##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049378_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5725987 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.944999700488317 31.0 31.0 34.0 28.0 34.0 2 31.13980087625068 31.0 31.0 34.0 28.0 34.0 3 31.20611520773624 31.0 31.0 34.0 28.0 34.0 4 34.89997078232975 37.0 35.0 37.0 32.0 37.0 5 34.917313993203265 37.0 35.0 37.0 32.0 37.0 6 35.02700599914041 37.0 35.0 37.0 32.0 37.0 7 35.005468926143216 37.0 35.0 37.0 32.0 37.0 8 34.96209282347306 37.0 35.0 37.0 32.0 37.0 9 36.61767150362025 39.0 37.0 39.0 32.0 39.0 10-11 36.58022372387502 39.0 37.0 39.0 32.0 39.0 12-13 36.527942169620715 39.0 37.0 39.0 32.0 39.0 14-15 37.778624803025224 40.0 38.0 41.0 32.0 41.0 16-17 37.742675978831244 40.0 38.0 41.0 32.0 41.0 18-19 37.77011063769443 40.0 38.0 41.0 32.0 41.0 20-21 37.842290944775115 40.0 38.0 41.0 32.5 41.0 22-23 37.797710420928304 40.0 38.0 41.0 32.0 41.0 24-25 37.80344427956263 40.0 38.0 41.0 32.0 41.0 26-27 37.79265548454791 40.0 38.0 41.0 32.5 41.0 28-29 37.73609999463848 40.0 38.0 41.0 32.0 41.0 30-31 37.67719835899034 40.0 38.0 41.0 32.0 41.0 32-33 37.63141219147022 40.0 38.0 41.0 32.0 41.0 34-35 37.53658199014423 40.0 38.0 41.0 32.0 41.0 36-37 37.46889566811801 40.0 38.0 41.0 32.0 41.0 38-39 37.37698566203521 40.0 38.0 41.0 32.0 41.0 40-41 37.279056082383704 40.0 37.0 41.0 31.0 41.0 42-43 37.1521434645241 40.0 37.0 41.0 31.0 41.0 44-45 37.0408705957593 40.0 37.0 41.0 31.0 41.0 46-47 36.92566224827266 40.0 37.0 41.0 31.0 41.0 48-49 36.79980019165255 40.0 37.0 41.0 30.0 41.0 50-51 36.661292716871344 40.0 36.0 41.0 30.0 41.0 52-53 36.52274079560432 40.0 36.0 41.0 30.0 41.0 54-55 36.382161101658106 39.0 35.5 41.0 30.0 41.0 56-57 36.25385492492386 39.0 35.0 41.0 30.0 41.0 58-59 36.04453930824502 39.0 35.0 41.0 29.5 41.0 60-61 35.828318593807495 39.0 35.0 41.0 29.0 41.0 62-63 35.615774800047575 38.5 35.0 40.0 29.0 41.0 64-65 35.363290293882955 38.0 35.0 40.0 28.5 41.0 66-67 35.0916287445291 37.5 34.5 40.0 28.0 41.0 68-69 34.79560519435339 37.0 34.0 40.0 28.0 41.0 70-71 34.501827807153596 36.5 34.0 39.5 28.0 41.0 72-73 34.148925678664654 36.0 34.0 39.0 28.0 41.0 74-75 33.79351813058605 36.0 34.0 39.0 27.0 40.5 76-77 33.44570054036099 35.0 34.0 38.0 26.0 40.0 78-79 33.12146368826894 35.0 33.0 37.0 26.0 39.0 80-81 32.75816378905506 35.0 33.0 37.0 26.0 39.0 82-83 32.44585003423864 35.0 33.0 36.0 26.0 39.0 84-85 32.152437562292754 35.0 33.0 36.0 25.5 37.0 86-87 31.834779314029177 35.0 33.0 36.0 25.0 37.0 88-89 31.583277083933304 35.0 33.0 35.0 24.5 36.5 90-91 31.379749814311488 35.0 33.0 35.0 24.0 36.0 92-93 31.146064425224857 35.0 32.0 35.0 23.0 36.0 94-95 30.951189375735574 35.0 32.0 35.0 21.5 36.0 96-97 30.706000729655866 35.0 32.0 35.0 19.0 35.5 98-99 30.503106014735977 35.0 32.0 35.0 9.5 35.0 100 30.387792707178694 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25867301051145475 1101 2 0.3160680751382259 1101 3 0.28566089763353375 1101 4 0.3768515591453294 1101 5 0.32343982041378894 1101 6 0.4624321412785548 1101 7 0.43555344904914506 1101 8 0.42113516171116316 1101 9 0.5630990278126191 1101 10-11 0.5360645391648191 1101 12-13 0.5134645992684312 1101 14-15 0.6627216900014474 1101 16-17 0.6687419716841276 1101 18-19 0.6403232075073504 1101 20-21 0.6031532417161785 1101 22-23 0.6150910269979448 1101 24-25 0.641868852286251 1101 26-27 0.532972114161332 1101 28-29 0.5492895244984837 1101 30-31 0.5868871826521271 1101 32-33 0.5237793470448864 1101 34-35 0.6019057982076887 1101 36-37 0.5978724515223632 1101 38-39 0.6477871250913196 1101 40-41 0.6261449959867704 1101 42-43 0.6427809466729073 1101 44-45 0.5387361298669759 1101 46-47 0.5383080557650786 1101 48-49 0.5248035738814778 1101 50-51 0.5245193821639873 1101 52-53 0.5380444480813651 1101 54-55 0.5334649285068878 1101 56-57 0.49480769675706426 1101 58-59 0.534141659511306 1101 60-61 0.6005972823301065 1101 62-63 0.6129978994516634 1101 64-65 0.5759935263637388 1101 66-67 0.5934400484497928 1101 68-69 0.5932840228774126 1101 70-71 0.5838142605132717 1101 72-73 0.6464559645330539 1101 74-75 0.6637388664770896 1101 76-77 0.6522870517432793 1101 78-79 0.5949763543168416 1101 80-81 0.6079584291590194 1101 82-83 0.6160706140251904 1101 84-85 0.5777008071385339 1101 86-87 0.6066021481343356 1101 88-89 0.5873161410721224 1101 90-91 0.602193500968454 1101 92-93 0.6281880670451443 1101 94-95 0.6119481437120129 1101 96-97 0.627834775064894 1101 98-99 0.7139950799880701 1101 100 0.6590324528870752 1102 1 0.12365198101581143 1102 2 0.1772216381682945 1102 3 0.161548456807445 1102 4 0.2582585434252991 1102 5 0.1040605703459292 1102 6 0.33839167209250576 1102 7 0.448968028466993 1102 8 0.37994573669346465 1102 9 0.47432852606941367 1102 10-11 0.42056342193377105 1102 12-13 0.491404293969957 1102 14-15 0.5215029680131806 1102 16-17 0.5043473443884281 1102 18-19 0.5663162823312291 1102 20-21 0.49744997459643514 1102 22-23 0.5145537005101701 1102 24-25 0.5836953949646997 1102 26-27 0.5086292469055707 1102 28-29 0.5120761485858125 1102 30-31 0.4662499506601776 1102 32-33 0.5036276520470437 1102 34-35 0.5156853173877209 1102 36-37 0.5145539875773721 1102 38-39 0.5002109636566985 1102 40-41 0.49542650080814354 1102 42-43 0.4128020159152612 1102 44-45 0.390962699630208 1102 46-47 0.3702247116874702 1102 48-49 0.4287844432653216 1102 50-51 0.4251271173433224 1102 52-53 0.4571522280905569 1102 54-55 0.4116173773937959 1102 56-57 0.36540751774800384 1102 58-59 0.4637995476648058 1102 60-61 0.4106027781447068 1102 62-63 0.40085528298341444 1102 64-65 0.35192919964789837 1102 66-67 0.41234199115200454 1102 68-69 0.4701831517656174 1102 70-71 0.43331535373793173 1102 72-73 0.4024455410126464 1102 74-75 0.4066269045434794 1102 76-77 0.47198025493161566 1102 78-79 0.47752646835191115 1102 80-81 0.4153627083821263 1102 82-83 0.4094073196534538 1102 84-85 0.4929454836362268 1102 86-87 0.46636243839240166 1102 88-89 0.43778609608740027 1102 90-91 0.4306673155005285 1102 92-93 0.4717649441739873 1102 94-95 0.494240577106261 1102 96-97 0.4753522617981183 1102 98-99 0.4650459451093525 1102 100 0.4837734967092686 1103 1 0.12899774976732203 1103 2 0.21822785701410652 1103 3 0.23794596551036662 1103 4 0.22143166662601033 1103 5 0.26035780818352805 1103 6 0.36521257504712423 1103 7 0.40975647896323864 1103 8 0.3359315889196566 1103 9 0.47762934995679274 1103 10-11 0.4999150169078632 1103 12-13 0.4803250211345542 1103 14-15 0.5631755096298434 1103 16-17 0.5666090451631476 1103 18-19 0.5240510925826527 1103 20-21 0.5024344632886013 1103 22-23 0.5316653894288947 1103 24-25 0.48787456271409724 1103 26-27 0.5165608878743981 1103 28-29 0.5132119596210458 1103 30-31 0.5437222057778328 1103 32-33 0.5774261935135101 1103 34-35 0.5535662295558339 1103 36-37 0.505015001212179 1103 38-39 0.5286887881738878 1103 40-41 0.5659500825656636 1103 42-43 0.5838618341597339 1103 44-45 0.45928743973612995 1103 46-47 0.5021204650288382 1103 48-49 0.5154528574501711 1103 50-51 0.4984754114328922 1103 52-53 0.5166701510265241 1103 54-55 0.515549217516039 1103 56-57 0.5367503339061344 1103 58-59 0.5417752941434202 1103 60-61 0.5364864854325333 1103 62-63 0.5725587956844151 1103 64-65 0.6166991952609138 1103 66-67 0.635300583382886 1103 68-69 0.599812049684104 1103 70-71 0.5452217119229275 1103 72-73 0.615144375199769 1103 74-75 0.670104646080091 1103 76-77 0.6797486249586697 1103 78-79 0.5577241015939123 1103 80-81 0.5252122694981622 1103 82-83 0.5306457886668738 1103 84-85 0.5819641961002944 1103 86-87 0.5674119041451675 1103 88-89 0.5539950319678084 1103 90-91 0.5582775722652116 1103 92-93 0.5790252304896129 1103 94-95 0.6102322705597416 1103 96-97 0.5971523995231642 1103 98-99 0.6170134300555752 1103 100 0.6195131762162518 1104 1 0.025408808017356677 1104 2 0.05834016115263907 1104 3 0.048847278378772785 1104 4 0.16059279676159832 1104 5 0.17001764293316057 1104 6 0.2825664810842312 1104 7 0.32996630018168105 1104 8 0.29061730861350554 1104 9 0.3875867189506579 1104 10-11 0.3386556644656338 1104 12-13 0.31844306064474637 1104 14-15 0.4811344038916019 1104 16-17 0.4875801989029327 1104 18-19 0.46185178729678 1104 20-21 0.435126927680038 1104 22-23 0.4326667043723518 1104 24-25 0.42833640741050516 1104 26-27 0.4082577071890796 1104 28-29 0.42341618688914195 1104 30-31 0.3577763752012828 1104 32-33 0.3967414887850893 1104 34-35 0.4125002136392837 1104 36-37 0.394887485606489 1104 38-39 0.48034347174623804 1104 40-41 0.474805030500427 1104 42-43 0.49769691623217227 1104 44-45 0.46466329326182887 1104 46-47 0.42707043073272644 1104 48-49 0.4211425925404271 1104 50-51 0.45336108032786626 1104 52-53 0.4370427947367048 1104 54-55 0.4560783168669005 1104 56-57 0.43852085498075866 1104 58-59 0.5245108898658728 1104 60-61 0.4325112306916168 1104 62-63 0.4069741418343966 1104 64-65 0.4376572841586679 1104 66-67 0.4401000366116534 1104 68-69 0.42787127426873894 1104 70-71 0.3611384347255253 1104 72-73 0.41194533258445887 1104 74-75 0.4600970060033944 1104 76-77 0.5414298120127157 1104 78-79 0.4912463967432288 1104 80-81 0.4283763110618537 1104 82-83 0.3535624831996529 1104 84-85 0.4345914187050326 1104 86-87 0.4903057876228374 1104 88-89 0.4512864668034169 1104 90-91 0.43846944252084796 1104 92-93 0.4284835022734619 1104 94-95 0.47263857442193924 1104 96-97 0.39591209268519023 1104 98-99 0.45978410780708856 1104 100 0.4470401548981471 1105 1 0.09534804325563684 1105 2 0.15470198850654526 1105 3 0.15450677525711498 1105 4 0.23239294012574874 1105 5 0.21900271782868685 1105 6 0.3155617205698178 1105 7 0.3090675925591029 1105 8 0.2940310864563642 1105 9 0.42942555437772967 1105 10-11 0.39677437335210897 1105 12-13 0.3727925813658217 1105 14-15 0.47992175064539566 1105 16-17 0.4555589627369585 1105 18-19 0.4186452148816642 1105 20-21 0.3695095206743986 1105 22-23 0.4171178293803024 1105 24-25 0.37725211317894747 1105 26-27 0.3533152391443153 1105 28-29 0.4547892649515717 1105 30-31 0.42580321741468197 1105 32-33 0.40831030043329264 1105 34-35 0.4890940172381377 1105 36-37 0.45960835858134175 1105 38-39 0.44253144253185184 1105 40-41 0.517235603549608 1105 42-43 0.5184087491555474 1105 44-45 0.4896745991327407 1105 46-47 0.4579964998693953 1105 48-49 0.5019034309584711 1105 50-51 0.535618706697889 1105 52-53 0.5064484548045627 1105 54-55 0.5237397324287087 1105 56-57 0.5636456598921882 1105 58-59 0.5592019374064705 1105 60-61 0.5487075669991057 1105 62-63 0.5187536157535746 1105 64-65 0.5259791929090412 1105 66-67 0.5209139083467065 1105 68-69 0.5567142179391311 1105 70-71 0.5751671600087818 1105 72-73 0.5119970884566172 1105 74-75 0.5371933680243046 1105 76-77 0.6073579187707239 1105 78-79 0.46883440083918515 1105 80-81 0.5394280769019559 1105 82-83 0.493248175633326 1105 84-85 0.5152268352638316 1105 86-87 0.533176063000262 1105 88-89 0.5565156970257874 1105 90-91 0.5579389409601845 1105 92-93 0.5083945979176008 1105 94-95 0.5047703289237475 1105 96-97 0.5031952026285786 1105 98-99 0.5935251815401443 1105 100 0.600057242561931 1106 1 0.017768149380554377 1106 2 0.03032367133318914 1106 3 0.05717789738151424 1106 4 0.10271429386111208 1106 5 0.011206597588412137 1106 6 0.172227932013314 1106 7 0.16612084186328957 1106 8 0.19417157023642062 1106 9 0.2673206102926713 1106 10-11 0.21394066239594167 1106 12-13 0.20929755786712434 1106 14-15 0.27613519967626843 1106 16-17 0.27914669621364396 1106 18-19 0.2586137873484802 1106 20-21 0.22621726308883439 1106 22-23 0.27724135861835464 1106 24-25 0.2333693527873919 1106 26-27 0.20891511864626722 1106 28-29 0.3019442548940532 1106 30-31 0.2932735854517716 1106 32-33 0.30973574776903234 1106 34-35 0.3539414484475216 1106 36-37 0.3092115540820046 1106 38-39 0.2860576831312258 1106 40-41 0.3710042316123676 1106 42-43 0.3410202136632847 1106 44-45 0.2945893923052978 1106 46-47 0.3044002778772352 1106 48-49 0.2629085894196308 1106 50-51 0.2968440094241913 1106 52-53 0.29467666607762766 1106 54-55 0.2885065259537001 1106 56-57 0.25503910810007113 1106 58-59 0.3276620289745935 1106 60-61 0.3439879580116241 1106 62-63 0.2451373989247685 1106 64-65 0.3455288844714701 1106 66-67 0.3375716454641875 1106 68-69 0.36697595127172633 1106 70-71 0.3449195374343148 1106 72-73 0.3539494558531473 1106 74-75 0.35594867016723697 1106 76-77 0.45838899657363896 1106 78-79 0.32052738392964386 1106 80-81 0.3732494164344615 1106 82-83 0.3342689842761999 1106 84-85 0.3396642970911401 1106 86-87 0.36225501409219163 1106 88-89 0.3479042110464441 1106 90-91 0.370827901427063 1106 92-93 0.32744967937679803 1106 94-95 0.37217183118162467 1106 96-97 0.32520882194915757 1106 98-99 0.34789218488722184 1106 100 0.3537611023894627 1107 1 0.04570006753271372 1107 2 0.020003626011956044 1107 3 0.05456247720748664 1107 4 0.09070887239037972 1107 5 0.03604195282598255 1107 6 0.1523247498063398 1107 7 0.1175071449044296 1107 8 0.15470059003951064 1107 9 0.23508927889848508 1107 10-11 0.19298375475324292 1107 12-13 0.1736875736470438 1107 14-15 0.16978777936206058 1107 16-17 0.19504094882888268 1107 18-19 0.18396741062667132 1107 20-21 0.2033005224294797 1107 22-23 0.19114183400434825 1107 24-25 0.1817275945922674 1107 26-27 0.2018788415405126 1107 28-29 0.2597064266702205 1107 30-31 0.2046703179712992 1107 32-33 0.2608250666768086 1107 34-35 0.31585178828395755 1107 36-37 0.267143936218865 1107 38-39 0.3001274972756036 1107 40-41 0.3314964040364927 1107 42-43 0.29660519333550184 1107 44-45 0.2533974933737966 1107 46-47 0.2532191575869547 1107 48-49 0.256243519646695 1107 50-51 0.28347965165900746 1107 52-53 0.36571764941830764 1107 54-55 0.28939398803019145 1107 56-57 0.2755412494103453 1107 58-59 0.3395154028153371 1107 60-61 0.31334614608508105 1107 62-63 0.2612912494145547 1107 64-65 0.2599909822840871 1107 66-67 0.27150026969030705 1107 68-69 0.2876706057843563 1107 70-71 0.2857901282650772 1107 72-73 0.283497508658364 1107 74-75 0.308402105401143 1107 76-77 0.36521491872561995 1107 78-79 0.2836535241726992 1107 80-81 0.28840068027476917 1107 82-83 0.3048011149688392 1107 84-85 0.3251417651407209 1107 86-87 0.3473329085901611 1107 88-89 0.37201372902453045 1107 90-91 0.32507361457246375 1107 92-93 0.3130420540987764 1107 94-95 0.3555553529763422 1107 96-97 0.2565667412872692 1107 98-99 0.265700517921978 1107 100 0.2734856035139188 1108 1 0.013738640452782391 1108 2 0.037524681371010615 1108 3 0.09079731984400397 1108 4 0.1442053507746408 1108 5 0.14650772056503314 1108 6 0.21510766114708701 1108 7 0.17415901626768715 1108 8 0.1585572244391571 1108 9 0.19902645858089585 1108 10-11 0.1807161882907522 1108 12-13 0.23547566061469638 1108 14-15 0.2647860573336729 1108 16-17 0.2750878039898552 1108 18-19 0.2879806006807115 1108 20-21 0.26222611597481915 1108 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-0.4471780985023486 2215 52-53 -0.4165289934424621 2215 54-55 -0.5493251221290336 2215 56-57 -0.45999996051767056 2215 58-59 -0.42931680174432074 2215 60-61 -0.3637745913868855 2215 62-63 -0.38883036095582924 2215 64-65 -0.33958086515487906 2215 66-67 -0.2969357344204582 2215 68-69 -0.3568895227222697 2215 70-71 -0.3381234353290168 2215 72-73 -0.4009147295115909 2215 74-75 -0.3820314551912247 2215 76-77 -0.3553960424048199 2215 78-79 -0.4170636098359495 2215 80-81 -0.4635524320532909 2215 82-83 -0.40567771369945405 2215 84-85 -0.36944933079031017 2215 86-87 -0.2858916956959945 2215 88-89 -0.2833529081129882 2215 90-91 -0.34548695523503525 2215 92-93 -0.3033430119720464 2215 94-95 -0.35303482165443967 2215 96-97 -0.3387353278947707 2215 98-99 -0.47995505989766585 2215 100 -0.3984396056920332 2216 1 -0.9354338966895632 2216 2 -0.9066363798261712 2216 3 -0.9884823545947476 2216 4 -1.2041168923532055 2216 5 -1.114328422574303 2216 6 -2.619836560554212 2216 7 -2.59605422297858 2216 8 -3.4449605961073857 2216 9 -2.943090721463129 2216 10-11 -2.00589930370559 2216 12-13 -1.7405533502739843 2216 14-15 -2.0683593152310564 2216 16-17 -2.4587935074809977 2216 18-19 -2.011887680230565 2216 20-21 -1.8426110253798242 2216 22-23 -2.007545356784817 2216 24-25 -2.2266918685095476 2216 26-27 -2.272068840367048 2216 28-29 -2.740484230839286 2216 30-31 -2.2886255914495095 2216 32-33 -2.460930589201112 2216 34-35 -3.457582461538685 2216 36-37 -2.53876052416949 2216 38-39 -2.3878767410236534 2216 40-41 -2.969851193013014 2216 42-43 -2.769670723532741 2216 44-45 -2.533081033683928 2216 46-47 -2.3232217989485306 2216 48-49 -2.55231509366471 2216 50-51 -2.8896107665255073 2216 52-53 -3.188245731446152 2216 54-55 -2.554005435822276 2216 56-57 -2.5129938309244224 2216 58-59 -3.6123395624942134 2216 60-61 -4.377617474641411 2216 62-63 -3.449486747847743 2216 64-65 -3.497463935316425 2216 66-67 -3.104076548000883 2216 68-69 -3.4283614715801356 2216 70-71 -3.2634322644903335 2216 72-73 -3.7632026856371006 2216 74-75 -4.187652486496802 2216 76-77 -4.903410851374698 2216 78-79 -3.8516944651159903 2216 80-81 -3.722362394172727 2216 82-83 -3.9507123080126085 2216 84-85 -4.003101840970757 2216 86-87 -3.617784822879994 2216 88-89 -3.5088344768064275 2216 90-91 -3.6932169790536022 2216 92-93 -3.7331030577478472 2216 94-95 -3.552696509006079 2216 96-97 -3.844097612533954 2216 98-99 -3.6414830665603546 2216 100 -3.2377820320051214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67259.0 3 11214.0 4 7544.0 5 6504.0 6 5975.0 7 6420.0 8 7053.0 9 8159.0 10 9226.0 11 10406.0 12 11753.0 13 13356.0 14 14881.0 15 15842.0 16 16348.0 17 17699.0 18 16407.0 19 15324.0 20 15221.0 21 15920.0 22 17975.0 23 20962.0 24 25640.0 25 32558.0 26 39513.0 27 47290.0 28 58819.0 29 75332.0 30 90514.0 31 110235.0 32 141405.0 33 189408.0 34 263879.0 35 385791.0 36 646173.0 37 1166799.0 38 1557049.0 39 562566.0 40 1568.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27808393475887 20.191897192741948 14.18033193518928 23.349686937309905 2 15.461230300396837 20.087222411208188 40.629094392637825 23.822452895757145 3 18.436787577802967 25.054233194349123 30.263310545141447 26.24566868270646 4 12.678875326300046 16.785188860893964 38.691165524543706 31.844770288262286 5 13.270055807723269 37.78337852782165 35.547277907924155 13.399287756530923 6 31.169776507321917 37.64821612970298 17.69083192532888 13.491175437646225 7 27.191138370876473 32.438412180791325 22.4902382721235 17.88021117620871 8 24.83783646367563 36.56125767443031 20.463033353140943 18.137872508753116 9 24.8475029126105 17.055474834136703 21.59325909758346 36.50376315566933 10-11 23.850010378526164 27.032225498466282 29.024194895442463 20.093569227565087 12-13 24.225774330504137 24.564547475153752 29.020945786687015 22.188732407655095 14-15 21.681466722064954 26.77374400749764 26.09648072946824 25.448308540969165 16-17 21.321932246739884 30.111757333422556 26.83309029018679 21.73322012965077 18-19 21.265903753582 29.133097681400937 28.69577121203729 20.905227352979768 20-21 22.28677328512009 28.325024259792414 28.306696191920206 21.08150626316729 22-23 21.999874549961092 28.108234379810305 28.332795191105138 21.559095879123472 24-25 21.8111813125015 28.2960280766736 28.247072980569527 21.64571763025537 26-27 21.560346473823238 28.291914521734284 28.313151130047885 21.83458787439459 28-29 21.630095577443377 28.277850998287175 28.458926526472904 21.633126897796544 30-31 21.857009436499766 28.576683779809937 28.105099025984376 21.461207757705917 32-33 21.59359386117674 28.345015285470744 28.36748376997059 21.693907083381927 34-35 21.754492480028933 28.13814051227442 28.40466018501492 21.702706822681733 36-37 21.95464597276522 28.389721943534376 27.978304423806254 21.677327659894154 38-39 21.79675565692326 28.53204731576066 28.106545808130086 21.564651219185997 40-41 21.723504955447577 28.26122943740188 28.296242057407245 21.719023549743298 42-43 21.630192025832436 28.283761588089572 28.256750884967424 21.829295501110565 44-45 21.71859353812198 28.349757767584943 28.180034779716088 21.751613914576986 46-47 21.762279834260006 28.37351755643491 28.059529494864805 21.80467311444028 48-49 21.680399442605722 28.322745334548383 28.099221562828454 21.897633660017444 50-51 21.72844792832051 28.226803930987664 28.063046264729213 21.98170187596261 52-53 21.702713839460465 28.268585222393178 28.096344650963655 21.932356287182696 54-55 21.77933076798763 28.495376699614322 27.95666283757518 21.768629694822863 56-57 21.711688700584418 28.27893819991415 28.00784335375615 22.001529745745284 58-59 21.64683254295884 27.976776119418627 28.250363593813756 22.12602774380877 60-61 21.810690134056024 28.28239486313378 27.965752128699094 21.941162874111104 62-63 21.669228052499136 28.34754842050009 27.94023959127078 22.042983935729993 64-65 21.70554817528807 28.131488064581728 28.090423922409123 22.072539837721077 66-67 21.624153201516723 28.14167129054395 27.987248930451763 22.246926577487564 68-69 21.88462146577787 28.314850743074377 27.869678739241298 21.93084905190645 70-71 21.86938334028243 28.15845236120389 27.950328843892862 22.02183545462082 72-73 21.70478137426086 28.283011192491088 28.01159125268181 22.000616180566237 74-75 21.800605910148427 28.310593504381853 27.898229736767487 21.990570848702234 76-77 21.86010704821275 28.30103830983568 27.744622759131737 22.09423188281983 78-79 21.899338907660216 28.338123224537288 27.851711956616686 21.910825911185807 80-81 21.841725067705664 28.23916881913444 27.891815596704774 22.02729051645512 82-83 21.788875724882935 28.234853553272686 27.977958894233986 21.998311827610397 84-85 21.873709353591867 28.174867869255028 27.85538156312261 22.096041214030492 86-87 22.009448378138487 28.25983328148876 27.76675516495527 21.96396317541748 88-89 21.895914563863304 28.120730824717636 27.85375818456508 22.12959642685398 90-91 21.87719069131175 28.085740354843402 27.8575000753434 22.17956887850145 92-93 21.967960028522604 28.22948902768165 27.693296934955764 22.10925400883998 94-95 21.93364249283059 28.02730564327168 27.810675026383265 22.228376837514457 96-97 21.969548655978436 27.98521023537078 27.81194752974465 22.233293578906135 98-99 22.01298221599176 28.073099013322945 27.71687221783773 22.19704655284757 100 22.04660960634385 27.91386009084547 27.738274641559613 22.301255661251066 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1433.0 1 1158.0 2 1115.5 3 1428.5 4 1358.5 5 1116.5 6 941.0 7 825.0 8 825.5 9 865.0 10 803.5 11 744.0 12 751.5 13 795.0 14 912.0 15 1058.0 16 1293.5 17 1681.0 18 2259.5 19 3089.0 20 4358.5 21 6471.5 22 9317.5 23 13344.5 24 19077.5 25 26729.0 26 37294.0 27 49732.5 28 64438.5 29 81403.0 30 98396.0 31 114360.0 32 127992.5 33 140964.5 34 154587.0 35 166034.5 36 177436.5 37 189288.0 38 198680.5 39 204413.0 40 208368.0 41 210749.5 42 210267.0 43 210009.5 44 207843.0 45 203270.0 46 199190.0 47 193427.0 48 185776.0 49 180115.5 50 175827.0 51 169705.5 52 160158.5 53 150748.0 54 142747.5 55 134885.5 56 125108.5 57 113006.0 58 102524.5 59 93069.5 60 81731.5 61 69612.0 62 59532.0 63 50141.5 64 40920.0 65 33045.5 66 26313.5 67 20407.5 68 15636.0 69 11985.5 70 8906.5 71 6482.0 72 4813.5 73 3494.5 74 2463.5 75 1746.5 76 1137.0 77 710.5 78 495.0 79 354.5 80 257.5 81 166.5 82 122.5 83 107.5 84 86.0 85 69.0 86 60.5 87 54.0 88 40.5 89 31.0 90 22.5 91 17.0 92 15.0 93 15.0 94 12.0 95 14.0 96 18.5 97 11.5 98 11.0 99 19.0 100 33.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02191761874415712 2 0.042927097110070284 3 0.04462112820025613 4 0.047624977143678464 5 0.05141471679904268 6 0.04814890428497305 7 0.045668982482845316 8 0.04537209043611171 9 0.04538955467415487 10-11 0.04594841029153576 12-13 0.04694387185999549 14-15 0.04514505534155072 16-17 0.04157361866172592 18-19 0.04495294872307604 20-21 0.04409720105896154 22-23 0.045284769245895945 24-25 0.041468833233467 26-27 0.04156488654270434 28-29 0.042062617326934205 30-31 0.042149938517149974 32-33 0.04216740275519312 34-35 0.04181811799433006 36-37 0.03911116109764133 38-39 0.039652552476979075 40-41 0.04080519218782718 42-43 0.04069167464054668 44-45 0.03926833924002971 46-47 0.03846498429004467 48-49 0.03861343031341147 50-51 0.03991451604762637 52-53 0.04028126504653259 54-55 0.03950410645361228 56-57 0.03772275417321066 58-59 0.038831733288950884 60-61 0.03817682436233264 62-63 0.03837766309982891 64-65 0.03519043965695346 66-67 0.03623829393954265 68-69 0.03723375550800238 70-71 0.03881426905090773 72-73 0.03519917177597504 74-75 0.035557188655859676 76-77 0.036980524056376654 78-79 0.037146434317786615 80-81 0.037207559150937645 82-83 0.03572309891726963 84-85 0.03686700650909616 86-87 0.03750445119767125 88-89 0.03806330681505215 90-91 0.038325270385699446 92-93 0.03677095319985882 94-95 0.03822048495744053 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5725987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.84402151170651 #Duplication Level Percentage of deduplicated Percentage of total 1 77.2586607114297 48.55244935714722 2 13.675915818751392 17.188990958117998 3 4.263095081653112 8.03730117053575 4 1.7080452887312574 4.293617394719844 5 0.886704918479065 2.786205148571717 6 0.5072699103620084 1.9127328695418895 7 0.3385267147446837 1.4892066100591568 8 0.2324734973585942 1.1687655575124114 9 0.17781415287429223 1.00571008034861 >10 0.8876047011221971 9.90664415176066 >50 0.04786665616548837 2.0216756929613635 >100 0.015883504551926643 1.553200763230284 >500 1.1123558753749512E-4 0.052166164508820854 >1k 2.7808188800548853E-5 0.03133408098423636 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7464238043153085E-5 0.0 5 0.0 0.0 0.0 1.7464238043153085E-5 0.0 6 0.0 0.0 0.0 1.7464238043153085E-5 0.0 7 0.0 0.0 0.0 1.7464238043153085E-5 0.0 8 0.0 0.0 0.0 1.7464238043153085E-5 0.0 9 0.0 0.0 0.0 5.239271412945925E-5 0.0 10-11 0.0 0.0 0.0 6.11248331510358E-5 0.0 12-13 0.0 3.492847608630617E-5 0.0 6.985695217261234E-5 0.0 14-15 0.0 7.858907119418888E-5 0.0 1.047854282589185E-4 0.0 16-17 0.0 1.047854282589185E-4 0.0 1.222496663020716E-4 0.0 18-19 0.0 1.047854282589185E-4 0.0 1.5717814238837776E-4 0.0 20-21 0.0 1.047854282589185E-4 0.0 2.0957085651783703E-4 0.0 22-23 0.0 1.047854282589185E-4 0.0 4.278738320572506E-4 0.0 24-25 1.7464238043153085E-5 1.047854282589185E-4 0.0 7.858907119418887E-4 0.0 26-27 1.7464238043153085E-5 1.1351754728049506E-4 0.0 0.0017027632092074258 0.0 28-29 1.7464238043153085E-5 1.222496663020716E-4 0.0 0.005300396246096961 0.0 30-31 1.7464238043153085E-5 1.222496663020716E-4 0.0 0.015971045690463495 0.0 32-33 1.7464238043153085E-5 1.3971390434522468E-4 0.0 0.04015028326120894 0.0 34-35 1.7464238043153085E-5 1.3971390434522468E-4 0.0 0.07826598279039054 0.0 36-37 1.7464238043153085E-5 1.3971390434522468E-4 0.0 0.13774917756536995 0.0 38-39 1.7464238043153085E-5 1.4844602336680122E-4 0.0 0.21647796266390407 0.0 40-41 1.7464238043153085E-5 1.5717814238837776E-4 0.0 0.297127813947185 0.0 42-43 1.7464238043153085E-5 1.5717814238837776E-4 0.0 0.3758042063315896 0.0 44-45 1.7464238043153085E-5 1.5717814238837776E-4 0.0 0.4742064555857357 0.0 46-47 1.7464238043153085E-5 1.5717814238837776E-4 0.0 0.5889464995292515 0.0 48-49 1.7464238043153085E-5 1.5717814238837776E-4 0.0 0.7098077589068923 0.0 50-51 1.7464238043153085E-5 1.8337449945310738E-4 0.0 0.8278048832454562 0.0 52-53 1.7464238043153085E-5 1.9210661847468392E-4 0.0 0.941409751716167 0.0 54-55 1.7464238043153085E-5 1.9210661847468392E-4 0.0 1.0671348013888262 0.0 56-57 1.7464238043153085E-5 1.9210661847468392E-4 0.0 1.2143408638545634 0.0 58-59 1.7464238043153085E-5 1.9210661847468392E-4 0.0 1.3750205859705935 0.0 60-61 1.7464238043153085E-5 2.09570856517837E-4 0.0 1.5364512703224789 0.0 62-63 1.7464238043153085E-5 2.2703509456099008E-4 0.0 1.6960132812037472 0.0 64-65 1.7464238043153085E-5 2.2703509456099008E-4 0.0 1.861495668781644 0.0 66-67 1.7464238043153085E-5 2.2703509456099008E-4 0.0 2.0498562081960716 0.0 68-69 1.7464238043153085E-5 2.2703509456099008E-4 0.0 2.26126080970844 0.0 70-71 1.7464238043153085E-5 2.2703509456099008E-4 0.0 2.4811268345527155 0.0 72-73 1.7464238043153085E-5 2.619635706472963E-4 0.0 2.700477664374718 0.0 74-75 1.7464238043153085E-5 2.619635706472963E-4 0.0 2.9239675186129483 0.0 76-77 1.7464238043153085E-5 2.619635706472963E-4 0.0 3.1638126317785913 0.0 78-79 1.7464238043153085E-5 2.619635706472963E-4 0.0 3.4309892774817685 0.0 80-81 1.7464238043153085E-5 2.619635706472963E-4 0.0 3.7181537436253347 0.0 82-83 1.7464238043153085E-5 2.619635706472963E-4 0.0 4.013989203957326 0.0 84-85 1.7464238043153085E-5 2.619635706472963E-4 0.0 4.309204683838786 0.0 86-87 1.7464238043153085E-5 2.619635706472963E-4 0.0 4.6143573151668 0.0 88 1.7464238043153085E-5 2.7942780869044936E-4 0.0 4.8663400737724345 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5615 0.0 43.540787 1 GTATCAA 9380 0.0 34.53497 1 TCAACGC 9935 0.0 31.893099 4 ATCAACG 10180 0.0 31.171717 3 CAACGCA 10530 0.0 30.09176 5 AACGCAG 11160 0.0 28.387327 6 TATCAAC 11720 0.0 27.316448 2 GTACATG 16000 0.0 25.006462 1 ACGCAGA 13075 0.0 23.798254 7 CGCAGAG 13380 0.0 23.150381 8 TACATGG 16410 0.0 23.147682 2 ACATGGG 16335 0.0 22.53447 3 GTGGTAT 3450 0.0 19.896488 1 GCAGAGT 15400 0.0 19.259165 9 CATGGGG 11780 0.0 18.47738 4 AGAGTAC 12785 0.0 17.646936 10-11 CAGAGTA 14350 0.0 16.999817 10-11 GTATAGG 2150 0.0 16.838173 1 TGGTATC 3735 0.0 16.614529 2 GAGTACA 12205 0.0 16.52579 1 >>END_MODULE