FastQCFastQC Report
Wed 25 May 2016
SRR2049378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5725987
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123710.2160500888318468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115770.20218348382558324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67000.11701039488912567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88950.055.1778451
GTATCAA158100.040.1134761
TCAACGC190750.032.743324
ATCAACG192100.032.5621453
CAACGCA196850.031.7286685
TATCAAC204200.030.8167692
AACGCAG204750.030.5770686
ACGCAGA236950.026.084377
GTGGTAT40350.025.7489151
CGCAGAG241050.025.4260548
GTACATG149200.024.6089131
TGGTATC40750.024.2188742
TACATGG154000.022.7046342
GCAGAGT265950.022.6385129
ACATGGG156150.021.0978553
GAGTACT179650.020.96300112-13
CAGAGTA253150.020.64305910-11
AGAGTAC239150.020.464910-11
GTACTTT195800.019.15352214-15
AGTACTT190550.018.30809212-13
Produced by FastQC (version 0.11.5)