FastQCFastQC Report
Wed 25 May 2016
SRR2049375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4286401
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39650.044.463011
GTATCAA70000.035.9307671
TCAACGC76350.032.38564
ATCAACG77950.031.7811643
CAACGCA80150.030.7915155
AACGCAG86150.028.7521156
TATCAAC89850.027.7812462
GTACATG116600.025.8446241
TACATGG121050.024.2323252
ACGCAGA102500.023.8445387
CGCAGAG104750.023.2426248
ACATGGG125000.022.6016273
CATGGGG86700.019.5190494
GCAGAGT118900.019.4883219
GAGTACA89100.017.9395681
AGAGTAC103250.017.89006210-11
CCGGTCG4405.456968E-1217.0913649
GTATAGG16050.016.988841
GAACCGT5050.016.7531176
TATAACG3652.6357156E-916.7427122
Produced by FastQC (version 0.11.5)