FastQCFastQC Report
Wed 25 May 2016
SRR2049376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4448696
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92150.20713935049731427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87790.1973387257749237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49740.11180804442470332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65150.056.211161
GTATCAA122450.040.6571271
TCAACGC149750.032.9208834
ATCAACG151400.032.531063
CAACGCA154150.032.011695
TATCAAC157600.031.2811032
AACGCAG159200.031.0540336
GTGGTAT28050.029.8322931
ACGCAGA186700.026.202787
CGCAGAG190600.025.518558
GTACATG125000.024.144851
TGGTATC33250.023.7454512
GTCGTAC2803.6379788E-1223.50551
TACATGG125650.023.002522
GCAGAGT209350.022.7162469
ACATGGG127450.022.1614113
GAGTACT140450.020.38712912-13
AGAGTAC191150.020.37578810-11
CAGAGTA203550.019.90868410-11
AGTACTT151600.018.60855112-13
Produced by FastQC (version 0.11.5)