##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049380_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6268854
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869297003886196	31.0	31.0	34.0	30.0	34.0
2	32.05028718167627	33.0	31.0	34.0	30.0	34.0
3	32.02979364330386	34.0	31.0	34.0	30.0	34.0
4	35.710995821564836	37.0	35.0	37.0	33.0	37.0
5	35.804010429976515	37.0	35.0	37.0	35.0	37.0
6	35.85291809316344	37.0	35.0	37.0	35.0	37.0
7	35.78986191096491	37.0	35.0	37.0	35.0	37.0
8	35.794771420741334	37.0	35.0	37.0	35.0	37.0
9	37.5469600982891	39.0	37.0	39.0	35.0	39.0
10-11	37.506835300359526	39.0	37.0	39.0	35.0	39.0
12-13	37.45686229093866	39.0	37.0	39.0	34.5	39.0
14-15	38.75654425832856	40.0	38.0	41.0	35.0	41.0
16-17	38.78367090699513	40.0	38.0	41.0	35.0	41.0
18-19	38.84651843861733	40.0	38.0	41.0	35.0	41.0
20-21	38.87362658310434	40.0	38.0	41.0	35.0	41.0
22-23	38.845138760609196	40.0	38.0	41.0	35.0	41.0
24-25	38.82659956668316	40.0	38.0	41.0	35.0	41.0
26-27	38.71598963702138	40.0	38.0	41.0	34.5	41.0
28-29	38.64091172006877	40.0	38.0	41.0	34.0	41.0
30-31	38.55277623310417	40.0	38.0	41.0	34.0	41.0
32-33	38.432976745031866	40.0	38.0	41.0	34.0	41.0
34-35	38.3441869119938	40.0	38.0	41.0	34.0	41.0
36-37	38.242250337940554	40.0	38.0	41.0	34.0	41.0
38-39	38.16264655389965	40.0	38.0	41.0	34.0	41.0
40-41	38.06363619251621	40.0	38.0	41.0	33.0	41.0
42-43	37.942498102524	40.0	38.0	41.0	33.0	41.0
44-45	37.78476887482146	40.0	37.0	41.0	33.0	41.0
46-47	37.58215504779662	40.0	37.0	41.0	33.0	41.0
48-49	37.400287436906325	40.0	37.0	41.0	32.5	41.0
50-51	37.262780565634486	40.0	37.0	41.0	32.0	41.0
52-53	37.127845296763965	40.0	37.0	41.0	31.5	41.0
54-55	36.992437612999126	40.0	36.0	41.0	31.0	41.0
56-57	36.85464911130487	39.5	36.0	41.0	31.0	41.0
58-59	36.66568873672924	39.0	35.5	41.0	31.0	41.0
60-61	36.48983729721573	39.0	35.0	41.0	31.0	41.0
62-63	36.287813450432886	39.0	35.0	41.0	31.0	41.0
64-65	36.06470464936653	38.5	35.0	40.5	30.5	41.0
66-67	35.79739087877944	38.0	35.0	40.0	30.0	41.0
68-69	35.507568767752446	37.0	35.0	40.0	30.0	41.0
70-71	35.17514564863052	37.0	34.5	39.5	29.0	41.0
72-73	34.87668208575283	36.0	34.0	39.0	29.0	41.0
74-75	34.565516280966186	36.0	34.0	39.0	29.0	41.0
76-77	34.23357323364047	35.5	34.0	38.5	29.0	40.0
78-79	33.894250049530584	35.0	34.0	37.0	29.0	39.0
80-81	33.57274615105089	35.0	34.0	37.0	29.0	39.0
82-83	33.26996776444307	35.0	34.0	36.5	29.0	39.0
84-85	32.99485687495673	35.0	34.0	36.0	29.0	37.0
86-87	32.75840552356141	35.0	33.5	36.0	28.5	37.0
88-89	32.530650099683285	35.0	33.0	35.0	27.5	37.0
90-91	32.33758091989381	35.0	33.0	35.0	27.0	36.0
92-93	32.15747471547431	35.0	33.0	35.0	27.0	36.0
94-95	32.00195697650639	35.0	33.0	35.0	27.0	36.0
96-97	31.80247163197612	35.0	33.0	35.0	26.0	36.0
98-99	31.655459275331665	35.0	33.0	35.0	26.0	35.0
100	31.541743355324595	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19055576688626275
1101	2	0.18226726148535022
1101	3	0.3581143620637377
1101	4	0.2211789896573677
1101	5	0.1894635096784043
1101	6	0.2003042091668874
1101	7	0.09854260575602325
1101	8	0.13901754728052396
1101	9	0.2567664663355913
1101	10-11	0.2324026352804296
1101	12-13	0.18800405171916168
1101	14-15	0.2876336407565816
1101	16-17	0.2280272200946527
1101	18-19	0.31652089116991533
1101	20-21	0.30460564829529346
1101	22-23	0.2552658372287482
1101	24-25	0.27505440628089417
1101	26-27	0.37618978396197633
1101	28-29	0.342796428573827
1101	30-31	0.34488072715868157
1101	32-33	0.36636361599498457
1101	34-35	0.37075579111796486
1101	36-37	0.36813067510156117
1101	38-39	0.4106336526693468
1101	40-41	0.4192963374495804
1101	42-43	0.4333441182102149
1101	44-45	0.4196556035511634
1101	46-47	0.4047263072408356
1101	48-49	0.4917001864936097
1101	50-51	0.39358715653936116
1101	52-53	0.4502584011286075
1101	54-55	0.3976156228591421
1101	56-57	0.4299846401089269
1101	58-59	0.4278009315807694
1101	60-61	0.42461309564539107
1101	62-63	0.35768000588905124
1101	64-65	0.28536990534011153
1101	66-67	0.2951413308169606
1101	68-69	0.30015213513505046
1101	70-71	0.321019880857655
1101	72-73	0.2795617049827541
1101	74-75	0.32567465479562685
1101	76-77	0.2792230552388659
1101	78-79	0.34296952126208424
1101	80-81	0.3790492560893952
1101	82-83	0.35446451838698323
1101	84-85	0.3665360251977319
1101	86-87	0.28070360138124784
1101	88-89	0.3656311117793791
1101	90-91	0.34886138920818155
1101	92-93	0.330500600944049
1101	94-95	0.37183109548725923
1101	96-97	0.34787885927743645
1101	98-99	0.3812545423552507
1101	100	0.41885838494555827
1102	1	0.25187668992138157
1102	2	0.21414565284524656
1102	3	0.40509695512018595
1102	4	0.22863638221958382
1102	5	0.1539170201502742
1102	6	0.12953311350648988
1102	7	0.17465673793442704
1102	8	0.1784117185965144
1102	9	0.1920468544684013
1102	10-11	0.23253429512391222
1102	12-13	0.20755093055083762
1102	14-15	0.27540017971828235
1102	16-17	0.2131063860090947
1102	18-19	0.22411420090497813
1102	20-21	0.22322891993179894
1102	22-23	0.2519454593867678
1102	24-25	0.24192680950833534
1102	26-27	0.25545408625248456
1102	28-29	0.29265631849438734
1102	30-31	0.2628628021338173
1102	32-33	0.26870067688533084
1102	34-35	0.3114166827085256
1102	36-37	0.34922041552232486
1102	38-39	0.33221827731686204
1102	40-41	0.331993173323454
1102	42-43	0.33095024048584065
1102	44-45	0.3508524546627285
1102	46-47	0.32544832426177095
1102	48-49	0.3114230317746589
1102	50-51	0.35759834690045267
1102	52-53	0.36762077631097867
1102	54-55	0.2833175388160427
1102	56-57	0.37043686288446054
1102	58-59	0.34748513810537673
1102	60-61	0.3400271492727356
1102	62-63	0.4089362917056647
1102	64-65	0.39768704955123724
1102	66-67	0.32043790995719235
1102	68-69	0.2953634465730204
1102	70-71	0.3183618235715713
1102	72-73	0.2932098159457581
1102	74-75	0.2656319748991507
1102	76-77	0.2486726210692538
1102	78-79	0.3250996016214529
1102	80-81	0.3259516651478407
1102	82-83	0.3340146218845703
1102	84-85	0.3566179396597491
1102	86-87	0.3400185410715437
1102	88-89	0.3396778812889707
1102	90-91	0.3652169663483136
1102	92-93	0.45589917289282056
1102	94-95	0.44315442332349875
1102	96-97	0.4879651497423936
1102	98-99	0.5159486586650246
1102	100	0.5545847985169132
1103	1	0.18052719938680184
1103	2	0.17849877806759906
1103	3	0.32433665023673086
1103	4	0.17039433111332158
1103	5	0.18095073399270234
1103	6	0.13642797449541888
1103	7	0.13370820898618518
1103	8	0.15347153223212473
1103	9	0.22249148846386646
1103	10-11	0.17541373955798178
1103	12-13	0.17328999688969304
1103	14-15	0.23351144952410863
1103	16-17	0.19783432342810414
1103	18-19	0.22354824200139944
1103	20-21	0.24516830363904774
1103	22-23	0.25830274400099995
1103	24-25	0.2747678195001342
1103	26-27	0.2686234370568741
1103	28-29	0.28047955249345335
1103	30-31	0.22642801565086756
1103	32-33	0.2401861650389634
1103	34-35	0.22512176525009409
1103	36-37	0.24253934322610604
1103	38-39	0.22357695681669298
1103	40-41	0.21000229013654348
1103	42-43	0.24103126492519067
1103	44-45	0.2944365558161621
1103	46-47	0.2747746083229643
1103	48-49	0.22293223084458447
1103	50-51	0.2081720842632393
1103	52-53	0.2687049409507978
1103	54-55	0.2997610175213836
1103	56-57	0.23559006171601737
1103	58-59	0.3191064073956582
1103	60-61	0.2908421128530705
1103	62-63	0.20791720271471092
1103	64-65	0.31169116948368725
1103	66-67	0.3077461686041971
1103	68-69	0.2653576301962772
1103	70-71	0.2580878258063777
1103	72-73	0.29165411762128457
1103	74-75	0.23236858439600638
1103	76-77	0.2861453240311391
1103	78-79	0.2733815654725973
1103	80-81	0.2493296937156586
1103	82-83	0.23870735381957786
1103	84-85	0.20382767003324176
1103	86-87	0.1983702385729771
1103	88-89	0.20718105499236117
1103	90-91	0.2346674965338238
1103	92-93	0.3173905782754858
1103	94-95	0.21088855523753125
1103	96-97	0.27924173703904387
1103	98-99	0.3077486861647749
1103	100	0.3478173951104857
1104	1	0.2172960050352728
1104	2	0.18665697607085718
1104	3	0.3206626083437314
1104	4	0.1743722042065201
1104	5	0.14190546577136587
1104	6	0.1895809138345257
1104	7	0.20789645565694315
1104	8	0.20294613238088743
1104	9	0.2233982304813864
1104	10-11	0.19183338120267024
1104	12-13	0.21329038855765248
1104	14-15	0.27414320625680944
1104	16-17	0.26599166319969214
1104	18-19	0.26878916322408486
1104	20-21	0.31005109669569464
1104	22-23	0.29137491011143624
1104	24-25	0.26327695076587077
1104	26-27	0.28091026962214016
1104	28-29	0.33681392839205415
1104	30-31	0.30216684998318044
1104	32-33	0.3295957949630832
1104	34-35	0.3003855456390454
1104	36-37	0.31219607756988665
1104	38-39	0.32121117184903625
1104	40-41	0.2702879634137645
1104	42-43	0.3024567103768945
1104	44-45	0.30787240588419706
1104	46-47	0.3181533018807272
1104	48-49	0.3317703165385879
1104	50-51	0.2981637094446228
1104	52-53	0.24927339726582431
1104	54-55	0.2926971999546666
1104	56-57	0.2760406873656791
1104	58-59	0.3119713344999937
1104	60-61	0.3313407331750611
1104	62-63	0.4138949090727735
1104	64-65	0.2629346047480965
1104	66-67	0.3417897510571777
1104	68-69	0.3118394696949167
1104	70-71	0.3259599171192775
1104	72-73	0.3227209008142893
1104	74-75	0.31061850759716947
1104	76-77	0.28566843452791346
1104	78-79	0.32133226417630567
1104	80-81	0.30786621349906795
1104	82-83	0.3152900484312866
1104	84-85	0.32128100813698524
1104	86-87	0.3432805134600443
1104	88-89	0.35036161858659653
1104	90-91	0.2728413158268239
1104	92-93	0.39337923291937926
1104	94-95	0.3701171915921293
1104	96-97	0.3516573301385222
1104	98-99	0.40015049353436893
1104	100	0.3790254366061738
1105	1	0.10933944725142553
1105	2	0.1297636801095976
1105	3	0.2493407404167911
1105	4	0.16192681480755766
1105	5	0.1391197611536228
1105	6	0.11870138237656391
1105	7	0.14669202402077275
1105	8	0.11667548186617438
1105	9	0.1585477657743155
1105	10-11	0.18767818182227813
1105	12-13	0.16648633064221485
1105	14-15	0.2281312840388452
1105	16-17	0.23164016173713975
1105	18-19	0.15363624884147242
1105	20-21	0.19177099694910282
1105	22-23	0.16583677010527254
1105	24-25	0.18533180857152587
1105	26-27	0.2309059693897808
1105	28-29	0.18652339247500294
1105	30-31	0.16612850600971285
1105	32-33	0.24372262220936136
1105	34-35	0.25384218049808993
1105	36-37	0.1721229407219269
1105	38-39	0.21213262939751587
1105	40-41	0.23060135400832849
1105	42-43	0.2158026812236642
1105	44-45	0.1813839850235155
1105	46-47	0.2302616752555693
1105	48-49	0.24600211175165043
1105	50-51	0.22522427269515788
1105	52-53	0.21718113918796433
1105	54-55	0.23681088681879459
1105	56-57	0.23199007057606735
1105	58-59	0.20926103668893603
1105	60-61	0.20245571997828193
1105	62-63	0.2175531378846074
1105	64-65	0.21893831657706642
1105	66-67	0.28732808375134766
1105	68-69	0.2592929801884267
1105	70-71	0.3041303162936302
1105	72-73	0.3338699698962273
1105	74-75	0.2680779773197699
1105	76-77	0.28213004825707344
1105	78-79	0.2653190119411235
1105	80-81	0.282158649037882
1105	82-83	0.27965503335462216
1105	84-85	0.2967839473340561
1105	86-87	0.2545711142330447
1105	88-89	0.2710885035452719
1105	90-91	0.2980469515280504
1105	92-93	0.31516898022550777
1105	94-95	0.3137183753544548
1105	96-97	0.2992840953486535
1105	98-99	0.3353439945908647
1105	100	0.3491533438452272
1106	1	0.1892692786794612
1106	2	0.19715783167556822
1106	3	0.2815887235267738
1106	4	0.20679331824124603
1106	5	0.12810625340607373
1106	6	0.10929828910073525
1106	7	0.16504065634286746
1106	8	0.17282086971407296
1106	9	0.2114506145288928
1106	10-11	0.1943783785333295
1106	12-13	0.1642922379363867
1106	14-15	0.26016514011156033
1106	16-17	0.265599639725302
1106	18-19	0.20012860317628167
1106	20-21	0.23946763210152255
1106	22-23	0.21739760680008402
1106	24-25	0.2289333966194107
1106	26-27	0.25524839801092014
1106	28-29	0.24666461033304898
1106	30-31	0.2675445479710845
1106	32-33	0.256793517096952
1106	34-35	0.26847263340395244
1106	36-37	0.25032792638433676
1106	38-39	0.23199493781483937
1106	40-41	0.23638837395075996
1106	42-43	0.27990271926942256
1106	44-45	0.2466581148123197
1106	46-47	0.24623689245279934
1106	48-49	0.25410649360522086
1106	50-51	0.22460173842115694
1106	52-53	0.21709267460087744
1106	54-55	0.15096644130765213
1106	56-57	0.13591569987639218
1106	58-59	0.21644027554953738
1106	60-61	0.17401497918284292
1106	62-63	0.15309582256927
1106	64-65	0.1734564586039795
1106	66-67	0.1737856751787774
1106	68-69	0.22054839712723862
1106	70-71	0.271146632616599
1106	72-73	0.25204653475014993
1106	74-75	0.2228993263768615
1106	76-77	0.17982079602711565
1106	78-79	0.19174675003206687
1106	80-81	0.18045064829720303
1106	82-83	0.24196358060302003
1106	84-85	0.20267863594417435
1106	86-87	0.2511713218572993
1106	88-89	0.2873974074555363
1106	90-91	0.22848628085605327
1106	92-93	0.2872351457877258
1106	94-95	0.29553003915469844
1106	96-97	0.3497304662868359
1106	98-99	0.35934959109141573
1106	100	0.3706671108837618
1107	1	0.09383484723894497
1107	2	0.0686721010750233
1107	3	0.2222052528079388
1107	4	0.18370169554398785
1107	5	0.1528187984378775
1107	6	0.1484856024670691
1107	7	0.10525149818516866
1107	8	0.11392036972507213
1107	9	0.1697351806436913
1107	10-11	0.11002762030982183
1107	12-13	0.14862698795285212
1107	14-15	0.17735886819040303
1107	16-17	0.15358280897950038
1107	18-19	0.16662307363323237
1107	20-21	0.1374026863603035
1107	22-23	0.1584191423904997
1107	24-25	0.146657658210124
1107	26-27	0.2379506631024384
1107	28-29	0.2593385882781334
1107	30-31	0.23020248116071684
1107	32-33	0.277897155053374
1107	34-35	0.19822875046282462
1107	36-37	0.23054978325528452
1107	38-39	0.2606544295619102
1107	40-41	0.2930630586757985
1107	42-43	0.31666414800954357
1107	44-45	0.3098964302332803
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2216	76-77	-0.6205408347647889
2216	78-79	-0.8579645674034069
2216	80-81	-1.0504411239036315
2216	82-83	-0.9119160592692808
2216	84-85	-0.9814684535507396
2216	86-87	-0.8551600284409808
2216	88-89	-1.1811891574405635
2216	90-91	-0.9927616217067232
2216	92-93	-1.0262589227814907
2216	94-95	-1.0206053775071595
2216	96-97	-1.0809642942941906
2216	98-99	-1.164121947259222
2216	100	-1.1330551085660083
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	338.0
3	48.0
4	48.0
5	89.0
6	259.0
7	687.0
8	1635.0
9	2855.0
10	4284.0
11	6087.0
12	8167.0
13	11801.0
14	14337.0
15	18069.0
16	22824.0
17	31428.0
18	31141.0
19	17990.0
20	14077.0
21	14447.0
22	15271.0
23	17228.0
24	20591.0
25	26051.0
26	32950.0
27	43008.0
28	56193.0
29	74267.0
30	97436.0
31	125914.0
32	163007.0
33	212336.0
34	288666.0
35	416093.0
36	678547.0
37	1251228.0
38	1808421.0
39	738225.0
40	2811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4302673568594	20.525834431940282	14.341404749780132	23.702493461420186
2	15.342612860340981	20.46056583866844	40.04264575311532	24.154175547875255
3	17.777587418689286	25.241918219821358	30.88725626725395	26.093238094235403
4	12.461013570595426	17.188883826821133	38.34980525927571	32.00029734330773
5	12.572648844589457	37.91831808493227	35.95027097456728	13.558762095910993
6	30.84216030553591	38.3500397361304	17.176520620834363	13.631279337499327
7	26.612503656968244	32.960043414633674	22.447468069921552	17.979984858476524
8	24.78582468483268	36.722709705968256	20.208674273742094	18.28279133545697
9	24.68267724850507	16.456181624264975	21.56563225112596	37.29550887610399
10-11	23.70833648382942	27.02649479474239	28.9824105011857	20.28275822024249
12-13	24.249419149811033	24.48571707399126	29.326740430005888	21.93812334619182
14-15	21.510444590812053	26.572582851412935	26.74758324622263	25.169389311552386
16-17	20.690960476265715	30.015416694615688	27.486254065436366	21.807368763682227
18-19	20.56220509946719	28.484438376346798	30.196897326160727	20.756459198025283
20-21	21.60254100811624	27.68191712340245	30.00478317356587	20.71075869491544
22-23	21.268249348285988	27.507882620970275	30.076318574335914	21.147549456407823
24-25	21.1853391859779	27.61813296767332	30.007424012741097	21.18910383360768
26-27	20.953501914506003	27.640996964435022	30.094995899965376	21.3105052210936
28-29	20.943867937131834	27.62077308653565	30.24146840739484	21.193890568937675
30-31	21.284711687335516	27.926404092358826	29.779358396287424	21.009525824018233
32-33	21.01350911057199	27.7222186197502	30.107451238813027	21.156821030864783
34-35	21.14955233408876	27.517906397171153	30.141697463122814	21.19084380561727
36-37	21.355194441978547	27.81567016403887	29.6517008423954	21.177434551587176
38-39	21.273059507867092	27.936091793770295	29.699665089286924	21.09118360907569
40-41	21.162250503342204	27.732446471611645	29.81081128721209	21.294491737834065
42-43	21.163190016113077	27.690262650664295	29.755371863385477	21.39117546983715
44-45	21.318263343360975	27.82119048050129	29.526418649962032	21.334127526175703
46-47	21.36337373736729	27.950032589730235	29.207090108769616	21.479503564132855
48-49	21.383497859797387	28.001687404557078	28.975494349077074	21.639320386568464
50-51	21.421825465475727	27.98481406852971	28.8639815238206	21.729378942173962
52-53	21.399820843376098	28.02820117641025	28.882389522807056	21.689588457406593
54-55	21.45962701956626	28.32776942737697	28.66410026436363	21.54850328869314
56-57	21.39520048391886	28.09456389675919	28.715589912901464	21.794645706420482
58-59	21.23093309358692	27.785704099287173	29.046494362758313	21.93686844436759
60-61	21.443941090883918	28.05859071602208	28.74249682118375	21.754971371910255
62-63	21.243653783833558	28.126205126041377	28.767811737966525	21.86232935215854
64-65	21.238831048031457	27.91276488798785	28.8974144932012	21.950989570779488
66-67	21.163198237227	27.95721386344459	28.757108962307456	22.122478937020954
68-69	21.505577460924275	28.038913612466267	28.640898934118926	21.81460999249054
70-71	21.439256958042378	27.91794315521649	28.62878609627801	22.014013790463125
72-73	21.273049808587416	27.984091343965424	28.672533775864345	22.07032507158281
74-75	21.36066525794107	27.99809819651698	28.567761878634723	22.073474666907224
76-77	21.397812566948524	27.963900319821516	28.411848247410315	22.22643886581965
78-79	21.40754669575326	27.98805960625415	28.527347449904383	22.0770462480882
80-81	21.332849659633776	27.90111297593484	28.52247096641738	22.243566398014003
82-83	21.25697265303466	27.89514252862122	28.631003883373722	22.216880934970398
84-85	21.33548902459889	27.81128076855126	28.47821570020152	22.375014506648334
86-87	21.37870972074732	27.936578277674062	28.401358672287547	22.28335332929107
88-89	21.21220242339667	27.765723220777694	28.506871097490045	22.51520325833559
90-91	21.129468528751055	27.671617633968527	28.55352663099086	22.64538720628956
92-93	21.202427046935497	27.79181225800359	28.42270982381002	22.58305087125089
94-95	21.05683674018772	27.591951169417474	28.5803357601064	22.7708763302884
96-97	21.067827916072208	27.6101530943542	28.57564000417634	22.746378985397257
98-99	21.087934990943047	27.670205697704375	28.452006926514656	22.789852384837918
100	21.090275183345494	27.527363439630705	28.454707034845455	22.92765434217835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	470.0
1	343.0
2	397.5
3	571.5
4	387.0
5	283.0
6	723.0
7	1918.5
8	3323.5
9	3877.0
10	3121.0
11	2236.0
12	2235.5
13	2626.0
14	3128.5
15	3561.0
16	4164.5
17	4919.5
18	5720.0
19	6899.5
20	8578.5
21	10811.0
22	13839.0
23	18360.0
24	24382.0
25	32314.0
26	43294.5
27	56957.0
28	73154.0
29	91670.0
30	110552.0
31	127703.5
32	143818.0
33	159276.5
34	173889.5
35	186170.5
36	196947.5
37	208786.5
38	219002.5
39	224812.0
40	228684.5
41	232401.5
42	231952.0
43	230277.5
44	228155.0
45	223744.5
46	219247.0
47	212087.5
48	204427.0
49	198499.0
50	192914.5
51	185958.0
52	173919.0
53	162333.0
54	153598.5
55	144903.0
56	133343.0
57	119168.5
58	107737.5
59	97358.0
60	84531.0
61	70190.5
62	58223.5
63	47540.5
64	37306.0
65	29638.5
66	23370.0
67	17605.5
68	13132.0
69	9848.5
70	6962.5
71	4861.5
72	3492.0
73	2337.5
74	1589.5
75	1026.5
76	624.0
77	373.0
78	217.0
79	113.0
80	68.5
81	37.5
82	25.5
83	19.0
84	8.5
85	4.0
86	2.5
87	2.5
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.041235607018443886
2	0.0
3	0.0
4	9.57112735437769E-5
5	0.0
6	0.0
7	0.0
8	6.540270358824755E-4
9	0.0
10-11	0.0
12-13	0.045040130141809014
14-15	1.75470668163591E-4
16-17	7.97593946198141E-6
18-19	4.785563677188845E-5
20-21	5.5831576233869866E-5
22-23	0.0
24-25	1.1166315246773973E-4
26-27	7.178345515783268E-5
28-29	3.4296539686520057E-4
30-31	0.0
32-33	1.6749472870160957E-4
34-35	7.975939461981409E-5
36-37	7.178345515783268E-5
38-39	3.030856995552935E-4
40-41	1.1963909192972113E-4
42-43	5.26412004490773E-4
44-45	2.472541233214237E-4
46-47	1.2761503139170256E-4
48-49	8.77353340817955E-4
50-51	4.067729125610518E-4
52-53	5.981954596486056E-4
54-55	4.2272479148501465E-4
56-57	5.503398228767171E-4
58-59	1.2761503139170256E-4
60-61	1.75470668163591E-4
62-63	9.491367959757876E-4
64-65	3.987969730990704E-4
66-67	0.001507452558314486
68-69	0.0015393563161624118
70-71	4.6260448879492167E-4
72-73	0.0010448480695195645
74-75	0.0010767518273674902
76-77	7.178345515783268E-4
78-79	0.0034535817870379503
80-81	0.0026320600224538646
82-83	0.0012442465560690997
84-85	0.002815506630079437
86-87	0.0013878134663847651
88-89	0.002105648017963092
90-91	0.0028713382063133073
92-93	0.0026480119013778274
94-95	0.0038523787601370202
96-97	0.003246207361026433
98-99	0.003014905116628972
100	0.001403765345308728
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6268854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.22395410668897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29795390864659	45.34740520521914
2	14.707135490817816	17.714437056797376
3	4.683061468573061	8.460974368864424
4	1.9508065984861112	4.699411482330143
5	0.9757038717176937	2.938037259602256
6	0.5523566118860016	1.9959059546849265
7	0.3715886303314108	1.566497563375241
8	0.24115255856302456	1.161852849568817
9	0.1888702019512179	1.0237059336988101
>10	0.9589876173086854	10.256913547308876
>50	0.05326898020624854	2.189968032052279
>100	0.018397755152185745	1.7877029318558935
>500	4.5101290054200483E-4	0.1969214423891577
>1k	1.857054220262393E-4	0.1860683878944508
>5k	2.6529345794908884E-5	0.10119845124411896
>10k+	5.305869158981777E-5	0.3729995331141776
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11922	0.1901783005314847	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11424	0.18223426482735122	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6334	0.10103920110438049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5951878923962816E-5	0.0
5	0.0	1.5951878923962816E-5	0.0	1.5951878923962816E-5	0.0
6	0.0	1.5951878923962816E-5	0.0	1.5951878923962816E-5	0.0
7	0.0	1.5951878923962816E-5	0.0	3.190375784792563E-5	0.0
8	0.0	1.5951878923962816E-5	0.0	3.190375784792563E-5	0.0
9	0.0	1.5951878923962816E-5	0.0	6.380751569585126E-5	0.0
10-11	0.0	1.5951878923962816E-5	0.0	7.178345515783268E-5	0.0
12-13	0.0	1.5951878923962816E-5	0.0	9.57112735437769E-5	0.0
14-15	0.0	1.5951878923962816E-5	0.0	1.116631524677397E-4	0.0
16-17	0.0	1.5951878923962816E-5	0.0	1.2761503139170253E-4	0.0
18-19	0.0	1.5951878923962816E-5	0.0	1.5154284977764675E-4	0.0
20-21	0.0	1.5951878923962816E-5	0.0	2.1535036547349804E-4	0.0
22-23	0.0	1.5951878923962816E-5	0.0	5.104601255668101E-4	0.0
24-25	0.0	1.5951878923962816E-5	0.0	9.17233038127862E-4	0.0
26-27	0.0	1.5951878923962816E-5	0.0	0.0016111397713202444	0.0
28-29	0.0	1.5951878923962816E-5	0.0	0.004841395253422715	0.0
30-31	0.0	1.5951878923962816E-5	0.0	0.01564081728494554	0.0
32-33	0.0	1.5951878923962816E-5	0.0	0.040382181496011874	0.0
34-35	0.0	1.5951878923962816E-5	0.0	0.07964773146734634	0.0
36-37	0.0	1.5951878923962816E-5	0.0	0.14065569241204212	0.0
38-39	0.0	1.5951878923962816E-5	0.0	0.22004819381660506	0.0
40-41	0.0	1.5951878923962816E-5	0.0	0.2998474681337291	0.0
42-43	0.0	1.5951878923962816E-5	0.0	0.379662694329777	0.0
44-45	0.0	2.3927818385944224E-5	0.0	0.4789392128130596	0.0
46-47	0.0	5.583157623386985E-5	0.0	0.59716656345801	0.0
48-49	0.0	7.975939461981409E-5	0.0	0.7178983590940227	0.0
50-51	0.0	7.975939461981409E-5	0.0	0.8362373728914407	0.0
52-53	0.0	7.975939461981409E-5	0.0	0.951114828962359	0.0
54-55	0.0	7.975939461981409E-5	0.0	1.0803489760648437	0.0
56-57	0.0	1.1166315246773972E-4	0.0	1.2335508212505828	0.0
58-59	0.0	1.1166315246773972E-4	0.0	1.3958930930597522	0.0
60-61	0.0	1.1166315246773972E-4	0.0	1.562957121030415	0.0
62-63	0.0	1.1963909192972113E-4	0.0	1.7274209927364714	0.0
64-65	0.0	1.2761503139170253E-4	0.0	1.8972048160636696	0.0
66-67	0.0	1.2761503139170253E-4	0.0	2.092304271243197	0.0
68-69	0.0	1.2761503139170253E-4	0.0	2.3045200925081364	0.0
70-71	0.0	1.2761503139170253E-4	0.0	2.5271285628920372	0.0
72-73	0.0	1.2761503139170253E-4	0.0	2.754761875136987	0.0
74-75	0.0	1.4356691031566535E-4	0.0	2.9825387542922517	0.0
76-77	7.975939461981408E-6	1.4356691031566535E-4	0.0	3.2267460687391987	0.0
78-79	1.5951878923962816E-5	1.4356691031566535E-4	0.0	3.4978322991730226	0.0
80-81	1.5951878923962816E-5	1.4356691031566535E-4	0.0	3.7864655964232057	0.0
82-83	1.5951878923962816E-5	1.4356691031566535E-4	0.0	4.079031031828146	0.0
84-85	1.5951878923962816E-5	1.4356691031566535E-4	0.0	4.3749304099281945	0.0
86-87	1.5951878923962816E-5	1.4356691031566535E-4	0.0	4.686717859436509	0.0
88	1.5951878923962816E-5	1.4356691031566535E-4	0.0	4.937218190118959	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8875	0.0	55.041706	1
GTATCAA	16180	0.0	40.594074	1
TCAACGC	19560	0.0	33.06086	4
ATCAACG	19600	0.0	32.921455	3
CAACGCA	20085	0.0	32.196686	5
AACGCAG	20790	0.0	31.087687	6
TATCAAC	20890	0.0	31.02347	2
ACGCAGA	24570	0.0	26.094582	7
CGCAGAG	25035	0.0	25.497164	8
GTACATG	16020	0.0	24.476439	1
GTGGTAT	4235	0.0	23.868753	1
TACATGG	16175	0.0	23.098692	2
GCAGAGT	27190	0.0	22.85316	9
TGGTATC	4345	0.0	21.848726	2
ACATGGG	16585	0.0	21.535883	3
GAGTACT	18955	0.0	20.50104	12-13
CAGAGTA	26785	0.0	19.853249	10-11
AGAGTAC	25270	0.0	19.787033	10-11
GTACTTT	20875	0.0	18.404282	14-15
AGTACTT	20080	0.0	18.216827	12-13
>>END_MODULE