FastQCFastQC Report
Wed 25 May 2016
SRR2049380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6268854
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119220.1901783005314847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114240.18223426482735122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63340.10103920110438049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88750.055.0417061
GTATCAA161800.040.5940741
TCAACGC195600.033.060864
ATCAACG196000.032.9214553
CAACGCA200850.032.1966865
AACGCAG207900.031.0876876
TATCAAC208900.031.023472
ACGCAGA245700.026.0945827
CGCAGAG250350.025.4971648
GTACATG160200.024.4764391
GTGGTAT42350.023.8687531
TACATGG161750.023.0986922
GCAGAGT271900.022.853169
TGGTATC43450.021.8487262
ACATGGG165850.021.5358833
GAGTACT189550.020.5010412-13
CAGAGTA267850.019.85324910-11
AGAGTAC252700.019.78703310-11
GTACTTT208750.018.40428214-15
AGTACTT200800.018.21682712-13
Produced by FastQC (version 0.11.5)