##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049375_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4286401
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.578332498522652	31.0	31.0	34.0	27.0	34.0
2	30.81759802687616	31.0	31.0	34.0	27.0	34.0
3	30.98307251234777	31.0	31.0	34.0	27.0	34.0
4	34.596214166616704	37.0	35.0	37.0	32.0	37.0
5	34.62580542511072	37.0	35.0	37.0	32.0	37.0
6	34.621075816285035	37.0	35.0	37.0	32.0	37.0
7	34.52880446789743	37.0	35.0	37.0	31.0	37.0
8	34.49135346879585	37.0	35.0	37.0	31.0	37.0
9	36.07641585563273	39.0	37.0	39.0	31.0	39.0
10-11	36.035134020358804	39.0	37.0	39.0	31.0	39.0
12-13	35.96719264016596	39.0	37.0	39.0	31.0	39.0
14-15	37.152400813642956	40.0	37.0	41.0	31.0	41.0
16-17	37.11204877938391	40.0	37.0	41.0	31.0	41.0
18-19	37.143319418785126	40.0	37.0	41.0	31.0	41.0
20-21	37.2100155818366	40.0	37.0	41.0	31.0	41.0
22-23	37.156706990316586	40.0	37.0	41.0	31.0	41.0
24-25	37.158393486750306	40.0	37.0	41.0	31.0	41.0
26-27	37.13753344589085	40.0	37.0	41.0	31.0	41.0
28-29	37.07651022384513	40.0	37.0	41.0	31.0	41.0
30-31	37.011412721301625	40.0	37.0	41.0	30.5	41.0
32-33	36.95079788381908	40.0	37.0	41.0	30.0	41.0
34-35	36.85408831324928	40.0	37.0	41.0	30.0	41.0
36-37	36.7801430617434	40.0	37.0	41.0	30.0	41.0
38-39	36.67817814992111	40.0	37.0	41.0	30.0	41.0
40-41	36.57373890123672	40.0	36.5	41.0	30.0	41.0
42-43	36.438340346598466	40.0	36.0	41.0	30.0	41.0
44-45	36.31925991058699	40.0	36.0	41.0	30.0	41.0
46-47	36.199168953161404	40.0	36.0	41.0	29.0	41.0
48-49	36.06900625956368	40.0	36.0	41.0	29.0	41.0
50-51	35.929732309226324	39.0	35.0	41.0	28.5	41.0
52-53	35.78577307629408	39.0	35.0	41.0	28.0	41.0
54-55	35.644757571678426	39.0	35.0	41.0	28.0	41.0
56-57	35.51796343832507	39.0	35.0	41.0	28.0	41.0
58-59	35.3115235835378	39.0	35.0	41.0	27.5	41.0
60-61	35.09469750963571	38.5	34.5	40.0	27.0	41.0
62-63	34.88512180731574	38.0	34.0	40.0	26.5	41.0
64-65	34.643053811344295	38.0	34.0	40.0	26.0	41.0
66-67	34.38118762103686	37.0	34.0	40.0	26.0	41.0
68-69	34.094033549357604	37.0	34.0	40.0	26.0	41.0
70-71	33.807078712420974	36.0	34.0	39.0	26.0	41.0
72-73	33.467790694337744	36.0	33.0	39.0	25.0	41.0
74-75	33.12079399477557	35.5	33.0	39.0	25.0	40.0
76-77	32.78786539103551	35.0	33.0	37.5	24.0	40.0
78-79	32.47825903362751	35.0	33.0	37.0	24.0	39.0
80-81	32.13423359130422	35.0	32.5	37.0	23.5	39.0
82-83	31.83119159873283	35.0	32.0	36.0	22.5	39.0
84-85	31.550202255925193	35.0	32.0	36.0	22.0	37.0
86-87	31.242425055425286	35.0	32.0	35.5	20.5	37.0
88-89	31.00823499714562	35.0	32.0	35.0	20.0	36.5
90-91	30.817145082786233	35.0	32.0	35.0	19.0	36.0
92-93	30.59250756053855	35.0	32.0	35.0	18.0	36.0
94-95	30.401984788637368	35.0	32.0	35.0	11.0	36.0
96-97	30.163277654143883	34.5	31.5	35.0	2.0	35.5
98-99	29.966165671387255	34.0	31.0	35.0	2.0	35.0
100	29.851445070118263	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28180753127546865
1101	2	0.2651487886345656
1101	3	0.3040508337198844
1101	4	0.31684228457617536
1101	5	0.322680117060024
1101	6	0.3627922617044419
1101	7	0.420299071432666
1101	8	0.4245537383610838
1101	9	0.5182604461948799
1101	10-11	0.532456741057814
1101	12-13	0.5877665474300429
1101	14-15	0.627750696375692
1101	16-17	0.6243994610885508
1101	18-19	0.6465658829950769
1101	20-21	0.6372878771604107
1101	22-23	0.638470777349049
1101	24-25	0.6568848589447782
1101	26-27	0.5528595477033065
1101	28-29	0.5807670611964681
1101	30-31	0.49399760187591824
1101	32-33	0.5747828644742015
1101	34-35	0.6107959445822573
1101	36-37	0.5611680583299901
1101	38-39	0.6124267510547767
1101	40-41	0.6900428036767892
1101	42-43	0.6862320910319752
1101	44-45	0.5682770760908582
1101	46-47	0.5298739873576181
1101	48-49	0.47060600042919276
1101	50-51	0.5762264889444566
1101	52-53	0.5388707880526269
1101	54-55	0.5213801262865161
1101	56-57	0.5805048663101999
1101	58-59	0.5965840292806632
1101	60-61	0.6311010790219314
1101	62-63	0.6284015331311039
1101	64-65	0.5709132345996153
1101	66-67	0.5526750720135354
1101	68-69	0.5317877209022015
1101	70-71	0.596032042506728
1101	72-73	0.6805759647431984
1101	74-75	0.5869136645638378
1101	76-77	0.5812803830312205
1101	78-79	0.5348235242568435
1101	80-81	0.5779799524198026
1101	82-83	0.6140704738049152
1101	84-85	0.5131041014930346
1101	86-87	0.5790757287518069
1101	88-89	0.5188682671064839
1101	90-91	0.5048009063364844
1101	92-93	0.5341545137889767
1101	94-95	0.5783285071317614
1101	96-97	0.5788940485333889
1101	98-99	0.7032156930427433
1101	100	0.7392683728038101
1102	1	0.20301771012727698
1102	2	0.31736306360863864
1102	3	0.3493853218835419
1102	4	0.43147564597904164
1102	5	0.37848267205534825
1102	6	0.5127835150131617
1102	7	0.5515233171006457
1102	8	0.5211471491513748
1102	9	0.6404785643716266
1102	10-11	0.6127803445984767
1102	12-13	0.6528209518498116
1102	14-15	0.719323987105291
1102	16-17	0.6748448345962998
1102	18-19	0.6497585454959136
1102	20-21	0.6089808066022613
1102	22-23	0.605502254487611
1102	24-25	0.6446352046060113
1102	26-27	0.6257448848568785
1102	28-29	0.6275355927645521
1102	30-31	0.5913620969327695
1102	32-33	0.6109798511789606
1102	34-35	0.6580315895258764
1102	36-37	0.6126221323251357
1102	38-39	0.6284822710449092
1102	40-41	0.6182156959845884
1102	42-43	0.6650782515549878
1102	44-45	0.5345123506020926
1102	46-47	0.4671393523563765
1102	48-49	0.5295024136982036
1102	50-51	0.6246561841082467
1102	52-53	0.5642290752287948
1102	54-55	0.5386963048878073
1102	56-57	0.568838582895502
1102	58-59	0.6682726054074593
1102	60-61	0.544660520334034
1102	62-63	0.5325451813106028
1102	64-65	0.5688847900924969
1102	66-67	0.5994252555605186
1102	68-69	0.5606405315813419
1102	70-71	0.6051859423425512
1102	72-73	0.5534477879248954
1102	74-75	0.5836564634997075
1102	76-77	0.6273814018404735
1102	78-79	0.592334301382337
1102	80-81	0.5802665111189143
1102	82-83	0.6311440418914387
1102	84-85	0.6421587003430673
1102	86-87	0.6514317375150576
1102	88-89	0.633430212776581
1102	90-91	0.7372240763350391
1102	92-93	0.6631155564266287
1102	94-95	0.696032718336447
1102	96-97	0.692971534336376
1102	98-99	0.7639510480769474
1102	100	0.7287438731212283
1103	1	0.039735176792447646
1103	2	0.15424588819574225
1103	3	0.20987567888681724
1103	4	0.25548427814634067
1103	5	0.2510740586831659
1103	6	0.3553240199842449
1103	7	0.34737200466928186
1103	8	0.2582967079550258
1103	9	0.4183630925621955
1103	10-11	0.3828551977500396
1103	12-13	0.37009915119107717
1103	14-15	0.4332112168109674
1103	16-17	0.39541302870766515
1103	18-19	0.36735997248383256
1103	20-21	0.38725580181855435
1103	22-23	0.40139039018794165
1103	24-25	0.3988660102979438
1103	26-27	0.3500153363071661
1103	28-29	0.36330274287197994
1103	30-31	0.3810042012489703
1103	32-33	0.4164574082542032
1103	34-35	0.3734360154775871
1103	36-37	0.3891352911205175
1103	38-39	0.4294537346832996
1103	40-41	0.42121530309869826
1103	42-43	0.3556966160730184
1103	44-45	0.3272629574033985
1103	46-47	0.3641555142018973
1103	48-49	0.38228978317326323
1103	50-51	0.4123146366771806
1103	52-53	0.3536607637766096
1103	54-55	0.34691366723286876
1103	56-57	0.3192265538554935
1103	58-59	0.343562012649997
1103	60-61	0.3237700506835637
1103	62-63	0.37294421627498053
1103	64-65	0.4290812803873365
1103	66-67	0.4685884888930474
1103	68-69	0.46593223211644386
1103	70-71	0.4118251735584266
1103	72-73	0.39785071819284923
1103	74-75	0.401382240880082
1103	76-77	0.4681105350654349
1103	78-79	0.3287287874341658
1103	80-81	0.3102502511805838
1103	82-83	0.3797510313489809
1103	84-85	0.39709416855198754
1103	86-87	0.41521223339119473
1103	88-89	0.3989969138803744
1103	90-91	0.43708035441385107
1103	92-93	0.3770179941204539
1103	94-95	0.4676541286722582
1103	96-97	0.4299481183577072
1103	98-99	0.46713069615535474
1103	100	0.3673187009004124
1104	1	-0.07415308408447885
1104	2	-0.0056738008754528835
1104	3	0.05812022783377202
1104	4	0.11777294056800969
1104	5	0.19999509782328317
1104	6	0.14958878698817557
1104	7	0.24352731379327963
1104	8	0.1847962346436205
1104	9	0.2679195345636458
1104	10-11	0.31955983628104434
1104	12-13	0.2761698861670894
1104	14-15	0.36676991153505867
1104	16-17	0.33356676563961685
1104	18-19	0.3442258686417148
1104	20-21	0.29193930706212257
1104	22-23	0.24306851704134402
1104	24-25	0.3383924939006917
1104	26-27	0.299191153545479
1104	28-29	0.30053488669288697
1104	30-31	0.28012093749870104
1104	32-33	0.3081609460725261
1104	34-35	0.2910370995731242
1104	36-37	0.30871530609971387
1104	38-39	0.36296885643559307
1104	40-41	0.41329717809912836
1104	42-43	0.36348860227068513
1104	44-45	0.340618716953081
1104	46-47	0.2536269522674175
1104	48-49	0.2751079225618156
1104	50-51	0.2991740236272591
1104	52-53	0.31022596285607307
1104	54-55	0.34298024099007307
1104	56-57	0.2545948885648457
1104	58-59	0.34757681636911286
1104	60-61	0.28010339698975883
1104	62-63	0.2592885508924283
1104	64-65	0.26960115842658183
1104	66-67	0.3528342154985964
1104	68-69	0.34477231069435277
1104	70-71	0.329849088782062
1104	72-73	0.3743845932570622
1104	74-75	0.4082650621666346
1104	76-77	0.3740403758468247
1104	78-79	0.3347386143276623
1104	80-81	0.3495814932093353
1104	82-83	0.3143366122801794
1104	84-85	0.2852300950317108
1104	86-87	0.33165066186066383
1104	88-89	0.3605783523093393
1104	90-91	0.39541602731868153
1104	92-93	0.39509332545081577
1104	94-95	0.38227313799518825
1104	96-97	0.33314127942133354
1104	98-99	0.31525343884021595
1104	100	0.2809147851954954
1105	1	0.042737705099018086
1105	2	0.09869629177576655
1105	3	0.11870416528530825
1105	4	0.16618468476811898
1105	5	0.20968735918177117
1105	6	0.2746905563015787
1105	7	0.24198717402202163
1105	8	0.2486280158852452
1105	9	0.33924062664809895
1105	10-11	0.3110056648419004
1105	12-13	0.3398293840434832
1105	14-15	0.33779066379560874
1105	16-17	0.3713691982380425
1105	18-19	0.3454080906109809
1105	20-21	0.33025455834921047
1105	22-23	0.27238998380951074
1105	24-25	0.28969949977469156
1105	26-27	0.1987447345020641
1105	28-29	0.3353605994812199
1105	30-31	0.3142063416709746
1105	32-33	0.3418263487745179
1105	34-35	0.4487668245600318
1105	36-37	0.37783264138709427
1105	38-39	0.3107475511608513
1105	40-41	0.3428205709312877
1105	42-43	0.3133502064386846
1105	44-45	0.350377543955922
1105	46-47	0.31239711226685785
1105	48-49	0.2801056815737937
1105	50-51	0.3478962810254842
1105	52-53	0.3346840587513853
1105	54-55	0.3300608857306315
1105	56-57	0.2813739840593925
1105	58-59	0.33844551344206764
1105	60-61	0.29717467463751035
1105	62-63	0.22173644495654088
1105	64-65	0.2558823277243363
1105	66-67	0.2466816006568564
1105	68-69	0.17701619184848028
1105	70-71	0.25011383365328044
1105	72-73	0.21164454075390893
1105	74-75	0.23984063089145025
1105	76-77	0.1881476444737089
1105	78-79	0.16106614480628423
1105	80-81	0.18706575293628447
1105	82-83	0.17061856414517962
1105	84-85	0.17531058088739826
1105	86-87	0.2323007143875273
1105	88-89	0.1539052353914201
1105	90-91	0.19154428771911824
1105	92-93	0.2262768279846732
1105	94-95	0.18437312163242225
1105	96-97	0.18289830414808605
1105	98-99	0.1950706738167085
1105	100	0.2635930623144134
1106	1	0.12225099143621776
1106	2	0.13364820225902463
1106	3	0.19423968708380102
1106	4	0.27437995440090646
1106	5	0.22774291538124913
1106	6	0.3385633097480536
1106	7	0.3382615288296975
1106	8	0.3437244383219209
1106	9	0.42122452494729146
1106	10-11	0.41295544033143017
1106	12-13	0.395228600053386
1106	14-15	0.48848577695491
1106	16-17	0.5407861896354831
1106	18-19	0.5250369586934127
1106	20-21	0.4405321075340396
1106	22-23	0.5169994607708119
1106	24-25	0.5294159466444057
1106	26-27	0.4703405090226198
1106	28-29	0.4717802312237751
1106	30-31	0.404435488392096
1106	32-33	0.4556996122086332
1106	34-35	0.530843934691859
1106	36-37	0.4554528999508278
1106	38-39	0.4732279919756266
1106	40-41	0.5055897806590721
1106	42-43	0.4802322347059089
1106	44-45	0.4702039395255184
1106	46-47	0.423388829603887
1106	48-49	0.35851106611605843
1106	50-51	0.4644479763866798
1106	52-53	0.430366735166551
1106	54-55	0.46791784810513093
1106	56-57	0.44334165547899573
1106	58-59	0.5475167795791265
1106	60-61	0.47973532259999274
1106	62-63	0.4357111959318942
1106	64-65	0.4313470771465404
1106	66-67	0.5177425372259847
1106	68-69	0.514151104443556
1106	70-71	0.4617205960894424
1106	72-73	0.48546138100955716
1106	74-75	0.49336895722790786
1106	76-77	0.4753315158826368
1106	78-79	0.39628679559687896
1106	80-81	0.4974238338064154
1106	82-83	0.5061934245360575
1106	84-85	0.5016225820557381
1106	86-87	0.479103810476154
1106	88-89	0.49154756251140697
1106	90-91	0.539760003220195
1106	92-93	0.45195811352443727
1106	94-95	0.4802945418089237
1106	96-97	0.4260988243354866
1106	98-99	0.4650094521181245
1106	100	0.4353716442242259
1107	1	0.03855861088420198
1107	2	0.031265869046215755
1107	3	0.05371951611977721
1107	4	0.09674115395012706
1107	5	0.0603455942541018
1107	6	0.11379930255269244
1107	7	0.13762755995311693
1107	8	0.16571111667359162
1107	9	0.167131837994539
1107	10-11	0.04884939689964085
1107	12-13	0.13275076416315557
1107	14-15	0.0652238140343755
1107	16-17	0.1557655763808441
1107	18-19	0.12458691551291423
1107	20-21	0.100442389761632
1107	22-23	0.16192642184289952
1107	24-25	0.16711509310535888
1107	26-27	0.16416536111866264
1107	28-29	0.2199313433921688
1107	30-31	0.22034179344620242
1107	32-33	0.13503012923982283
1107	34-35	0.1694946225776519
1107	36-37	0.17172820070411632
1107	38-39	0.18475994642965077
1107	40-41	0.21494220638248862
1107	42-43	0.17898157325314656
1107	44-45	0.15048308022662837
1107	46-47	0.17035992646044207
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2216	2	-1.2372512780918754
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2216	4	-1.6012496171969417
2216	5	-1.522116263030476
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2216	54-55	-2.819661675049403
2216	56-57	-2.883175270757036
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2216	84-85	-4.049453675960379
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2216	88-89	-3.633286267163797
2216	90-91	-3.8374016570710126
2216	92-93	-3.85325243410184
2216	94-95	-3.7558046746769413
2216	96-97	-3.9885964464238945
2216	98-99	-3.735158440729876
2216	100	-3.351039263898514
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73180.0
3	13342.0
4	8454.0
5	6898.0
6	6219.0
7	6306.0
8	6895.0
9	7812.0
10	8999.0
11	9954.0
12	11230.0
13	12384.0
14	13705.0
15	14530.0
16	15207.0
17	15999.0
18	15277.0
19	13867.0
20	13974.0
21	14710.0
22	16193.0
23	19123.0
24	23255.0
25	28585.0
26	34782.0
27	40854.0
28	50125.0
29	62021.0
30	74756.0
31	88771.0
32	112847.0
33	149230.0
34	204455.0
35	294918.0
36	478987.0
37	836136.0
38	1094288.0
39	387246.0
40	887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85960286936092	20.099736495357927	14.096320011722725	22.94434062355843
2	15.093917778039682	20.046325846905326	40.77813420026069	24.081622174794305
3	18.15490700505951	25.496131327391247	30.37322836079045	25.97573330675879
4	12.460370658043123	16.79737970230849	39.24380774027797	31.498441899370413
5	12.900090020765226	38.209111912592334	35.78893736519933	13.10186070144311
6	30.800410021439305	38.219048725988145	17.689713101688024	13.29082815088453
7	26.828308562085112	32.82430971874833	22.659629909779802	17.687751809386757
8	24.742686931663165	36.85806512895351	20.534027452172946	17.865220487210372
9	24.75942575852769	17.025295303247706	21.68400568714893	36.531273251075675
10-11	23.71492762845356	27.20990905026793	29.17237832633309	19.90278499494542
12-13	24.02620779453866	24.71005124187235	29.280241380948098	21.983499582640896
14-15	21.43336001326567	26.99504649461514	26.302379594017705	25.269213898101484
16-17	21.072040646984576	30.3866828895514	26.996621969717566	21.54465449374646
18-19	21.05311124491806	29.404893600138166	28.90749350024972	20.634501654694056
20-21	22.04676621072194	28.5470067118605	28.572118718176775	20.834108359240787
22-23	21.743758056163202	28.32426204203126	28.653428439332586	21.278551462472954
24-25	21.54740371405924	28.514050741905173	28.522627410496042	21.415918133539545
26-27	21.333881152109093	28.510886672927455	28.591705889930903	21.56352628503255
28-29	21.416735727703397	28.50441683351393	28.733561003319362	21.345286435463308
30-31	21.627940545486307	28.787948625105326	28.32900666078471	21.255104168623657
32-33	21.338927757641642	28.618521275425124	28.624542473543197	21.418008493390044
34-35	21.520002987262128	28.397858973221997	28.666035763128782	21.416102276387093
36-37	21.72272826881191	28.62097396964592	28.25758659534432	21.398711166197856
38-39	21.56205199270843	28.79935271078032	28.32634802717513	21.312247269336122
40-41	21.508129822308696	28.524076274305393	28.504915980595126	21.462877922790785
42-43	21.431070233762476	28.552079917757805	28.484563835137532	21.532286013342187
44-45	21.532489115101797	28.615476421489582	28.347503664278033	21.504530799130585
46-47	21.58047135493394	28.706058069014205	28.210934104241847	21.50253647181001
48-49	21.559692227268272	28.57530256850674	28.260365275917277	21.604639928307716
50-51	21.598362917758134	28.566020928411955	28.201278407483137	21.63433774634677
52-53	21.56556449376061	28.549344450163478	28.25084374495687	21.634247311119044
54-55	21.636466788068287	28.798555689434956	28.10810438672461	21.45687313577215
56-57	21.53436874053824	28.60410225126505	28.162341631168914	21.699187377027794
58-59	21.513614431822823	28.315031009515806	28.393631234721322	21.777723323940045
60-61	21.68079909386305	28.608296352616748	28.12848344231224	21.58242111120796
62-63	21.506277839893787	28.692999031737042	28.139952620692732	21.660770507676443
64-65	21.563682837413094	28.455564022851664	28.24540627794193	21.73534686179331
66-67	21.440224855293977	28.4875242324099	28.164238738857527	21.908012173438596
68-69	21.758460126568615	28.588637995905508	28.106657425185723	21.546244452340154
70-71	21.718617396350293	28.453909924997202	28.1608275990505	21.66664507960201
72-73	21.552430173943655	28.55620803695228	28.231804456121097	21.65955733298297
74-75	21.599105734837075	28.570173386393378	28.149819752595196	21.68090112617435
76-77	21.664257134955946	28.55645130237231	27.984009577450987	21.79528198522076
78-79	21.746278655047494	28.568951517061613	28.119621189376492	21.565148638514405
80-81	21.689557551284828	28.456718830330363	28.13007069480581	21.723652923578992
82-83	21.631265670594075	28.509419625604654	28.225540337952754	21.633774365848513
84-85	21.639661109664218	28.425670774909197	28.14533526376859	21.789332851658
86-87	21.759143978587797	28.55714296716035	28.04093924130806	21.642773812943798
88-89	21.630933158335562	28.470338554872836	28.105695043205298	21.793033243586297
90-91	21.617499741830617	28.39011406711004	28.172201604325807	21.820184586733536
92-93	21.692454553378493	28.571176866011854	28.01445115808912	21.72191742252053
94-95	21.609003125086783	28.36915323940414	28.14525597543618	21.87658766007289
96-97	21.669752783279026	28.372823727878004	28.144975236801223	21.812448252041747
98-99	21.64253881053126	28.468825011938918	28.054666373958014	21.833969803571808
100	21.637826232310044	28.354836610013855	28.078497555408372	21.92883960226773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1496.0
1	1244.0
2	1141.5
3	1325.5
4	1221.0
5	999.5
6	819.5
7	707.0
8	712.5
9	728.0
10	656.5
11	604.5
12	662.5
13	757.5
14	861.5
15	968.0
16	1140.5
17	1467.0
18	1953.5
19	2609.5
20	3603.0
21	5277.0
22	7889.5
23	11707.0
24	16818.0
25	23557.5
26	32428.5
27	42907.5
28	54963.5
29	68328.5
30	81673.0
31	93233.5
32	102974.5
33	112516.5
34	121976.5
35	130059.0
36	136161.5
37	143198.0
38	150096.5
39	153353.0
40	155418.5
41	157026.0
42	156896.5
43	155652.5
44	152764.5
45	149572.0
46	146679.0
47	142129.0
48	136315.5
49	131685.0
50	128056.5
51	122766.5
52	114750.5
53	107114.5
54	100984.0
55	95298.5
56	88234.0
57	79653.0
58	72369.0
59	65908.5
60	57882.5
61	49073.0
62	41988.5
63	35339.5
64	28790.5
65	23415.5
66	18952.0
67	14710.0
68	11125.0
69	8562.0
70	6409.5
71	4742.0
72	3560.0
73	2518.0
74	1808.5
75	1261.0
76	809.0
77	557.0
78	386.0
79	267.5
80	191.5
81	124.5
82	107.5
83	93.5
84	67.0
85	52.5
86	47.5
87	40.0
88	25.5
89	20.0
90	16.5
91	14.5
92	13.0
93	11.5
94	13.0
95	11.5
96	9.0
97	9.0
98	10.0
99	10.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016517353369411774
2	0.03583425815736792
3	0.03728069305694917
4	0.03959032297724828
5	0.04278647751341977
6	0.0407568027349751
7	0.0387737871468395
8	0.0382605360534397
9	0.03856382079044868
10-11	0.0389954183008076
12-13	0.04016189805853442
14-15	0.03866880396864409
16-17	0.03516936469546363
18-19	0.03781727374550351
20-21	0.03728069305694917
22-23	0.03821387686313063
24-25	0.0354726494324726
26-27	0.035670950991286164
28-29	0.03602089491860421
30-31	0.03605588931133601
32-33	0.0359159117404088
34-35	0.0359509061331406
36-37	0.0336412762128415
38-39	0.03392123135469593
40-41	0.03494773354149554
42-43	0.03490107435118646
44-45	0.033839577771655055
46-47	0.03297638275093721
48-49	0.03315135471459623
50-51	0.03407287372320042
52-53	0.034679443197218364
54-55	0.033827912974077785
56-57	0.032381478074496525
58-59	0.03326800269036891
60-61	0.03260310922846463
62-63	0.032848069977587255
64-65	0.030013524166311083
66-67	0.031121679936151562
68-69	0.03204319894475575
70-71	0.03330299708310072
72-73	0.030480116069401816
74-75	0.03076007121125625
76-77	0.031168339126460637
78-79	0.03166992542228317
80-81	0.03177490860047859
82-83	0.03044512167667001
84-85	0.0316582606247059
86-87	0.0319148861714058
88-89	0.03252145564542375
90-91	0.03320967870248257
92-93	0.03141329987558327
94-95	0.03266143321635097
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4286401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.47887805321223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81793774705014	51.6091013414477
2	13.021238268278108	17.05232145340805
3	3.822346819251167	7.508489437644923
4	1.5858561169796597	4.153603171746072
5	0.8041394476335814	2.632707441468836
6	0.46911534697912	1.8430287958641682
7	0.3014969960260539	1.3819179525339855
8	0.21296940040237303	1.1155997918410168
9	0.14956682163991225	0.8814120907469725
>10	0.7644145135383571	8.764670662731334
>50	0.03809469994058853	1.6918732159398844
>100	0.01271682339299197	1.2835593819050108
>500	3.566629602105727E-5	0.01952385243937875
>1k	7.133259197404039E-5	0.062191410282772246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	4.665919030907281E-5	0.0	0.0	0.0
7	0.0	4.665919030907281E-5	0.0	0.0	0.0
8	0.0	4.665919030907281E-5	0.0	0.0	0.0
9	0.0	4.665919030907281E-5	0.0	0.0	0.0
10-11	0.0	4.665919030907281E-5	0.0	0.0	0.0
12-13	0.0	4.665919030907281E-5	0.0	2.3329595154536405E-5	0.0
14-15	0.0	4.665919030907281E-5	0.0	2.3329595154536405E-5	0.0
16-17	0.0	4.665919030907281E-5	0.0	2.3329595154536405E-5	0.0
18-19	0.0	4.665919030907281E-5	0.0	2.3329595154536405E-5	0.0
20-21	0.0	4.665919030907281E-5	0.0	6.998878546360922E-5	0.0
22-23	0.0	4.665919030907281E-5	0.0	1.8663676123629124E-4	0.0
24-25	0.0	4.665919030907281E-5	0.0	4.3159751035892345E-4	0.0
26-27	0.0	4.665919030907281E-5	0.0	7.232174497906285E-4	0.0
28-29	0.0	6.998878546360922E-5	0.0	0.0023212947178763723	0.0
30-31	0.0	6.998878546360922E-5	0.0	0.006718923404506485	0.0
32-33	0.0	8.165358304087742E-5	0.0	0.016785643713688944	0.0
34-35	0.0	9.331838061814562E-5	0.0	0.03224150050356931	0.0
36-37	0.0	9.331838061814562E-5	0.0	0.05580439160965109	0.0
38-39	0.0	9.331838061814562E-5	0.0	0.08826752326718848	0.0
40-41	0.0	9.331838061814562E-5	0.0	0.1215355259575574	0.0
42-43	0.0	9.331838061814562E-5	0.0	0.15388200963932214	0.0
44-45	0.0	1.0498317819541382E-4	0.0	0.193845606139043	0.0
46-47	0.0	1.1664797577268203E-4	0.0	0.24261612480960132	0.0
48-49	0.0	1.1664797577268203E-4	0.0	0.294967736336381	0.0
50-51	0.0	1.1664797577268203E-4	0.0	0.34369159581663034	0.0
52-53	0.0	1.1664797577268203E-4	0.0	0.3926254216532704	0.0
54-55	0.0	1.1664797577268203E-4	0.0	0.446668428828754	0.0
56-57	1.1664797577268203E-5	1.5164236850448665E-4	0.0	0.5092733974259525	0.0
58-59	2.3329595154536405E-5	1.8663676123629124E-4	0.0	0.5776174464311669	0.0
60-61	2.3329595154536405E-5	1.8663676123629124E-4	0.0	0.6463697633515857	0.0
62-63	2.3329595154536405E-5	1.8663676123629124E-4	0.0	0.7152620578429316	0.0
64-65	2.3329595154536405E-5	1.8663676123629124E-4	0.0	0.7878170987735398	0.0
66-67	2.3329595154536405E-5	1.8663676123629124E-4	0.0	0.8719086245080663	0.0
68-69	2.3329595154536405E-5	1.8663676123629124E-4	0.0	0.9639438773927125	0.0
70-71	2.3329595154536405E-5	1.8663676123629124E-4	0.0	1.063654567083201	0.0
72-73	2.3329595154536405E-5	1.8663676123629124E-4	0.0	1.1634469103567304	0.0
74-75	2.3329595154536405E-5	2.0996635639082765E-4	0.0	1.2657005259190637	0.0
76-77	2.3329595154536405E-5	2.3329595154536405E-4	0.0	1.3741714785900805	0.0
78-79	2.3329595154536405E-5	2.3329595154536405E-4	0.0	1.49736340580361	0.0
80-81	2.3329595154536405E-5	2.3329595154536405E-4	0.0	1.6279041554908185	0.0
82-83	2.3329595154536405E-5	2.3329595154536405E-4	0.0	1.7609994958474486	0.0
84-85	2.3329595154536405E-5	2.3329595154536405E-4	0.0	1.895529606306083	0.0
86-87	2.3329595154536405E-5	2.3329595154536405E-4	0.0	2.038749990959782	0.0
88	2.3329595154536405E-5	2.3329595154536405E-4	0.0	2.155957877016173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3965	0.0	44.46301	1
GTATCAA	7000	0.0	35.930767	1
TCAACGC	7635	0.0	32.3856	4
ATCAACG	7795	0.0	31.781164	3
CAACGCA	8015	0.0	30.791515	5
AACGCAG	8615	0.0	28.752115	6
TATCAAC	8985	0.0	27.781246	2
GTACATG	11660	0.0	25.844624	1
TACATGG	12105	0.0	24.232325	2
ACGCAGA	10250	0.0	23.844538	7
CGCAGAG	10475	0.0	23.242624	8
ACATGGG	12500	0.0	22.601627	3
CATGGGG	8670	0.0	19.519049	4
GCAGAGT	11890	0.0	19.488321	9
GAGTACA	8910	0.0	17.939568	1
AGAGTAC	10325	0.0	17.890062	10-11
CCGGTCG	440	5.456968E-12	17.091364	9
GTATAGG	1605	0.0	16.98884	1
GAACCGT	505	0.0	16.753117	6
TATAACG	365	2.6357156E-9	16.742712	2
>>END_MODULE