##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049381_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9327359
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873715271386036	33.0	31.0	34.0	30.0	34.0
2	31.982124843699058	33.0	31.0	34.0	30.0	34.0
3	31.967082214804854	33.0	31.0	34.0	30.0	34.0
4	35.6854107363081	37.0	35.0	37.0	33.0	37.0
5	35.77939028614638	37.0	35.0	37.0	35.0	37.0
6	35.83268350666035	37.0	35.0	37.0	35.0	37.0
7	35.77434620024811	37.0	35.0	37.0	33.0	37.0
8	35.76468033448697	37.0	35.0	37.0	33.0	37.0
9	37.48840470276742	39.0	37.0	39.0	34.0	39.0
10-11	37.461841503044965	39.0	37.0	39.0	34.5	39.0
12-13	37.416958112151576	39.0	37.0	39.0	34.0	39.0
14-15	38.71577720981898	40.0	38.0	41.0	34.0	41.0
16-17	38.739430100203066	40.0	38.0	41.0	35.0	41.0
18-19	38.79806234540774	40.0	38.0	41.0	35.0	41.0
20-21	38.82378318450057	40.0	38.0	41.0	35.0	41.0
22-23	38.79365477408986	40.0	38.0	41.0	35.0	41.0
24-25	38.778738976381206	40.0	38.0	41.0	35.0	41.0
26-27	38.66613175283594	40.0	38.0	41.0	34.0	41.0
28-29	38.58852822111811	40.0	38.0	41.0	34.0	41.0
30-31	38.50061035497829	40.0	38.0	41.0	34.0	41.0
32-33	38.380410950195014	40.0	38.0	41.0	34.0	41.0
34-35	38.29069638040093	40.0	38.0	41.0	34.0	41.0
36-37	38.18811439551109	40.0	38.0	41.0	34.0	41.0
38-39	38.106778027949815	40.0	38.0	41.0	33.5	41.0
40-41	38.00564999159998	40.0	38.0	41.0	33.0	41.0
42-43	37.884068255548	40.0	37.5	41.0	33.0	41.0
44-45	37.7244039818774	40.0	37.0	41.0	33.0	41.0
46-47	37.52419098482218	40.0	37.0	41.0	33.0	41.0
48-49	37.34135064384249	40.0	37.0	41.0	32.0	41.0
50-51	37.20108087401803	40.0	37.0	41.0	32.0	41.0
52-53	37.061219794370516	40.0	36.0	41.0	31.5	41.0
54-55	36.920119725208394	40.0	36.0	41.0	31.0	41.0
56-57	36.77594617082928	39.0	36.0	41.0	31.0	41.0
58-59	36.580613708553514	39.0	35.0	41.0	31.0	41.0
60-61	36.3981606690597	39.0	35.0	41.0	31.0	41.0
62-63	36.19109385625663	39.0	35.0	41.0	30.5	41.0
64-65	35.96107140295554	38.0	35.0	40.0	30.0	41.0
66-67	35.68969147644044	37.5	35.0	40.0	30.0	41.0
68-69	35.39349337792187	37.0	35.0	40.0	30.0	41.0
70-71	35.06034837942873	36.5	34.0	39.5	29.0	41.0
72-73	34.759018227989294	36.0	34.0	39.0	29.0	41.0
74-75	34.448462742776385	36.0	34.0	39.0	29.0	40.5
76-77	34.11957473707187	35.0	34.0	38.5	29.0	40.0
78-79	33.7829738299984	35.0	34.0	37.0	29.0	39.0
80-81	33.46557717999275	35.0	34.0	37.0	29.0	39.0
82-83	33.167577124457196	35.0	34.0	36.0	28.5	39.0
84-85	32.900696327867294	35.0	33.0	36.0	28.0	37.0
86-87	32.671267611764485	35.0	33.0	36.0	27.5	37.0
88-89	32.44867035781511	35.0	33.0	35.0	27.0	36.5
90-91	32.25911021544255	35.0	33.0	35.0	27.0	36.0
92-93	32.07914303502203	35.0	33.0	35.0	27.0	36.0
94-95	31.922743833490273	35.0	33.0	35.0	26.5	36.0
96-97	31.721447625206665	35.0	33.0	35.0	26.0	36.0
98-99	31.570008562981226	35.0	33.0	35.0	25.5	35.0
100	31.45475133958069	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1787394478076969
1101	2	0.20184841438575418
1101	3	0.38300524074892195
1101	4	0.19941044993653634
1101	5	0.16052715871240508
1101	6	0.1760101105098002
1101	7	0.0543684641593245
1101	8	0.12951042626832532
1101	9	0.2552259669118797
1101	10-11	0.2679257154674346
1101	12-13	0.19175269643292836
1101	14-15	0.3088521755007818
1101	16-17	0.21322973548679158
1101	18-19	0.3027095845629191
1101	20-21	0.2519581548443952
1101	22-23	0.2741396034563337
1101	24-25	0.23721850663366695
1101	26-27	0.3261308038206252
1101	28-29	0.32714754175321303
1101	30-31	0.3192886214437607
1101	32-33	0.34509506287798786
1101	34-35	0.39509846950393523
1101	36-37	0.4065667038212979
1101	38-39	0.38278766486581617
1101	40-41	0.397671839047959
1101	42-43	0.40199103197680586
1101	44-45	0.43590492709666506
1101	46-47	0.46204855076085494
1101	48-49	0.45839719655926814
1101	50-51	0.3871354409492369
1101	52-53	0.39858281805667417
1101	54-55	0.37423230886767556
1101	56-57	0.3782002853219879
1101	58-59	0.39639768703949585
1101	60-61	0.43700222359788654
1101	62-63	0.42676900694586095
1101	64-65	0.36012834439407015
1101	66-67	0.35686715903388944
1101	68-69	0.35643775101473807
1101	70-71	0.382187079284094
1101	72-73	0.3389368670595161
1101	74-75	0.3917317531762521
1101	76-77	0.3297120663740145
1101	78-79	0.3965839160246105
1101	80-81	0.4735720341354792
1101	82-83	0.4278177869164992
1101	84-85	0.43216842940375244
1101	86-87	0.3392212981153975
1101	88-89	0.4082182502108367
1101	90-91	0.4308197256358781
1101	92-93	0.4831453933801271
1101	94-95	0.49536072084871563
1101	96-97	0.4975688752151832
1101	98-99	0.5430768803033317
1101	100	0.5325621036356374
1102	1	0.21268026272770513
1102	2	0.14693370938021033
1102	3	0.3414936516230469
1102	4	0.18184225767005557
1102	5	0.156563838305658
1102	6	0.17668304566664972
1102	7	0.17159164780573377
1102	8	0.16177681178062642
1102	9	0.09978461153218632
1102	10-11	0.17504574312786048
1102	12-13	0.20107630129135856
1102	14-15	0.26142111465266993
1102	16-17	0.20821807756651367
1102	18-19	0.18012788167013838
1102	20-21	0.17292386057521725
1102	22-23	0.23852898329813854
1102	24-25	0.2773130308567815
1102	26-27	0.29967960923508485
1102	28-29	0.28164370837440345
1102	30-31	0.23170984019364482
1102	32-33	0.2445975338342663
1102	34-35	0.2142082006883257
1102	36-37	0.24732908755238014
1102	38-39	0.2216351988323595
1102	40-41	0.19412511438825675
1102	42-43	0.23921190645514656
1102	44-45	0.24995608721907558
1102	46-47	0.19695885107781663
1102	48-49	0.17170914034375784
1102	50-51	0.25918558461719954
1102	52-53	0.300438237824288
1102	54-55	0.23401840754584669
1102	56-57	0.25323394803047705
1102	58-59	0.23023903491843356
1102	60-61	0.24700631784123317
1102	62-63	0.21129478083025788
1102	64-65	0.23423477604208642
1102	66-67	0.16812652085801716
1102	68-69	0.2068549814457512
1102	70-71	0.24166813164616485
1102	72-73	0.27848074201551043
1102	74-75	0.22682910388501654
1102	76-77	0.21437668626536777
1102	78-79	0.26754194144127297
1102	80-81	0.21435209483313855
1102	82-83	0.23981288084885222
1102	84-85	0.24652500346676476
1102	86-87	0.2780043422348868
1102	88-89	0.26919118058371083
1102	90-91	0.26773510717990234
1102	92-93	0.3471048020189613
1102	94-95	0.3696379095269364
1102	96-97	0.37912220072194813
1102	98-99	0.39297453576492103
1102	100	0.4184666632922287
1103	1	0.14471814860970866
1103	2	0.15215485231083292
1103	3	0.3052375026852161
1103	4	0.18650779420848806
1103	5	0.15381274560426306
1103	6	0.13545754776688312
1103	7	0.11491074886372132
1103	8	0.11572771794579495
1103	9	0.17717916359385555
1103	10-11	0.1523677325259598
1103	12-13	0.197939867186669
1103	14-15	0.2562334787938312
1103	16-17	0.21836491265557356
1103	18-19	0.23399570400300718
1103	20-21	0.24507526278440395
1103	22-23	0.21958307368033303
1103	24-25	0.2516694270104054
1103	26-27	0.2509226821511632
1103	28-29	0.267981055166004
1103	30-31	0.23101982543924748
1103	32-33	0.2555750546403601
1103	34-35	0.29450519306799094
1103	36-37	0.29620655025880893
1103	38-39	0.29434546110660875
1103	40-41	0.30777948944667344
1103	42-43	0.3254070706219494
1103	44-45	0.356318728005931
1103	46-47	0.3507499471975848
1103	48-49	0.2679999475201029
1103	50-51	0.306424774087553
1103	52-53	0.3094103616796957
1103	54-55	0.2713832440723962
1103	56-57	0.25341606445081055
1103	58-59	0.2773191255444587
1103	60-61	0.27119403265202635
1103	62-63	0.2596245611336272
1103	64-65	0.2698374060693709
1103	66-67	0.2588690873240793
1103	68-69	0.28540442037146363
1103	70-71	0.32858929964446304
1103	72-73	0.32332255621512473
1103	74-75	0.27368722779650767
1103	76-77	0.26284191437864024
1103	78-79	0.2742139950000677
1103	80-81	0.2620406366815047
1103	82-83	0.2500400902957196
1103	84-85	0.2346653342046281
1103	86-87	0.2087010641623479
1103	88-89	0.2468007140410009
1103	90-91	0.27977892280871686
1103	92-93	0.333831901388038
1103	94-95	0.30084720586345526
1103	96-97	0.3648297832942653
1103	98-99	0.38451224805051964
1103	100	0.44358777622571566
1104	1	0.13887408891057262
1104	2	0.1813129642072333
1104	3	0.2756934938759983
1104	4	0.1664911672447289
1104	5	0.15481189890135738
1104	6	0.15832883576432266
1104	7	0.18187398089980178
1104	8	0.15133448147878426
1104	9	0.1473537796162816
1104	10-11	0.1598516758802191
1104	12-13	0.15940781092699297
1104	14-15	0.2065727357571916
1104	16-17	0.22813972477965905
1104	18-19	0.21565767651368617
1104	20-21	0.23401076420362443
1104	22-23	0.2472945245693694
1104	24-25	0.23116136688133082
1104	26-27	0.26229210893768595
1104	28-29	0.2905284409159492
1104	30-31	0.2872017711449786
1104	32-33	0.3138030710742896
1104	34-35	0.2840652749744379
1104	36-37	0.3152592849337026
1104	38-39	0.32681747713693454
1104	40-41	0.3205125246791596
1104	42-43	0.379311144228069
1104	44-45	0.36287105139380316
1104	46-47	0.4362317068919026
1104	48-49	0.3863228396466454
1104	50-51	0.361826343778219
1104	52-53	0.31112106896177494
1104	54-55	0.32118483920801566
1104	56-57	0.32689674273441227
1104	58-59	0.4146745718621716
1104	60-61	0.3745111722439418
1104	62-63	0.4122027550242038
1104	64-65	0.30551375936821756
1104	66-67	0.35823186661539097
1104	68-69	0.3338155911466103
1104	70-71	0.3613252248538288
1104	72-73	0.36599174873254725
1104	74-75	0.37562491068214143
1104	76-77	0.3279190389229427
1104	78-79	0.3462301624191113
1104	80-81	0.3758189167778525
1104	82-83	0.3691119086889145
1104	84-85	0.37252948774301586
1104	86-87	0.3554637416738373
1104	88-89	0.3173651204934913
1104	90-91	0.26949776150962634
1104	92-93	0.3762084220532884
1104	94-95	0.43059691302709524
1104	96-97	0.48296734948405273
1104	98-99	0.44823228990308905
1104	100	0.430436023375222
1105	1	0.18790568449913891
1105	2	0.16372907742790588
1105	3	0.2942628885229084
1105	4	0.17962796631820055
1105	5	0.13780644092374672
1105	6	0.11372125712624381
1105	7	0.12722368984687904
1105	8	0.11688587253186711
1105	9	0.15642266662253235
1105	10-11	0.15044782167731086
1105	12-13	0.1499697077885287
1105	14-15	0.21263241253129195
1105	16-17	0.21864692840239286
1105	18-19	0.16792634281391372
1105	20-21	0.17085518074464545
1105	22-23	0.15663852718153493
1105	24-25	0.1943211631695121
1105	26-27	0.22369087959000922
1105	28-29	0.20928760198091823
1105	30-31	0.16976688824691877
1105	32-33	0.2179332300565875
1105	34-35	0.27236040708707776
1105	36-37	0.24200293396238237
1105	38-39	0.2621088305203898
1105	40-41	0.26174699289194336
1105	42-43	0.20701034906443283
1105	44-45	0.2755324941522659
1105	46-47	0.24991387298788936
1105	48-49	0.27309543848108575
1105	50-51	0.2732187163931883
1105	52-53	0.2788069146121259
1105	54-55	0.24571094320039322
1105	56-57	0.20856663888660165
1105	58-59	0.22645643681967442
1105	60-61	0.22068439999810607
1105	62-63	0.2049883848432188
1105	64-65	0.17031799833586092
1105	66-67	0.19920430945959566
1105	68-69	0.12695868109818775
1105	70-71	0.15206153914728304
1105	72-73	0.15800282731768078
1105	74-75	0.14625824493998607
1105	76-77	0.15708892036699496
1105	78-79	0.1770541308747937
1105	80-81	0.20588067832456858
1105	82-83	0.22911614883320652
1105	84-85	0.2079485497340059
1105	86-87	0.18110304112971676
1105	88-89	0.20354878471776772
1105	90-91	0.2431364713795361
1105	92-93	0.2680702971467923
1105	94-95	0.25922500992930964
1105	96-97	0.29189553219771014
1105	98-99	0.2772501943220824
1105	100	0.29615321709728804
1106	1	0.14189216587093156
1106	2	0.09750198382384312
1106	3	0.2385664469059776
1106	4	0.12866997096177357
1106	5	0.1081993873135616
1106	6	0.10949693521680359
1106	7	0.12648643283996108
1106	8	0.09168246948961212
1106	9	0.13840821926406477
1106	10-11	0.13686729983172796
1106	12-13	0.13369859437378295
1106	14-15	0.21208378858760568
1106	16-17	0.19636319463150897
1106	18-19	0.21157063322193181
1106	20-21	0.19158073211816884
1106	22-23	0.15985021122545362
1106	24-25	0.17054854649278184
1106	26-27	0.2107331776989838
1106	28-29	0.20600813524669093
1106	30-31	0.21294206970622298
1106	32-33	0.15022579446088002
1106	34-35	0.20333452003424668
1106	36-37	0.18759959082664324
1106	38-39	0.22673858382418643
1106	40-41	0.18928740892548035
1106	42-43	0.17802789688673215
1106	44-45	0.17548595075084705
1106	46-47	0.19244539018285423
1106	48-49	0.18850302271516028
1106	50-51	0.19220848627143994
1106	52-53	0.1883386094662498
1106	54-55	0.1375407789937384
1106	56-57	0.12935153790693477
1106	58-59	0.20462727004764503
1106	60-61	0.1383496868439451
1106	62-63	0.16090266965353095
1106	64-65	0.127509755520947
1106	66-67	0.13659779489407953
1106	68-69	0.14935432731270737
1106	70-71	0.21215314640725325
1106	72-73	0.2567536509203805
1106	74-75	0.21575731289109257
1106	76-77	0.17623231574014397
1106	78-79	0.22795162724067808
1106	80-81	0.1892591125712002
1106	82-83	0.23306240239733
1106	84-85	0.2010471602178754
1106	86-87	0.1907738347986978
1106	88-89	0.21591714316953414
1106	90-91	0.17523249295858534
1106	92-93	0.22540701573610988
1106	94-95	0.19879270264989657
1106	96-97	0.2719404751193615
1106	98-99	0.28497140980228153
1106	100	0.31605336393586114
1107	1	0.10451385952194414
1107	2	0.09487240840071465
1107	3	0.2047529980547722
1107	4	0.16298490490336803
1107	5	0.11853200266365604
1107	6	0.09677738369195055
1107	7	0.09485234824723676
1107	8	0.08405564355290096
1107	9	0.11958149538886431
1107	10-11	0.12833575896841154
1107	12-13	0.11658971752868297
1107	14-15	0.15663773256818558
1107	16-17	0.11495814197608922
1107	18-19	0.10634364497611415
1107	20-21	0.09294866351893205
1107	22-23	0.04722559504963897
1107	24-25	0.08904618013735188
1107	26-27	0.18395572584227438
1107	28-29	0.19992453140558553
1107	30-31	0.14516799300238858
1107	32-33	0.2034111789425168
1107	34-35	0.1617245282555686
1107	36-37	0.14227691754709326
1107	38-39	0.1420170215548211
1107	40-41	0.16903323704727313
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2216	72-73	-0.7007622117188177
2216	74-75	-0.6531809994377866
2216	76-77	-0.6757181491819892
2216	78-79	-0.8378797694116216
2216	80-81	-1.0172571432680115
2216	82-83	-0.8742735514137081
2216	84-85	-0.9585881222027872
2216	86-87	-0.8019625673654893
2216	88-89	-1.0995662627720861
2216	90-91	-0.8832243683516658
2216	92-93	-0.9998763426023416
2216	94-95	-0.9683687080194403
2216	96-97	-0.9965074062221824
2216	98-99	-1.1118030340714888
2216	100	-1.0397988238064428
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	529.0
3	49.0
4	77.0
5	148.0
6	401.0
7	1139.0
8	2594.0
9	4337.0
10	6538.0
11	9217.0
12	13015.0
13	18045.0
14	21454.0
15	26983.0
16	33675.0
17	45182.0
18	45035.0
19	26331.0
20	21108.0
21	21824.0
22	23604.0
23	26520.0
24	31862.0
25	39742.0
26	50550.0
27	65728.0
28	86993.0
29	114350.0
30	149749.0
31	193985.0
32	249365.0
33	326341.0
34	445050.0
35	640770.0
36	1045438.0
37	1900831.0
38	2626008.0
39	1009284.0
40	3508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24170004780199	20.04679597407903	13.922081142408649	23.78942283571033
2	15.69224471793141	20.317798425041858	39.705622995748314	24.28433386127842
3	18.1147632464881	24.881448221302517	30.60999367559456	26.39379485661483
4	12.708135081367109	16.69370183790562	37.73748959512213	32.86067348560514
5	12.822043195721319	37.73016563423794	35.55335438466559	13.894436785375152
6	31.512650043811973	37.67364373988393	16.943617158940704	13.870089057363396
7	27.162297494928627	32.591540649394965	22.127153034422715	18.11900882125369
8	25.124310074929664	36.43934389882754	20.046924714715896	18.389421311526906
9	24.951789675941498	16.275786104083696	21.322841760459742	37.44958245951507
10-11	24.07252685352842	26.73755239827265	28.618379543448473	20.571541204750453
12-13	24.527069419713044	24.19073188581181	29.02234940522449	22.259849289250656
14-15	21.751572338169755	26.222343299828644	26.455303333175735	25.570781028825866
16-17	21.12467318587572	29.633223597013025	27.152087056587348	22.090016160523902
18-19	21.026616980549896	28.168637107586996	29.760910757756164	21.04383515410694
20-21	21.940423176910233	27.431531478601855	29.577253008142073	21.050792336345836
22-23	21.66077235796328	27.204597785932762	29.652788104328355	21.48184175177561
24-25	21.554605125087743	27.329400277302696	29.590218660654244	21.525775936955316
26-27	21.307326324738813	27.347884971708226	29.679199065758976	21.665589637793982
28-29	21.35248857872755	27.352082031603466	29.792125979862195	21.503303409806794
30-31	21.586335424636278	27.624513005235457	29.450174481329604	21.33897708879866
32-33	21.354809043174562	27.457071380663134	29.704474285498637	21.483645290663667
34-35	21.49654660903832	27.235810927729343	29.7049838571582	21.562658606074134
36-37	21.721172737546027	27.468991612896065	29.26949122932004	21.54034442023787
38-39	21.62287904224692	27.618173944936657	29.32464817641831	21.434298836398106
40-41	21.514128212763598	27.373044694646577	29.4712832345959	21.641543857993923
42-43	21.478043667257083	27.400818392528866	29.343806450499283	21.777331489714772
44-45	21.584933620389187	27.569675031576534	29.175540671469253	21.669850676565027
46-47	21.686562274051628	27.67029163260913	28.86392086414236	21.779225229196882
48-49	21.66700358137385	27.650876811814456	28.695167667466848	21.986951939344845
50-51	21.677636496939716	27.66014520026616	28.612111651095663	22.05010665169846
52-53	21.685509011336286	27.76791843562134	28.552523252424304	21.99404930061807
54-55	21.692157892609533	27.975839165926104	28.423319992962615	21.908682948501752
56-57	21.62439826623268	27.782373894490686	28.447527550388678	22.14570028888796
58-59	21.53681641850499	27.470316204912788	28.714474046684302	22.27839332989792
60-61	21.689848761430028	27.76987181621103	28.420185176935846	22.120094245423097
62-63	21.531575778394462	27.81084209366164	28.47465326425421	22.18292886368968
64-65	21.498800165427877	27.57749741284414	28.596696106146158	22.32700631558182
66-67	21.48137613476933	27.639991783194702	28.429631704002883	22.449000378033084
68-69	21.730389768480233	27.738709613956104	28.263576813082448	22.26732380448122
70-71	21.740482703473095	27.577823663291078	28.286590988269356	22.39510264496647
72-73	21.539076525994723	27.747378559971764	28.317647336945427	22.395897577088082
74-75	21.670175819294712	27.75725344448119	28.15492584088462	22.417644895339482
76-77	21.770256699234515	27.757752326317046	27.999606960087153	22.47238401436129
78-79	21.748665347099816	27.80860358802603	28.04317935235796	22.399551712516192
80-81	21.69629739186755	27.65640970741809	28.11755446982022	22.52973843089414
82-83	21.62295598698007	27.680364180615058	28.18322998014418	22.51344985226069
84-85	21.685659987684268	27.6335465454971	27.99134821674638	22.68944525007225
86-87	21.75153846512597	27.760393501417813	27.926434142939154	22.561633890517065
88-89	21.619388246807596	27.576405356969495	28.013675950058413	22.790530446164496
90-91	21.52645937502228	27.498824527515275	28.05064124697702	22.92407485048542
92-93	21.615533016279613	27.677148352021835	27.906811808910913	22.800506822787643
94-95	21.480126396381692	27.44182418637489	28.0464822184393	23.03156719880412
96-97	21.536493405676836	27.452308068054126	28.021527219330434	22.9896713069386
98-99	21.532934873892597	27.48048185237645	27.946109445294237	23.040473828436717
100	21.575859070699448	27.417829301947094	27.902635616361987	23.10367601099147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	877.0
1	651.5
2	770.5
3	1163.5
4	793.0
5	549.5
6	1314.5
7	3154.5
8	5212.0
9	5995.5
10	4936.0
11	3583.0
12	3442.5
13	4016.0
14	4738.5
15	5445.0
16	6216.5
17	7081.5
18	8238.0
19	9665.0
20	11675.0
21	14601.0
22	18848.0
23	25185.5
24	33608.0
25	44806.0
26	60009.0
27	78362.5
28	99958.0
29	125751.0
30	151736.0
31	175485.0
32	197200.0
33	218663.5
34	238653.0
35	256503.0
36	274707.0
37	293218.0
38	311243.5
39	321128.0
40	326189.0
41	333128.5
42	335734.0
43	334946.0
44	332794.5
45	330731.5
46	328103.5
47	318906.5
48	308110.0
49	301182.0
50	294030.5
51	284179.0
52	268860.0
53	255009.5
54	244665.0
55	231740.5
56	214384.0
57	193811.0
58	175447.0
59	159308.0
60	140191.5
61	118768.5
62	100895.5
63	84179.5
64	67815.0
65	53942.5
66	42478.5
67	32576.5
68	24531.5
69	18465.5
70	13409.5
71	9607.5
72	6920.5
73	4718.5
74	3235.5
75	2156.5
76	1338.0
77	847.5
78	530.5
79	303.5
80	182.0
81	101.0
82	54.5
83	33.5
84	23.0
85	14.5
86	9.0
87	6.0
88	5.0
89	4.0
90	2.0
91	2.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03730959642488297
2	0.0
3	0.0
4	3.2163445193864626E-5
5	0.0
6	0.0
7	0.0
8	8.362495750404804E-4
9	0.0
10-11	0.0
12-13	0.044337309199742395
14-15	8.5769187183639E-5
16-17	5.360574198977438E-6
18-19	5.3605741989774383E-5
20-21	7.504803878568413E-5
22-23	0.0
24-25	6.96874645867067E-5
26-27	1.6081722596932313E-5
28-29	2.894710067447817E-4
30-31	0.0
32-33	1.6081722596932314E-4
34-35	5.3605741989774383E-5
36-37	3.7524019392842064E-5
38-39	3.001921551427365E-4
40-41	1.232932065764811E-4
42-43	5.360574198977439E-4
44-45	2.3586526475500728E-4
46-47	7.504803878568413E-5
48-49	7.772832588517286E-4
50-51	3.6987961972944326E-4
52-53	5.574997166936536E-4
54-55	3.43076748734556E-4
56-57	4.342065101171725E-4
58-59	5.896631618875182E-5
60-61	1.0185090978057134E-4
62-63	8.898553170302549E-4
64-65	3.377161745355786E-4
66-67	0.0014259127369279985
68-69	0.00152776364670857
70-71	4.1276421332126276E-4
72-73	9.702639300149163E-4
74-75	8.952158912292322E-4
76-77	6.647112006732024E-4
78-79	0.0033074742807690793
80-81	0.0026213207832999673
82-83	0.0012758166593566302
84-85	0.002835743751259065
86-87	0.0013347829755453822
88-89	0.0019566095826267648
90-91	0.0027714168608713357
92-93	0.002498027576723486
94-95	0.0037684836618811393
96-97	0.0030394455708202075
98-99	0.0028947100674478164
100	0.001383028143336179
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9327359.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.119315166659575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.23528194767287	37.83969392764291
2	16.043069167810707	17.043936947309117
3	5.528456093443739	8.810034048380329
4	2.4614576678760427	5.230037825171936
5	1.3074175803972137	3.4724563253775544
6	0.7816022638030847	2.4910906191558397
7	0.5223609845338607	1.9423220437754514
8	0.3566865537188778	1.515755637016218
9	0.26395036245740444	1.261877624255601
>10	1.3731725436820066	13.106895485268497
>50	0.09025367998615091	3.279029864513138
>100	0.0353609210693242	3.04951965457278
>500	5.260816008597639E-4	0.18024178618175035
>1k	3.4352920029525626E-4	0.3135333630882528
>5k	2.020758251522426E-5	0.10083641199528988
>10k+	4.041516503044852E-5	0.36273843629517044
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17443	0.1870089915055269	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16350	0.17529077630656223	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9394	0.10071446805038811	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1442296795909754E-5	0.0	0.0	0.0
3	0.0	2.1442296795909754E-5	0.0	1.0721148397954877E-5	0.0
4	0.0	2.1442296795909754E-5	0.0	1.0721148397954877E-5	0.0
5	0.0	3.216344519386463E-5	0.0	1.0721148397954877E-5	0.0
6	0.0	3.216344519386463E-5	0.0	1.0721148397954877E-5	0.0
7	0.0	3.216344519386463E-5	0.0	1.0721148397954877E-5	0.0
8	0.0	3.216344519386463E-5	0.0	1.0721148397954877E-5	0.0
9	0.0	3.216344519386463E-5	0.0	1.0721148397954877E-5	0.0
10-11	0.0	3.216344519386463E-5	0.0	2.1442296795909754E-5	0.0
12-13	0.0	4.8245167790796946E-5	0.0	4.288459359181951E-5	0.0
14-15	0.0	5.3605741989774383E-5	0.0	6.432689038772927E-5	0.0
16-17	0.0	7.504803878568414E-5	0.0	7.504803878568414E-5	0.0
18-19	0.0	8.576918718363902E-5	0.0	8.040861298466159E-5	0.0
20-21	0.0	8.576918718363902E-5	0.0	1.232932065764811E-4	0.0
22-23	0.0	9.649033558159389E-5	0.0	3.645190455304658E-4	0.0
24-25	0.0	1.1793263237750364E-4	0.0	6.807929232701347E-4	0.0
26-27	5.3605741989774385E-6	1.393749291734134E-4	0.0	0.0017475471888666449	0.0
28-29	1.0721148397954877E-5	1.393749291734134E-4	0.0	0.006427328464573948	0.0
30-31	1.0721148397954877E-5	1.393749291734134E-4	0.0	0.019935975445997095	0.0
32-33	1.0721148397954877E-5	1.393749291734134E-4	0.0	0.0493708883725822	0.0
34-35	1.0721148397954877E-5	1.554566517703457E-4	0.0	0.09670475854955299	0.0
36-37	1.0721148397954877E-5	1.8762009696421034E-4	0.0	0.171549095515676	0.0
38-39	1.0721148397954877E-5	2.1442296795909753E-4	0.0	0.2709770257583095	0.0
40-41	1.0721148397954877E-5	2.5194698735193964E-4	0.0	0.3723401232867739	0.0
42-43	2.1442296795909754E-5	2.5730756155091706E-4	0.0	0.47467884531945215	0.0
44-45	2.1442296795909754E-5	2.626681357498945E-4	0.0	0.5995802241556265	0.0
46-47	2.1442296795909754E-5	2.733892841478493E-4	0.0	0.7443586121216091	0.0
48-49	2.1442296795909754E-5	2.894710067447817E-4	0.0	0.8934469017435697	0.0
50-51	2.1442296795909754E-5	3.5379789713251095E-4	0.0	1.0417364658098824	1.0721148397954877E-5
52-53	2.1442296795909754E-5	4.127642133212627E-4	0.0	1.185319445729493	1.0721148397954877E-5
54-55	2.1442296795909754E-5	4.181247875202402E-4	0.0	1.3452307346591892	1.0721148397954877E-5
56-57	2.1442296795909754E-5	4.2884593591819507E-4	0.0	1.531049678692543	1.0721148397954877E-5
58-59	2.1442296795909754E-5	4.502882327141048E-4	0.0	1.7302593370749428	1.0721148397954877E-5
60-61	2.1442296795909754E-5	4.502882327141048E-4	0.0	1.9323261815053971	1.0721148397954877E-5
62-63	2.1442296795909754E-5	4.502882327141048E-4	0.0	2.1304958884931953	1.0721148397954877E-5
64-65	2.1442296795909754E-5	4.5564880691308226E-4	0.0	2.3387327538266725	1.0721148397954877E-5
66-67	2.1442296795909754E-5	4.663699553110371E-4	0.0	2.576442056106128	1.0721148397954877E-5
68-69	2.1442296795909754E-5	4.7173052951001457E-4	0.0	2.8327632720044336	1.0721148397954877E-5
70-71	2.6802870994887195E-5	4.8245167790796946E-4	0.0	3.098797848351286	1.0721148397954877E-5
72-73	3.216344519386463E-5	5.092545489028567E-4	0.0	3.364521511394597	1.0721148397954877E-5
74-75	3.216344519386463E-5	5.199756973008115E-4	0.0	3.6336169756090655	1.0721148397954877E-5
76-77	3.216344519386463E-5	5.360574198977438E-4	0.0	3.9223696654111846	1.0721148397954877E-5
78-79	3.216344519386463E-5	5.950237360864957E-4	0.0	4.241591858960291	1.0721148397954877E-5
80-81	3.216344519386463E-5	6.057448844844506E-4	0.0	4.575866544860126	1.0721148397954877E-5
82-83	3.216344519386463E-5	6.164660328824053E-4	0.0	4.914992550410036	1.0721148397954877E-5
84-85	3.216344519386463E-5	6.218266070813828E-4	0.0	5.248940241283734	1.0721148397954877E-5
86-87	3.216344519386463E-5	6.271871812803603E-4	0.0	5.597602708333623	1.0721148397954877E-5
88	3.216344519386463E-5	6.432689038772926E-4	0.0	5.877237061423282	1.0721148397954877E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12970	0.0	54.007153	1
GTATCAA	23250	0.0	39.651505	1
ATCAACG	28390	0.0	31.733625	3
TCAACGC	28425	0.0	31.711086	4
CAACGCA	29375	0.0	30.749367	5
TATCAAC	30150	0.0	30.270868	2
AACGCAG	30650	0.0	29.511986	6
GTACATG	24170	0.0	25.752321	1
ACGCAGA	35735	0.0	25.088734	7
CGCAGAG	36350	0.0	24.573679	8
GTGGTAT	6470	0.0	24.123417	1
TACATGG	25165	0.0	23.885624	2
ACATGGG	25565	0.0	22.55598	3
GCAGAGT	39925	0.0	22.031376	9
TGGTATC	6725	0.0	20.75521	2
AGAGTAC	36485	0.0	20.137781	10-11
CAGAGTA	38775	0.0	19.561083	10-11
GAGTACT	27425	0.0	19.526745	12-13
CATGGGG	18120	0.0	19.296465	4
AGTACTT	28690	0.0	18.24788	12-13
>>END_MODULE