FastQCFastQC Report
Wed 25 May 2016
SRR2049378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5725987
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56150.043.5407871
GTATCAA93800.034.534971
TCAACGC99350.031.8930994
ATCAACG101800.031.1717173
CAACGCA105300.030.091765
AACGCAG111600.028.3873276
TATCAAC117200.027.3164482
GTACATG160000.025.0064621
ACGCAGA130750.023.7982547
CGCAGAG133800.023.1503818
TACATGG164100.023.1476822
ACATGGG163350.022.534473
GTGGTAT34500.019.8964881
GCAGAGT154000.019.2591659
CATGGGG117800.018.477384
AGAGTAC127850.017.64693610-11
CAGAGTA143500.016.99981710-11
GTATAGG21500.016.8381731
TGGTATC37350.016.6145292
GAGTACA122050.016.525791
Produced by FastQC (version 0.11.5)