FastQCFastQC Report
Wed 25 May 2016
SRR2049376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4448696
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.043.125611
GTATCAA71100.034.8444751
TCAACGC76450.031.1755124
ATCAACG78000.030.7368053
CAACGCA80400.029.5854135
AACGCAG84150.028.2634946
TATCAAC91400.026.7448582
GTACATG131050.026.1507071
TACATGG132500.024.3383662
ACGCAGA100350.023.5599947
ACATGGG134200.023.0492363
CGCAGAG101500.022.8299778
GTGGTAT26300.019.6620981
CATGGGG96300.019.3797154
GCAGAGT117100.018.825459
GAGTACA94400.018.575041
AGAGTAC99500.017.54951910-11
AGTACAT102000.016.130622
CAGAGTA112400.016.12083410-11
TATACTG26450.015.1069365
Produced by FastQC (version 0.11.5)