FastQCFastQC Report
Wed 25 May 2016
SRR2049381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9327359
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174430.1870089915055269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163500.17529077630656223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93940.10071446805038811No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129700.054.0071531
GTATCAA232500.039.6515051
ATCAACG283900.031.7336253
TCAACGC284250.031.7110864
CAACGCA293750.030.7493675
TATCAAC301500.030.2708682
AACGCAG306500.029.5119866
GTACATG241700.025.7523211
ACGCAGA357350.025.0887347
CGCAGAG363500.024.5736798
GTGGTAT64700.024.1234171
TACATGG251650.023.8856242
ACATGGG255650.022.555983
GCAGAGT399250.022.0313769
TGGTATC67250.020.755212
AGAGTAC364850.020.13778110-11
CAGAGTA387750.019.56108310-11
GAGTACT274250.019.52674512-13
CATGGGG181200.019.2964654
AGTACTT286900.018.2478812-13
Produced by FastQC (version 0.11.5)