FastQCFastQC Report
Wed 25 May 2016
SRR2049379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730313
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118450.25040626275681965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113900.24078744894893847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64280.13588952781771524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79450.054.8532071
GTATCAA141950.040.2399181
TCAACGC172850.032.3819124
ATCAACG173750.032.2682763
CAACGCA178450.031.392065
TATCAAC182550.030.764242
AACGCAG184150.030.4997926
GTGGTAT36900.029.8130021
TGGTATC39150.026.8891072
ACGCAGA212550.026.2253657
CGCAGAG215150.025.842858
GCAGAGT235050.023.354739
GTACATG121600.021.5733131
GAGTACT162950.021.19334812-13
TACATGG122350.020.7803762
CAGAGTA227150.020.68792710-11
AGAGTAC215600.020.3671310-11
ACATGGG124900.019.7164613
AGTACTT172500.018.92976812-13
GTACTTT182600.018.73659714-15
Produced by FastQC (version 0.11.5)