##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049377_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5965015
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96817711271472	31.0	31.0	34.0	28.0	34.0
2	31.151831135378536	31.0	31.0	34.0	28.0	34.0
3	31.217961396576538	31.0	31.0	34.0	28.0	34.0
4	34.891078060993976	37.0	35.0	37.0	32.0	37.0
5	34.905269475433	37.0	35.0	37.0	32.0	37.0
6	35.00649973218844	37.0	35.0	37.0	32.0	37.0
7	34.97608840883049	37.0	35.0	37.0	32.0	37.0
8	34.93613444392009	37.0	35.0	37.0	32.0	37.0
9	36.57906258408403	39.0	37.0	39.0	32.0	39.0
10-11	36.535721452502635	39.0	37.0	39.0	32.0	39.0
12-13	36.47807021440851	39.0	37.0	39.0	32.0	39.0
14-15	37.726333797987095	40.0	38.0	41.0	32.0	41.0
16-17	37.694192554419395	40.0	38.0	41.0	32.0	41.0
18-19	37.725951485453095	40.0	38.0	41.0	32.0	41.0
20-21	37.79150957709243	40.0	38.0	41.0	32.0	41.0
22-23	37.74943382372048	40.0	38.0	41.0	32.0	41.0
24-25	37.754505562852735	40.0	38.0	41.0	32.0	41.0
26-27	37.74092085267179	40.0	38.0	41.0	32.0	41.0
28-29	37.68520095925995	40.0	38.0	41.0	32.0	41.0
30-31	37.628807639209626	40.0	38.0	41.0	32.0	41.0
32-33	37.577382538015414	40.0	38.0	41.0	32.0	41.0
34-35	37.48078638528151	40.0	38.0	41.0	32.0	41.0
36-37	37.413819412021596	40.0	38.0	41.0	32.0	41.0
38-39	37.32088108747422	40.0	37.0	41.0	31.5	41.0
40-41	37.22307932167815	40.0	37.0	41.0	31.0	41.0
42-43	37.10018181010442	40.0	37.0	41.0	31.0	41.0
44-45	36.996795397832194	40.0	37.0	41.0	31.0	41.0
46-47	36.88585452006407	40.0	37.0	41.0	31.0	41.0
48-49	36.76033379631066	40.0	36.5	41.0	30.0	41.0
50-51	36.62647185296265	40.0	36.0	41.0	30.0	41.0
52-53	36.48724194323066	40.0	36.0	41.0	30.0	41.0
54-55	36.34816702053557	39.0	35.5	41.0	30.0	41.0
56-57	36.22205593783083	39.0	35.0	41.0	30.0	41.0
58-59	36.013229053070276	39.0	35.0	41.0	29.0	41.0
60-61	35.798107129655165	39.0	35.0	41.0	29.0	41.0
62-63	35.58659248970874	38.5	35.0	40.0	29.0	41.0
64-65	35.339994115689564	38.0	35.0	40.0	28.0	41.0
66-67	35.07453107829569	37.5	34.0	40.0	28.0	41.0
68-69	34.78445376918583	37.0	34.0	40.0	28.0	41.0
70-71	34.49270144333249	36.5	34.0	39.5	28.0	41.0
72-73	34.14254406736613	36.0	34.0	39.0	27.5	41.0
74-75	33.78822919975893	36.0	34.0	39.0	27.0	40.5
76-77	33.4522142861334	35.0	34.0	38.5	26.0	40.0
78-79	33.13589488039845	35.0	33.0	37.0	26.0	39.0
80-81	32.782529968491275	35.0	33.0	37.0	26.0	39.0
82-83	32.47481028966398	35.0	33.0	36.0	26.0	39.0
84-85	32.188940094869835	35.0	33.0	36.0	26.0	37.0
86-87	31.87874892183842	35.0	33.0	36.0	25.0	37.0
88-89	31.639135609885308	35.0	33.0	35.0	25.0	37.0
90-91	31.443268960765398	35.0	33.0	35.0	24.5	36.0
92-93	31.217151088471695	35.0	32.0	35.0	24.0	36.0
94-95	31.02357102203431	35.0	32.0	35.0	22.5	36.0
96-97	30.7803063361953	35.0	32.0	35.0	19.5	35.5
98-99	30.57852335660514	35.0	32.0	35.0	18.0	35.0
100	30.45716330973183	35.0	32.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3076612686432867
1101	2	0.35873165731014467
1101	3	0.30266590907123003
1101	4	0.40128422207311587
1101	5	0.33438950855819627
1101	6	0.443485245096241
1101	7	0.45224912666810724
1101	8	0.4732509676005492
1101	9	0.5971309458583463
1101	10-11	0.6053098804041355
1101	12-13	0.6091456810334108
1101	14-15	0.7580998218895232
1101	16-17	0.7642324725445135
1101	18-19	0.6678701248007712
1101	20-21	0.6506301095163209
1101	22-23	0.6332621511314969
1101	24-25	0.6649720560106047
1101	26-27	0.5926310503166263
1101	28-29	0.6545069257579783
1101	30-31	0.6644569143102927
1101	32-33	0.6062977199495094
1101	34-35	0.6296936458444264
1101	36-37	0.6394532389460252
1101	38-39	0.6802439953967436
1101	40-41	0.6656021857217524
1101	42-43	0.6545811792939986
1101	44-45	0.6283128477207995
1101	46-47	0.5869418577338195
1101	48-49	0.5847917665915006
1101	50-51	0.6229355312567222
1101	52-53	0.5999857862070641
1101	54-55	0.6024221740569331
1101	56-57	0.6197033730397408
1101	58-59	0.700868042948187
1101	60-61	0.6701840056879078
1101	62-63	0.6907774191210834
1101	64-65	0.6650024558545198
1101	66-67	0.632089700821119
1101	68-69	0.6322580459285021
1101	70-71	0.7046977144892352
1101	72-73	0.7874487614811869
1101	74-75	0.7054017126934653
1101	76-77	0.7058908234680459
1101	78-79	0.6519401116000765
1101	80-81	0.6558603834522572
1101	82-83	0.6710060007808778
1101	84-85	0.6196090576985043
1101	86-87	0.6507566657647672
1101	88-89	0.6683020415092997
1101	90-91	0.6850149170894753
1101	92-93	0.6355420555674343
1101	94-95	0.6621921386541452
1101	96-97	0.6520692950999489
1101	98-99	0.7537441198982684
1101	100	0.7269022751391283
1102	1	-0.031954981140934535
1102	2	0.05239568548158502
1102	3	0.08074227223883312
1102	4	0.12390508239691655
1102	5	0.049513874477050024
1102	6	0.2351551245745469
1102	7	0.3420771097841566
1102	8	0.22959323619299
1102	9	0.3692483252686287
1102	10-11	0.2893377336596501
1102	12-13	0.30682013819215115
1102	14-15	0.431973515199509
1102	16-17	0.3383072534178737
1102	18-19	0.3669920793638539
1102	20-21	0.3292224579148524
1102	22-23	0.29163290696767774
1102	24-25	0.392760998298499
1102	26-27	0.33977177681896364
1102	28-29	0.32279866798705115
1102	30-31	0.3290536679159146
1102	32-33	0.37038879211964115
1102	34-35	0.38964425750775433
1102	36-37	0.305060968844451
1102	38-39	0.37890046522126397
1102	40-41	0.4194952584934626
1102	42-43	0.4159474848718645
1102	44-45	0.3785729404965821
1102	46-47	0.3159213493151043
1102	48-49	0.31849616542005066
1102	50-51	0.4052008333222261
1102	52-53	0.4296565167521109
1102	54-55	0.4035395931658172
1102	56-57	0.34517243734359937
1102	58-59	0.44807309011295615
1102	60-61	0.4176909555458934
1102	62-63	0.4637551134111959
1102	64-65	0.4334066997692929
1102	66-67	0.5005606740777822
1102	68-69	0.4621930398332026
1102	70-71	0.4596489855657353
1102	72-73	0.5074914575547567
1102	74-75	0.4499243681822662
1102	76-77	0.4392135729845634
1102	78-79	0.4287182337132762
1102	80-81	0.3861511556674557
1102	82-83	0.4030577630049663
1102	84-85	0.45958492927247363
1102	86-87	0.4355840147439096
1102	88-89	0.40134273020131417
1102	90-91	0.47881159361735115
1102	92-93	0.46851876557001404
1102	94-95	0.43989413802164634
1102	96-97	0.45184421738658287
1102	98-99	0.5000926584914822
1102	100	0.4571190250663122
1103	1	0.04733455514477214
1103	2	0.16302628494613458
1103	3	0.1723415360756526
1103	4	0.17767919263285137
1103	5	0.1715857757987962
1103	6	0.30174363783576297
1103	7	0.33240620620641437
1103	8	0.32405630049913015
1103	9	0.4471408953823399
1103	10-11	0.39110469232110745
1103	12-13	0.3725980622991756
1103	14-15	0.40452866161823664
1103	16-17	0.41202588247389116
1103	18-19	0.448209273830237
1103	20-21	0.3951477901777096
1103	22-23	0.4588577637139579
1103	24-25	0.42690028272773617
1103	26-27	0.3663289824789615
1103	28-29	0.3958965521548734
1103	30-31	0.4207483068395348
1103	32-33	0.43676365273017836
1103	34-35	0.4572600133928333
1103	36-37	0.40578764830517144
1103	38-39	0.45402101463159283
1103	40-41	0.4979711764963639
1103	42-43	0.5246988072846648
1103	44-45	0.3743380063773003
1103	46-47	0.42014469064779547
1103	48-49	0.4365980398278495
1103	50-51	0.43024387101166184
1103	52-53	0.4698592249063225
1103	54-55	0.4511896263305246
1103	56-57	0.39066352278795335
1103	58-59	0.4891467718262774
1103	60-61	0.49073489574579554
1103	62-63	0.4650670941244215
1103	64-65	0.46813155439235743
1103	66-67	0.5173738948936162
1103	68-69	0.5139695812987171
1103	70-71	0.48720038237620855
1103	72-73	0.49629735523985374
1103	74-75	0.48811097892424016
1103	76-77	0.539219149978365
1103	78-79	0.38962414416601376
1103	80-81	0.36623061508972654
1103	82-83	0.4217876437167405
1103	84-85	0.5036844981453825
1103	86-87	0.4765262973876503
1103	88-89	0.4440350989770607
1103	90-91	0.4365957039410482
1103	92-93	0.3856107794564423
1103	94-95	0.4322805081885228
1103	96-97	0.39907528243103485
1103	98-99	0.4459106350392332
1103	100	0.46937354887172233
1104	1	0.06649243729459542
1104	2	0.03915478971421393
1104	3	0.03729637651156992
1104	4	0.1761083900899294
1104	5	0.17468298360834922
1104	6	0.21707256972177902
1104	7	0.2588376053378738
1104	8	0.2780864188579599
1104	9	0.31727205462320285
1104	10-11	0.3026055430536374
1104	12-13	0.26555789740177005
1104	14-15	0.399108574858964
1104	16-17	0.4104392718098637
1104	18-19	0.35945338722227405
1104	20-21	0.3767283558162333
1104	22-23	0.3789265219103015
1104	24-25	0.3888588128732948
1104	26-27	0.33873716500998796
1104	28-29	0.38591401293096084
1104	30-31	0.3278192646114846
1104	32-33	0.3180930293423785
1104	34-35	0.3677573107826291
1104	36-37	0.3474773284569679
1104	38-39	0.4019044242627672
1104	40-41	0.42188191353905324
1104	42-43	0.39357067141759217
1104	44-45	0.315123139112508
1104	46-47	0.2803981420516948
1104	48-49	0.2231252262174621
1104	50-51	0.2985324222026122
1104	52-53	0.3299633265634938
1104	54-55	0.31395033991799437
1104	56-57	0.3508238287061758
1104	58-59	0.37051106084210517
1104	60-61	0.31095509439697366
1104	62-63	0.29494994289690624
1104	64-65	0.36120308227914677
1104	66-67	0.43129169298675407
1104	68-69	0.4161518877087005
1104	70-71	0.3790237817427311
1104	72-73	0.42677645405155573
1104	74-75	0.4385086063122685
1104	76-77	0.430867126288625
1104	78-79	0.36943046010639335
1104	80-81	0.4297934959746357
1104	82-83	0.37990294381999945
1104	84-85	0.41958339688304847
1104	86-87	0.4089871695299081
1104	88-89	0.3566717665745429
1104	90-91	0.3991191557932261
1104	92-93	0.3678408907009505
1104	94-95	0.4082353873173403
1104	96-97	0.39582602067701345
1104	98-99	0.3554508213280556
1104	100	0.39669862481393636
1105	1	0.08578603953547415
1105	2	0.17989445677045524
1105	3	0.17183431932834026
1105	4	0.2552724148474894
1105	5	0.23723322441992423
1105	6	0.3256502802896861
1105	7	0.33117499521150506
1105	8	0.35851863707182474
1105	9	0.43251413400926
1105	10-11	0.4030743185402059
1105	12-13	0.37837297493334177
1105	14-15	0.4430903230485441
1105	16-17	0.4163445489233055
1105	18-19	0.4043759060109693
1105	20-21	0.3999959349483646
1105	22-23	0.3936076309334666
1105	24-25	0.37479210510093
1105	26-27	0.35046465905355717
1105	28-29	0.4423049910507544
1105	30-31	0.4620190953100334
1105	32-33	0.4448692324983554
1105	34-35	0.4937670091285966
1105	36-37	0.47247903719701156
1105	38-39	0.42149889322799083
1105	40-41	0.4897027308954449
1105	42-43	0.4037108872851576
1105	44-45	0.41986312232420175
1105	46-47	0.41853078570842683
1105	48-49	0.4440208480503216
1105	50-51	0.43940597482271215
1105	52-53	0.44825279606882873
1105	54-55	0.4877587519758535
1105	56-57	0.4749599771043691
1105	58-59	0.45591853906967117
1105	60-61	0.4418555536343476
1105	62-63	0.38515100224378784
1105	64-65	0.44556134272936276
1105	66-67	0.4633783749794915
1105	68-69	0.45996201672910075
1105	70-71	0.438115018799607
1105	72-73	0.4547766007205567
1105	74-75	0.46470834481594636
1105	76-77	0.48765951699427035
1105	78-79	0.33613356618980816
1105	80-81	0.4006820772846069
1105	82-83	0.36777880367597504
1105	84-85	0.3808707477562976
1105	86-87	0.40745376163212654
1105	88-89	0.3701679093444241
1105	90-91	0.3789915342391126
1105	92-93	0.4267080644017476
1105	94-95	0.36012617112898937
1105	96-97	0.38192002440242945
1105	98-99	0.3977723679695657
1105	100	0.3632944271616907
1106	1	0.10244107068752584
1106	2	0.05766963154710325
1106	3	0.08732688092903373
1106	4	0.1373817358504965
1106	5	0.09198152991928765
1106	6	0.1974724755548607
1106	7	0.21853976222277538
1106	8	0.19690860708564628
1106	9	0.2856109497877384
1106	10-11	0.26462325211840465
1106	12-13	0.273008279208689
1106	14-15	0.3588839112759885
1106	16-17	0.40027603930528954
1106	18-19	0.31747620945695587
1106	20-21	0.2933822550081189
1106	22-23	0.3522211242939548
1106	24-25	0.33497086722687897
1106	26-27	0.304297046113561
1106	28-29	0.3591375368130798
1106	30-31	0.32229091832171264
1106	32-33	0.3391199524831592
1106	34-35	0.3900933542501761
1106	36-37	0.2916629160288906
1106	38-39	0.3704524038047907
1106	40-41	0.3646695517575722
1106	42-43	0.3918049780433748
1106	44-45	0.3289889732583262
1106	46-47	0.24600539184493186
1106	48-49	0.2946808017618139
1106	50-51	0.35824783722720355
1106	52-53	0.332899586847212
1106	54-55	0.38889980567557814
1106	56-57	0.33816117201131846
1106	58-59	0.3807588311775021
1106	60-61	0.3698425123663682
1106	62-63	0.28369482389504697
1106	64-65	0.3649364608351462
1106	66-67	0.37406207728740526
1106	68-69	0.38872192740976885
1106	70-71	0.3372398860581569
1106	72-73	0.38319476379205497
1106	74-75	0.3551036639141216
1106	76-77	0.39859979136010537
1106	78-79	0.29788695593779124
1106	80-81	0.3500270419509448
1106	82-83	0.3574826426590363
1106	84-85	0.39787576529817414
1106	86-87	0.38135372760172714
1106	88-89	0.33450777841202495
1106	90-91	0.38314081815715184
1106	92-93	0.3537312351385573
1106	94-95	0.3390559500349646
1106	96-97	0.28605556374437313
1106	98-99	0.2317522506095031
1106	100	0.2165971875663999
1107	1	0.11746446337726013
1107	2	0.13470012525277042
1107	3	0.1298474554705038
1107	4	0.19198794337265923
1107	5	0.17879097281004164
1107	6	0.2446492865839005
1107	7	0.22624150866788995
1107	8	0.3064804224336086
1107	9	0.3490107119279813
1107	10-11	0.3013491379909041
1107	12-13	0.2899250561824829
1107	14-15	0.3125441816785326
1107	16-17	0.3565313410197106
1107	18-19	0.3499054036474192
1107	20-21	0.34299519944959656
1107	22-23	0.3340288772350135
1107	24-25	0.392963284056286
1107	26-27	0.3631113536125383
1107	28-29	0.45503188627348123
1107	30-31	0.39858442224370094
1107	32-33	0.47596501388755996
1107	34-35	0.4645556638924049
1107	36-37	0.4469749466837172
1107	38-39	0.45084786503871044
1107	40-41	0.5029565193288903
1107	42-43	0.4327442261701293
1107	44-45	0.41816353892054536
1107	46-47	0.4860533173443713
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2216	80-81	-3.412067975070684
2216	82-83	-3.666120908998863
2216	84-85	-3.6755542554488905
2216	86-87	-3.312785943621275
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2216	90-91	-3.508522421958162
2216	92-93	-3.594669584718922
2216	94-95	-3.431661525124607
2216	96-97	-3.734119679444184
2216	98-99	-3.474020419898352
2216	100	-3.0867898440394654
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71044.0
3	12117.0
4	8118.0
5	6855.0
6	6515.0
7	6755.0
8	7470.0
9	8601.0
10	9788.0
11	11025.0
12	12608.0
13	13978.0
14	15767.0
15	16525.0
16	17344.0
17	18348.0
18	17387.0
19	16345.0
20	16150.0
21	16948.0
22	18932.0
23	22535.0
24	27305.0
25	34256.0
26	42018.0
27	49735.0
28	60208.0
29	76195.0
30	94083.0
31	112765.0
32	145406.0
33	195730.0
34	274563.0
35	403485.0
36	672065.0
37	1201788.0
38	1622061.0
39	600425.0
40	1772.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43386855537	20.29770887408868	13.895869802880004	23.37255276766132
2	15.510957566296424	20.136835197176488	40.540638903037504	23.81156833348959
3	18.41448250484621	25.055053389628394	30.363441262016565	26.167022843508832
4	12.588775240479766	16.582101247717304	38.94836310062916	31.88076041117377
5	13.068940120888376	37.88520652952208	35.739276008218006	13.306577341371536
6	31.168554752590943	37.83403505847076	17.572943996782403	13.424466192155895
7	27.124721187814128	32.50308980674892	22.56802564493479	17.804163360502166
8	24.797337708318587	36.705917198864476	20.49130699777371	18.00543809504322
9	24.75329441145048	16.88435974285393	21.66883303116043	36.69351281453516
10-11	23.81345004837037	27.037945580987827	29.144044071376147	20.004560299265655
12-13	24.034448317879196	24.585924979852454	29.182324167074093	22.19730253519426
14-15	21.54781441958951	26.86109142749324	26.186746053376382	25.40434809954087
16-17	21.232670345222463	30.197026435975527	26.923264954884008	21.647038263918
18-19	21.15325914170775	29.207241784261974	28.79131014065146	20.848188933378818
20-21	22.155446739079483	28.44582192055946	28.406584592250027	20.992146748111026
22-23	21.866361441078926	28.220022051648623	28.4636417824736	21.449974724798846
24-25	21.68984451387736	28.365366392792396	28.375647160042867	21.56914193328738
26-27	21.43583578641178	28.389063720110236	28.44969187814126	21.72540861533673
28-29	21.47348514325429	28.3973501914619	28.57432163184037	21.554843033443444
30-31	21.728655618468704	28.716817209307237	28.16089650368861	21.393630668535447
32-33	21.453696092932002	28.53031243495865	28.483034029215336	21.53295744289401
34-35	21.649665994891286	28.209064950893143	28.555971400532172	21.585297653683398
36-37	21.83338250000713	28.496937047594628	28.126828551643708	21.54285190075454
38-39	21.668558989315226	28.633762613853815	28.24733467612772	21.45034372070324
40-41	21.578537225614607	28.36387484934269	28.44193615174131	21.61565177330139
42-43	21.525954790903842	28.416700523385035	28.354571872886574	21.70277281282455
44-45	21.59833947555086	28.489896639849654	28.303034810856065	21.608729073743422
46-47	21.621674200233702	28.521384611062917	28.202010563065834	21.65493062563755
48-49	21.544914412501264	28.44750100939037	28.247727666268503	21.75985691183987
50-51	21.584380506911536	28.410097644891913	28.14003440568436	21.865487442512194
52-53	21.534981020573017	28.389846746588194	28.252290239533977	21.82288199330481
54-55	21.65712317287082	28.644845683189544	28.08628170354341	21.61174944039622
56-57	21.580468025324357	28.422684857458673	28.09678075574265	21.90006636147432
58-59	21.470567345764476	28.131339630047563	28.374382278481225	22.023710745706733
60-61	21.688907985139807	28.40301228823622	28.091613704757652	21.816466021866326
62-63	21.523510557512886	28.475097471509876	28.0799705774231	21.921421393554137
64-65	21.55757390675392	28.255849073437272	28.188256724478627	21.99832029533018
66-67	21.51410885364757	28.241135543906836	28.10748384130036	22.13727176114523
68-69	21.788877489542486	28.386060784184657	27.981805581002604	21.843256145270253
70-71	21.7507238074043	28.168277349698073	28.07327906912586	22.00771977377177
72-73	21.60464287711742	28.298110638534794	28.166673807997917	21.930572676349865
74-75	21.67166702163876	28.310279712422293	28.077608080618354	21.94044518532059
76-77	21.72271947028233	28.3147750300631	27.934924674332823	22.027580825321746
78-79	21.77654838668833	28.336430429542546	28.027785736788164	21.859235446980957
80-81	21.76157235353939	28.174146110438798	28.077354854203506	21.986926681818304
82-83	21.662467943579358	28.229363647767435	28.197030517242883	21.91113789141032
84-85	21.725214916267745	28.11135220592249	28.08835982753536	22.075073050274405
86-87	21.84413668434646	28.25628117907367	27.97425083225953	21.925331304320338
88-89	21.731280671443624	28.120919488414327	28.067706179351493	22.080093660790563
90-91	21.699956169666574	28.031179492133308	28.078355588526833	22.190508749673285
92-93	21.829362074324834	28.159565229167082	27.952944771268108	22.05812792523997
94-95	21.739951105041982	27.922786048292053	28.13285575987385	22.204407086792113
96-97	21.78033919445299	27.915638099820367	28.084690482756542	22.219332222970102
98-99	21.79994518035579	27.984347063670416	27.986660553242533	22.22904720273126
100	21.881839358325166	27.82801049117228	28.0108264606208	22.279323689881753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1351.0
1	1107.5
2	1123.5
3	1432.0
4	1418.0
5	1202.0
6	982.0
7	911.0
8	888.5
9	891.0
10	823.5
11	723.0
12	752.0
13	851.5
14	977.5
15	1135.5
16	1360.5
17	1709.0
18	2335.5
19	3305.0
20	4799.5
21	6986.0
22	10165.5
23	14718.5
24	20922.0
25	29139.0
26	40249.0
27	53776.0
28	69656.5
29	87927.5
30	106040.0
31	122741.0
32	137595.0
33	151287.5
34	164807.5
35	176566.5
36	187600.0
37	199015.0
38	209527.5
39	215452.0
40	218543.5
41	221454.5
42	221509.5
43	220121.0
44	216941.0
45	212459.5
46	207707.5
47	199726.0
48	191457.0
49	185976.0
50	180160.0
51	172694.0
52	161863.0
53	151425.5
54	144110.5
55	136782.5
56	126983.0
57	115195.0
58	104206.5
59	94447.0
60	83318.0
61	70710.0
62	59720.5
63	50157.0
64	41492.5
65	33993.5
66	27230.0
67	20969.5
68	15916.5
69	12219.0
70	9129.5
71	6534.5
72	4786.5
73	3475.0
74	2413.5
75	1626.5
76	1116.0
77	784.5
78	532.5
79	360.0
80	241.5
81	159.5
82	131.5
83	111.0
84	82.0
85	67.0
86	54.0
87	43.5
88	37.0
89	26.5
90	21.5
91	20.0
92	18.5
93	12.5
94	12.5
95	15.0
96	16.0
97	16.0
98	15.5
99	19.0
100	41.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019027613509773236
2	0.04214574481371799
3	0.04393953745296533
4	0.04638714236259255
5	0.05005854972703337
6	0.04700742579859397
7	0.04472746506085903
8	0.044140710459236066
9	0.04434188346550679
10-11	0.04504598898745435
12-13	0.04610214727037568
14-15	0.044258061379560655
16-17	0.040896795733120536
18-19	0.04387247978420842
20-21	0.04326057855680161
22-23	0.044408941134263705
24-25	0.04065371168387674
26-27	0.04088841352452593
28-29	0.0414248748745812
30-31	0.04147516812614889
32-33	0.04127399511987816
34-35	0.041181790825337405
36-37	0.038625217203980204
38-39	0.03897726996495399
40-41	0.04012563254241607
42-43	0.040025046039280705
44-45	0.03890183008760246
46-47	0.03784567180468113
48-49	0.03784567180468113
50-51	0.039186825179819335
52-53	0.03980710861582075
54-55	0.038792861375872484
56-57	0.03718347732570664
58-59	0.038147431314087224
60-61	0.03753553008668042
62-63	0.03766964542419424
64-65	0.03456822824418715
66-67	0.035674679778676166
68-69	0.036839806773327474
70-71	0.038147431314087224
72-73	0.03477778345905249
74-75	0.03528909818332393
76-77	0.03588423499354151
78-79	0.03640393192640756
80-81	0.03659672272408368
82-83	0.03505439634267475
84-85	0.03637040309202911
86-87	0.036571576098299835
88-89	0.037233770577274325
90-91	0.03771155646716731
92-93	0.03603511474824456
94-95	0.037426561374950436
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5965015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.47176843545882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.80774441625398	47.2150787880587
2	13.923005681202719	17.117435623209424
3	4.252545612711513	7.8423449749748535
4	1.744113230719752	4.288548985760983
5	0.8861178968580148	2.7235617081085834
6	0.547030467267644	2.017615812661047
7	0.3522376598232417	1.5156870301231558
8	0.2522592181040399	1.2405456192801154
9	0.1831972956438805	1.0135315562240617
>10	0.9767927996584116	10.793940182324445
>50	0.05656754438133469	2.357187865055431
>100	0.018197104265771383	1.7748192433620313
>500	1.6377758088532095E-4	0.06985269113708832
>1k	2.7295528787760316E-5	0.02984991972012027
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.676441718922752E-5	0.0
5	0.0	1.676441718922752E-5	0.0	1.676441718922752E-5	0.0
6	0.0	1.676441718922752E-5	0.0	1.676441718922752E-5	0.0
7	0.0	1.676441718922752E-5	0.0	1.676441718922752E-5	0.0
8	0.0	1.676441718922752E-5	0.0	1.676441718922752E-5	0.0
9	0.0	1.676441718922752E-5	0.0	1.676441718922752E-5	0.0
10-11	0.0	3.352883437845504E-5	0.0	2.5146625783841283E-5	0.0
12-13	0.0	4.19110429730688E-5	0.0	3.352883437845504E-5	0.0
14-15	0.0	7.543987735152384E-5	0.0	5.867546016229632E-5	0.0
16-17	0.0	1.0058650313536512E-4	0.0	1.0896871172997889E-4	0.0
18-19	0.0	1.0896871172997889E-4	0.0	1.1735092032459265E-4	0.0
20-21	0.0	1.1735092032459265E-4	0.0	1.508797547030477E-4	0.0
22-23	0.0	1.3411533751382017E-4	0.0	2.5146625783841283E-4	0.0
24-25	0.0	1.676441718922752E-4	0.0	5.95136810217577E-4	0.0
26-27	0.0	1.676441718922752E-4	0.0	0.0014082110438951119	0.0
28-29	0.0	1.676441718922752E-4	0.0	0.004819769941902912	0.0
30-31	0.0	1.676441718922752E-4	0.0	0.015389734979710863	0.0
32-33	0.0	1.676441718922752E-4	0.0	0.03955564235798233	0.0
34-35	0.0	1.676441718922752E-4	0.0	0.07761086937752881	0.0
36-37	0.0	1.676441718922752E-4	0.0	0.13725028352820573	0.0
38-39	0.0	1.927907976761165E-4	0.0	0.21813859646622852	0.0
40-41	0.0	2.2631963205457155E-4	0.0	0.2997142505090096	8.38220859461376E-6
42-43	0.0	2.347018406491853E-4	0.0	0.38186827694481906	1.676441718922752E-5
44-45	0.0	2.347018406491853E-4	0.0	0.4808705426558022	1.676441718922752E-5
46-47	0.0	2.347018406491853E-4	0.0	0.5974754464154742	1.676441718922752E-5
48-49	0.0	2.347018406491853E-4	0.0	0.7193443771725637	1.676441718922752E-5
50-51	0.0	2.347018406491853E-4	0.0	0.8370138214237517	1.676441718922752E-5
52-53	0.0	2.5984846643302656E-4	0.0	0.9538031337725053	1.676441718922752E-5
54-55	0.0	2.6823067502764034E-4	0.0	1.0814306418340944	1.676441718922752E-5
56-57	0.0	2.8499509221686784E-4	0.0	1.2337940474583886	1.676441718922752E-5
58-59	0.0	2.8499509221686784E-4	0.0	1.3941540800819445	1.676441718922752E-5
60-61	0.0	2.8499509221686784E-4	0.0	1.5584202219105903	1.676441718922752E-5
62-63	0.0	2.8499509221686784E-4	0.0	1.7172379281527372	1.676441718922752E-5
64-65	0.0	2.8499509221686784E-4	0.0	1.8874386736663697	1.676441718922752E-5
66-67	0.0	2.933773008114816E-4	0.0	2.078695527169672	1.676441718922752E-5
68-69	0.0	3.185239265953229E-4	0.0	2.2853253512354956	1.676441718922752E-5
70-71	0.0	3.185239265953229E-4	0.0	2.504142906597888	1.676441718922752E-5
72-73	0.0	3.185239265953229E-4	0.0	2.7255757110417997	1.676441718922752E-5
74-75	0.0	3.185239265953229E-4	0.0	2.952306071317507	1.676441718922752E-5
76-77	0.0	3.185239265953229E-4	0.0	3.1977539033849873	1.676441718922752E-5
78-79	0.0	3.269061351899367E-4	0.0	3.468868058169175	1.676441718922752E-5
80-81	0.0	3.352883437845504E-4	0.0	3.7559000270745337	1.676441718922752E-5
82-83	0.0	3.352883437845504E-4	0.0	4.049679673898557	1.676441718922752E-5
84-85	0.0	3.352883437845504E-4	0.0	4.343316823176471	1.676441718922752E-5
86-87	0.0	3.352883437845504E-4	0.0	4.652025183507502	1.676441718922752E-5
88	0.0	3.352883437845504E-4	0.0	4.900222379994015	1.676441718922752E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5570	0.0	43.72548	1
GTATCAA	9270	0.0	34.38824	1
TCAACGC	9965	0.0	30.759247	4
ATCAACG	10120	0.0	30.427492	3
CAACGCA	10625	0.0	28.893042	5
AACGCAG	11270	0.0	27.276585	6
TATCAAC	11535	0.0	27.061749	2
GTACATG	15670	0.0	25.774141	1
TACATGG	16035	0.0	23.864967	2
ACGCAGA	13285	0.0	22.85598	7
ACATGGG	16305	0.0	22.518301	3
CGCAGAG	13480	0.0	22.141788	8
CATGGGG	11925	0.0	18.410427	4
GCAGAGT	15485	0.0	18.394598	9
GAGTACA	11955	0.0	17.894592	1
GTACGAC	505	0.0	17.687546	3
GTGGTAT	3610	0.0	17.4525	1
AGAGTAC	13025	0.0	16.960905	10-11
CAGAGTA	14680	0.0	16.377533	10-11
TACGTCG	265	8.766288E-6	15.966351	5
>>END_MODULE