##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049378_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5725987 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.913170078800388 33.0 31.0 34.0 30.0 34.0 2 32.083461244323466 33.0 31.0 34.0 30.0 34.0 3 32.01184162660516 34.0 31.0 34.0 30.0 34.0 4 35.73824547628208 37.0 35.0 37.0 33.0 37.0 5 35.81654272005857 37.0 35.0 37.0 35.0 37.0 6 35.850789217649286 37.0 35.0 37.0 35.0 37.0 7 35.82026365061604 37.0 35.0 37.0 35.0 37.0 8 35.81853486569215 37.0 35.0 37.0 35.0 37.0 9 37.5697981850116 39.0 37.0 39.0 35.0 39.0 10-11 37.52801150264574 39.0 37.0 39.0 35.0 39.0 12-13 37.4743343811294 39.0 37.0 39.0 35.0 39.0 14-15 38.78520864612511 40.0 38.0 41.0 35.0 41.0 16-17 38.81019883558939 40.0 38.0 41.0 35.0 41.0 18-19 38.8726331373089 40.0 38.0 41.0 35.0 41.0 20-21 38.89904884520345 40.0 38.0 41.0 35.0 41.0 22-23 38.86970185227455 40.0 38.0 41.0 35.0 41.0 24-25 38.84915639871345 40.0 38.0 41.0 35.0 41.0 26-27 38.74069003300217 40.0 38.0 41.0 35.0 41.0 28-29 38.66399094164902 40.0 38.0 41.0 34.0 41.0 30-31 38.57403553308801 40.0 38.0 41.0 34.0 41.0 32-33 38.451749803134376 40.0 38.0 41.0 34.0 41.0 34-35 38.360672142636716 40.0 38.0 41.0 34.0 41.0 36-37 38.259206578708614 40.0 38.0 41.0 34.0 41.0 38-39 38.176292576284226 40.0 38.0 41.0 34.0 41.0 40-41 38.076779339526965 40.0 38.0 41.0 33.0 41.0 42-43 37.95098687789546 40.0 38.0 41.0 33.0 41.0 44-45 37.785855172217474 40.0 37.0 41.0 33.0 41.0 46-47 37.576001831649286 40.0 37.0 41.0 33.0 41.0 48-49 37.384010127860925 40.0 37.0 41.0 32.5 41.0 50-51 37.24216104577255 40.0 37.0 41.0 32.0 41.0 52-53 37.10537030559098 40.0 36.5 41.0 31.5 41.0 54-55 36.96241329224115 40.0 36.0 41.0 31.0 41.0 56-57 36.82062288999259 39.0 36.0 41.0 31.0 41.0 58-59 36.62580355142266 39.0 35.0 41.0 31.0 41.0 60-61 36.44628445715996 39.0 35.0 41.0 31.0 41.0 62-63 36.23847242405545 39.0 35.0 41.0 31.0 41.0 64-65 36.00575813392521 38.5 35.0 40.5 30.0 41.0 66-67 35.73285601242196 38.0 35.0 40.0 30.0 41.0 68-69 35.439265230605656 37.0 35.0 40.0 30.0 41.0 70-71 35.10851020094876 37.0 34.5 39.5 29.0 41.0 72-73 34.80558784014005 36.0 34.0 39.0 29.0 41.0 74-75 34.4951824899358 36.0 34.0 39.0 29.0 40.5 76-77 34.166366252665256 35.0 34.0 38.5 29.0 40.0 78-79 33.83119154828678 35.0 34.0 37.0 29.0 39.0 80-81 33.51353094235107 35.0 34.0 37.0 29.0 39.0 82-83 33.21667897604378 35.0 34.0 36.0 29.0 39.0 84-85 32.94704249241222 35.0 34.0 36.0 28.0 37.0 86-87 32.71842627306 35.0 33.5 36.0 28.0 37.0 88-89 32.49685792161247 35.0 33.0 35.0 27.5 37.0 90-91 32.309511879785966 35.0 33.0 35.0 27.0 36.0 92-93 32.13189219605284 35.0 33.0 35.0 27.0 36.0 94-95 31.981418138043274 35.0 33.0 35.0 27.0 36.0 96-97 31.787881285794047 35.0 33.0 35.0 26.0 36.0 98-99 31.646446018826097 35.0 33.0 35.0 26.0 35.0 100 31.535942362425903 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15075852204141782 1101 2 0.16332346828256306 1101 3 0.31634022927899963 1101 4 0.1796047601198154 1101 5 0.15014003252589703 1101 6 0.1363440062953174 1101 7 0.03422626692768205 1101 8 0.10264030714105843 1101 9 0.2250263360610134 1101 10-11 0.23455635868477032 1101 12-13 0.16960147660233815 1101 14-15 0.21069639159765075 1101 16-17 0.15577112941549842 1101 18-19 0.2817498114612036 1101 20-21 0.24910888045135948 1101 22-23 0.25361752218129396 1101 24-25 0.2569869813800949 1101 26-27 0.27566711552230316 1101 28-29 0.2710519718268287 1101 30-31 0.26610049305116945 1101 32-33 0.28747595375346435 1101 34-35 0.31030001496564097 1101 36-37 0.3575364444469571 1101 38-39 0.317801433618321 1101 40-41 0.3066672939722892 1101 42-43 0.39912278032090853 1101 44-45 0.36100489319172624 1101 46-47 0.3302041501450219 1101 48-49 0.34012734046742565 1101 50-51 0.29372202425540195 1101 52-53 0.3337159291901912 1101 54-55 0.34389173944356344 1101 56-57 0.3241218073655432 1101 58-59 0.3279656684510712 1101 60-61 0.3288618533772123 1101 62-63 0.29531381356862596 1101 64-65 0.23406757287801128 1101 66-67 0.21348799263417817 1101 68-69 0.26770768421933155 1101 70-71 0.27004485021946323 1101 72-73 0.18845896993259714 1101 74-75 0.2685360345957193 1101 76-77 0.2244338475361829 1101 78-79 0.28449551138544393 1101 80-81 0.35568823084096834 1101 82-83 0.26475735462877736 1101 84-85 0.323958114952994 1101 86-87 0.26736155656035265 1101 88-89 0.2909258013498359 1101 90-91 0.30543036284353775 1101 92-93 0.30713206424328376 1101 94-95 0.3306540426555955 1101 96-97 0.3406124814770344 1101 98-99 0.38775546724289356 1101 100 0.3944388487955379 1102 1 0.18533973118207214 1102 2 0.15489008645190694 1102 3 0.33848605335459325 1102 4 0.12199086844363904 1102 5 0.12112911161732853 1102 6 0.13204165408476598 1102 7 0.11557396684549559 1102 8 0.17344396385144023 1102 9 0.1351285161540403 1102 10-11 0.18367866413861833 1102 12-13 0.16268642717562898 1102 14-15 0.22452766226653154 1102 16-17 0.12531836873382218 1102 18-19 0.19871691419841397 1102 20-21 0.1706587160060664 1102 22-23 0.17581794494065406 1102 24-25 0.18408378762233468 1102 26-27 0.30130704791552176 1102 28-29 0.24346479881045724 1102 30-31 0.231933069375863 1102 32-33 0.25427129526119074 1102 34-35 0.23604544083932666 1102 36-37 0.2467528979904685 1102 38-39 0.25234229271806186 1102 40-41 0.22541367183521288 1102 42-43 0.26575178578812597 1102 44-45 0.24950330096368845 1102 46-47 0.2320811673501595 1102 48-49 0.22600782535960207 1102 50-51 0.22027532980910536 1102 52-53 0.27068714943364114 1102 54-55 0.1975576153248042 1102 56-57 0.24489592482458988 1102 58-59 0.20789820798393066 1102 60-61 0.2618025271780766 1102 62-63 0.24241248869819998 1102 64-65 0.24440773602702848 1102 66-67 0.2210405967771365 1102 68-69 0.30042942805052775 1102 70-71 0.20767880337668032 1102 72-73 0.21020446066785325 1102 74-75 0.1906245331412606 1102 76-77 0.23117059890326175 1102 78-79 0.32312612686379083 1102 80-81 0.3245307339946564 1102 82-83 0.34330064673415706 1102 84-85 0.29973595260948827 1102 86-87 0.2755437344060212 1102 88-89 0.2453224799560374 1102 90-91 0.25604815821712634 1102 92-93 0.30400155918479754 1102 94-95 0.28224001098231355 1102 96-97 0.3091931155413299 1102 98-99 0.30114825564339043 1102 100 0.32233786566451883 1103 1 0.16289550493742766 1103 2 0.12036021847895029 1103 3 0.30341319677544476 1103 4 0.18151414154367274 1103 5 0.16950455248486662 1103 6 0.11062535653015715 1103 7 0.13252043724147455 1103 8 0.1791091055697862 1103 9 0.20432397651709522 1103 10-11 0.19844624611528872 1103 12-13 0.20738197821108173 1103 14-15 0.28431626106690544 1103 16-17 0.226814444358169 1103 18-19 0.25340060014620747 1103 20-21 0.2807476855970421 1103 22-23 0.24141459394950715 1103 24-25 0.26434889658797545 1103 26-27 0.2516215216173734 1103 28-29 0.26725489444425676 1103 30-31 0.26526293192287653 1103 32-33 0.2812864182653314 1103 34-35 0.2903466734155913 1103 36-37 0.2679521425715521 1103 38-39 0.27356487038012034 1103 40-41 0.29192242530230317 1103 42-43 0.35134689340409864 1103 44-45 0.3377398512688359 1103 46-47 0.2817138437272959 1103 48-49 0.30431265239736405 1103 50-51 0.2767782837286603 1103 52-53 0.2818227202419621 1103 54-55 0.26700112323793235 1103 56-57 0.27180084657388903 1103 58-59 0.33769917506019453 1103 60-61 0.3290761191850251 1103 62-63 0.2808909596304048 1103 64-65 0.3303797649752056 1103 66-67 0.2876708732343616 1103 68-69 0.2711023445575691 1103 70-71 0.32649808127580116 1103 72-73 0.317519021594066 1103 74-75 0.26703298467267444 1103 76-77 0.2553037037103252 1103 78-79 0.28781139133732836 1103 80-81 0.30654070121391186 1103 82-83 0.3092882896739084 1103 84-85 0.3356435863463503 1103 86-87 0.29465939320711954 1103 88-89 0.35221362010707224 1103 90-91 0.3432105759365669 1103 92-93 0.34801162234325034 1103 94-95 0.29003999699306604 1103 96-97 0.3738115484328084 1103 98-99 0.4188099656785944 1103 100 0.4375841130283682 1104 1 0.14736050399425338 1104 2 0.19728175705316886 1104 3 0.26344407809989434 1104 4 0.15592198860448292 1104 5 0.1511629747566161 1104 6 0.16461522697565556 1104 7 0.15072439670561266 1104 8 0.16884640102312432 1104 9 0.1695299523917484 1104 10-11 0.15628526340201887 1104 12-13 0.1518082902968274 1104 14-15 0.17879924651417411 1104 16-17 0.25220225940829977 1104 18-19 0.23633670901979542 1104 20-21 0.24651265161941893 1104 22-23 0.2405369286620811 1104 24-25 0.24375903813002964 1104 26-27 0.2576978589037324 1104 28-29 0.27966502665945114 1104 30-31 0.27558235682689514 1104 32-33 0.3024217609911304 1104 34-35 0.2539830173334181 1104 36-37 0.26132241848699067 1104 38-39 0.3235992821762821 1104 40-41 0.34270677493743307 1104 42-43 0.36795249561124876 1104 44-45 0.28652989289485475 1104 46-47 0.4165451247043279 1104 48-49 0.34131914642640027 1104 50-51 0.3158114984324527 1104 52-53 0.32240936418482136 1104 54-55 0.28777759131008196 1104 56-57 0.3404923805924014 1104 58-59 0.39756687961660475 1104 60-61 0.3304708254151407 1104 62-63 0.3617405618974203 1104 64-65 0.31438571594929243 1104 66-67 0.3684001806249313 1104 68-69 0.27382734380383766 1104 70-71 0.3178558870995616 1104 72-73 0.26342266581737306 1104 74-75 0.233340441689748 1104 76-77 0.21382499929438126 1104 78-79 0.22974245596722653 1104 80-81 0.26921173622775996 1104 82-83 0.30910900417978127 1104 84-85 0.27672382392547945 1104 86-87 0.29794295602351895 1104 88-89 0.29788142477185175 1104 90-91 0.2368634703413406 1104 92-93 0.36257718597912003 1104 94-95 0.3379777004714768 1104 96-97 0.36662683246153094 1104 98-99 0.3782748725512839 1104 100 0.38976780340592754 1105 1 0.22724300229317507 1105 2 0.19034203783748183 1105 3 0.2871206476417214 1105 4 0.2175771874615151 1105 5 0.15243809699212107 1105 6 0.16134351648569378 1105 7 0.20902610924625975 1105 8 0.1726833808647541 1105 9 0.2087881556442568 1105 10-11 0.25471001904472246 1105 12-13 0.18350527805280592 1105 14-15 0.3048759414004323 1105 16-17 0.30049451401815475 1105 18-19 0.24549787581857885 1105 20-21 0.25189650105821926 1105 22-23 0.2663337179592631 1105 24-25 0.282985934305799 1105 26-27 0.309900014166125 1105 28-29 0.30562870638582496 1105 30-31 0.23700715108326875 1105 32-33 0.29306254185059544 1105 34-35 0.28987902441820523 1105 36-37 0.24750641680618202 1105 38-39 0.3163020719513341 1105 40-41 0.2815073916336175 1105 42-43 0.3435972834862113 1105 44-45 0.31931546690357493 1105 46-47 0.2800516411731593 1105 48-49 0.31112610805573127 1105 50-51 0.3441475964984164 1105 52-53 0.34614216065581616 1105 54-55 0.33257119541463 1105 56-57 0.3368832117512639 1105 58-59 0.28548793197568045 1105 60-61 0.2967407648052287 1105 62-63 0.36526626132993556 1105 64-65 0.34528957496505797 1105 66-67 0.336048647541098 1105 68-69 0.32803107129117137 1105 70-71 0.3084658447629067 1105 72-73 0.34965798451615626 1105 74-75 0.3267402905447909 1105 76-77 0.28396320624761273 1105 78-79 0.2788654368422385 1105 80-81 0.32646918598135244 1105 82-83 0.3127706256811891 1105 84-85 0.3255017011353445 1105 86-87 0.31319274439832867 1105 88-89 0.34730596005531567 1105 90-91 0.3091209776767272 1105 92-93 0.393485738636997 1105 94-95 0.4157198979689056 1105 96-97 0.4879830298643064 1105 98-99 0.5169360445275935 1105 100 0.4791680401396867 1106 1 0.12557535945347453 1106 2 0.11601208132124441 1106 3 0.22552327694506857 1106 4 0.16987858957119073 1106 5 0.13881054384807356 1106 6 0.13832114234274684 1106 7 0.16519214724357312 1106 8 0.14169744806388707 1106 9 0.16809416261160948 1106 10-11 0.13220459137183838 1106 12-13 0.13746194202070683 1106 14-15 0.1894062952592961 1106 16-17 0.20559494025643232 1106 18-19 0.21149127937515289 1106 20-21 0.18797581221462423 1106 22-23 0.13192261588270782 1106 24-25 0.15341024870610198 1106 26-27 0.18407299231329688 1106 28-29 0.1906291734335568 1106 30-31 0.2588777351965348 1106 32-33 0.16358337141624446 1106 34-35 0.223803999284776 1106 36-37 0.21470094557162867 1106 38-39 0.29350082596598526 1106 40-41 0.2578443830211228 1106 42-43 0.29340580328813815 1106 44-45 0.25417902832709416 1106 46-47 0.30154117779139966 1106 48-49 0.2727615306260276 1106 50-51 0.194215363263055 1106 52-53 0.25339750548764073 1106 54-55 0.3064015500793502 1106 56-57 0.21830747734514233 1106 58-59 0.22886161435961583 1106 60-61 0.25620962270794223 1106 62-63 0.23672248482189673 1106 64-65 0.19952047184957422 1106 66-67 0.21675072937455298 1106 68-69 0.24601284521205713 1106 70-71 0.22240426241163647 1106 72-73 0.32813145487996564 1106 74-75 0.28645615230755084 1106 76-77 0.2788979069781803 1106 78-79 0.3100321582097507 1106 80-81 0.2993051399446074 1106 82-83 0.3138640447846015 1106 84-85 0.27869219398729683 1106 86-87 0.3363885111816032 1106 88-89 0.3087962551408552 1106 90-91 0.3388792402516856 1106 92-93 0.36109398414308913 1106 94-95 0.34707777692485564 1106 96-97 0.35182779433692346 1106 98-99 0.3486611844364269 1106 100 0.3994222977636426 1107 1 0.058981511593117375 1107 2 0.07913829332796496 1107 3 0.17186514249080886 1107 4 0.11690309717525338 1107 5 0.09294788733836867 1107 6 0.07023134830733113 1107 7 0.12218346637164501 1107 8 0.09694955524727789 1107 9 0.11644636322084523 1107 10-11 0.10769523888335897 1107 12-13 0.09309863067411328 1107 14-15 0.145552616827473 1107 16-17 0.1409012245690917 1107 18-19 0.14451586411863104 1107 20-21 0.08399337646248028 1107 22-23 0.09705323918874598 1107 24-25 0.10675265305342663 1107 26-27 0.234270382835156 1107 28-29 0.18097993569022464 1107 30-31 0.17965847348492048 1107 32-33 0.15864003587406472 1107 34-35 0.15893343835988816 1107 36-37 0.16895636638343348 1107 38-39 0.12481891386155297 1107 40-41 0.14227297685859241 1107 42-43 0.14441426803400503 1107 44-45 0.08986671432708704 1107 46-47 0.15256426275260537 1107 48-49 0.15516794839561499 1107 50-51 0.10135347692097696 1107 52-53 0.15605811458786434 1107 54-55 0.14038822197527878 1107 56-57 0.11615888371330385 1107 58-59 0.14350502632878914 1107 60-61 0.1279561681161141 1107 62-63 0.13753541446279627 1107 64-65 0.10709676790980183 1107 66-67 0.0987151025519708 1107 68-69 0.0782879994132557 1107 70-71 0.09210256197033573 1107 72-73 0.14540452623869982 1107 74-75 0.13750605730681542 1107 76-77 0.09178868458810285 1107 78-79 0.055860458191986595 1107 80-81 0.13886197688314184 1107 82-83 0.13162118814613422 1107 84-85 0.12287364446871152 1107 86-87 0.07515141105884737 1107 88-89 0.1166213923549364 1107 90-91 0.19859884161419217 1107 92-93 0.18875835561644294 1107 94-95 0.20155955729209296 1107 96-97 0.21060378834259197 1107 98-99 0.25124899256252675 1107 100 0.2903678998893504 1108 1 0.07005102725506518 1108 2 0.06327778652424598 1108 3 0.1429577466142149 1108 4 0.07504337872656919 1108 5 0.05591050597684699 1108 6 0.06345595638308055 1108 7 0.04938974835182819 1108 8 0.019796120390559224 1108 9 0.09937756112723406 1108 10-11 0.06144377425663805 1108 12-13 0.06962131058094201 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-0.1977858336346543 2215 6 -0.20365377417491004 2215 7 -0.2020984106257515 2215 8 -0.2115767209008439 2215 9 -0.39318601294433364 2215 10-11 -0.3520322098871773 2215 12-13 -0.34196314254811 2215 14-15 -0.36329675747094115 2215 16-17 -0.36294401260543907 2215 18-19 -0.40896663189424487 2215 20-21 -0.396343046838183 2215 22-23 -0.35666095413468923 2215 24-25 -0.3762473936259383 2215 26-27 -0.4255505682078322 2215 28-29 -0.3871067827440342 2215 30-31 -0.37890963323157933 2215 32-33 -0.31929334170418855 2215 34-35 -0.3877555276083271 2215 36-37 -0.3969278036789632 2215 38-39 -0.41293725781953583 2215 40-41 -0.4088748216695848 2215 42-43 -0.39457477862119106 2215 44-45 -0.41277138181162343 2215 46-47 -0.5051251120763283 2215 48-49 -0.42894640881659996 2215 50-51 -0.3685240124227178 2215 52-53 -0.3912813768619827 2215 54-55 -0.33789165659405995 2215 56-57 -0.3836166591582426 2215 58-59 -0.33088436536328203 2215 60-61 -0.438004635340846 2215 62-63 -0.438803320184185 2215 64-65 -0.36186275706075577 2215 66-67 -0.3621798585970808 2215 68-69 -0.40900902357429203 2215 70-71 -0.4337236384056382 2215 72-73 -0.4278973166086999 2215 74-75 -0.37636626940235374 2215 76-77 -0.2992951144049272 2215 78-79 -0.39267817621779955 2215 80-81 -0.45636784550158893 2215 82-83 -0.394159033341424 2215 84-85 -0.4132128355735034 2215 86-87 -0.348916653772541 2215 88-89 -0.4324271150852468 2215 90-91 -0.4514780587656695 2215 92-93 -0.4254428567953994 2215 94-95 -0.4776824674119169 2215 96-97 -0.5289048519047022 2215 98-99 -0.44234704052964346 2215 100 -0.36796303775327743 2216 1 -0.37153662257546927 2216 2 -0.39633573649125964 2216 3 -0.4732474770679076 2216 4 -0.21896822922812476 2216 5 -0.4247294475339345 2216 6 -0.32056423845570237 2216 7 -0.5976119096833443 2216 8 -0.3819615199031645 2216 9 -0.6016966821044889 2216 10-11 -0.37297543904172414 2216 12-13 -0.3852181884935604 2216 14-15 -0.6764176129127648 2216 16-17 -0.45797754438204663 2216 18-19 -0.47589304436667135 2216 20-21 -0.4712175572155104 2216 22-23 -0.4650479480991194 2216 24-25 -0.42523255458044673 2216 26-27 -0.5590885729589701 2216 28-29 -0.5294945554285633 2216 30-31 -0.6744803672526132 2216 32-33 -0.6342509044005666 2216 34-35 -0.6453945592465047 2216 36-37 -0.6563830678222544 2216 38-39 -0.6314573051959869 2216 40-41 -0.6133591518929649 2216 42-43 -0.6173094139497692 2216 44-45 -0.7586303016080649 2216 46-47 -0.7589668511387515 2216 48-49 -0.8235132613586984 2216 50-51 -0.7742936264034412 2216 52-53 -0.660840115834489 2216 54-55 -0.6031146023723934 2216 56-57 -0.6806547231730562 2216 58-59 -0.7341309039159398 2216 60-61 -0.5737205610493064 2216 62-63 -0.5182726848720307 2216 64-65 -0.6510660215178845 2216 66-67 -0.5554222530036483 2216 68-69 -0.5302371291720718 2216 70-71 -0.6116556319082918 2216 72-73 -0.5887092345054086 2216 74-75 -0.5314908983450692 2216 76-77 -0.48885252558714143 2216 78-79 -0.756596185116635 2216 80-81 -0.8443305846021048 2216 82-83 -0.7605948886698997 2216 84-85 -0.7972805135213648 2216 86-87 -0.622098748850135 2216 88-89 -0.975751111790828 2216 90-91 -0.7201170316219958 2216 92-93 -0.8262558316497 2216 94-95 -0.7314563562890513 2216 96-97 -0.8242324704651409 2216 98-99 -0.9387141342751377 2216 100 -0.9256865296348131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 305.0 3 31.0 4 40.0 5 93.0 6 229.0 7 596.0 8 1552.0 9 2639.0 10 3928.0 11 5451.0 12 7680.0 13 10672.0 14 13322.0 15 16742.0 16 21281.0 17 29095.0 18 29559.0 19 17175.0 20 13434.0 21 13152.0 22 14095.0 23 15859.0 24 18993.0 25 23287.0 26 30342.0 27 39170.0 28 51180.0 29 67582.0 30 87647.0 31 113736.0 32 147325.0 33 193450.0 34 262545.0 35 382278.0 36 625496.0 37 1154491.0 38 1645105.0 39 664052.0 40 2378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53053658372952 20.347810469186673 14.57852728623413 23.543125660849675 2 15.40157181635236 20.355180687626433 39.98659095803047 24.256656537990743 3 17.846250786109014 25.164360310283623 30.74001041217872 26.249378491428637 4 12.568259057838135 17.120213483106824 38.167719761508074 32.143807697546976 5 12.806001829902863 37.70778732120768 35.662987009925104 13.823223838964358 6 30.896682091663845 38.01512298229109 17.239316121395316 13.848878804649747 7 26.69620102176271 32.88072431879429 22.306564789616186 18.116509869826807 8 24.974999711837405 36.32703415722221 20.177511651374257 18.52045447956613 9 24.79500913990898 16.555364166911314 21.488487486960764 37.16113920621895 10-11 23.830948271450843 26.988552366605095 28.705452876508453 20.475046485435612 12-13 24.446652914398463 24.47944799433276 29.07137070766873 22.002528383600048 14-15 21.67068039113315 26.48486193233174 26.583535023751836 25.260922652783275 16-17 20.899008328171195 29.82116445601431 27.36792800961651 21.911899206197987 18-19 20.791617788596298 28.311942246423015 30.038082544080204 20.858357420900482 20-21 21.76512219965936 27.486223967872736 29.92196528889609 20.826688543571816 22-23 21.433981600028083 27.303965237783462 30.02014325215897 21.241909910029484 24-25 21.33801550127664 27.496769112012267 29.88950363081953 21.275711755891567 26-27 21.094742938047236 27.46077595228837 29.974579912778303 21.46990119688609 28-29 21.138281097323823 27.44296290914705 30.129207870041864 21.289548123487265 30-31 21.402458650360188 27.724294519006072 29.753342087573724 21.119904743060015 32-33 21.118198021069436 27.510781358237637 30.056436711220098 21.31458390947283 34-35 21.280805744831692 27.33896363444416 29.9876449129289 21.39258570779525 36-37 21.499658399324904 27.584691120338444 29.590608356572424 21.32504212376423 38-39 21.367944764637674 27.690096847354845 29.666452117846593 21.27550627016089 40-41 21.315432788604763 27.49036800919879 29.751093744624274 21.443105457572173 42-43 21.29273389710508 27.52436360867474 29.615253901617862 21.56764859260232 44-45 21.411284273456594 27.621778951300648 29.441724186770834 21.525212588471927 46-47 21.540247863204577 27.750118276676083 29.08634345800309 21.62329040211625 48-49 21.58903047849977 27.70942507585657 28.90435593260004 21.79718851304363 50-51 21.592973751551597 27.789317332516 28.75817712583441 21.85953179009799 52-53 21.518911404999294 27.880747114517863 28.737277546719543 21.8630639337633 54-55 21.59336292779628 28.10759980184977 28.553699786682003 21.745337483671946 56-57 21.53847698768153 27.87066960882508 28.581983267420018 22.008870136073373 58-59 21.436071702292438 27.584877806538493 28.885283684552597 22.093766806616472 60-61 21.595638334621615 27.83652853713374 28.63061122484229 21.937221903402353 62-63 21.43928271633517 27.917534174110063 28.606661741125095 22.036521368429664 64-65 21.417507777737804 27.720103780013755 28.751215080515784 22.111173361732657 66-67 21.356621341575657 27.743501713444225 28.646583398155816 22.253293546824302 68-69 21.65828908334076 27.843644718978894 28.47145918048976 22.026607017190585 70-71 21.66349092470719 27.717961979640616 28.45661560418225 22.161931491469943 72-73 21.471482131060863 27.79707741671546 28.52829519759026 22.20314525463342 74-75 21.528000630814173 27.838124560824884 28.39574542736132 22.23812938099962 76-77 21.57298070639256 27.773964719841537 28.285671273422803 22.367383300343104 78-79 21.61900661829493 27.775714988049693 28.375195060249276 22.230083333406103 80-81 21.52481580823448 27.725353512314204 28.395536294676088 22.354294384775226 82-83 21.437432172211242 27.709609916208777 28.464530803727207 22.388427107852777 84-85 21.47657148685387 27.604078478657566 28.351623171667455 22.567726862821104 86-87 21.497396649709188 27.723947242446634 28.31652750637412 22.46212860147006 88-89 21.40312923844184 27.583635383713133 28.369437915592155 22.64379746225287 90-91 21.3357879490095 27.49322956230746 28.377820331668268 22.793162157014773 92-93 21.400082747785344 27.63907889035534 28.235324013117392 22.72551434874192 94-95 21.248854956499265 27.427156021946413 28.415076413370095 22.908912608184234 96-97 21.249167801297848 27.421733152910093 28.395466761302707 22.933632284489352 98-99 21.22846823425178 27.46056957670731 28.32600991981314 22.984952269227772 100 21.26909678288073 27.311449258884934 28.356560253682435 23.062893704551897 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 576.0 1 424.0 2 462.0 3 695.5 4 498.5 5 330.5 6 817.0 7 2211.5 8 3770.0 9 4390.5 10 3487.0 11 2330.0 12 2263.0 13 2628.0 14 3009.0 15 3389.5 16 3860.5 17 4515.5 18 5350.0 19 6261.5 20 7611.0 21 9534.5 22 12075.0 23 15833.5 24 21287.0 25 28559.5 26 38413.0 27 50233.5 28 64383.0 29 80937.5 30 97559.5 31 112925.5 32 126248.0 33 139160.5 34 152636.0 35 164211.0 36 175186.5 37 186619.5 38 196390.0 39 202314.0 40 205938.0 41 208849.5 42 209231.0 43 209631.0 44 207541.0 45 203085.0 46 199586.0 47 193712.0 48 186022.5 49 180236.0 50 176222.5 51 170023.0 52 159876.0 53 150257.5 54 142350.5 55 134015.5 56 123687.5 57 112069.0 58 102110.5 59 92740.5 60 81325.5 61 68886.0 62 58530.5 63 49195.5 64 39903.5 65 31938.5 66 25257.5 67 19370.5 68 14635.5 69 10989.0 70 7918.0 71 5654.0 72 4109.0 73 2862.5 74 1981.0 75 1287.5 76 761.5 77 438.0 78 272.0 79 172.5 80 93.0 81 46.5 82 28.0 83 14.5 84 11.5 85 7.5 86 2.0 87 3.0 88 3.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04008042630903633 2 0.0 3 0.0 4 1.222496663020716E-4 5 0.0 6 0.0 7 0.0 8 9.256046162871134E-4 9 0.0 10-11 0.0 12-13 0.044839431175795544 14-15 1.4844602336680122E-4 16-17 0.0 18-19 6.985695217261234E-5 20-21 1.659102614099543E-4 22-23 0.0 24-25 1.222496663020716E-4 26-27 6.11248331510358E-5 28-29 3.492847608630617E-4 30-31 0.0 32-33 2.706956896688728E-4 34-35 9.605330923734197E-5 36-37 5.239271412945925E-5 38-39 3.0562416575517895E-4 40-41 2.7942780869044936E-4 42-43 5.064629032514394E-4 44-45 3.143562847767555E-4 46-47 1.047854282589185E-4 48-49 8.557476641145011E-4 50-51 5.15195022273016E-4 52-53 6.549089266182406E-4 54-55 4.97730784229863E-4 56-57 5.588556173808987E-4 58-59 9.605330923734197E-5 60-61 1.5717814238837776E-4 62-63 8.994082592223839E-4 64-65 5.064629032514394E-4 66-67 0.0016241741380132366 68-69 0.001597977780948507 70-71 4.97730784229863E-4 72-73 0.0010391221635676086 74-75 9.343367353086899E-4 76-77 8.557476641145011E-4 78-79 0.003353133704285392 80-81 0.002811742324947647 82-83 0.0015543171858406245 84-85 0.0030649737765733663 86-87 0.001676566852142696 88-89 0.002113172803221523 90-91 0.0030649737765733663 92-93 0.002689492658645575 94-95 0.003877060845579985 96-97 0.003335669466242239 98-99 0.0030300453004870605 100 0.0015368529477974716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5725987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.96016488249234 #Duplication Level Percentage of deduplicated Percentage of total 1 76.33814569591641 46.53585948446787 2 14.183482567038247 17.292548717892142 3 4.370025702464787 7.991924620889485 4 1.8259057047737153 4.452300512915562 5 0.9277580692500077 2.8278142436271603 6 0.5662490780934247 2.071118229908067 7 0.36492040720575336 1.5571925734574281 8 0.24265818602711461 1.1833986424239524 9 0.18808124699239162 1.0318917445164864 >10 0.9187967660800785 9.994392614632588 >50 0.054389445726698356 2.2498137084989285 >100 0.0189273903074193 1.9028748772053659 >500 4.302657505021269E-4 0.2081712558531297 >1k 1.4342148342681593E-4 0.164788012674743 >5k 2.8684296681196363E-5 0.11715616350001189 >10k+ 5.7368593362392725E-5 0.4187545975370574 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12371 0.2160500888318468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11577 0.20218348382558324 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6700 0.11701039488912567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7464238043153085E-5 0.0 5 0.0 0.0 0.0 1.7464238043153085E-5 0.0 6 0.0 0.0 0.0 1.7464238043153085E-5 0.0 7 0.0 0.0 0.0 1.7464238043153085E-5 0.0 8 0.0 0.0 0.0 1.7464238043153085E-5 0.0 9 0.0 0.0 0.0 5.239271412945925E-5 0.0 10-11 0.0 0.0 0.0 7.858907119418888E-5 0.0 12-13 0.0 1.7464238043153085E-5 0.0 1.047854282589185E-4 0.0 14-15 0.0 3.492847608630617E-5 0.0 1.3098178532364814E-4 0.0 16-17 0.0 5.239271412945925E-5 0.0 1.659102614099543E-4 0.0 18-19 0.0 5.239271412945925E-5 0.0 2.09570856517837E-4 0.0 20-21 0.0 5.239271412945925E-5 0.0 2.619635706472963E-4 0.0 22-23 0.0 5.239271412945925E-5 0.0 4.802665461867098E-4 0.0 24-25 0.0 6.985695217261234E-5 0.0 8.382834260713481E-4 0.0 26-27 0.0 6.985695217261234E-5 0.0 0.0017289595662721552 0.0 28-29 0.0 6.985695217261234E-5 0.0 0.0053265926031616905 0.0 30-31 0.0 6.985695217261234E-5 0.0 0.016075831118722413 0.0 32-33 0.0 6.985695217261234E-5 0.0 0.04031619352261889 0.0 34-35 0.0 6.985695217261234E-5 0.0 0.07855414271810257 0.0 36-37 0.0 7.858907119418888E-5 0.0 0.13808099808818985 0.0 38-39 0.0 8.732119021576542E-5 0.0 0.2170630146383497 0.0 40-41 0.0 1.047854282589185E-4 0.0 0.2978351155879327 0.0 42-43 0.0 1.047854282589185E-4 0.0 0.37692191756635146 0.0 44-45 0.0 1.222496663020716E-4 0.0 0.4755512019150585 0.0 46-47 0.0 1.222496663020716E-4 0.0 0.5905881379053078 0.0 48-49 0.0 1.222496663020716E-4 0.0 0.7116851644965314 0.0 50-51 0.0 1.222496663020716E-4 0.0 0.8300403057149798 0.0 52-53 0.0 1.222496663020716E-4 0.0 0.9440206553036183 0.0 54-55 8.732119021576542E-6 1.222496663020716E-4 0.0 1.070103721856162 0.0 56-57 1.7464238043153085E-5 1.3971390434522468E-4 0.0 1.2176590690827624 0.0 58-59 1.7464238043153085E-5 1.5717814238837776E-4 0.0 1.378574558412375 0.0 60-61 1.7464238043153085E-5 1.5717814238837776E-4 0.0 1.5405640983816413 0.0 62-63 1.7464238043153085E-5 1.5717814238837776E-4 0.0 1.7009731248080024 0.0 64-65 1.7464238043153085E-5 1.5717814238837776E-4 0.0 1.867704205405985 0.0 66-67 1.7464238043153085E-5 1.5717814238837776E-4 0.0 2.056911760365505 0.0 68-69 1.7464238043153085E-5 1.5717814238837776E-4 0.0 2.268700575114823 0.0 70-71 1.7464238043153085E-5 1.5717814238837776E-4 0.0 2.4890206701482205 0.0 72-73 1.7464238043153085E-5 1.7464238043153084E-4 0.0 2.709384425776727 0.0 74-75 1.7464238043153085E-5 1.7464238043153084E-4 0.0 2.9339657948926536 0.0 76-77 1.7464238043153085E-5 1.7464238043153084E-4 0.0 3.175155654387619 0.0 78-79 1.7464238043153085E-5 1.7464238043153084E-4 0.0 3.4433364937782778 0.0 80-81 1.7464238043153085E-5 1.7464238043153084E-4 0.0 3.732683989677238 0.0 82-83 1.7464238043153085E-5 1.7464238043153084E-4 0.0 4.030309534408653 0.0 84-85 1.7464238043153085E-5 1.7464238043153084E-4 0.0 4.3267649751911765 0.0 86-87 1.7464238043153085E-5 1.7464238043153084E-4 0.0 4.633751351513721 0.0 88 1.7464238043153085E-5 1.7464238043153084E-4 0.0 4.8873844806144335 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8895 0.0 55.177845 1 GTATCAA 15810 0.0 40.113476 1 TCAACGC 19075 0.0 32.74332 4 ATCAACG 19210 0.0 32.562145 3 CAACGCA 19685 0.0 31.728668 5 TATCAAC 20420 0.0 30.816769 2 AACGCAG 20475 0.0 30.577068 6 ACGCAGA 23695 0.0 26.08437 7 GTGGTAT 4035 0.0 25.748915 1 CGCAGAG 24105 0.0 25.426054 8 GTACATG 14920 0.0 24.608913 1 TGGTATC 4075 0.0 24.218874 2 TACATGG 15400 0.0 22.704634 2 GCAGAGT 26595 0.0 22.638512 9 ACATGGG 15615 0.0 21.097855 3 GAGTACT 17965 0.0 20.963001 12-13 CAGAGTA 25315 0.0 20.643059 10-11 AGAGTAC 23915 0.0 20.4649 10-11 GTACTTT 19580 0.0 19.153522 14-15 AGTACTT 19055 0.0 18.308092 12-13 >>END_MODULE