FastQCFastQC Report
Wed 25 May 2016
SRR2049379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730313
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44900.043.0399321
GTATCAA75100.036.1253431
TCAACGC80300.032.377924
ATCAACG82250.031.5531373
CAACGCA84150.030.7848365
AACGCAG90850.028.5066726
TATCAAC94200.027.9496572
GTACATG128400.025.4138531
ACGCAGA106950.023.5556287
TACATGG131500.023.3467672
CGCAGAG108250.022.7517138
ACATGGG134150.022.2547323
GCAGAGT124150.018.9675359
GTGGTAT24750.018.617741
CATGGGG99750.018.4761924
GAGTACA98200.017.5245021
AGAGTAC105800.017.19274310-11
CAGAGTA117750.016.46580310-11
TATACTG30050.016.427985
AGTACAT101350.015.6331132
Produced by FastQC (version 0.11.5)