FastQCFastQC Report
Wed 25 May 2016
SRR2049380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6268854
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58150.042.525251
GTATCAA93200.033.7963451
TCAACGC94950.031.4875144
ATCAACG95800.031.1590673
CAACGCA101400.029.4848465
AACGCAG107950.027.9102086
TATCAAC113550.026.6195092
GTACATG161300.025.3277321
TACATGG162450.024.1046872
ACGCAGA128000.023.2076597
ACATGGG166600.022.57313
CGCAGAG132500.022.2421048
CATGGGG120450.018.7331434
GCAGAGT154200.018.1671229
CGAACGT1350.00998527317.4105474
GAGTACA123150.017.0259481
CAGAGTA148650.016.20515410-11
AGAGTAC132000.016.1127210-11
TATACTG41400.015.8967145
TATACCG5359.094947E-1214.9373955
Produced by FastQC (version 0.11.5)