FastQCFastQC Report
Wed 25 May 2016
SRR2049377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5965015
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55700.043.725481
GTATCAA92700.034.388241
TCAACGC99650.030.7592474
ATCAACG101200.030.4274923
CAACGCA106250.028.8930425
AACGCAG112700.027.2765856
TATCAAC115350.027.0617492
GTACATG156700.025.7741411
TACATGG160350.023.8649672
ACGCAGA132850.022.855987
ACATGGG163050.022.5183013
CGCAGAG134800.022.1417888
CATGGGG119250.018.4104274
GCAGAGT154850.018.3945989
GAGTACA119550.017.8945921
GTACGAC5050.017.6875463
GTGGTAT36100.017.45251
AGAGTAC130250.016.96090510-11
CAGAGTA146800.016.37753310-11
TACGTCG2658.766288E-615.9663515
Produced by FastQC (version 0.11.5)