FastQCFastQC Report
Wed 25 May 2016
SRR2049377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5965015
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126520.2121034062781066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122450.20528028848209098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71610.12004999149205828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91000.055.748711
GTATCAA160800.040.0580061
TCAACGC197700.032.043344
ATCAACG198350.031.9857183
CAACGCA204200.031.0693765
TATCAAC208600.030.6167932
AACGCAG214450.029.6336026
GTGGTAT44250.027.5195471
ACGCAGA248950.025.3948657
CGCAGAG251300.025.045088
GTACATG149950.025.021381
TGGTATC45900.024.3679372
TACATGG152750.023.3515172
GCAGAGT275650.022.4572549
ACATGGG153200.022.362653
GAGTACT193000.020.67059512-13
AGAGTAC250350.020.19852610-11
CAGAGTA269600.019.94275510-11
AGTACTT203450.018.88090512-13
GTACTTT214200.018.50632314-15
Produced by FastQC (version 0.11.5)