##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049377_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5965015
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.877163259438575	31.0	31.0	34.0	30.0	34.0
2	32.0579322935483	33.0	31.0	34.0	30.0	34.0
3	32.039643320259884	34.0	31.0	34.0	30.0	34.0
4	35.721290726008235	37.0	35.0	37.0	33.0	37.0
5	35.81300415841368	37.0	35.0	37.0	35.0	37.0
6	35.86074485982014	37.0	35.0	37.0	35.0	37.0
7	35.79623655598519	37.0	35.0	37.0	35.0	37.0
8	35.8027322647135	37.0	35.0	37.0	35.0	37.0
9	37.554291313601055	39.0	37.0	39.0	35.0	39.0
10-11	37.51246493093479	39.0	37.0	39.0	35.0	39.0
12-13	37.46235089098686	39.0	37.0	39.0	34.5	39.0
14-15	38.76198727077803	40.0	38.0	41.0	35.0	41.0
16-17	38.78752132224311	40.0	38.0	41.0	35.0	41.0
18-19	38.85079735759256	40.0	38.0	41.0	35.0	41.0
20-21	38.879740704759335	40.0	38.0	41.0	35.0	41.0
22-23	38.84980800551214	40.0	38.0	41.0	35.0	41.0
24-25	38.829464972007614	40.0	38.0	41.0	35.0	41.0
26-27	38.71942845072476	40.0	38.0	41.0	34.5	41.0
28-29	38.642248343046916	40.0	38.0	41.0	34.0	41.0
30-31	38.54949140949353	40.0	38.0	41.0	34.0	41.0
32-33	38.425228519961806	40.0	38.0	41.0	34.0	41.0
34-35	38.333411986390644	40.0	38.0	41.0	34.0	41.0
36-37	38.23221634815671	40.0	38.0	41.0	34.0	41.0
38-39	38.1490943442724	40.0	38.0	41.0	33.5	41.0
40-41	38.04492453078492	40.0	38.0	41.0	33.0	41.0
42-43	37.92044261414263	40.0	38.0	41.0	33.0	41.0
44-45	37.756579153614865	40.0	37.0	41.0	33.0	41.0
46-47	37.54633508884722	40.0	37.0	41.0	33.0	41.0
48-49	37.348647069621784	40.0	37.0	41.0	32.0	41.0
50-51	37.20991296752816	40.0	37.0	41.0	32.0	41.0
52-53	37.07353351165085	40.0	36.5	41.0	31.5	41.0
54-55	36.93283470368473	40.0	36.0	41.0	31.0	41.0
56-57	36.7908065612576	39.0	36.0	41.0	31.0	41.0
58-59	36.60299814837012	39.0	35.0	41.0	31.0	41.0
60-61	36.425965064631015	39.0	35.0	41.0	31.0	41.0
62-63	36.22067505278696	39.0	35.0	41.0	31.0	41.0
64-65	35.994053745045065	38.5	35.0	40.5	30.0	41.0
66-67	35.72392693061124	38.0	35.0	40.0	30.0	41.0
68-69	35.43355490304718	37.0	35.0	40.0	30.0	41.0
70-71	35.10243109195869	37.0	34.5	39.5	29.0	41.0
72-73	34.8025050230385	36.0	34.0	39.0	29.0	41.0
74-75	34.49113891582837	36.0	34.0	39.0	29.0	41.0
76-77	34.16515507505011	35.0	34.0	38.5	29.0	40.0
78-79	33.82706430746612	35.0	34.0	37.0	29.0	39.0
80-81	33.509099809472396	35.0	34.0	37.0	29.0	39.0
82-83	33.20811540289505	35.0	34.0	36.5	28.5	39.0
84-85	32.93683846561996	35.0	34.0	36.0	28.0	37.0
86-87	32.70396897576955	35.0	33.5	36.0	28.0	37.0
88-89	32.47945604495546	35.0	33.0	35.0	27.0	37.0
90-91	32.28843774910876	35.0	33.0	35.0	27.0	36.0
92-93	32.108022947134245	35.0	33.0	35.0	27.0	36.0
94-95	31.951918813280436	35.0	33.0	35.0	26.5	36.0
96-97	31.75329743512799	35.0	33.0	35.0	26.0	36.0
98-99	31.608222611342974	35.0	33.0	35.0	25.5	35.0
100	31.4973196211577	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22566839417332218
1101	2	0.24117805309680307
1101	3	0.38868461154444844
1101	4	0.24218254093296565
1101	5	0.20019554095533465
1101	6	0.18864067011648444
1101	7	0.06925649982431281
1101	8	0.1585771560208613
1101	9	0.22743571110901684
1101	10-11	0.26691665570660916
1101	12-13	0.25013805867254035
1101	14-15	0.3318429586009799
1101	16-17	0.21367786502994335
1101	18-19	0.35073662398588823
1101	20-21	0.2827692312894712
1101	22-23	0.2512777527324346
1101	24-25	0.2972885458087191
1101	26-27	0.3469478900430687
1101	28-29	0.3544983791429033
1101	30-31	0.33713663578668474
1101	32-33	0.3772749506511346
1101	34-35	0.4378175626561003
1101	36-37	0.4114486794281689
1101	38-39	0.4462898915084068
1101	40-41	0.4058840534674246
1101	42-43	0.47955804047467865
1101	44-45	0.47861968491679363
1101	46-47	0.4554507200433946
1101	48-49	0.457059576681381
1101	50-51	0.4019423490780909
1101	52-53	0.4807432908928533
1101	54-55	0.46307196775779147
1101	56-57	0.4039636956785131
1101	58-59	0.40746652776310555
1101	60-61	0.4333780152068343
1101	62-63	0.40854007603708453
1101	64-65	0.3473964145508006
1101	66-67	0.34554071914755013
1101	68-69	0.3996233658277788
1101	70-71	0.39180438802643636
1101	72-73	0.35414483052668544
1101	74-75	0.38130661311777203
1101	76-77	0.37002202089581004
1101	78-79	0.3887597385338708
1101	80-81	0.4368486431716718
1101	82-83	0.38408036990605865
1101	84-85	0.41565191334341023
1101	86-87	0.3642892335317356
1101	88-89	0.43040631187103884
1101	90-91	0.4215310885030945
1101	92-93	0.4625820111947121
1101	94-95	0.4447502208718497
1101	96-97	0.4815136927914523
1101	98-99	0.47346399607153344
1101	100	0.4970231191840817
1102	1	0.15512397165645453
1102	2	0.10205794667480461
1102	3	0.2898568206767038
1102	4	0.14748660177323814
1102	5	0.1755200621894062
1102	6	0.179201114812237
1102	7	0.14032333815151787
1102	8	0.12001280034336048
1102	9	0.11143149324366419
1102	10-11	0.17947389715605766
1102	12-13	0.1737701183150122
1102	14-15	0.24865042645886604
1102	16-17	0.22143049721849906
1102	18-19	0.2665208788646183
1102	20-21	0.18028410600022227
1102	22-23	0.21777401028390386
1102	24-25	0.21444193423340607
1102	26-27	0.2580770745102967
1102	28-29	0.23760137383027313
1102	30-31	0.2156995150328882
1102	32-33	0.20724225357098192
1102	34-35	0.22312421654388714
1102	36-37	0.24250250493896885
1102	38-39	0.24813189938360125
1102	40-41	0.2349646055070238
1102	42-43	0.24103177761667638
1102	44-45	0.2876449101840848
1102	46-47	0.2352318483967224
1102	48-49	0.14332361374412272
1102	50-51	0.2230253121760768
1102	52-53	0.2801507706157125
1102	54-55	0.17315344357056262
1102	56-57	0.20757687097670896
1102	58-59	0.17015325060157238
1102	60-61	0.16755987526345706
1102	62-63	0.19193295232300045
1102	64-65	0.1885103722980972
1102	66-67	0.13581256092644622
1102	68-69	0.18706168616340335
1102	70-71	0.18980877479671676
1102	72-73	0.21864355682590997
1102	74-75	0.18255658393855612
1102	76-77	0.19082347329977978
1102	78-79	0.19972526661379675
1102	80-81	0.1733683719662551
1102	82-83	0.23629521757120386
1102	84-85	0.18212041904179443
1102	86-87	0.19104424273006515
1102	88-89	0.20333700717501557
1102	90-91	0.23170222815193142
1102	92-93	0.17984146329150974
1102	94-95	0.19483475511279025
1102	96-97	0.21077446460668625
1102	98-99	0.218727417136801
1102	100	0.21453206289339377
1103	1	0.1712100600577493
1103	2	0.1705612089330799
1103	3	0.34395326416830585
1103	4	0.20101557906864542
1103	5	0.18652078153667873
1103	6	0.13798424307545076
1103	7	0.14026906862305566
1103	8	0.153560767727285
1103	9	0.18037369605429632
1103	10-11	0.1952465273654127
1103	12-13	0.2060412230608577
1103	14-15	0.23893193442136607
1103	16-17	0.2922250829483559
1103	18-19	0.2166358866681648
1103	20-21	0.2211612623788497
1103	22-23	0.27031281639391125
1103	24-25	0.25068994512920995
1103	26-27	0.24677029993493704
1103	28-29	0.31089262681091867
1103	30-31	0.2595883188356609
1103	32-33	0.277507208902648
1103	34-35	0.2381469817193178
1103	36-37	0.24968650808246196
1103	38-39	0.28614209877103747
1103	40-41	0.28803607114382146
1103	42-43	0.3404799666365719
1103	44-45	0.36271430412338646
1103	46-47	0.35456705941233224
1103	48-49	0.28369088567975353
1103	50-51	0.2722773580410518
1103	52-53	0.3012049838080557
1103	54-55	0.2549442191095892
1103	56-57	0.22915019754593402
1103	58-59	0.31028417973156763
1103	60-61	0.31869269858967186
1103	62-63	0.22561851858178272
1103	64-65	0.2908378471457098
1103	66-67	0.2218569266198358
1103	68-69	0.2314933834181403
1103	70-71	0.29240274218800977
1103	72-73	0.21682363087819567
1103	74-75	0.21587282298379762
1103	76-77	0.14611342177345676
1103	78-79	0.14910473404768965
1103	80-81	0.2089344892093834
1103	82-83	0.1694290153143072
1103	84-85	0.193558932402091
1103	86-87	0.16860050879400035
1103	88-89	0.2091233080965793
1103	90-91	0.27052403583963525
1103	92-93	0.29857550551642476
1103	94-95	0.273732981770312
1103	96-97	0.3087835344405576
1103	98-99	0.3572069757559575
1103	100	0.35621508072080843
1104	1	0.1419018573407591
1104	2	0.15572872469989818
1104	3	0.26832401080833534
1104	4	0.168766097157949
1104	5	0.14210238289638255
1104	6	0.14825080378420807
1104	7	0.16409606433040125
1104	8	0.11698586099250718
1104	9	0.14431059573922056
1104	10-11	0.18621813942405652
1104	12-13	0.1847679721400013
1104	14-15	0.2635163880235538
1104	16-17	0.2677553626711031
1104	18-19	0.23860900010815556
1104	20-21	0.28583834000051667
1104	22-23	0.24787715060347892
1104	24-25	0.23614843795414941
1104	26-27	0.25076207250076976
1104	28-29	0.3497879773720811
1104	30-31	0.3368541096149258
1104	32-33	0.3209068245890663
1104	34-35	0.29142078036804264
1104	36-37	0.3014833304321769
1104	38-39	0.3448861934624077
1104	40-41	0.31366116999502935
1104	42-43	0.37892668944758157
1104	44-45	0.3281688103419995
1104	46-47	0.36721926067502864
1104	48-49	0.35375594948455813
1104	50-51	0.2891608888095192
1104	52-53	0.2655993637909333
1104	54-55	0.2714725313065003
1104	56-57	0.29313058772641654
1104	58-59	0.3262411931585376
1104	60-61	0.3263636599887363
1104	62-63	0.297307365679238
1104	64-65	0.2435016191458459
1104	66-67	0.31544980230461306
1104	68-69	0.26167457112438797
1104	70-71	0.254032073256667
1104	72-73	0.2877988119397017
1104	74-75	0.3074062839193914
1104	76-77	0.24716522822867404
1104	78-79	0.25925779379227265
1104	80-81	0.2635054820955389
1104	82-83	0.27639981283091686
1104	84-85	0.29900426641248146
1104	86-87	0.28292708740912076
1104	88-89	0.28912200685277156
1104	90-91	0.22936303845401085
1104	92-93	0.35229867186990305
1104	94-95	0.3337048225960082
1104	96-97	0.3275762019047974
1104	98-99	0.33734989031929175
1104	100	0.34546068812155895
1105	1	0.2545383265709482
1105	2	0.1640370556118711
1105	3	0.28802732522955665
1105	4	0.18303790512619855
1105	5	0.14821299146376532
1105	6	0.12981249659725336
1105	7	0.1495414020704473
1105	8	0.1950284126794557
1105	9	0.15868161341133913
1105	10-11	0.20158576366693381
1105	12-13	0.1775830157537186
1105	14-15	0.2615474055867253
1105	16-17	0.22078301106143527
1105	18-19	0.21788313967146422
1105	20-21	0.23555985905552035
1105	22-23	0.21970383019730377
1105	24-25	0.2124379996543695
1105	26-27	0.2679671447401262
1105	28-29	0.2522354044515396
1105	30-31	0.25321937852024234
1105	32-33	0.2589194915689461
1105	34-35	0.2769910745416482
1105	36-37	0.20985700202169255
1105	38-39	0.24242186655849451
1105	40-41	0.16609683374829132
1105	42-43	0.1905032696261415
1105	44-45	0.25526420870762934
1105	46-47	0.2647405829242828
1105	48-49	0.22025924604238156
1105	50-51	0.20756634193602963
1105	52-53	0.2092193318855493
1105	54-55	0.2010341426139277
1105	56-57	0.1774356408381692
1105	58-59	0.23178921255667717
1105	60-61	0.20986384514934997
1105	62-63	0.20467868778973752
1105	64-65	0.205190511081625
1105	66-67	0.24429387127049296
1105	68-69	0.19621133966416693
1105	70-71	0.15662958681122063
1105	72-73	0.22164787042651568
1105	74-75	0.21254396160136935
1105	76-77	0.20501318089086595
1105	78-79	0.19432520962539002
1105	80-81	0.1908922527577488
1105	82-83	0.20464064517078384
1105	84-85	0.20449745195719515
1105	86-87	0.15577547535036018
1105	88-89	0.16089435108274586
1105	90-91	0.20411351358653462
1105	92-93	0.3070154033693342
1105	94-95	0.2791318539487442
1105	96-97	0.306841399490132
1105	98-99	0.29525673669847663
1105	100	0.3128121646939377
1106	1	0.14062043241722577
1106	2	0.12518480786342678
1106	3	0.21130794895154992
1106	4	0.14966676134847745
1106	5	0.06626027200903195
1106	6	0.11904747179927
1106	7	0.13583480899492884
1106	8	0.06891557991485797
1106	9	0.12346597838604367
1106	10-11	0.1171227051647179
1106	12-13	0.13048822885588152
1106	14-15	0.182022861387928
1106	16-17	0.16255837819245045
1106	18-19	0.18019544730464787
1106	20-21	0.14154536557176556
1106	22-23	0.1297854792517299
1106	24-25	0.11682802024584049
1106	26-27	0.18871689468940644
1106	28-29	0.19856933124258092
1106	30-31	0.19714145827322938
1106	32-33	0.1304098954553723
1106	34-35	0.16769010844878096
1106	36-37	0.18196885389846784
1106	38-39	0.2330753556895715
1106	40-41	0.23041891103213175
1106	42-43	0.21496251688334667
1106	44-45	0.23112756820660252
1106	46-47	0.2315361313343658
1106	48-49	0.26611858919800824
1106	50-51	0.2545210446718613
1106	52-53	0.2653430507924881
1106	54-55	0.2933016661270571
1106	56-57	0.23514909594681654
1106	58-59	0.3104820630473455
1106	60-61	0.2755756130751976
1106	62-63	0.2772445736423492
1106	64-65	0.22462688987317847
1106	66-67	0.24622988516620126
1106	68-69	0.2166265652494559
1106	70-71	0.2274086030998106
1106	72-73	0.25430360926741713
1106	74-75	0.22366215878291484
1106	76-77	0.21631233256029958
1106	78-79	0.2951009045155075
1106	80-81	0.273869891462482
1106	82-83	0.3194654772440231
1106	84-85	0.27565500412634947
1106	86-87	0.2664472401379996
1106	88-89	0.24959468168933086
1106	90-91	0.25980102514819947
1106	92-93	0.32857850765029184
1106	94-95	0.2893826145549703
1106	96-97	0.32551301533256805
1106	98-99	0.3663005638256607
1106	100	0.2965252153805089
1107	1	0.12141556422535515
1107	2	0.12550720363938694
1107	3	0.20445012971543264
1107	4	0.1315787001578954
1107	5	0.12163164116403635
1107	6	0.12669724572845809
1107	7	0.1033961198255895
1107	8	0.11295714530776735
1107	9	0.1355785128033844
1107	10-11	0.13362576116310265
1107	12-13	0.1743981831244028
1107	14-15	0.18815932714991845
1107	16-17	0.1399062305787524
1107	18-19	0.12696213425135028
1107	20-21	0.10211433794122371
1107	22-23	0.07601302359658746
1107	24-25	0.12356127282509277
1107	26-27	0.2503885285285321
1107	28-29	0.2396993700418193
1107	30-31	0.1563531845251802
1107	32-33	0.1873944717563063
1107	34-35	0.13847307102957984
1107	36-37	0.2085079821464575
1107	38-39	0.15018691158823572
1107	40-41	0.14699328714639393
1107	42-43	0.23469928451942934
1107	44-45	0.15598039784035223
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2216	78-79	-0.6483810409946642
2216	80-81	-0.8033172712062964
2216	82-83	-0.7056582635304238
2216	84-85	-0.8079455014863584
2216	86-87	-0.6839944556614839
2216	88-89	-1.018566961818422
2216	90-91	-0.8205460993308193
2216	92-93	-0.8744089956948216
2216	94-95	-0.7822582823355475
2216	96-97	-0.8269840167835341
2216	98-99	-0.949507367157338
2216	100	-0.9285021859815394
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	342.0
3	44.0
4	49.0
5	113.0
6	252.0
7	695.0
8	1629.0
9	2934.0
10	4187.0
11	5821.0
12	8313.0
13	11446.0
14	14010.0
15	17806.0
16	22397.0
17	30521.0
18	31380.0
19	17618.0
20	14025.0
21	13857.0
22	14952.0
23	16582.0
24	19548.0
25	24685.0
26	31924.0
27	41221.0
28	54200.0
29	71035.0
30	92576.0
31	119508.0
32	154533.0
33	202064.0
34	275608.0
35	398803.0
36	651157.0
37	1196004.0
38	1703997.0
39	696378.0
40	2801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66692382258925	20.454729935968174	14.29633754062784	23.58200870081474
2	15.533456328274111	20.383838095964553	39.91619467847105	24.166510897290284
3	17.7978794349854	25.244467154645406	30.869198295259658	26.08845511510954
4	12.505695715514308	17.080792823482273	38.22166266276167	32.19184879824174
5	12.62452818643373	37.84156452246977	35.80762160698674	13.726285684109762
6	30.886812522684355	38.2083196773185	17.094877380861572	13.809990419135577
7	26.635021035152466	32.92670680626956	22.3889629782993	18.049309180278676
8	24.893691605823815	36.5470027696763	20.180765762435506	18.378539862064386
9	24.668437547935756	16.45407429822054	21.552904728655335	37.324583425188365
10-11	23.813619915457043	27.0114241120936	28.802132098578127	20.372823873871234
12-13	24.286910722023254	24.49867288054818	29.22026794974028	21.994148447688282
14-15	21.582212065211294	26.5581471412577	26.687467974694762	25.17217281883625
16-17	20.76668883202212	29.970346258169194	27.44020152842881	21.82276338137988
18-19	20.623066590388536	28.40159178207925	30.22053180944717	20.75480981808504
20-21	21.647604673319126	27.566319659762573	30.061830564939445	20.724245101978855
22-23	21.307406603336286	27.403719856530117	30.141315654696594	21.147557885437003
24-25	21.20073257230061	27.543213056611737	30.08848185212142	21.167572518966235
26-27	20.958186075280167	27.567487098304355	30.13790168516887	21.33642514124661
28-29	21.0261913728897	27.523167021751977	30.29672178459056	21.153919820767765
30-31	21.27209235852718	27.835202426146456	29.904593701776104	20.98811151355026
32-33	20.96945082704024	27.66153127088221	30.194043392412347	21.174974509665205
34-35	21.1649293698221	27.383825084065183	30.20906572893207	21.24217981718065
36-37	21.392256698161706	27.694849307549763	29.704936962841742	21.207957031446785
38-39	21.28102441584446	27.83129319625257	29.787194449890563	21.1004879380124
40-41	21.185761931437312	27.622290898663433	29.876906294433613	21.315040875465645
42-43	21.168344541237055	27.65163612259339	29.74604973992399	21.433969596245557
44-45	21.284291701592622	27.77957250628667	29.55612740989103	21.380008382229672
46-47	21.384576749029797	27.921258097433917	29.252362989682997	21.441802163853286
48-49	21.385980445976056	27.89707024932181	29.05674244502668	21.66020685967546
50-51	21.433391215481524	27.92634829138135	28.855016450980504	21.785244042156624
52-53	21.437235681461424	28.001157756376777	28.85562756187756	21.70597900028424
54-55	21.475605403463444	28.27973362566134	28.693221395714225	21.551439575160995
56-57	21.398739008148716	28.023397201033294	28.736718118580978	21.841145672237005
58-59	21.275357673391607	27.702036996088353	29.012035019561576	22.010570310958457
60-61	21.470981287608417	28.01935857718129	28.73500766009481	21.774652475115484
62-63	21.267415013793872	28.10834861142591	28.73289513036784	21.891341244412377
64-65	21.277401331401393	27.845740975722855	28.868919041480858	22.0079386513949
66-67	21.266160815479882	27.882408333577118	28.75873889205229	22.09269195889071
68-69	21.541912744454407	27.985859314699486	28.59087202569156	21.881355915154547
70-71	21.52725796233389	27.82754408179325	28.621300518307574	22.02389743756529
72-73	21.339843690407157	27.94042773485207	28.6523104860605	22.06741808868027
74-75	21.432365011668143	27.942769104905985	28.514600265550815	22.110265617875058
76-77	21.441122673701287	27.897135184735493	28.411873344974065	22.249868796589155
78-79	21.450365912622225	27.93621995127424	28.520069031513483	22.093345104590053
80-81	21.398834723519055	27.848728144988716	28.496321911169442	22.25611522032279
82-83	21.280286634638994	27.87035522788934	28.6271517484946	22.222206388977067
84-85	21.371967146165098	27.726081352672427	28.48679697239358	22.4151545287689
86-87	21.379891673534583	27.8758249046259	28.42715558199155	22.317127839847963
88-89	21.24706732226877	27.743548360462462	28.505564679767815	22.503819637500957
90-91	21.140905758126127	27.640064957223977	28.530858746873456	22.688170537776443
92-93	21.272356556171218	27.7861210847998	28.391390847163077	22.55013151186591
94-95	21.11877059848811	27.538067778932984	28.55878293086167	22.784378691717233
96-97	21.104721614585777	27.591719386858692	28.536768226522696	22.766790772032838
98-99	21.065552044793208	27.624184952034703	28.482599596669345	22.827663406502747
100	21.148761946082818	27.4470798128929	28.46818217023983	22.93597607078445
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	649.0
1	490.0
2	555.5
3	811.0
4	561.5
5	367.5
6	901.0
7	2465.0
8	4123.0
9	4723.0
10	3777.5
11	2552.0
12	2435.5
13	2790.0
14	3300.0
15	3842.0
16	4433.5
17	5027.5
18	5867.0
19	6931.0
20	8376.5
21	10541.5
22	13515.0
23	17850.0
24	23751.0
25	31391.5
26	41781.5
27	54770.0
28	70088.0
29	87754.0
30	105427.5
31	121625.0
32	136215.5
33	149406.0
34	162904.0
35	174683.5
36	184655.5
37	195960.5
38	206749.5
39	212663.5
40	216292.5
41	219638.0
42	220651.5
43	220278.5
44	216596.0
45	211891.0
46	207531.0
47	199700.0
48	191026.0
49	185361.5
50	180212.0
51	172305.0
52	161251.0
53	150938.0
54	143312.0
55	135489.5
56	125386.5
57	113590.0
58	103411.5
59	93966.5
60	82377.5
61	69622.0
62	58944.5
63	49393.0
64	39636.5
65	32127.0
66	25938.0
67	19957.5
68	14922.5
69	11127.0
70	8169.5
71	5742.0
72	3983.0
73	2751.0
74	1902.5
75	1251.5
76	781.0
77	476.5
78	284.5
79	171.5
80	109.0
81	52.5
82	24.0
83	18.0
84	8.5
85	7.0
86	7.5
87	2.5
88	3.0
89	3.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03948020248063081
2	0.0
3	1.676441718922752E-5
4	8.38220859461376E-5
5	0.0
6	0.0
7	0.0
8	9.723361969751962E-4
9	0.0
10-11	0.0
12-13	0.04468555401788596
14-15	9.220429454075137E-5
16-17	8.38220859461376E-6
18-19	4.19110429730688E-5
20-21	6.705766875691008E-5
22-23	0.0
24-25	1.0896871172997887E-4
26-27	7.543987735152384E-5
28-29	2.766128836222541E-4
30-31	0.0
32-33	1.5087975470304767E-4
34-35	1.676441718922752E-5
36-37	2.514662578384128E-5
38-39	2.5984846643302656E-4
40-41	1.0896871172997887E-4
42-43	4.6940368129837055E-4
44-45	2.514662578384128E-4
46-47	1.3411533751382017E-4
48-49	8.968963196236725E-4
50-51	3.520527609737779E-4
52-53	4.945503070822119E-4
54-55	4.358748469199155E-4
56-57	4.6940368129837055E-4
58-59	1.0058650313536512E-4
60-61	1.7602638048688896E-4
62-63	7.879276078936935E-4
64-65	3.6881717816300547E-4
66-67	0.001433357669678953
68-69	0.0016596773017335247
70-71	4.023460125414605E-4
72-73	9.723361969751962E-4
74-75	9.220429454075138E-4
76-77	6.789588961637146E-4
78-79	0.003403176689413186
80-81	0.002757746627627927
82-83	0.0011651269946513125
84-85	0.002866715339357906
86-87	0.0013914466267058842
88-89	0.0019530546025450062
90-91	0.002791275462006382
92-93	0.002581720247141038
94-95	0.0037971404933600334
96-97	0.0032020036831424564
98-99	0.003051123928439409
100	0.0014920331298412493
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5965015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.47752061538408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.74213072037094	45.04954141373979
2	14.495988449685257	17.24370903713049
3	4.419796188893671	7.886355568221578
4	1.8735149804564852	4.45728103493326
5	0.9785706804052549	2.9101478908706984
6	0.5638910929695973	2.012330646415841
7	0.35489387592995425	1.4775745475328192
8	0.2658243778623042	1.2648459931501475
9	0.18621579054095974	0.9968088168728979
>10	1.0341015118530896	11.108342354559454
>50	0.06288947420205356	2.5346732898721642
>100	0.021422030278909352	2.1046161431569717
>500	4.2268447730313646E-4	0.18647258460870894
>1k	2.536065558496754E-4	0.23001254942045568
>5k	2.817850609319486E-5	0.12001747755489124
>10k+	5.635701218638972E-5	0.4172706519598
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12652	0.2121034062781066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12245	0.20528028848209098	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7161	0.12004999149205828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.676441718922752E-5	0.0	0.0	0.0
3	0.0	1.676441718922752E-5	0.0	0.0	0.0
4	0.0	1.676441718922752E-5	0.0	0.0	0.0
5	0.0	1.676441718922752E-5	0.0	1.676441718922752E-5	0.0
6	0.0	1.676441718922752E-5	0.0	3.352883437845504E-5	0.0
7	0.0	1.676441718922752E-5	0.0	6.705766875691008E-5	0.0
8	0.0	1.676441718922752E-5	0.0	6.705766875691008E-5	0.0
9	0.0	1.676441718922752E-5	0.0	8.38220859461376E-5	0.0
10-11	0.0	3.352883437845504E-5	0.0	1.0058650313536513E-4	0.0
12-13	0.0	3.352883437845504E-5	0.0	1.1735092032459265E-4	0.0
14-15	0.0	5.867546016229632E-5	0.0	1.1735092032459265E-4	0.0
16-17	0.0	8.38220859461376E-5	0.0	1.5926196329766145E-4	0.0
18-19	0.0	8.38220859461376E-5	0.0	1.7602638048688898E-4	0.0
20-21	0.0	8.38220859461376E-5	0.0	2.1793742345995777E-4	0.0
22-23	0.0	8.38220859461376E-5	0.0	3.185239265953229E-4	0.0
24-25	0.0	8.38220859461376E-5	0.0	6.621944789744871E-4	0.0
26-27	0.0	8.38220859461376E-5	0.0	0.0014752687126520219	0.0
28-29	0.0	8.38220859461376E-5	0.0	0.004870063193470595	0.0
30-31	0.0	8.38220859461376E-5	0.0	0.015515468108630071	0.0
32-33	0.0	8.38220859461376E-5	0.0	0.0398993129103615	0.0
34-35	0.0	8.38220859461376E-5	0.0	0.07793777551271874	0.0
36-37	0.0	9.220429454075135E-5	0.0	0.1376107184977741	0.0
38-39	0.0	1.0058650313536512E-4	0.0	0.21866667560768915	0.0
40-41	0.0	1.0058650313536512E-4	0.0	0.30053570695128173	0.0
42-43	0.0	1.1735092032459265E-4	0.0	0.38287414197617275	0.0
44-45	0.0	1.1735092032459265E-4	0.0	0.4818009678098043	0.0
46-47	0.0	1.1735092032459265E-4	0.0	0.5985316046983955	0.0
48-49	0.0	1.1735092032459265E-4	0.0	0.7208447925109995	0.0
50-51	0.0	1.3411533751382017E-4	0.0	0.8388327606887829	0.0
52-53	0.0	1.3411533751382017E-4	0.0	0.9563597073938623	0.0
54-55	0.0	1.3411533751382017E-4	0.0	1.084607498891453	0.0
56-57	0.0	1.4249754610843392E-4	0.0	1.2375157480743972	0.0
58-59	0.0	1.508797547030477E-4	0.0	1.3985212107597382	0.0
60-61	0.0	1.8440858908150273E-4	0.0	1.5634746936931423	0.0
62-63	0.0	1.8440858908150273E-4	0.0	1.7232060606721022	0.0
64-65	8.38220859461376E-6	1.8440858908150273E-4	0.0	1.8945635509717915	0.0
66-67	1.676441718922752E-5	2.0117300627073024E-4	0.0	2.08708611797288	0.0
68-69	1.676441718922752E-5	2.0117300627073024E-4	0.0	2.2947469536958414	0.0
70-71	1.676441718922752E-5	2.0117300627073024E-4	0.0	2.514578756298182	0.0
72-73	1.676441718922752E-5	2.1793742345995777E-4	0.0	2.7377299135039896	0.0
74-75	1.676441718922752E-5	2.4308404924379905E-4	0.0	2.965709222860294	0.0
76-77	1.676441718922752E-5	2.5146625783841283E-4	0.0	3.2128586432724813	0.0
78-79	1.676441718922752E-5	2.5146625783841283E-4	0.0	3.486110261248295	0.0
80-81	1.676441718922752E-5	2.5146625783841283E-4	0.0	3.7750533737132264	0.0
82-83	1.676441718922752E-5	2.6823067502764034E-4	0.0	4.071750029128175	0.0
84-85	1.676441718922752E-5	2.6823067502764034E-4	0.0	4.367373761843013	0.0
86-87	1.676441718922752E-5	2.6823067502764034E-4	0.0	4.678596784752427	0.0
88	1.676441718922752E-5	2.6823067502764034E-4	0.0	4.9287554180500806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9100	0.0	55.74871	1
GTATCAA	16080	0.0	40.058006	1
TCAACGC	19770	0.0	32.04334	4
ATCAACG	19835	0.0	31.985718	3
CAACGCA	20420	0.0	31.069376	5
TATCAAC	20860	0.0	30.616793	2
AACGCAG	21445	0.0	29.633602	6
GTGGTAT	4425	0.0	27.519547	1
ACGCAGA	24895	0.0	25.394865	7
CGCAGAG	25130	0.0	25.04508	8
GTACATG	14995	0.0	25.02138	1
TGGTATC	4590	0.0	24.367937	2
TACATGG	15275	0.0	23.351517	2
GCAGAGT	27565	0.0	22.457254	9
ACATGGG	15320	0.0	22.36265	3
GAGTACT	19300	0.0	20.670595	12-13
AGAGTAC	25035	0.0	20.198526	10-11
CAGAGTA	26960	0.0	19.942755	10-11
AGTACTT	20345	0.0	18.880905	12-13
GTACTTT	21420	0.0	18.506323	14-15
>>END_MODULE