0 3.216344519386463E-5 0.0 8 0.0 2.1442296795909754E-5 0.0 3.216344519386463E-5 0.0 9 0.0 2.1442296795909754E-5 0.0 3.216344519386463E-5 0.0 10-11 0.0 2.6802870994887195E-5 0.0 4.288459359181951E-5 1.0721148397954877E-5 12-13 0.0 4.288459359181951E-5 0.0 6.432689038772927E-5 1.0721148397954877E-5 14-15 0.0 1.1793263237750364E-4 0.0 7.504803878568414E-5 1.0721148397954877E-5 16-17 0.0 1.71538374367278E-4 0.0 9.649033558159389E-5 1.0721148397954877E-5 18-19 0.0 1.983412453621652E-4 0.0 1.125720581785262E-4 1.0721148397954877E-5 20-21 0.0 2.0370181956114264E-4 0.0 1.5009607757136828E-4 1.0721148397954877E-5 22-23 0.0 2.0370181956114264E-4 0.0 3.645190455304658E-4 1.0721148397954877E-5 24-25 0.0 2.3586526475500728E-4 0.0 6.432689038772927E-4 1.0721148397954877E-5 26-27 0.0 2.5730756155091706E-4 0.0 0.0016832202984789157 1.0721148397954877E-5 28-29 0.0 2.680287099488719E-4 0.0 0.006266511238604625 1.0721148397954877E-5 30-31 0.0 2.680287099488719E-4 0.0 0.01964650443925231 1.0721148397954877E-5 32-33 0.0 2.680287099488719E-4 0.0 0.04885627324948037 1.0721148397954877E-5 34-35 0.0 2.733892841478493E-4 0.0 0.09592211471650228 1.0721148397954877E-5 36-37 0.0 3.05552729341714E-4 0.0 0.1703376057467071 1.0721148397954877E-5 38-39 0.0 3.484373229335335E-4 0.0 0.26895072871109604 1.0721148397954877E-5 40-41 0.0 3.6987961972944326E-4 0.0 0.3699171437488361 1.0721148397954877E-5 42-43 1.0721148397954877E-5 3.9668249072433046E-4 0.0 0.47149466424525954 1.0721148397954877E-5 44-45 1.0721148397954877E-5 4.0204306492330787E-4 0.0 0.5949808514929038 1.0721148397954877E-5 46-47 1.0721148397954877E-5 4.074036391222853E-4 0.0 0.7387568120836777 1.0721148397954877E-5 48-49 1.0721148397954877E-5 4.074036391222853E-4 0.0 0.886923082943414 1.0721148397954877E-5 50-51 1.0721148397954877E-5 4.181247875202402E-4 0.0 1.0344139214540793 1.0721148397954877E-5 52-53 2.1442296795909754E-5 4.181247875202402E-4 0.0 1.1769515894048894 1.0721148397954877E-5 54-55 2.1442296795909754E-5 4.2884593591819507E-4 0.0 1.3356460279914175 1.0721148397954877E-5 56-57 2.1442296795909754E-5 4.3956708431614996E-4 0.0 1.520414299481772 1.0721148397954877E-5 58-59 2.1442296795909754E-5 4.502882327141048E-4 0.0 1.7182194874240393 1.0721148397954877E-5 60-61 2.1442296795909754E-5 4.502882327141048E-4 0.0 1.9189354671563517 1.0721148397954877E-5 62-63 2.1442296795909754E-5 4.502882327141048E-4 0.0 2.1156310162394307 1.0721148397954877E-5 64-65 2.1442296795909754E-5 4.5564880691308226E-4 0.0 2.3221042526614446 1.0721148397954877E-5 66-67 2.1442296795909754E-5 4.610093811120597E-4 0.0 2.5578247819130797 1.0721148397954877E-5 68-69 2.1442296795909754E-5 4.8245167790796946E-4 0.0 2.8119910469833957 1.0721148397954877E-5 70-71 2.1442296795909754E-5 4.931728263059243E-4 0.0 3.075356057379157 1.0721148397954877E-5 72-73 2.1442296795909754E-5 4.931728263059243E-4 0.0 3.338222534374414 2.1442296795909754E-5 74-75 2.1442296795909754E-5 4.985334005049018E-4 0.0 3.6046805960829857 2.1442296795909754E-5 76-77 2.1442296795909754E-5 5.092545489028567E-4 0.0 3.890157975049529 2.1442296795909754E-5 78-79 2.1442296795909754E-5 5.25336271499789E-4 0.0 4.205799305033718 2.1442296795909754E-5 80-81 2.1442296795909754E-5 5.360574198977438E-4 0.0 4.536487766794437 2.1442296795909754E-5 82-83 2.1442296795909754E-5 5.467785682956987E-4 0.0 4.872220528876395 2.1442296795909754E-5 84-85 2.1442296795909754E-5 5.574997166936536E-4 0.0 5.2033968028892215 2.1442296795909754E-5 86-87 2.1442296795909754E-5 5.789420134895633E-4 0.0 5.548917973458511 2.1442296795909754E-5 88 2.1442296795909754E-5 6.003843102854731E-4 0.0 5.825207328248006 2.1442296795909754E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8145 0.0 41.845814 1 GTATCAA 13215 0.0 34.400475 1 TCAACGC 14050 0.0 31.083208 4 ATCAACG 14260 0.0 30.658428 3 CAACGCA 14815 0.0 29.573364 5 AACGCAG 15755 0.0 27.984035 6 TATCAAC 16580 0.0 26.652 2 GTACATG 25040 0.0 26.490967 1 TACATGG 25165 0.0 24.919846 2 ACATGGG 25840 0.0 23.432026 3 ACGCAGA 18725 0.0 23.243727 7 CGCAGAG 19390 0.0 21.961752 8 CATGGGG 18940 0.0 20.377441 4 GAGTACA 18040 0.0 20.013811 1 GCAGAGT 22510 0.0 18.186924 9 AGTACAT 19380 0.0 17.27081 2 AGAGTAC 19000 0.0 16.66098 10-11 CAGAGTA 21180 0.0 16.044601 10-11 GTGGTAT 5715 0.0 15.629406 1 GTATAGA 4165 0.0 15.576475 1 >>END_MODULE